Amino acid dipepetide frequency for Cryobacterium sp. Hh39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.021AlaAla: 21.021 ± 0.234	0.719AlaCys: 0.719 ± 0.027	7.767AlaAsp: 7.767 ± 0.094	7.49AlaGlu: 7.49 ± 0.09	3.825AlaPhe: 3.825 ± 0.056	12.127AlaGly: 12.127 ± 0.113	2.455AlaHis: 2.455 ± 0.039	5.707AlaIle: 5.707 ± 0.07	2.485AlaLys: 2.485 ± 0.054	14.357AlaLeu: 14.357 ± 0.14	2.428AlaMet: 2.428 ± 0.047	2.644AlaAsn: 2.644 ± 0.05	6.429AlaPro: 6.429 ± 0.09	3.862AlaGln: 3.862 ± 0.056	8.564AlaArg: 8.564 ± 0.094	7.766AlaSer: 7.766 ± 0.086	8.321AlaThr: 8.321 ± 0.103	11.252AlaVal: 11.252 ± 0.11	1.729AlaTrp: 1.729 ± 0.041	2.203AlaTyr: 2.203 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.693CysAla: 0.693 ± 0.026	0.062CysCys: 0.062 ± 0.008	0.321CysAsp: 0.321 ± 0.018	0.274CysGlu: 0.274 ± 0.015	0.17CysPhe: 0.17 ± 0.011	0.653CysGly: 0.653 ± 0.024	0.136CysHis: 0.136 ± 0.011	0.21CysIle: 0.21 ± 0.012	0.08CysLys: 0.08 ± 0.008	0.499CysLeu: 0.499 ± 0.022	0.09CysMet: 0.09 ± 0.009	0.125CysAsn: 0.125 ± 0.009	0.331CysPro: 0.331 ± 0.02	0.14CysGln: 0.14 ± 0.011	0.368CysArg: 0.368 ± 0.019	0.375CysSer: 0.375 ± 0.017	0.388CysThr: 0.388 ± 0.017	0.462CysVal: 0.462 ± 0.018	0.101CysTrp: 0.101 ± 0.01	0.111CysTyr: 0.111 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.298AspAla: 8.298 ± 0.097	0.297AspCys: 0.297 ± 0.018	3.578AspAsp: 3.578 ± 0.078	3.558AspGlu: 3.558 ± 0.063	1.872AspPhe: 1.872 ± 0.039	5.206AspGly: 5.206 ± 0.073	1.138AspHis: 1.138 ± 0.032	2.318AspIle: 2.318 ± 0.044	1.006AspLys: 1.006 ± 0.031	6.194AspLeu: 6.194 ± 0.082	0.812AspMet: 0.812 ± 0.025	1.201AspAsn: 1.201 ± 0.035	3.722AspPro: 3.722 ± 0.063	1.661AspGln: 1.661 ± 0.037	4.016AspArg: 4.016 ± 0.057	3.111AspSer: 3.111 ± 0.048	3.351AspThr: 3.351 ± 0.056	4.923AspVal: 4.923 ± 0.068	0.913AspTrp: 0.913 ± 0.025	1.301AspTyr: 1.301 ± 0.032	0.002AspXaa: 0.002 ± 0.001
Glu	6.243GluAla: 6.243 ± 0.077	0.268GluCys: 0.268 ± 0.015	2.163GluAsp: 2.163 ± 0.05	2.273GluGlu: 2.273 ± 0.05	1.722GluPhe: 1.722 ± 0.038	3.291GluGly: 3.291 ± 0.054	1.366GluHis: 1.366 ± 0.035	2.687GluIle: 2.687 ± 0.054	1.305GluLys: 1.305 ± 0.04	6.175GluLeu: 6.175 ± 0.074	0.982GluMet: 0.982 ± 0.028	1.395GluAsn: 1.395 ± 0.042	2.844GluPro: 2.844 ± 0.043	2.136GluGln: 2.136 ± 0.041	4.154GluArg: 4.154 ± 0.066	2.965GluSer: 2.965 ± 0.053	3.227GluThr: 3.227 ± 0.054	3.976GluVal: 3.976 ± 0.054	0.798GluTrp: 0.798 ± 0.028	1.126GluTyr: 1.126 ± 0.033	0.001GluXaa: 0.001 ± 0.001
Phe	4.261PheAla: 4.261 ± 0.054	0.193PheCys: 0.193 ± 0.012	2.312PheAsp: 2.312 ± 0.041	1.727PheGlu: 1.727 ± 0.038	1.135PhePhe: 1.135 ± 0.035	3.473PheGly: 3.473 ± 0.061	0.58PheHis: 0.58 ± 0.019	1.294PheIle: 1.294 ± 0.036	0.503PheLys: 0.503 ± 0.02	2.926PheLeu: 2.926 ± 0.054	0.531PheMet: 0.531 ± 0.024	0.789PheAsn: 0.789 ± 0.03	1.435PhePro: 1.435 ± 0.035	0.842PheGln: 0.842 ± 0.026	1.722PheArg: 1.722 ± 0.039	2.009PheSer: 2.009 ± 0.04	2.484PheThr: 2.484 ± 0.05	2.824PheVal: 2.824 ± 0.05	0.5PheTrp: 0.5 ± 0.02	0.689PheTyr: 0.689 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.157GlyAla: 10.157 ± 0.109	0.652GlyCys: 0.652 ± 0.023	4.754GlyAsp: 4.754 ± 0.069	4.218GlyGlu: 4.218 ± 0.059	3.307GlyPhe: 3.307 ± 0.052	7.289GlyGly: 7.289 ± 0.106	1.801GlyHis: 1.801 ± 0.046	4.653GlyIle: 4.653 ± 0.065	2.125GlyLys: 2.125 ± 0.048	9.341GlyLeu: 9.341 ± 0.101	1.823GlyMet: 1.823 ± 0.041	1.958GlyAsn: 1.958 ± 0.047	3.844GlyPro: 3.844 ± 0.051	2.718GlyGln: 2.718 ± 0.05	5.529GlyArg: 5.529 ± 0.068	5.741GlySer: 5.741 ± 0.076	6.19GlyThr: 6.19 ± 0.081	7.577GlyVal: 7.577 ± 0.084	1.515GlyTrp: 1.515 ± 0.032	2.252GlyTyr: 2.252 ± 0.043	0.002GlyXaa: 0.002 ± 0.001
His	2.251HisAla: 2.251 ± 0.042	0.132HisCys: 0.132 ± 0.011	1.295HisAsp: 1.295 ± 0.034	1.117HisGlu: 1.117 ± 0.03	0.598HisPhe: 0.598 ± 0.019	1.872HisGly: 1.872 ± 0.045	0.513HisHis: 0.513 ± 0.023	0.745HisIle: 0.745 ± 0.023	0.34HisLys: 0.34 ± 0.016	2.086HisLeu: 2.086 ± 0.043	0.306HisMet: 0.306 ± 0.018	0.436HisAsn: 0.436 ± 0.017	1.494HisPro: 1.494 ± 0.037	0.559HisGln: 0.559 ± 0.019	1.515HisArg: 1.515 ± 0.036	1.132HisSer: 1.132 ± 0.03	1.167HisThr: 1.167 ± 0.031	1.523HisVal: 1.523 ± 0.034	0.275HisTrp: 0.275 ± 0.014	0.463HisTyr: 0.463 ± 0.019	0.001HisXaa: 0.001 ± 0.001
Ile	6.604IleAla: 6.604 ± 0.079	0.296IleCys: 0.296 ± 0.017	3.53IleAsp: 3.53 ± 0.052	2.781IleGlu: 2.781 ± 0.048	1.427IlePhe: 1.427 ± 0.037	4.589IleGly: 4.589 ± 0.073	0.729IleHis: 0.729 ± 0.025	2.047IleIle: 2.047 ± 0.049	0.859IleLys: 0.859 ± 0.028	3.912IleLeu: 3.912 ± 0.062	0.761IleMet: 0.761 ± 0.025	1.231IleAsn: 1.231 ± 0.029	2.421IlePro: 2.421 ± 0.043	1.017IleGln: 1.017 ± 0.028	2.773IleArg: 2.773 ± 0.052	2.632IleSer: 2.632 ± 0.048	3.238IleThr: 3.238 ± 0.055	4.742IleVal: 4.742 ± 0.075	0.532IleTrp: 0.532 ± 0.022	0.816IleTyr: 0.816 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.446LysAla: 2.446 ± 0.047	0.07LysCys: 0.07 ± 0.008	1.018LysAsp: 1.018 ± 0.032	0.847LysGlu: 0.847 ± 0.029	0.58LysPhe: 0.58 ± 0.022	1.389LysGly: 1.389 ± 0.035	0.44LysHis: 0.44 ± 0.019	1.05LysIle: 1.05 ± 0.031	0.782LysLys: 0.782 ± 0.029	2.008LysLeu: 2.008 ± 0.044	0.431LysMet: 0.431 ± 0.018	0.655LysAsn: 0.655 ± 0.025	1.242LysPro: 1.242 ± 0.031	0.668LysGln: 0.668 ± 0.024	1.582LysArg: 1.582 ± 0.043	1.293LysSer: 1.293 ± 0.031	1.479LysThr: 1.479 ± 0.037	1.6LysVal: 1.6 ± 0.039	0.278LysTrp: 0.278 ± 0.014	0.46LysTyr: 0.46 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	14.864LeuAla: 14.864 ± 0.125	0.576LeuCys: 0.576 ± 0.022	6.427LeuAsp: 6.427 ± 0.067	4.85LeuGlu: 4.85 ± 0.067	3.011LeuPhe: 3.011 ± 0.062	9.579LeuGly: 9.579 ± 0.098	2.016LeuHis: 2.016 ± 0.038	5.012LeuIle: 5.012 ± 0.081	1.888LeuLys: 1.888 ± 0.042	10.936LeuLeu: 10.936 ± 0.134	1.762LeuMet: 1.762 ± 0.039	2.323LeuAsn: 2.323 ± 0.034	5.668LeuPro: 5.668 ± 0.075	2.649LeuGln: 2.649 ± 0.043	6.981LeuArg: 6.981 ± 0.084	6.284LeuSer: 6.284 ± 0.079	7.24LeuThr: 7.24 ± 0.087	9.467LeuVal: 9.467 ± 0.113	1.227LeuTrp: 1.227 ± 0.036	1.714LeuTyr: 1.714 ± 0.04	0.002LeuXaa: 0.002 ± 0.001
Met	2.053MetAla: 2.053 ± 0.036	0.104MetCys: 0.104 ± 0.008	0.799MetAsp: 0.799 ± 0.025	0.647MetGlu: 0.647 ± 0.023	0.542MetPhe: 0.542 ± 0.021	1.262MetGly: 1.262 ± 0.034	0.332MetHis: 0.332 ± 0.016	0.92MetIle: 0.92 ± 0.029	0.534MetLys: 0.534 ± 0.021	1.997MetLeu: 1.997 ± 0.04	0.337MetMet: 0.337 ± 0.02	0.552MetAsn: 0.552 ± 0.021	1.126MetPro: 1.126 ± 0.032	0.554MetGln: 0.554 ± 0.02	1.254MetArg: 1.254 ± 0.036	1.497MetSer: 1.497 ± 0.031	1.805MetThr: 1.805 ± 0.036	1.358MetVal: 1.358 ± 0.033	0.187MetTrp: 0.187 ± 0.01	0.263MetTyr: 0.263 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.926AsnAla: 2.926 ± 0.051	0.136AsnCys: 0.136 ± 0.009	1.376AsnAsp: 1.376 ± 0.035	1.115AsnGlu: 1.115 ± 0.031	0.807AsnPhe: 0.807 ± 0.026	2.189AsnGly: 2.189 ± 0.046	0.432AsnHis: 0.432 ± 0.018	0.982AsnIle: 0.982 ± 0.031	0.478AsnLys: 0.478 ± 0.019	2.355AsnLeu: 2.355 ± 0.041	0.399AsnMet: 0.399 ± 0.018	0.634AsnAsn: 0.634 ± 0.027	1.762AsnPro: 1.762 ± 0.034	0.743AsnGln: 0.743 ± 0.023	1.54AsnArg: 1.54 ± 0.033	1.283AsnSer: 1.283 ± 0.029	1.497AsnThr: 1.497 ± 0.041	1.983AsnVal: 1.983 ± 0.039	0.369AsnTrp: 0.369 ± 0.019	0.567AsnTyr: 0.567 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	7.481ProAla: 7.481 ± 0.098	0.225ProCys: 0.225 ± 0.012	3.467ProAsp: 3.467 ± 0.05	3.46ProGlu: 3.46 ± 0.055	1.694ProPhe: 1.694 ± 0.036	5.087ProGly: 5.087 ± 0.074	1.084ProHis: 1.084 ± 0.032	2.272ProIle: 2.272 ± 0.048	1.032ProLys: 1.032 ± 0.028	4.946ProLeu: 4.946 ± 0.062	0.916ProMet: 0.916 ± 0.028	1.318ProAsn: 1.318 ± 0.03	2.257ProPro: 2.257 ± 0.053	1.504ProGln: 1.504 ± 0.032	3.187ProArg: 3.187 ± 0.056	3.152ProSer: 3.152 ± 0.053	3.933ProThr: 3.933 ± 0.074	4.914ProVal: 4.914 ± 0.072	0.765ProTrp: 0.765 ± 0.024	0.975ProTyr: 0.975 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.862GlnAla: 3.862 ± 0.055	0.141GlnCys: 0.141 ± 0.012	1.272GlnAsp: 1.272 ± 0.031	1.155GlnGlu: 1.155 ± 0.03	0.917GlnPhe: 0.917 ± 0.029	2.135GlnGly: 2.135 ± 0.043	0.657GlnHis: 0.657 ± 0.026	1.658GlnIle: 1.658 ± 0.035	0.713GlnLys: 0.713 ± 0.029	3.333GlnLeu: 3.333 ± 0.047	0.556GlnMet: 0.556 ± 0.021	0.801GlnAsn: 0.801 ± 0.024	1.566GlnPro: 1.566 ± 0.035	1.23GlnGln: 1.23 ± 0.032	2.245GlnArg: 2.245 ± 0.045	1.706GlnSer: 1.706 ± 0.037	1.692GlnThr: 1.692 ± 0.037	2.521GlnVal: 2.521 ± 0.046	0.436GlnTrp: 0.436 ± 0.022	0.629GlnTyr: 0.629 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.208ArgAla: 8.208 ± 0.095	0.336ArgCys: 0.336 ± 0.016	3.776ArgAsp: 3.776 ± 0.064	3.533ArgGlu: 3.533 ± 0.062	2.284ArgPhe: 2.284 ± 0.048	4.855ArgGly: 4.855 ± 0.064	1.491ArgHis: 1.491 ± 0.033	3.335ArgIle: 3.335 ± 0.053	1.406ArgLys: 1.406 ± 0.032	7.141ArgLeu: 7.141 ± 0.08	1.573ArgMet: 1.573 ± 0.033	1.421ArgAsn: 1.421 ± 0.034	3.455ArgPro: 3.455 ± 0.06	2.066ArgGln: 2.066 ± 0.04	5.46ArgArg: 5.46 ± 0.089	4.136ArgSer: 4.136 ± 0.061	4.079ArgThr: 4.079 ± 0.065	5.48ArgVal: 5.48 ± 0.067	0.987ArgTrp: 0.987 ± 0.027	1.478ArgTyr: 1.478 ± 0.033	0.002ArgXaa: 0.002 ± 0.001
Ser	7.922SerAla: 7.922 ± 0.091	0.295SerCys: 0.295 ± 0.017	3.219SerAsp: 3.219 ± 0.061	2.874SerGlu: 2.874 ± 0.041	2.05SerPhe: 2.05 ± 0.041	6.04SerGly: 6.04 ± 0.074	1.065SerHis: 1.065 ± 0.032	2.899SerIle: 2.899 ± 0.046	1.132SerLys: 1.132 ± 0.034	5.804SerLeu: 5.804 ± 0.065	1.263SerMet: 1.263 ± 0.035	1.42SerAsn: 1.42 ± 0.029	3.303SerPro: 3.303 ± 0.051	1.59SerGln: 1.59 ± 0.033	3.754SerArg: 3.754 ± 0.061	3.71SerSer: 3.71 ± 0.07	4.278SerThr: 4.278 ± 0.069	5.425SerVal: 5.425 ± 0.06	0.904SerTrp: 0.904 ± 0.029	1.301SerTyr: 1.301 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	8.778ThrAla: 8.778 ± 0.094	0.305ThrCys: 0.305 ± 0.016	4.258ThrAsp: 4.258 ± 0.065	3.375ThrGlu: 3.375 ± 0.058	2.058ThrPhe: 2.058 ± 0.045	6.406ThrGly: 6.406 ± 0.076	1.25ThrHis: 1.25 ± 0.035	3.282ThrIle: 3.282 ± 0.057	1.29ThrLys: 1.29 ± 0.032	6.997ThrLeu: 6.997 ± 0.087	1.05ThrMet: 1.05 ± 0.03	1.544ThrAsn: 1.544 ± 0.035	4.326ThrPro: 4.326 ± 0.058	1.661ThrGln: 1.661 ± 0.038	3.934ThrArg: 3.934 ± 0.059	3.913ThrSer: 3.913 ± 0.062	4.607ThrThr: 4.607 ± 0.072	6.609ThrVal: 6.609 ± 0.084	0.865ThrTrp: 0.865 ± 0.026	1.262ThrTyr: 1.262 ± 0.03	0.002ThrXaa: 0.002 ± 0.001
Val	11.27ValAla: 11.27 ± 0.109	0.543ValCys: 0.543 ± 0.017	5.232ValAsp: 5.232 ± 0.067	4.227ValGlu: 4.227 ± 0.059	2.929ValPhe: 2.929 ± 0.056	7.117ValGly: 7.117 ± 0.077	1.691ValHis: 1.691 ± 0.035	4.461ValIle: 4.461 ± 0.064	1.685ValLys: 1.685 ± 0.041	9.405ValLeu: 9.405 ± 0.097	1.47ValMet: 1.47 ± 0.036	2.146ValAsn: 2.146 ± 0.038	4.668ValPro: 4.668 ± 0.065	2.413ValGln: 2.413 ± 0.037	5.301ValArg: 5.301 ± 0.065	5.386ValSer: 5.386 ± 0.064	6.714ValThr: 6.714 ± 0.083	8.078ValVal: 8.078 ± 0.1	1.081ValTrp: 1.081 ± 0.029	1.538ValTyr: 1.538 ± 0.039	0.001ValXaa: 0.001 ± 0.001
Trp	1.538TrpAla: 1.538 ± 0.031	0.096TrpCys: 0.096 ± 0.009	0.682TrpAsp: 0.682 ± 0.022	0.567TrpGlu: 0.567 ± 0.023	0.546TrpPhe: 0.546 ± 0.021	0.994TrpGly: 0.994 ± 0.029	0.331TrpHis: 0.331 ± 0.015	0.689TrpIle: 0.689 ± 0.022	0.301TrpLys: 0.301 ± 0.016	1.78TrpLeu: 1.78 ± 0.045	0.354TrpMet: 0.354 ± 0.016	0.462TrpAsn: 0.462 ± 0.019	0.701TrpPro: 0.701 ± 0.023	0.597TrpGln: 0.597 ± 0.023	1.038TrpArg: 1.038 ± 0.033	0.869TrpSer: 0.869 ± 0.028	0.882TrpThr: 0.882 ± 0.026	1.09TrpVal: 1.09 ± 0.028	0.322TrpTrp: 0.322 ± 0.016	0.269TrpTyr: 0.269 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.272TyrAla: 2.272 ± 0.039	0.142TyrCys: 0.142 ± 0.012	1.231TyrAsp: 1.231 ± 0.032	1.011TyrGlu: 1.011 ± 0.027	0.754TyrPhe: 0.754 ± 0.025	1.894TyrGly: 1.894 ± 0.045	0.313TyrHis: 0.313 ± 0.016	0.717TyrIle: 0.717 ± 0.024	0.372TyrLys: 0.372 ± 0.017	2.281TyrLeu: 2.281 ± 0.042	0.277TyrMet: 0.277 ± 0.014	0.519TyrAsn: 0.519 ± 0.021	1.076TyrPro: 1.076 ± 0.027	0.616TyrGln: 0.616 ± 0.021	1.582TyrArg: 1.582 ± 0.037	1.288TyrSer: 1.288 ± 0.031	1.198TyrThr: 1.198 ± 0.034	1.552TyrVal: 1.552 ± 0.031	0.323TyrTrp: 0.323 ± 0.015	0.457TyrTyr: 0.457 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4157 proteins (1328963 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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