Oyster mushroom spherical virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 8.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q80QV2|Q80QV2_9VIRU RNA-dependent RNA polymerase OS=Oyster mushroom spherical virus OX=218667 PE=4 SV=1
MM1 pKa = 7.35VGADD5 pKa = 4.29LFAQISSSNHH15 pKa = 5.24SDD17 pKa = 3.62NIIGEE22 pKa = 3.95ALGRR26 pKa = 11.84YY27 pKa = 7.82RR28 pKa = 11.84AHH30 pKa = 7.43EE31 pKa = 4.1SRR33 pKa = 11.84AVQVISSVLTPSEE46 pKa = 3.89KK47 pKa = 10.76ALADD51 pKa = 3.58RR52 pKa = 11.84WAIPYY57 pKa = 10.03SSSPVGRR64 pKa = 11.84VPHH67 pKa = 6.06VMLKK71 pKa = 10.62ALEE74 pKa = 3.85NHH76 pKa = 6.4RR77 pKa = 11.84NVNILPNYY85 pKa = 9.04LRR87 pKa = 11.84GRR89 pKa = 11.84VTVLSMKK96 pKa = 9.3PAKK99 pKa = 10.05LAALRR104 pKa = 11.84ARR106 pKa = 11.84CPGVQFNLVNEE117 pKa = 5.08IITPADD123 pKa = 3.15HH124 pKa = 6.85TRR126 pKa = 11.84YY127 pKa = 8.22TDD129 pKa = 3.77PSPPPDD135 pKa = 3.64FQGPGDD141 pKa = 4.38MILVDD146 pKa = 5.29DD147 pKa = 5.73ALQHH151 pKa = 5.82WSRR154 pKa = 11.84EE155 pKa = 4.17RR156 pKa = 11.84IDD158 pKa = 3.11AAFRR162 pKa = 11.84HH163 pKa = 5.91FGGNRR168 pKa = 11.84LIGTNIHH175 pKa = 6.57PDD177 pKa = 3.41EE178 pKa = 4.55VRR180 pKa = 11.84SGHH183 pKa = 6.37ASRR186 pKa = 11.84YY187 pKa = 7.27PDD189 pKa = 4.3LYY191 pKa = 10.4TIEE194 pKa = 4.2YY195 pKa = 9.46LPNRR199 pKa = 11.84RR200 pKa = 11.84YY201 pKa = 10.33GLLSHH206 pKa = 6.22RR207 pKa = 11.84HH208 pKa = 5.2KK209 pKa = 10.57SASYY213 pKa = 9.14EE214 pKa = 3.94ASIDD218 pKa = 3.94EE219 pKa = 5.04AWMLDD224 pKa = 3.63CGDD227 pKa = 3.77FTVDD231 pKa = 3.0DD232 pKa = 3.67RR233 pKa = 11.84AYY235 pKa = 10.55DD236 pKa = 3.74VEE238 pKa = 4.64FLLSYY243 pKa = 10.79GPYY246 pKa = 9.77HH247 pKa = 7.04LVTVVPADD255 pKa = 3.35GHH257 pKa = 4.51VVRR260 pKa = 11.84TYY262 pKa = 11.18RR263 pKa = 11.84FFDD266 pKa = 3.55APEE269 pKa = 4.15VVQLPDD275 pKa = 3.76IPGLPNLSSPWFPALMYY292 pKa = 9.03TQTLDD297 pKa = 3.14HH298 pKa = 6.88AGSLKK303 pKa = 10.4QLGEE307 pKa = 4.1RR308 pKa = 11.84DD309 pKa = 3.09GKK311 pKa = 10.96ARR313 pKa = 11.84IRR315 pKa = 11.84GLRR318 pKa = 11.84MTPEE322 pKa = 4.21GRR324 pKa = 11.84RR325 pKa = 11.84IPYY328 pKa = 7.77ATWEE332 pKa = 4.09RR333 pKa = 11.84LLTCAKK339 pKa = 10.39LAGLHH344 pKa = 5.9VDD346 pKa = 3.49SEE348 pKa = 4.46EE349 pKa = 4.58LEE351 pKa = 5.11GITSVGLSRR360 pKa = 11.84LRR362 pKa = 11.84FFIRR366 pKa = 11.84RR367 pKa = 11.84KK368 pKa = 9.84AEE370 pKa = 3.35EE371 pKa = 4.51WLPDD375 pKa = 3.28WLFEE379 pKa = 4.33LLYY382 pKa = 10.27RR383 pKa = 11.84DD384 pKa = 3.57FVIARR389 pKa = 11.84RR390 pKa = 11.84VRR392 pKa = 11.84RR393 pKa = 11.84ALTQDD398 pKa = 2.55IVVIKK403 pKa = 10.67VPLRR407 pKa = 11.84SLPSIPSQPWVSSVPAVPVVAPTPPPPPPPAPPFGGGDD445 pKa = 3.29SGGGGGNDD453 pKa = 3.32RR454 pKa = 11.84PPRR457 pKa = 11.84PPAGPSQSNGSKK469 pKa = 9.99NVQSKK474 pKa = 10.76SVGASAQRR482 pKa = 11.84KK483 pKa = 8.48LNARR487 pKa = 11.84AAPPLNRR494 pKa = 11.84AHH496 pKa = 7.35PVRR499 pKa = 11.84PNANPVPRR507 pKa = 11.84VAASSVRR514 pKa = 11.84ANFLGVLAAQRR525 pKa = 11.84AAPTVSWKK533 pKa = 10.45SAEE536 pKa = 4.15SNMAVDD542 pKa = 4.47PNSCYY547 pKa = 10.58VRR549 pKa = 11.84VAHH552 pKa = 6.95EE553 pKa = 3.94ILGVSDD559 pKa = 3.44QANEE563 pKa = 4.0IIRR566 pKa = 11.84RR567 pKa = 11.84TIASLPVDD575 pKa = 4.97EE576 pKa = 5.41IALLRR581 pKa = 11.84PGGAGATQRR590 pKa = 11.84FFHH593 pKa = 6.81ALGLLADD600 pKa = 4.07LRR602 pKa = 11.84FDD604 pKa = 3.72IVFHH608 pKa = 6.56SPVPNDD614 pKa = 3.03TLAQVGRR621 pKa = 11.84LDD623 pKa = 3.95GTVHH627 pKa = 6.01TLHH630 pKa = 6.87WSVQGTQGHH639 pKa = 6.09WSRR642 pKa = 11.84AVPNTPATTGSTGSTTGNGPFVSGNALPYY671 pKa = 10.3QRR673 pKa = 11.84FLGSVRR679 pKa = 11.84QMGWQSFQFTPSLTFAKK696 pKa = 10.11RR697 pKa = 11.84LYY699 pKa = 10.72NEE701 pKa = 4.61LEE703 pKa = 3.94VEE705 pKa = 4.14ITGKK709 pKa = 7.84ITKK712 pKa = 8.78IDD714 pKa = 3.36KK715 pKa = 8.96WASCHH720 pKa = 6.62KK721 pKa = 9.34NIEE724 pKa = 3.84MAARR728 pKa = 11.84NGCVRR733 pKa = 11.84PRR735 pKa = 11.84EE736 pKa = 4.16VIYY739 pKa = 10.64LAGTAGCGKK748 pKa = 10.2SSGVADD754 pKa = 3.68LVQRR758 pKa = 11.84HH759 pKa = 5.77GNLGYY764 pKa = 9.17WLSVVSPLNDD774 pKa = 3.77LNEE777 pKa = 3.97EE778 pKa = 4.0WTEE781 pKa = 4.16KK782 pKa = 9.38ITGLSSEE789 pKa = 3.82QRR791 pKa = 11.84KK792 pKa = 8.99IFKK795 pKa = 8.54THH797 pKa = 5.36EE798 pKa = 4.16KK799 pKa = 11.19AMFAQPQVVVFDD811 pKa = 4.28EE812 pKa = 4.35AQKK815 pKa = 11.28LPGHH819 pKa = 5.87YY820 pKa = 10.02LDD822 pKa = 5.93LYY824 pKa = 10.36LAYY827 pKa = 10.2HH828 pKa = 7.23PDD830 pKa = 3.46VEE832 pKa = 4.9LVILLGDD839 pKa = 4.44PFQSGAPVTDD849 pKa = 3.35QRR851 pKa = 11.84SLLFPEE857 pKa = 4.71MSPGRR862 pKa = 11.84TLARR866 pKa = 11.84HH867 pKa = 4.55ATFYY871 pKa = 10.61LTDD874 pKa = 3.15SWRR877 pKa = 11.84VNPTVGQAWGIPVLHH892 pKa = 5.88STRR895 pKa = 11.84SNVYY899 pKa = 8.67WIRR902 pKa = 11.84GINPDD907 pKa = 3.28MPVIVSTVTAQNARR921 pKa = 11.84RR922 pKa = 11.84QYY924 pKa = 11.02GEE926 pKa = 3.58QAFTFSSCGGLDD938 pKa = 3.3FPGYY942 pKa = 8.38YY943 pKa = 9.91QIVLTRR949 pKa = 11.84EE950 pKa = 4.09LLSAVPDD957 pKa = 4.02DD958 pKa = 5.22AIYY961 pKa = 8.98TAFTRR966 pKa = 11.84SRR968 pKa = 11.84HH969 pKa = 6.24DD970 pKa = 3.18IAVLNALSPQEE981 pKa = 5.1FRR983 pKa = 11.84TACNNSRR990 pKa = 11.84LLNALFNGVPLNGTYY1005 pKa = 10.14RR1006 pKa = 11.84DD1007 pKa = 4.0VIGDD1011 pKa = 4.74RR1012 pKa = 11.84IPGVPLLGGFSLVHH1026 pKa = 5.55GTRR1029 pKa = 11.84LTTVADD1035 pKa = 3.51VLGNDD1040 pKa = 3.38GSKK1043 pKa = 10.84YY1044 pKa = 10.97DD1045 pKa = 3.79NLEE1048 pKa = 4.68PIMKK1052 pKa = 9.95ASMLAPPPEE1061 pKa = 4.17RR1062 pKa = 11.84VEE1064 pKa = 4.62SDD1066 pKa = 3.57VSPVPLKK1073 pKa = 10.44HH1074 pKa = 6.79APPNDD1079 pKa = 3.23EE1080 pKa = 3.8WVPTYY1085 pKa = 10.5FGRR1088 pKa = 11.84APHH1091 pKa = 6.87LKK1093 pKa = 10.11NDD1095 pKa = 3.72LNNTGFVRR1103 pKa = 11.84EE1104 pKa = 4.06NVEE1107 pKa = 3.95FLSPDD1112 pKa = 3.13GQMGRR1117 pKa = 11.84MFGSDD1122 pKa = 4.01RR1123 pKa = 11.84KK1124 pKa = 10.34PEE1126 pKa = 4.19DD1127 pKa = 3.23ASRR1130 pKa = 11.84VDD1132 pKa = 3.32GLNDD1136 pKa = 4.0FFPLHH1141 pKa = 6.06QSSDD1145 pKa = 3.49PALWGPTVPKK1155 pKa = 10.17RR1156 pKa = 11.84LRR1158 pKa = 11.84FGTEE1162 pKa = 3.09QGNRR1166 pKa = 11.84KK1167 pKa = 8.64EE1168 pKa = 4.22LADD1171 pKa = 3.98SSFLGPMLCDD1181 pKa = 3.91AFRR1184 pKa = 11.84KK1185 pKa = 9.2SHH1187 pKa = 6.36NLPFDD1192 pKa = 3.84IPWDD1196 pKa = 3.42QEE1198 pKa = 4.0LFDD1201 pKa = 5.09RR1202 pKa = 11.84CVEE1205 pKa = 4.06DD1206 pKa = 3.95CAQKK1210 pKa = 10.68RR1211 pKa = 11.84LSKK1214 pKa = 10.67SAEE1217 pKa = 3.73ALNNLTRR1224 pKa = 11.84DD1225 pKa = 3.13QDD1227 pKa = 4.32PILRR1231 pKa = 11.84DD1232 pKa = 3.38NKK1234 pKa = 10.57KK1235 pKa = 10.14VLNFLKK1241 pKa = 10.72GQLINKK1247 pKa = 9.24LDD1249 pKa = 5.0AITKK1253 pKa = 9.82GSGAEE1258 pKa = 3.95SFPTIKK1264 pKa = 9.47PAQMITTYY1272 pKa = 9.91TEE1274 pKa = 3.72EE1275 pKa = 3.91VNAAFGPYY1283 pKa = 9.02IRR1285 pKa = 11.84YY1286 pKa = 8.28LACKK1290 pKa = 9.76LRR1292 pKa = 11.84EE1293 pKa = 4.05HH1294 pKa = 6.83SPDD1297 pKa = 3.08HH1298 pKa = 5.96VMHH1301 pKa = 6.43YY1302 pKa = 11.16GGMDD1306 pKa = 4.04LNDD1309 pKa = 3.72LGQWARR1315 pKa = 11.84EE1316 pKa = 3.98HH1317 pKa = 6.36VPSKK1321 pKa = 9.0RR1322 pKa = 11.84TKK1324 pKa = 10.78SFTNDD1329 pKa = 2.63YY1330 pKa = 8.78TAYY1333 pKa = 10.29DD1334 pKa = 3.68KK1335 pKa = 11.18SCRR1338 pKa = 11.84GEE1340 pKa = 3.87TLAFEE1345 pKa = 4.26ICIMRR1350 pKa = 11.84LFNIPEE1356 pKa = 4.36WIIEE1360 pKa = 4.0LHH1362 pKa = 6.52VEE1364 pKa = 4.04LTLCVTSALGKK1375 pKa = 9.92LGIMRR1380 pKa = 11.84TSGQWCTYY1388 pKa = 10.52LFNTWYY1394 pKa = 10.47NDD1396 pKa = 2.97AYY1398 pKa = 9.97FTLKK1402 pKa = 10.7YY1403 pKa = 10.25VFPLNTPRR1411 pKa = 11.84GFSGDD1416 pKa = 3.94DD1417 pKa = 3.33MFILCVPTISPGWLSISRR1435 pKa = 11.84YY1436 pKa = 8.69FSLVGKK1442 pKa = 8.68PVITFLPEE1450 pKa = 3.51FCGWLLTSHH1459 pKa = 7.07GIIRR1463 pKa = 11.84HH1464 pKa = 6.02PYY1466 pKa = 8.91LLYY1469 pKa = 10.87AKK1471 pKa = 10.39YY1472 pKa = 10.45LFHH1475 pKa = 7.6KK1476 pKa = 10.67EE1477 pKa = 3.65NGTLANVLISYY1488 pKa = 10.31YY1489 pKa = 9.69MEE1491 pKa = 4.38FQHH1494 pKa = 7.19SLTLGDD1500 pKa = 5.12ALWEE1504 pKa = 4.1SLPYY1508 pKa = 10.63DD1509 pKa = 4.03LLPYY1513 pKa = 10.12QGQLQLLFNQARR1525 pKa = 11.84SRR1527 pKa = 11.84VPSFLIRR1534 pKa = 11.84EE1535 pKa = 4.33NKK1537 pKa = 10.52LGDD1540 pKa = 3.49APAAKK1545 pKa = 10.41LSARR1549 pKa = 11.84YY1550 pKa = 9.73DD1551 pKa = 3.89LFRR1554 pKa = 11.84LPADD1558 pKa = 4.26FMRR1561 pKa = 11.84MDD1563 pKa = 3.32WRR1565 pKa = 11.84LLPSSIRR1572 pKa = 11.84RR1573 pKa = 11.84SLLLARR1579 pKa = 11.84GLL1581 pKa = 3.77

Molecular weight:
176.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q80QV2|Q80QV2_9VIRU RNA-dependent RNA polymerase OS=Oyster mushroom spherical virus OX=218667 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84FVLGTLHH9 pKa = 6.94VIPISTPLSTFRR21 pKa = 11.84TGAMVFCPTDD31 pKa = 3.55TSPLPMKK38 pKa = 9.66PLLMRR43 pKa = 11.84RR44 pKa = 11.84GCWTVAISPLMTVRR58 pKa = 11.84TMLNFYY64 pKa = 10.22CLTARR69 pKa = 11.84TILSLLFQPMAMWFALTGFSMPLRR93 pKa = 11.84WFSFQTSQVCPISPPPGSLLL113 pKa = 3.74

Molecular weight:
12.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2579

113

1581

368.4

40.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.755 ± 0.321

1.9 ± 0.634

4.071 ± 1.188

3.218 ± 0.891

5.118 ± 0.682

6.863 ± 1.212

2.21 ± 0.562

4.071 ± 0.276

2.675 ± 0.57

11.167 ± 1.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.552

3.645 ± 0.649

8.802 ± 1.008

2.831 ± 0.387

7.367 ± 0.393

9.267 ± 1.641

6.475 ± 1.201

6.359 ± 0.734

1.59 ± 0.252

2.404 ± 0.718

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski