Lachnospiraceae bacterium G41

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2367 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8RD74|A0A1G8RD74_9FIRM Alpha-glucosidase OS=Lachnospiraceae bacterium G41 OX=1200749 GN=SAMN04487760_10339 PE=4 SV=1
MM1 pKa = 7.26EE2 pKa = 5.76RR3 pKa = 11.84KK4 pKa = 9.48DD5 pKa = 4.07ANFFSAQIRR14 pKa = 11.84LWAGIFDD21 pKa = 3.91YY22 pKa = 10.67KK23 pKa = 10.91GRR25 pKa = 11.84STRR28 pKa = 11.84KK29 pKa = 8.47EE30 pKa = 3.37YY31 pKa = 9.91WFAFLFHH38 pKa = 6.96FGLGVLAFFGMLISSAIFLLQDD60 pKa = 3.65FVDD63 pKa = 5.37ASILLNIVHH72 pKa = 7.51FILLIPAVLILIYY85 pKa = 10.27LGLSIIPWIALTVRR99 pKa = 11.84RR100 pKa = 11.84LRR102 pKa = 11.84DD103 pKa = 3.36AGKK106 pKa = 10.36SGWWTILLLFVGIGHH121 pKa = 7.71IILLILCALGSAVVGVAFFAANNIQPSVYY150 pKa = 10.27GPPEE154 pKa = 3.84WFDD157 pKa = 3.63PDD159 pKa = 4.18VNINDD164 pKa = 4.11GVYY167 pKa = 10.5GPPGPDD173 pKa = 3.12IFNPDD178 pKa = 3.83DD179 pKa = 3.94NEE181 pKa = 4.3TPTVYY186 pKa = 10.54GPPEE190 pKa = 4.25WFDD193 pKa = 4.95DD194 pKa = 5.13DD195 pKa = 6.26DD196 pKa = 6.67DD197 pKa = 5.29FDD199 pKa = 5.24PSINDD204 pKa = 3.43SEE206 pKa = 4.44PLYY209 pKa = 10.76GPPPFDD215 pKa = 4.63EE216 pKa = 6.15DD217 pKa = 5.8DD218 pKa = 3.71YY219 pKa = 11.98DD220 pKa = 4.46PNINQNEE227 pKa = 4.28DD228 pKa = 3.72VYY230 pKa = 11.5GPPEE234 pKa = 4.14WLNDD238 pKa = 3.52PDD240 pKa = 4.59EE241 pKa = 4.81YY242 pKa = 11.49DD243 pKa = 3.61PEE245 pKa = 4.48VNLNEE250 pKa = 4.49DD251 pKa = 3.89VYY253 pKa = 11.42GPPEE257 pKa = 3.65WFEE260 pKa = 4.17EE261 pKa = 4.22NEE263 pKa = 3.77NDD265 pKa = 4.87FDD267 pKa = 4.39VDD269 pKa = 4.41LNMEE273 pKa = 4.2PTVYY277 pKa = 10.56GPPDD281 pKa = 3.1WFEE284 pKa = 3.71

Molecular weight: 32.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8U294|A0A1G8U294_9FIRM tRNA (guanine-N(7)-)-methyltransferase OS=Lachnospiraceae bacterium G41 OX=1200749 GN=trmB PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.93KK9 pKa = 7.56RR10 pKa = 11.84QRR12 pKa = 11.84NKK14 pKa = 8.21VHH16 pKa = 6.58GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.72VLQARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.62GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2367

0

2367

793647

39

3850

335.3

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.754 ± 0.05

1.439 ± 0.022

6.338 ± 0.047

7.93 ± 0.064

4.713 ± 0.045

6.677 ± 0.043

1.461 ± 0.018

8.224 ± 0.053

7.91 ± 0.049

8.502 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.026

5.177 ± 0.044

3.118 ± 0.028

2.126 ± 0.026

3.554 ± 0.034

5.947 ± 0.044

5.205 ± 0.051

6.812 ± 0.038

0.831 ± 0.018

4.517 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski