Aeromonas phage CC2
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 427 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6WBN7|I6WBN7_9CAUD Uncharacterized protein OS=Aeromonas phage CC2 OX=1204516 GN=CC2_028 PE=4 SV=1
MM1 pKa = 7.29 NRR3 pKa = 11.84 IDD5 pKa = 3.77 VEE7 pKa = 4.18 LEE9 pKa = 3.62 ARR11 pKa = 11.84 GVEE14 pKa = 3.77 ISGGYY19 pKa = 9.18 GHH21 pKa = 7.62 DD22 pKa = 3.45 PYY24 pKa = 10.87 IYY26 pKa = 9.68 LTLDD30 pKa = 3.13 QVDD33 pKa = 4.82 PEE35 pKa = 4.29 PVMNSISISEE45 pKa = 4.73 FISHH49 pKa = 7.41 FGDD52 pKa = 3.55 SDD54 pKa = 3.75 VLSYY58 pKa = 7.99 FTIQDD63 pKa = 3.29 ILAHH67 pKa = 6.26 FGEE70 pKa = 4.75 SEE72 pKa = 4.2 LLAAMDD78 pKa = 4.0 TQTIMDD84 pKa = 4.51 ALL86 pKa = 4.05
Molecular weight: 9.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.808
IPC_protein 3.745
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.062
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|I6WBF5|I6WBF5_9CAUD Uncharacterized protein OS=Aeromonas phage CC2 OX=1204516 GN=CC2_233 PE=4 SV=1
MM1 pKa = 7.79 AKK3 pKa = 9.53 QKK5 pKa = 10.84 KK6 pKa = 8.93 AVQKK10 pKa = 10.79 VEE12 pKa = 4.18 EE13 pKa = 4.32 KK14 pKa = 10.8 KK15 pKa = 9.38 LTGRR19 pKa = 11.84 QGYY22 pKa = 8.4 KK23 pKa = 9.54 RR24 pKa = 11.84 KK25 pKa = 10.5 SNGRR29 pKa = 11.84 VDD31 pKa = 3.56 QFGDD35 pKa = 3.28 ALRR38 pKa = 11.84 ARR40 pKa = 11.84 VRR42 pKa = 11.84 RR43 pKa = 11.84 AMKK46 pKa = 10.74 AEE48 pKa = 3.8 AFASKK53 pKa = 10.25 SLSHH57 pKa = 6.03 GTRR60 pKa = 11.84 EE61 pKa = 4.13 EE62 pKa = 3.89 ATQASTFKK70 pKa = 10.92 FF71 pKa = 3.65
Molecular weight: 8.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.253
IPC2_protein 9.823
IPC_protein 10.394
Toseland 11.125
ProMoST 10.774
Dawson 11.169
Bjellqvist 10.833
Wikipedia 11.359
Rodwell 11.55
Grimsley 11.184
Solomon 11.33
Lehninger 11.301
Nozaki 11.096
DTASelect 10.833
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.096
Patrickios 11.286
IPC_peptide 11.345
IPC2_peptide 9.37
IPC2.peptide.svr19 8.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
427
0
427
72370
36
1329
169.5
19.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.979 ± 0.165
1.331 ± 0.064
6.91 ± 0.117
7.152 ± 0.16
4.676 ± 0.096
6.337 ± 0.143
2.03 ± 0.068
7.18 ± 0.112
7.647 ± 0.191
6.912 ± 0.104
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.268 ± 0.097
5.511 ± 0.131
3.174 ± 0.079
2.983 ± 0.064
4.295 ± 0.079
6.49 ± 0.136
5.653 ± 0.183
7.47 ± 0.101
1.412 ± 0.057
4.592 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here