Oyster mushroom spherical virus
Average proteome isoelectric point is 8.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q80QV2|Q80QV2_9VIRU RNA-dependent RNA polymerase OS=Oyster mushroom spherical virus OX=218667 PE=4 SV=1
MM1 pKa = 7.35 VGADD5 pKa = 4.29 LFAQISSSNHH15 pKa = 5.24 SDD17 pKa = 3.62 NIIGEE22 pKa = 3.95 ALGRR26 pKa = 11.84 YY27 pKa = 7.82 RR28 pKa = 11.84 AHH30 pKa = 7.43 EE31 pKa = 4.1 SRR33 pKa = 11.84 AVQVISSVLTPSEE46 pKa = 3.89 KK47 pKa = 10.76 ALADD51 pKa = 3.58 RR52 pKa = 11.84 WAIPYY57 pKa = 10.03 SSSPVGRR64 pKa = 11.84 VPHH67 pKa = 6.06 VMLKK71 pKa = 10.62 ALEE74 pKa = 3.85 NHH76 pKa = 6.4 RR77 pKa = 11.84 NVNILPNYY85 pKa = 9.04 LRR87 pKa = 11.84 GRR89 pKa = 11.84 VTVLSMKK96 pKa = 9.3 PAKK99 pKa = 10.05 LAALRR104 pKa = 11.84 ARR106 pKa = 11.84 CPGVQFNLVNEE117 pKa = 5.08 IITPADD123 pKa = 3.15 HH124 pKa = 6.85 TRR126 pKa = 11.84 YY127 pKa = 8.22 TDD129 pKa = 3.77 PSPPPDD135 pKa = 3.64 FQGPGDD141 pKa = 4.38 MILVDD146 pKa = 5.29 DD147 pKa = 5.73 ALQHH151 pKa = 5.82 WSRR154 pKa = 11.84 EE155 pKa = 4.17 RR156 pKa = 11.84 IDD158 pKa = 3.11 AAFRR162 pKa = 11.84 HH163 pKa = 5.91 FGGNRR168 pKa = 11.84 LIGTNIHH175 pKa = 6.57 PDD177 pKa = 3.41 EE178 pKa = 4.55 VRR180 pKa = 11.84 SGHH183 pKa = 6.37 ASRR186 pKa = 11.84 YY187 pKa = 7.27 PDD189 pKa = 4.3 LYY191 pKa = 10.4 TIEE194 pKa = 4.2 YY195 pKa = 9.46 LPNRR199 pKa = 11.84 RR200 pKa = 11.84 YY201 pKa = 10.33 GLLSHH206 pKa = 6.22 RR207 pKa = 11.84 HH208 pKa = 5.2 KK209 pKa = 10.57 SASYY213 pKa = 9.14 EE214 pKa = 3.94 ASIDD218 pKa = 3.94 EE219 pKa = 5.04 AWMLDD224 pKa = 3.63 CGDD227 pKa = 3.77 FTVDD231 pKa = 3.0 DD232 pKa = 3.67 RR233 pKa = 11.84 AYY235 pKa = 10.55 DD236 pKa = 3.74 VEE238 pKa = 4.64 FLLSYY243 pKa = 10.79 GPYY246 pKa = 9.77 HH247 pKa = 7.04 LVTVVPADD255 pKa = 3.35 GHH257 pKa = 4.51 VVRR260 pKa = 11.84 TYY262 pKa = 11.18 RR263 pKa = 11.84 FFDD266 pKa = 3.55 APEE269 pKa = 4.15 VVQLPDD275 pKa = 3.76 IPGLPNLSSPWFPALMYY292 pKa = 9.03 TQTLDD297 pKa = 3.14 HH298 pKa = 6.88 AGSLKK303 pKa = 10.4 QLGEE307 pKa = 4.1 RR308 pKa = 11.84 DD309 pKa = 3.09 GKK311 pKa = 10.96 ARR313 pKa = 11.84 IRR315 pKa = 11.84 GLRR318 pKa = 11.84 MTPEE322 pKa = 4.21 GRR324 pKa = 11.84 RR325 pKa = 11.84 IPYY328 pKa = 7.77 ATWEE332 pKa = 4.09 RR333 pKa = 11.84 LLTCAKK339 pKa = 10.39 LAGLHH344 pKa = 5.9 VDD346 pKa = 3.49 SEE348 pKa = 4.46 EE349 pKa = 4.58 LEE351 pKa = 5.11 GITSVGLSRR360 pKa = 11.84 LRR362 pKa = 11.84 FFIRR366 pKa = 11.84 RR367 pKa = 11.84 KK368 pKa = 9.84 AEE370 pKa = 3.35 EE371 pKa = 4.51 WLPDD375 pKa = 3.28 WLFEE379 pKa = 4.33 LLYY382 pKa = 10.27 RR383 pKa = 11.84 DD384 pKa = 3.57 FVIARR389 pKa = 11.84 RR390 pKa = 11.84 VRR392 pKa = 11.84 RR393 pKa = 11.84 ALTQDD398 pKa = 2.55 IVVIKK403 pKa = 10.67 VPLRR407 pKa = 11.84 SLPSIPSQPWVSSVPAVPVVAPTPPPPPPPAPPFGGGDD445 pKa = 3.29 SGGGGGNDD453 pKa = 3.32 RR454 pKa = 11.84 PPRR457 pKa = 11.84 PPAGPSQSNGSKK469 pKa = 9.99 NVQSKK474 pKa = 10.76 SVGASAQRR482 pKa = 11.84 KK483 pKa = 8.48 LNARR487 pKa = 11.84 AAPPLNRR494 pKa = 11.84 AHH496 pKa = 7.35 PVRR499 pKa = 11.84 PNANPVPRR507 pKa = 11.84 VAASSVRR514 pKa = 11.84 ANFLGVLAAQRR525 pKa = 11.84 AAPTVSWKK533 pKa = 10.45 SAEE536 pKa = 4.15 SNMAVDD542 pKa = 4.47 PNSCYY547 pKa = 10.58 VRR549 pKa = 11.84 VAHH552 pKa = 6.95 EE553 pKa = 3.94 ILGVSDD559 pKa = 3.44 QANEE563 pKa = 4.0 IIRR566 pKa = 11.84 RR567 pKa = 11.84 TIASLPVDD575 pKa = 4.97 EE576 pKa = 5.41 IALLRR581 pKa = 11.84 PGGAGATQRR590 pKa = 11.84 FFHH593 pKa = 6.81 ALGLLADD600 pKa = 4.07 LRR602 pKa = 11.84 FDD604 pKa = 3.72 IVFHH608 pKa = 6.56 SPVPNDD614 pKa = 3.03 TLAQVGRR621 pKa = 11.84 LDD623 pKa = 3.95 GTVHH627 pKa = 6.01 TLHH630 pKa = 6.87 WSVQGTQGHH639 pKa = 6.09 WSRR642 pKa = 11.84 AVPNTPATTGSTGSTTGNGPFVSGNALPYY671 pKa = 10.3 QRR673 pKa = 11.84 FLGSVRR679 pKa = 11.84 QMGWQSFQFTPSLTFAKK696 pKa = 10.11 RR697 pKa = 11.84 LYY699 pKa = 10.72 NEE701 pKa = 4.61 LEE703 pKa = 3.94 VEE705 pKa = 4.14 ITGKK709 pKa = 7.84 ITKK712 pKa = 8.78 IDD714 pKa = 3.36 KK715 pKa = 8.96 WASCHH720 pKa = 6.62 KK721 pKa = 9.34 NIEE724 pKa = 3.84 MAARR728 pKa = 11.84 NGCVRR733 pKa = 11.84 PRR735 pKa = 11.84 EE736 pKa = 4.16 VIYY739 pKa = 10.64 LAGTAGCGKK748 pKa = 10.2 SSGVADD754 pKa = 3.68 LVQRR758 pKa = 11.84 HH759 pKa = 5.77 GNLGYY764 pKa = 9.17 WLSVVSPLNDD774 pKa = 3.77 LNEE777 pKa = 3.97 EE778 pKa = 4.0 WTEE781 pKa = 4.16 KK782 pKa = 9.38 ITGLSSEE789 pKa = 3.82 QRR791 pKa = 11.84 KK792 pKa = 8.99 IFKK795 pKa = 8.54 THH797 pKa = 5.36 EE798 pKa = 4.16 KK799 pKa = 11.19 AMFAQPQVVVFDD811 pKa = 4.28 EE812 pKa = 4.35 AQKK815 pKa = 11.28 LPGHH819 pKa = 5.87 YY820 pKa = 10.02 LDD822 pKa = 5.93 LYY824 pKa = 10.36 LAYY827 pKa = 10.2 HH828 pKa = 7.23 PDD830 pKa = 3.46 VEE832 pKa = 4.9 LVILLGDD839 pKa = 4.44 PFQSGAPVTDD849 pKa = 3.35 QRR851 pKa = 11.84 SLLFPEE857 pKa = 4.71 MSPGRR862 pKa = 11.84 TLARR866 pKa = 11.84 HH867 pKa = 4.55 ATFYY871 pKa = 10.61 LTDD874 pKa = 3.15 SWRR877 pKa = 11.84 VNPTVGQAWGIPVLHH892 pKa = 5.88 STRR895 pKa = 11.84 SNVYY899 pKa = 8.67 WIRR902 pKa = 11.84 GINPDD907 pKa = 3.28 MPVIVSTVTAQNARR921 pKa = 11.84 RR922 pKa = 11.84 QYY924 pKa = 11.02 GEE926 pKa = 3.58 QAFTFSSCGGLDD938 pKa = 3.3 FPGYY942 pKa = 8.38 YY943 pKa = 9.91 QIVLTRR949 pKa = 11.84 EE950 pKa = 4.09 LLSAVPDD957 pKa = 4.02 DD958 pKa = 5.22 AIYY961 pKa = 8.98 TAFTRR966 pKa = 11.84 SRR968 pKa = 11.84 HH969 pKa = 6.24 DD970 pKa = 3.18 IAVLNALSPQEE981 pKa = 5.1 FRR983 pKa = 11.84 TACNNSRR990 pKa = 11.84 LLNALFNGVPLNGTYY1005 pKa = 10.14 RR1006 pKa = 11.84 DD1007 pKa = 4.0 VIGDD1011 pKa = 4.74 RR1012 pKa = 11.84 IPGVPLLGGFSLVHH1026 pKa = 5.55 GTRR1029 pKa = 11.84 LTTVADD1035 pKa = 3.51 VLGNDD1040 pKa = 3.38 GSKK1043 pKa = 10.84 YY1044 pKa = 10.97 DD1045 pKa = 3.79 NLEE1048 pKa = 4.68 PIMKK1052 pKa = 9.95 ASMLAPPPEE1061 pKa = 4.17 RR1062 pKa = 11.84 VEE1064 pKa = 4.62 SDD1066 pKa = 3.57 VSPVPLKK1073 pKa = 10.44 HH1074 pKa = 6.79 APPNDD1079 pKa = 3.23 EE1080 pKa = 3.8 WVPTYY1085 pKa = 10.5 FGRR1088 pKa = 11.84 APHH1091 pKa = 6.87 LKK1093 pKa = 10.11 NDD1095 pKa = 3.72 LNNTGFVRR1103 pKa = 11.84 EE1104 pKa = 4.06 NVEE1107 pKa = 3.95 FLSPDD1112 pKa = 3.13 GQMGRR1117 pKa = 11.84 MFGSDD1122 pKa = 4.01 RR1123 pKa = 11.84 KK1124 pKa = 10.34 PEE1126 pKa = 4.19 DD1127 pKa = 3.23 ASRR1130 pKa = 11.84 VDD1132 pKa = 3.32 GLNDD1136 pKa = 4.0 FFPLHH1141 pKa = 6.06 QSSDD1145 pKa = 3.49 PALWGPTVPKK1155 pKa = 10.17 RR1156 pKa = 11.84 LRR1158 pKa = 11.84 FGTEE1162 pKa = 3.09 QGNRR1166 pKa = 11.84 KK1167 pKa = 8.64 EE1168 pKa = 4.22 LADD1171 pKa = 3.98 SSFLGPMLCDD1181 pKa = 3.91 AFRR1184 pKa = 11.84 KK1185 pKa = 9.2 SHH1187 pKa = 6.36 NLPFDD1192 pKa = 3.84 IPWDD1196 pKa = 3.42 QEE1198 pKa = 4.0 LFDD1201 pKa = 5.09 RR1202 pKa = 11.84 CVEE1205 pKa = 4.06 DD1206 pKa = 3.95 CAQKK1210 pKa = 10.68 RR1211 pKa = 11.84 LSKK1214 pKa = 10.67 SAEE1217 pKa = 3.73 ALNNLTRR1224 pKa = 11.84 DD1225 pKa = 3.13 QDD1227 pKa = 4.32 PILRR1231 pKa = 11.84 DD1232 pKa = 3.38 NKK1234 pKa = 10.57 KK1235 pKa = 10.14 VLNFLKK1241 pKa = 10.72 GQLINKK1247 pKa = 9.24 LDD1249 pKa = 5.0 AITKK1253 pKa = 9.82 GSGAEE1258 pKa = 3.95 SFPTIKK1264 pKa = 9.47 PAQMITTYY1272 pKa = 9.91 TEE1274 pKa = 3.72 EE1275 pKa = 3.91 VNAAFGPYY1283 pKa = 9.02 IRR1285 pKa = 11.84 YY1286 pKa = 8.28 LACKK1290 pKa = 9.76 LRR1292 pKa = 11.84 EE1293 pKa = 4.05 HH1294 pKa = 6.83 SPDD1297 pKa = 3.08 HH1298 pKa = 5.96 VMHH1301 pKa = 6.43 YY1302 pKa = 11.16 GGMDD1306 pKa = 4.04 LNDD1309 pKa = 3.72 LGQWARR1315 pKa = 11.84 EE1316 pKa = 3.98 HH1317 pKa = 6.36 VPSKK1321 pKa = 9.0 RR1322 pKa = 11.84 TKK1324 pKa = 10.78 SFTNDD1329 pKa = 2.63 YY1330 pKa = 8.78 TAYY1333 pKa = 10.29 DD1334 pKa = 3.68 KK1335 pKa = 11.18 SCRR1338 pKa = 11.84 GEE1340 pKa = 3.87 TLAFEE1345 pKa = 4.26 ICIMRR1350 pKa = 11.84 LFNIPEE1356 pKa = 4.36 WIIEE1360 pKa = 4.0 LHH1362 pKa = 6.52 VEE1364 pKa = 4.04 LTLCVTSALGKK1375 pKa = 9.92 LGIMRR1380 pKa = 11.84 TSGQWCTYY1388 pKa = 10.52 LFNTWYY1394 pKa = 10.47 NDD1396 pKa = 2.97 AYY1398 pKa = 9.97 FTLKK1402 pKa = 10.7 YY1403 pKa = 10.25 VFPLNTPRR1411 pKa = 11.84 GFSGDD1416 pKa = 3.94 DD1417 pKa = 3.33 MFILCVPTISPGWLSISRR1435 pKa = 11.84 YY1436 pKa = 8.69 FSLVGKK1442 pKa = 8.68 PVITFLPEE1450 pKa = 3.51 FCGWLLTSHH1459 pKa = 7.07 GIIRR1463 pKa = 11.84 HH1464 pKa = 6.02 PYY1466 pKa = 8.91 LLYY1469 pKa = 10.87 AKK1471 pKa = 10.39 YY1472 pKa = 10.45 LFHH1475 pKa = 7.6 KK1476 pKa = 10.67 EE1477 pKa = 3.65 NGTLANVLISYY1488 pKa = 10.31 YY1489 pKa = 9.69 MEE1491 pKa = 4.38 FQHH1494 pKa = 7.19 SLTLGDD1500 pKa = 5.12 ALWEE1504 pKa = 4.1 SLPYY1508 pKa = 10.63 DD1509 pKa = 4.03 LLPYY1513 pKa = 10.12 QGQLQLLFNQARR1525 pKa = 11.84 SRR1527 pKa = 11.84 VPSFLIRR1534 pKa = 11.84 EE1535 pKa = 4.33 NKK1537 pKa = 10.52 LGDD1540 pKa = 3.49 APAAKK1545 pKa = 10.41 LSARR1549 pKa = 11.84 YY1550 pKa = 9.73 DD1551 pKa = 3.89 LFRR1554 pKa = 11.84 LPADD1558 pKa = 4.26 FMRR1561 pKa = 11.84 MDD1563 pKa = 3.32 WRR1565 pKa = 11.84 LLPSSIRR1572 pKa = 11.84 RR1573 pKa = 11.84 SLLLARR1579 pKa = 11.84 GLL1581 pKa = 3.77
Molecular weight: 176.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.387
IPC2_protein 7.775
IPC_protein 7.585
Toseland 7.366
ProMoST 8.17
Dawson 8.302
Bjellqvist 8.639
Wikipedia 8.244
Rodwell 8.331
Grimsley 7.483
Solomon 8.331
Lehninger 8.346
Nozaki 8.741
DTASelect 8.39
Thurlkill 8.434
EMBOSS 8.492
Sillero 8.726
Patrickios 4.406
IPC_peptide 8.331
IPC2_peptide 7.629
IPC2.peptide.svr19 7.72
Protein with the highest isoelectric point:
>tr|Q80QV2|Q80QV2_9VIRU RNA-dependent RNA polymerase OS=Oyster mushroom spherical virus OX=218667 PE=4 SV=1
MM1 pKa = 7.58 RR2 pKa = 11.84 FVLGTLHH9 pKa = 6.94 VIPISTPLSTFRR21 pKa = 11.84 TGAMVFCPTDD31 pKa = 3.55 TSPLPMKK38 pKa = 9.66 PLLMRR43 pKa = 11.84 RR44 pKa = 11.84 GCWTVAISPLMTVRR58 pKa = 11.84 TMLNFYY64 pKa = 10.22 CLTARR69 pKa = 11.84 TILSLLFQPMAMWFALTGFSMPLRR93 pKa = 11.84 WFSFQTSQVCPISPPPGSLLL113 pKa = 3.74
Molecular weight: 12.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.604
IPC_protein 10.789
Toseland 10.774
ProMoST 10.891
Dawson 10.862
Bjellqvist 10.745
Wikipedia 11.199
Rodwell 10.745
Grimsley 10.906
Solomon 11.155
Lehninger 11.082
Nozaki 10.862
DTASelect 10.716
Thurlkill 10.789
EMBOSS 11.228
Sillero 10.833
Patrickios 10.657
IPC_peptide 11.155
IPC2_peptide 10.379
IPC2.peptide.svr19 8.766
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2579
113
1581
368.4
40.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.755 ± 0.321
1.9 ± 0.634
4.071 ± 1.188
3.218 ± 0.891
5.118 ± 0.682
6.863 ± 1.212
2.21 ± 0.562
4.071 ± 0.276
2.675 ± 0.57
11.167 ± 1.331
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.21 ± 0.552
3.645 ± 0.649
8.802 ± 1.008
2.831 ± 0.387
7.367 ± 0.393
9.267 ± 1.641
6.475 ± 1.201
6.359 ± 0.734
1.59 ± 0.252
2.404 ± 0.718
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here