Amino acid dipepetide frequency for Bacillus phage Stills

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.31AlaAla: 5.31 ± 0.565
0.705AlaCys: 0.705 ± 0.182
3.568AlaAsp: 3.568 ± 0.582
4.48AlaGlu: 4.48 ± 0.561
3.111AlaPhe: 3.111 ± 0.379
4.024AlaGly: 4.024 ± 0.504
0.996AlaHis: 0.996 ± 0.204
4.397AlaIle: 4.397 ± 0.486
6.057AlaLys: 6.057 ± 0.518
4.563AlaLeu: 4.563 ± 0.385
1.825AlaMet: 1.825 ± 0.383
3.568AlaAsn: 3.568 ± 0.358
1.452AlaPro: 1.452 ± 0.258
2.572AlaGln: 2.572 ± 0.492
1.908AlaArg: 1.908 ± 0.31
3.858AlaSer: 3.858 ± 0.474
4.605AlaThr: 4.605 ± 0.724
4.605AlaVal: 4.605 ± 0.456
0.664AlaTrp: 0.664 ± 0.175
2.738AlaTyr: 2.738 ± 0.311
0.0AlaXaa: 0.0 ± 0.0
Cys
0.498CysAla: 0.498 ± 0.161
0.041CysCys: 0.041 ± 0.042
0.705CysAsp: 0.705 ± 0.176
0.622CysGlu: 0.622 ± 0.185
0.29CysPhe: 0.29 ± 0.094
0.747CysGly: 0.747 ± 0.236
0.207CysHis: 0.207 ± 0.092
0.622CysIle: 0.622 ± 0.175
0.415CysLys: 0.415 ± 0.139
0.581CysLeu: 0.581 ± 0.183
0.124CysMet: 0.124 ± 0.074
0.539CysAsn: 0.539 ± 0.158
0.373CysPro: 0.373 ± 0.149
0.373CysGln: 0.373 ± 0.136
0.373CysArg: 0.373 ± 0.144
0.498CysSer: 0.498 ± 0.144
0.622CysThr: 0.622 ± 0.209
0.415CysVal: 0.415 ± 0.166
0.041CysTrp: 0.041 ± 0.039
0.249CysTyr: 0.249 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
3.526AspAla: 3.526 ± 0.43
0.747AspCys: 0.747 ± 0.169
4.314AspAsp: 4.314 ± 0.342
4.231AspGlu: 4.231 ± 0.422
2.945AspPhe: 2.945 ± 0.35
4.771AspGly: 4.771 ± 0.52
0.871AspHis: 0.871 ± 0.218
5.186AspIle: 5.186 ± 0.425
4.522AspLys: 4.522 ± 0.484
5.891AspLeu: 5.891 ± 0.521
1.95AspMet: 1.95 ± 0.249
3.236AspAsn: 3.236 ± 0.304
1.95AspPro: 1.95 ± 0.286
2.323AspGln: 2.323 ± 0.355
2.24AspArg: 2.24 ± 0.394
3.194AspSer: 3.194 ± 0.503
3.651AspThr: 3.651 ± 0.323
5.186AspVal: 5.186 ± 0.47
0.747AspTrp: 0.747 ± 0.171
3.111AspTyr: 3.111 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
4.522GluAla: 4.522 ± 0.437
0.664GluCys: 0.664 ± 0.202
3.568GluAsp: 3.568 ± 0.506
6.928GluGlu: 6.928 ± 0.729
3.153GluPhe: 3.153 ± 0.331
4.149GluGly: 4.149 ± 0.422
1.203GluHis: 1.203 ± 0.23
4.771GluIle: 4.771 ± 0.45
6.928GluLys: 6.928 ± 0.791
6.762GluLeu: 6.762 ± 0.539
2.904GluMet: 2.904 ± 0.443
4.397GluAsn: 4.397 ± 0.487
1.659GluPro: 1.659 ± 0.354
3.028GluGln: 3.028 ± 0.363
3.568GluArg: 3.568 ± 0.524
3.568GluSer: 3.568 ± 0.389
4.314GluThr: 4.314 ± 0.417
5.352GluVal: 5.352 ± 0.6
1.12GluTrp: 1.12 ± 0.216
3.028GluTyr: 3.028 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
2.531PheAla: 2.531 ± 0.38
0.415PheCys: 0.415 ± 0.133
3.277PheAsp: 3.277 ± 0.295
4.024PheGlu: 4.024 ± 0.398
1.742PhePhe: 1.742 ± 0.303
2.655PheGly: 2.655 ± 0.399
0.581PheHis: 0.581 ± 0.21
3.07PheIle: 3.07 ± 0.416
3.485PheLys: 3.485 ± 0.33
3.817PheLeu: 3.817 ± 0.534
1.079PheMet: 1.079 ± 0.219
3.941PheAsn: 3.941 ± 0.37
1.286PhePro: 1.286 ± 0.272
1.867PheGln: 1.867 ± 0.256
1.701PheArg: 1.701 ± 0.268
2.448PheSer: 2.448 ± 0.269
3.236PheThr: 3.236 ± 0.361
2.489PheVal: 2.489 ± 0.302
0.456PheTrp: 0.456 ± 0.166
1.908PheTyr: 1.908 ± 0.316
0.0PheXaa: 0.0 ± 0.0
Gly
3.526GlyAla: 3.526 ± 0.375
0.498GlyCys: 0.498 ± 0.161
3.858GlyAsp: 3.858 ± 0.332
4.024GlyGlu: 4.024 ± 0.407
3.236GlyPhe: 3.236 ± 0.524
4.439GlyGly: 4.439 ± 0.525
1.12GlyHis: 1.12 ± 0.205
4.646GlyIle: 4.646 ± 0.452
5.02GlyLys: 5.02 ± 0.335
5.435GlyLeu: 5.435 ± 0.499
1.535GlyMet: 1.535 ± 0.286
3.153GlyAsn: 3.153 ± 0.435
0.29GlyPro: 0.29 ± 0.127
3.153GlyGln: 3.153 ± 0.516
2.282GlyArg: 2.282 ± 0.277
3.9GlySer: 3.9 ± 0.419
4.066GlyThr: 4.066 ± 0.589
3.941GlyVal: 3.941 ± 0.339
0.83GlyTrp: 0.83 ± 0.156
3.526GlyTyr: 3.526 ± 0.334
0.0GlyXaa: 0.0 ± 0.0
His
0.954HisAla: 0.954 ± 0.153
0.083HisCys: 0.083 ± 0.062
1.41HisAsp: 1.41 ± 0.276
1.41HisGlu: 1.41 ± 0.262
1.245HisPhe: 1.245 ± 0.241
0.705HisGly: 0.705 ± 0.181
0.456HisHis: 0.456 ± 0.157
1.079HisIle: 1.079 ± 0.248
1.12HisLys: 1.12 ± 0.237
1.328HisLeu: 1.328 ± 0.25
0.249HisMet: 0.249 ± 0.119
0.747HisAsn: 0.747 ± 0.157
0.498HisPro: 0.498 ± 0.135
0.747HisGln: 0.747 ± 0.175
0.664HisArg: 0.664 ± 0.197
0.747HisSer: 0.747 ± 0.191
0.954HisThr: 0.954 ± 0.186
1.037HisVal: 1.037 ± 0.208
0.29HisTrp: 0.29 ± 0.117
0.996HisTyr: 0.996 ± 0.162
0.0HisXaa: 0.0 ± 0.0
Ile
4.812IleAla: 4.812 ± 0.393
0.581IleCys: 0.581 ± 0.18
5.518IleAsp: 5.518 ± 0.483
5.227IleGlu: 5.227 ± 0.552
2.614IlePhe: 2.614 ± 0.289
3.983IleGly: 3.983 ± 0.386
1.162IleHis: 1.162 ± 0.226
3.775IleIle: 3.775 ± 0.433
6.638IleLys: 6.638 ± 0.502
3.817IleLeu: 3.817 ± 0.416
1.535IleMet: 1.535 ± 0.277
3.858IleAsn: 3.858 ± 0.43
1.867IlePro: 1.867 ± 0.215
2.697IleGln: 2.697 ± 0.332
2.904IleArg: 2.904 ± 0.368
3.983IleSer: 3.983 ± 0.532
4.19IleThr: 4.19 ± 0.47
4.605IleVal: 4.605 ± 0.497
0.373IleTrp: 0.373 ± 0.13
2.074IleTyr: 2.074 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
6.098LysAla: 6.098 ± 0.786
0.415LysCys: 0.415 ± 0.144
5.103LysAsp: 5.103 ± 0.46
7.301LysGlu: 7.301 ± 0.754
2.614LysPhe: 2.614 ± 0.264
4.978LysGly: 4.978 ± 0.411
1.576LysHis: 1.576 ± 0.311
5.476LysIle: 5.476 ± 0.633
7.509LysLys: 7.509 ± 0.551
6.928LysLeu: 6.928 ± 0.601
2.738LysMet: 2.738 ± 0.379
4.812LysAsn: 4.812 ± 0.483
3.111LysPro: 3.111 ± 0.489
3.277LysGln: 3.277 ± 0.471
4.273LysArg: 4.273 ± 0.476
4.522LysSer: 4.522 ± 0.447
5.435LysThr: 5.435 ± 0.54
5.683LysVal: 5.683 ± 0.512
0.83LysTrp: 0.83 ± 0.173
3.651LysTyr: 3.651 ± 0.362
0.0LysXaa: 0.0 ± 0.0
Leu
5.766LeuAla: 5.766 ± 0.473
0.664LeuCys: 0.664 ± 0.172
5.683LeuAsp: 5.683 ± 0.449
7.052LeuGlu: 7.052 ± 0.71
3.277LeuPhe: 3.277 ± 0.355
4.439LeuGly: 4.439 ± 0.342
1.41LeuHis: 1.41 ± 0.229
4.149LeuIle: 4.149 ± 0.482
7.55LeuLys: 7.55 ± 0.713
5.269LeuLeu: 5.269 ± 0.457
2.282LeuMet: 2.282 ± 0.317
4.605LeuAsn: 4.605 ± 0.426
2.033LeuPro: 2.033 ± 0.285
2.655LeuGln: 2.655 ± 0.373
2.862LeuArg: 2.862 ± 0.373
4.812LeuSer: 4.812 ± 0.397
4.729LeuThr: 4.729 ± 0.44
4.397LeuVal: 4.397 ± 0.44
0.913LeuTrp: 0.913 ± 0.237
2.572LeuTyr: 2.572 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
1.742MetAla: 1.742 ± 0.199
0.166MetCys: 0.166 ± 0.096
1.659MetAsp: 1.659 ± 0.272
2.78MetGlu: 2.78 ± 0.41
1.286MetPhe: 1.286 ± 0.176
1.867MetGly: 1.867 ± 0.41
0.29MetHis: 0.29 ± 0.117
2.323MetIle: 2.323 ± 0.332
2.531MetLys: 2.531 ± 0.421
1.784MetLeu: 1.784 ± 0.23
1.12MetMet: 1.12 ± 0.216
1.95MetAsn: 1.95 ± 0.253
0.83MetPro: 0.83 ± 0.238
1.452MetGln: 1.452 ± 0.261
0.913MetArg: 0.913 ± 0.188
1.95MetSer: 1.95 ± 0.266
1.95MetThr: 1.95 ± 0.333
1.784MetVal: 1.784 ± 0.26
0.373MetTrp: 0.373 ± 0.135
0.622MetTyr: 0.622 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
3.36AsnAla: 3.36 ± 0.616
0.332AsnCys: 0.332 ± 0.113
4.273AsnAsp: 4.273 ± 0.465
4.19AsnGlu: 4.19 ± 0.497
2.199AsnPhe: 2.199 ± 0.306
4.397AsnGly: 4.397 ± 0.455
1.037AsnHis: 1.037 ± 0.219
3.319AsnIle: 3.319 ± 0.354
5.476AsnLys: 5.476 ± 0.544
4.439AsnLeu: 4.439 ± 0.445
1.245AsnMet: 1.245 ± 0.247
3.07AsnAsn: 3.07 ± 0.354
2.448AsnPro: 2.448 ± 0.348
1.991AsnGln: 1.991 ± 0.359
2.365AsnArg: 2.365 ± 0.315
3.111AsnSer: 3.111 ± 0.43
3.609AsnThr: 3.609 ± 0.528
4.024AsnVal: 4.024 ± 0.489
0.622AsnTrp: 0.622 ± 0.189
1.784AsnTyr: 1.784 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
1.493ProAla: 1.493 ± 0.277
0.249ProCys: 0.249 ± 0.102
1.576ProAsp: 1.576 ± 0.343
1.867ProGlu: 1.867 ± 0.339
1.784ProPhe: 1.784 ± 0.265
0.996ProGly: 0.996 ± 0.164
0.498ProHis: 0.498 ± 0.157
1.867ProIle: 1.867 ± 0.332
2.655ProLys: 2.655 ± 0.391
1.867ProLeu: 1.867 ± 0.292
0.954ProMet: 0.954 ± 0.193
1.742ProAsn: 1.742 ± 0.27
0.498ProPro: 0.498 ± 0.182
1.037ProGln: 1.037 ± 0.178
0.788ProArg: 0.788 ± 0.151
2.199ProSer: 2.199 ± 0.277
2.157ProThr: 2.157 ± 0.353
1.618ProVal: 1.618 ± 0.279
0.166ProTrp: 0.166 ± 0.076
0.996ProTyr: 0.996 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
3.111GlnAla: 3.111 ± 0.581
0.29GlnCys: 0.29 ± 0.118
1.701GlnAsp: 1.701 ± 0.28
2.614GlnGlu: 2.614 ± 0.377
2.24GlnPhe: 2.24 ± 0.409
2.406GlnGly: 2.406 ± 0.35
0.373GlnHis: 0.373 ± 0.1
2.406GlnIle: 2.406 ± 0.409
3.443GlnLys: 3.443 ± 0.365
4.024GlnLeu: 4.024 ± 0.478
1.742GlnMet: 1.742 ± 0.367
1.867GlnAsn: 1.867 ± 0.334
0.996GlnPro: 0.996 ± 0.323
2.738GlnGln: 2.738 ± 0.739
1.784GlnArg: 1.784 ± 0.275
2.24GlnSer: 2.24 ± 0.383
1.991GlnThr: 1.991 ± 0.32
2.614GlnVal: 2.614 ± 0.337
0.373GlnTrp: 0.373 ± 0.103
1.12GlnTyr: 1.12 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
1.867ArgAla: 1.867 ± 0.302
0.29ArgCys: 0.29 ± 0.104
2.365ArgAsp: 2.365 ± 0.359
2.365ArgGlu: 2.365 ± 0.297
2.365ArgPhe: 2.365 ± 0.357
2.531ArgGly: 2.531 ± 0.358
0.581ArgHis: 0.581 ± 0.148
3.111ArgIle: 3.111 ± 0.313
4.688ArgLys: 4.688 ± 0.542
2.655ArgLeu: 2.655 ± 0.392
1.328ArgMet: 1.328 ± 0.26
1.825ArgAsn: 1.825 ± 0.315
1.203ArgPro: 1.203 ± 0.249
1.079ArgGln: 1.079 ± 0.243
1.286ArgArg: 1.286 ± 0.243
2.074ArgSer: 2.074 ± 0.302
2.738ArgThr: 2.738 ± 0.394
2.738ArgVal: 2.738 ± 0.319
0.581ArgTrp: 0.581 ± 0.153
1.493ArgTyr: 1.493 ± 0.269
0.0ArgXaa: 0.0 ± 0.0
Ser
3.817SerAla: 3.817 ± 0.49
0.622SerCys: 0.622 ± 0.232
3.858SerAsp: 3.858 ± 0.38
3.651SerGlu: 3.651 ± 0.458
2.821SerPhe: 2.821 ± 0.406
4.314SerGly: 4.314 ± 0.612
1.037SerHis: 1.037 ± 0.242
4.024SerIle: 4.024 ± 0.374
3.609SerLys: 3.609 ± 0.445
3.941SerLeu: 3.941 ± 0.581
1.95SerMet: 1.95 ± 0.265
3.028SerAsn: 3.028 ± 0.379
1.41SerPro: 1.41 ± 0.269
2.489SerGln: 2.489 ± 0.464
2.365SerArg: 2.365 ± 0.341
4.273SerSer: 4.273 ± 0.523
3.153SerThr: 3.153 ± 0.393
3.817SerVal: 3.817 ± 0.418
0.788SerTrp: 0.788 ± 0.151
2.655SerTyr: 2.655 ± 0.342
0.0SerXaa: 0.0 ± 0.0
Thr
5.02ThrAla: 5.02 ± 0.911
0.415ThrCys: 0.415 ± 0.142
3.775ThrAsp: 3.775 ± 0.431
4.439ThrGlu: 4.439 ± 0.423
3.858ThrPhe: 3.858 ± 0.376
4.522ThrGly: 4.522 ± 0.625
0.871ThrHis: 0.871 ± 0.2
3.858ThrIle: 3.858 ± 0.44
4.522ThrLys: 4.522 ± 0.453
4.937ThrLeu: 4.937 ± 0.435
1.452ThrMet: 1.452 ± 0.25
3.526ThrAsn: 3.526 ± 0.388
2.365ThrPro: 2.365 ± 0.286
2.24ThrGln: 2.24 ± 0.301
1.784ThrArg: 1.784 ± 0.3
3.651ThrSer: 3.651 ± 0.432
4.397ThrThr: 4.397 ± 0.636
4.231ThrVal: 4.231 ± 0.328
0.622ThrTrp: 0.622 ± 0.118
2.862ThrTyr: 2.862 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
3.319ValAla: 3.319 ± 0.411
0.498ValCys: 0.498 ± 0.172
4.273ValAsp: 4.273 ± 0.484
4.978ValGlu: 4.978 ± 0.507
2.821ValPhe: 2.821 ± 0.332
3.734ValGly: 3.734 ± 0.486
1.245ValHis: 1.245 ± 0.213
4.937ValIle: 4.937 ± 0.441
6.098ValLys: 6.098 ± 0.451
5.061ValLeu: 5.061 ± 0.387
1.784ValMet: 1.784 ± 0.29
4.397ValAsn: 4.397 ± 0.36
1.825ValPro: 1.825 ± 0.257
2.945ValGln: 2.945 ± 0.399
2.904ValArg: 2.904 ± 0.31
3.36ValSer: 3.36 ± 0.402
4.314ValThr: 4.314 ± 0.449
3.983ValVal: 3.983 ± 0.402
0.747ValTrp: 0.747 ± 0.178
2.697ValTyr: 2.697 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
0.456TrpAla: 0.456 ± 0.139
0.207TrpCys: 0.207 ± 0.103
0.705TrpAsp: 0.705 ± 0.181
0.664TrpGlu: 0.664 ± 0.168
0.539TrpPhe: 0.539 ± 0.162
0.664TrpGly: 0.664 ± 0.169
0.29TrpHis: 0.29 ± 0.127
0.954TrpIle: 0.954 ± 0.182
0.913TrpLys: 0.913 ± 0.214
0.954TrpLeu: 0.954 ± 0.232
0.332TrpMet: 0.332 ± 0.118
0.332TrpAsn: 0.332 ± 0.105
0.124TrpPro: 0.124 ± 0.08
0.332TrpGln: 0.332 ± 0.125
0.622TrpArg: 0.622 ± 0.171
0.954TrpSer: 0.954 ± 0.215
0.747TrpThr: 0.747 ± 0.18
0.581TrpVal: 0.581 ± 0.124
0.124TrpTrp: 0.124 ± 0.096
0.664TrpTyr: 0.664 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.277TyrAla: 3.277 ± 0.392
0.456TyrCys: 0.456 ± 0.141
3.402TyrAsp: 3.402 ± 0.326
2.738TyrGlu: 2.738 ± 0.409
1.867TyrPhe: 1.867 ± 0.288
2.157TyrGly: 2.157 ± 0.259
0.913TyrHis: 0.913 ± 0.243
2.24TyrIle: 2.24 ± 0.42
3.153TyrLys: 3.153 ± 0.427
3.111TyrLeu: 3.111 ± 0.399
1.203TyrMet: 1.203 ± 0.236
2.697TyrAsn: 2.697 ± 0.367
0.788TyrPro: 0.788 ± 0.205
1.079TyrGln: 1.079 ± 0.178
1.576TyrArg: 1.576 ± 0.312
2.282TyrSer: 2.282 ± 0.32
2.489TyrThr: 2.489 ± 0.284
2.821TyrVal: 2.821 ± 0.465
0.498TyrTrp: 0.498 ± 0.154
1.908TyrTyr: 1.908 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 110 proteins (24106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski