Streptococcus virus MS1
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9WR88|A0A1U9WR88_9CAUD Uncharacterized protein OS=Streptococcus virus MS1 OX=1962672 GN=MS1_33 PE=4 SV=1
MM1 pKa = 7.39 EE2 pKa = 5.86 RR3 pKa = 11.84 IKK5 pKa = 10.12 TLFHH9 pKa = 6.5 VIYY12 pKa = 11.06 NNGTHH17 pKa = 6.78 LEE19 pKa = 4.13 VAALFDD25 pKa = 4.58 TIDD28 pKa = 4.54 DD29 pKa = 3.67 YY30 pKa = 12.25 DD31 pKa = 3.82 EE32 pKa = 5.12 AVDD35 pKa = 5.27 KK36 pKa = 11.39 IEE38 pKa = 5.8 DD39 pKa = 4.22 YY40 pKa = 11.07 IDD42 pKa = 3.63 NPQSFNYY49 pKa = 9.94 KK50 pKa = 10.11 SIRR53 pKa = 11.84 LTPYY57 pKa = 10.4 NPDD60 pKa = 2.99 INGDD64 pKa = 3.87 VIATDD69 pKa = 3.0 ILLRR73 pKa = 11.84 LDD75 pKa = 4.43 DD76 pKa = 5.2 IIYY79 pKa = 10.64 VDD81 pKa = 4.85 ASCEE85 pKa = 4.09 TIKK88 pKa = 11.18 YY89 pKa = 8.87 EE90 pKa = 4.28 EE91 pKa = 4.41 PKK93 pKa = 10.91 AA94 pKa = 3.71
Molecular weight: 10.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.019
IPC2_protein 4.101
IPC_protein 4.062
Toseland 3.846
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.986
Rodwell 3.884
Grimsley 3.757
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.897
EMBOSS 3.999
Sillero 4.177
Patrickios 2.905
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.087
Protein with the highest isoelectric point:
>tr|A0A1U9WRA5|A0A1U9WRA5_9CAUD RecA OS=Streptococcus virus MS1 OX=1962672 GN=MS1_12 PE=3 SV=1
MM1 pKa = 7.35 AQRR4 pKa = 11.84 TLFSRR9 pKa = 11.84 PSGPKK14 pKa = 8.41 VSKK17 pKa = 9.88 PIKK20 pKa = 9.85 RR21 pKa = 11.84 RR22 pKa = 11.84 PKK24 pKa = 8.26 VQLDD28 pKa = 3.71 RR29 pKa = 11.84 EE30 pKa = 4.28 VLEE33 pKa = 5.1 LINRR37 pKa = 11.84 RR38 pKa = 11.84 QRR40 pKa = 11.84 QILVHH45 pKa = 6.19 SNLYY49 pKa = 9.46 YY50 pKa = 10.05 RR51 pKa = 11.84 QNVNLITDD59 pKa = 3.97 AQYY62 pKa = 11.59 DD63 pKa = 3.68 RR64 pKa = 11.84 WSHH67 pKa = 4.96 QLYY70 pKa = 10.85 DD71 pKa = 5.49 LIQDD75 pKa = 3.54 HH76 pKa = 6.81 PKK78 pKa = 10.56 EE79 pKa = 4.2 FRR81 pKa = 11.84 KK82 pKa = 10.14 SAWYY86 pKa = 9.22 EE87 pKa = 3.58 AFRR90 pKa = 11.84 TFDD93 pKa = 3.49 GNTGMGLPYY102 pKa = 9.72 TDD104 pKa = 3.57 PWVEE108 pKa = 4.11 GTATHH113 pKa = 7.15 LLKK116 pKa = 10.59 ISGGQPTT123 pKa = 3.85
Molecular weight: 14.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.443
IPC_protein 9.706
Toseland 9.97
ProMoST 9.75
Dawson 10.218
Bjellqvist 9.926
Wikipedia 10.423
Rodwell 10.482
Grimsley 10.306
Solomon 10.262
Lehninger 10.218
Nozaki 9.94
DTASelect 9.926
Thurlkill 10.058
EMBOSS 10.394
Sillero 10.131
Patrickios 9.926
IPC_peptide 10.262
IPC2_peptide 8.565
IPC2.peptide.svr19 8.521
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
17720
31
1226
230.1
26.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.896 ± 0.441
0.892 ± 0.124
6.23 ± 0.288
7.269 ± 0.426
3.984 ± 0.203
6.721 ± 0.355
1.524 ± 0.115
6.456 ± 0.274
7.156 ± 0.301
8.781 ± 0.329
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.619 ± 0.18
4.972 ± 0.256
3.476 ± 0.159
4.255 ± 0.192
4.644 ± 0.234
5.971 ± 0.247
6.078 ± 0.255
6.597 ± 0.185
1.287 ± 0.129
4.193 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here