Amino acid dipepetide frequency for Spirosoma sp. HMF3257

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.031AlaAla: 6.031 ± 0.078
0.699AlaCys: 0.699 ± 0.023
4.667AlaAsp: 4.667 ± 0.052
4.295AlaGlu: 4.295 ± 0.057
3.528AlaPhe: 3.528 ± 0.046
6.048AlaGly: 6.048 ± 0.08
1.384AlaHis: 1.384 ± 0.027
5.267AlaIle: 5.267 ± 0.064
4.081AlaLys: 4.081 ± 0.063
7.843AlaLeu: 7.843 ± 0.083
1.753AlaMet: 1.753 ± 0.033
3.851AlaAsn: 3.851 ± 0.048
2.9AlaPro: 2.9 ± 0.043
3.682AlaGln: 3.682 ± 0.047
3.494AlaArg: 3.494 ± 0.04
5.226AlaSer: 5.226 ± 0.076
5.48AlaThr: 5.48 ± 0.085
5.16AlaVal: 5.16 ± 0.065
0.912AlaTrp: 0.912 ± 0.025
3.131AlaTyr: 3.131 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.514CysAla: 0.514 ± 0.022
0.12CysCys: 0.12 ± 0.01
0.359CysAsp: 0.359 ± 0.015
0.372CysGlu: 0.372 ± 0.015
0.404CysPhe: 0.404 ± 0.016
0.586CysGly: 0.586 ± 0.023
0.197CysHis: 0.197 ± 0.01
0.494CysIle: 0.494 ± 0.018
0.319CysLys: 0.319 ± 0.013
0.94CysLeu: 0.94 ± 0.025
0.151CysMet: 0.151 ± 0.009
0.328CysAsn: 0.328 ± 0.014
0.399CysPro: 0.399 ± 0.017
0.421CysGln: 0.421 ± 0.017
0.431CysArg: 0.431 ± 0.017
0.678CysSer: 0.678 ± 0.031
0.525CysThr: 0.525 ± 0.027
0.513CysVal: 0.513 ± 0.021
0.131CysTrp: 0.131 ± 0.01
0.303CysTyr: 0.303 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
4.062AspAla: 4.062 ± 0.057
0.399AspCys: 0.399 ± 0.014
2.603AspAsp: 2.603 ± 0.047
3.248AspGlu: 3.248 ± 0.052
2.612AspPhe: 2.612 ± 0.04
3.851AspGly: 3.851 ± 0.059
0.885AspHis: 0.885 ± 0.024
3.047AspIle: 3.047 ± 0.046
2.981AspLys: 2.981 ± 0.042
5.199AspLeu: 5.199 ± 0.064
1.053AspMet: 1.053 ± 0.025
2.32AspAsn: 2.32 ± 0.042
2.328AspPro: 2.328 ± 0.044
2.31AspGln: 2.31 ± 0.04
2.647AspArg: 2.647 ± 0.04
3.037AspSer: 3.037 ± 0.045
2.724AspThr: 2.724 ± 0.037
3.439AspVal: 3.439 ± 0.049
0.875AspTrp: 0.875 ± 0.022
2.401AspTyr: 2.401 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
4.435GluAla: 4.435 ± 0.067
0.279GluCys: 0.279 ± 0.011
2.148GluAsp: 2.148 ± 0.039
2.873GluGlu: 2.873 ± 0.051
2.082GluPhe: 2.082 ± 0.037
3.092GluGly: 3.092 ± 0.046
1.011GluHis: 1.011 ± 0.022
3.19GluIle: 3.19 ± 0.046
3.527GluLys: 3.527 ± 0.053
5.651GluLeu: 5.651 ± 0.074
1.188GluMet: 1.188 ± 0.027
2.397GluAsn: 2.397 ± 0.037
1.999GluPro: 1.999 ± 0.039
2.715GluGln: 2.715 ± 0.049
3.058GluArg: 3.058 ± 0.051
2.909GluSer: 2.909 ± 0.048
3.193GluThr: 3.193 ± 0.042
3.49GluVal: 3.49 ± 0.051
0.682GluTrp: 0.682 ± 0.024
1.678GluTyr: 1.678 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
3.281PheAla: 3.281 ± 0.047
0.432PheCys: 0.432 ± 0.017
2.812PheAsp: 2.812 ± 0.041
2.209PheGlu: 2.209 ± 0.038
2.194PhePhe: 2.194 ± 0.038
3.292PheGly: 3.292 ± 0.048
0.796PheHis: 0.796 ± 0.019
2.804PheIle: 2.804 ± 0.041
1.977PheLys: 1.977 ± 0.04
4.39PheLeu: 4.39 ± 0.056
0.971PheMet: 0.971 ± 0.026
2.043PheAsn: 2.043 ± 0.037
1.775PhePro: 1.775 ± 0.034
1.677PheGln: 1.677 ± 0.031
2.397PheArg: 2.397 ± 0.037
3.393PheSer: 3.393 ± 0.048
3.205PheThr: 3.205 ± 0.051
2.958PheVal: 2.958 ± 0.036
0.663PheTrp: 0.663 ± 0.023
1.785PheTyr: 1.785 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
4.614GlyAla: 4.614 ± 0.058
0.781GlyCys: 0.781 ± 0.026
3.188GlyAsp: 3.188 ± 0.049
3.112GlyGlu: 3.112 ± 0.047
3.331GlyPhe: 3.331 ± 0.053
4.734GlyGly: 4.734 ± 0.069
1.341GlyHis: 1.341 ± 0.032
4.739GlyIle: 4.739 ± 0.057
4.295GlyLys: 4.295 ± 0.056
7.169GlyLeu: 7.169 ± 0.077
1.587GlyMet: 1.587 ± 0.031
3.301GlyAsn: 3.301 ± 0.056
2.086GlyPro: 2.086 ± 0.037
3.374GlyGln: 3.374 ± 0.047
3.227GlyArg: 3.227 ± 0.05
4.716GlySer: 4.716 ± 0.072
4.626GlyThr: 4.626 ± 0.071
4.601GlyVal: 4.601 ± 0.054
1.089GlyTrp: 1.089 ± 0.022
2.963GlyTyr: 2.963 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.165HisAla: 1.165 ± 0.028
0.214HisCys: 0.214 ± 0.01
0.912HisAsp: 0.912 ± 0.021
0.958HisGlu: 0.958 ± 0.026
1.031HisPhe: 1.031 ± 0.026
1.097HisGly: 1.097 ± 0.026
0.534HisHis: 0.534 ± 0.02
1.22HisIle: 1.22 ± 0.029
0.869HisLys: 0.869 ± 0.022
1.986HisLeu: 1.986 ± 0.033
0.369HisMet: 0.369 ± 0.015
0.749HisAsn: 0.749 ± 0.019
1.103HisPro: 1.103 ± 0.023
0.944HisGln: 0.944 ± 0.025
1.016HisArg: 1.016 ± 0.025
1.096HisSer: 1.096 ± 0.027
1.158HisThr: 1.158 ± 0.028
1.036HisVal: 1.036 ± 0.028
0.319HisTrp: 0.319 ± 0.013
0.879HisTyr: 0.879 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.269IleAla: 5.269 ± 0.058
0.603IleCys: 0.603 ± 0.018
3.738IleAsp: 3.738 ± 0.048
3.532IleGlu: 3.532 ± 0.052
2.248IlePhe: 2.248 ± 0.037
4.657IleGly: 4.657 ± 0.059
1.252IleHis: 1.252 ± 0.033
3.592IleIle: 3.592 ± 0.052
3.12IleLys: 3.12 ± 0.048
5.353IleLeu: 5.353 ± 0.066
1.092IleMet: 1.092 ± 0.025
3.202IleAsn: 3.202 ± 0.047
3.025IlePro: 3.025 ± 0.045
2.665IleGln: 2.665 ± 0.045
3.589IleArg: 3.589 ± 0.05
4.319IleSer: 4.319 ± 0.052
4.079IleThr: 4.079 ± 0.053
3.959IleVal: 3.959 ± 0.053
0.767IleTrp: 0.767 ± 0.024
2.114IleTyr: 2.114 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.712LysAla: 4.712 ± 0.066
0.236LysCys: 0.236 ± 0.012
2.668LysAsp: 2.668 ± 0.048
3.115LysGlu: 3.115 ± 0.061
1.742LysPhe: 1.742 ± 0.035
3.539LysGly: 3.539 ± 0.053
0.924LysHis: 0.924 ± 0.026
3.084LysIle: 3.084 ± 0.04
3.387LysLys: 3.387 ± 0.052
5.272LysLeu: 5.272 ± 0.059
1.301LysMet: 1.301 ± 0.031
2.571LysAsn: 2.571 ± 0.038
2.962LysPro: 2.962 ± 0.045
2.574LysGln: 2.574 ± 0.044
2.745LysArg: 2.745 ± 0.042
3.141LysSer: 3.141 ± 0.049
3.664LysThr: 3.664 ± 0.048
3.343LysVal: 3.343 ± 0.049
0.671LysTrp: 0.671 ± 0.019
1.814LysTyr: 1.814 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
8.655LeuAla: 8.655 ± 0.076
0.815LeuCys: 0.815 ± 0.021
4.832LeuAsp: 4.832 ± 0.067
4.641LeuGlu: 4.641 ± 0.06
4.709LeuPhe: 4.709 ± 0.067
6.081LeuGly: 6.081 ± 0.065
1.897LeuHis: 1.897 ± 0.039
6.456LeuIle: 6.456 ± 0.075
5.579LeuLys: 5.579 ± 0.067
11.015LeuLeu: 11.015 ± 0.129
2.236LeuMet: 2.236 ± 0.034
4.816LeuAsn: 4.816 ± 0.056
5.124LeuPro: 5.124 ± 0.063
3.904LeuGln: 3.904 ± 0.053
4.813LeuArg: 4.813 ± 0.059
7.27LeuSer: 7.27 ± 0.077
7.924LeuThr: 7.924 ± 0.091
6.623LeuVal: 6.623 ± 0.065
1.093LeuTrp: 1.093 ± 0.031
3.431LeuTyr: 3.431 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
2.042MetAla: 2.042 ± 0.034
0.134MetCys: 0.134 ± 0.009
1.005MetAsp: 1.005 ± 0.024
1.084MetGlu: 1.084 ± 0.025
0.606MetPhe: 0.606 ± 0.022
1.503MetGly: 1.503 ± 0.034
0.417MetHis: 0.417 ± 0.015
1.241MetIle: 1.241 ± 0.028
1.523MetLys: 1.523 ± 0.026
2.083MetLeu: 2.083 ± 0.041
0.502MetMet: 0.502 ± 0.019
1.212MetAsn: 1.212 ± 0.031
1.142MetPro: 1.142 ± 0.022
0.936MetGln: 0.936 ± 0.024
1.043MetArg: 1.043 ± 0.026
1.289MetSer: 1.289 ± 0.026
1.401MetThr: 1.401 ± 0.026
1.269MetVal: 1.269 ± 0.031
0.176MetTrp: 0.176 ± 0.01
0.565MetTyr: 0.565 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.499AsnAla: 3.499 ± 0.049
0.355AsnCys: 0.355 ± 0.016
2.433AsnAsp: 2.433 ± 0.038
2.479AsnGlu: 2.479 ± 0.036
1.995AsnPhe: 1.995 ± 0.029
3.817AsnGly: 3.817 ± 0.056
0.886AsnHis: 0.886 ± 0.023
2.399AsnIle: 2.399 ± 0.044
2.201AsnLys: 2.201 ± 0.034
4.651AsnLeu: 4.651 ± 0.063
0.878AsnMet: 0.878 ± 0.023
2.221AsnAsn: 2.221 ± 0.043
2.776AsnPro: 2.776 ± 0.043
2.518AsnGln: 2.518 ± 0.047
2.743AsnArg: 2.743 ± 0.047
2.852AsnSer: 2.852 ± 0.049
3.008AsnThr: 3.008 ± 0.066
2.923AsnVal: 2.923 ± 0.044
0.711AsnTrp: 0.711 ± 0.019
1.983AsnTyr: 1.983 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
4.063ProAla: 4.063 ± 0.052
0.251ProCys: 0.251 ± 0.013
3.16ProAsp: 3.16 ± 0.051
2.804ProGlu: 2.804 ± 0.041
2.087ProPhe: 2.087 ± 0.037
3.107ProGly: 3.107 ± 0.045
0.775ProHis: 0.775 ± 0.025
2.975ProIle: 2.975 ± 0.037
2.236ProLys: 2.236 ± 0.036
3.916ProLeu: 3.916 ± 0.051
0.915ProMet: 0.915 ± 0.023
2.315ProAsn: 2.315 ± 0.044
1.523ProPro: 1.523 ± 0.031
1.541ProGln: 1.541 ± 0.029
1.467ProArg: 1.467 ± 0.027
2.728ProSer: 2.728 ± 0.047
3.435ProThr: 3.435 ± 0.05
3.546ProVal: 3.546 ± 0.048
0.434ProTrp: 0.434 ± 0.015
1.621ProTyr: 1.621 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.847GlnAla: 3.847 ± 0.051
0.238GlnCys: 0.238 ± 0.012
1.688GlnAsp: 1.688 ± 0.031
2.172GlnGlu: 2.172 ± 0.04
2.071GlnPhe: 2.071 ± 0.035
2.429GlnGly: 2.429 ± 0.038
0.927GlnHis: 0.927 ± 0.024
2.688GlnIle: 2.688 ± 0.041
2.615GlnLys: 2.615 ± 0.041
5.143GlnLeu: 5.143 ± 0.069
0.952GlnMet: 0.952 ± 0.023
1.931GlnAsn: 1.931 ± 0.032
2.444GlnPro: 2.444 ± 0.037
2.704GlnGln: 2.704 ± 0.055
2.378GlnArg: 2.378 ± 0.041
2.753GlnSer: 2.753 ± 0.04
3.105GlnThr: 3.105 ± 0.05
2.889GlnVal: 2.889 ± 0.043
0.572GlnTrp: 0.572 ± 0.018
1.645GlnTyr: 1.645 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
3.367ArgAla: 3.367 ± 0.044
0.328ArgCys: 0.328 ± 0.016
2.336ArgAsp: 2.336 ± 0.04
2.677ArgGlu: 2.677 ± 0.044
2.511ArgPhe: 2.511 ± 0.042
2.417ArgGly: 2.417 ± 0.039
0.953ArgHis: 0.953 ± 0.023
3.458ArgIle: 3.458 ± 0.047
2.834ArgLys: 2.834 ± 0.043
5.527ArgLeu: 5.527 ± 0.062
1.285ArgMet: 1.285 ± 0.026
2.32ArgAsn: 2.32 ± 0.038
2.153ArgPro: 2.153 ± 0.041
2.755ArgGln: 2.755 ± 0.037
2.507ArgArg: 2.507 ± 0.043
2.921ArgSer: 2.921 ± 0.046
2.913ArgThr: 2.913 ± 0.049
3.25ArgVal: 3.25 ± 0.042
0.756ArgTrp: 0.756 ± 0.022
2.128ArgTyr: 2.128 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
4.973SerAla: 4.973 ± 0.071
0.668SerCys: 0.668 ± 0.025
3.304SerAsp: 3.304 ± 0.046
2.933SerGlu: 2.933 ± 0.039
3.293SerPhe: 3.293 ± 0.053
5.141SerGly: 5.141 ± 0.081
1.138SerHis: 1.138 ± 0.028
4.232SerIle: 4.232 ± 0.053
2.88SerLys: 2.88 ± 0.042
7.143SerLeu: 7.143 ± 0.082
1.298SerMet: 1.298 ± 0.03
2.709SerAsn: 2.709 ± 0.05
2.953SerPro: 2.953 ± 0.042
2.747SerGln: 2.747 ± 0.04
2.949SerArg: 2.949 ± 0.04
4.415SerSer: 4.415 ± 0.063
4.359SerThr: 4.359 ± 0.058
4.659SerVal: 4.659 ± 0.069
0.876SerTrp: 0.876 ± 0.025
2.591SerTyr: 2.591 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
5.546ThrAla: 5.546 ± 0.079
0.482ThrCys: 0.482 ± 0.018
3.857ThrAsp: 3.857 ± 0.049
3.131ThrGlu: 3.131 ± 0.044
3.089ThrPhe: 3.089 ± 0.041
5.382ThrGly: 5.382 ± 0.069
1.161ThrHis: 1.161 ± 0.026
4.5ThrIle: 4.5 ± 0.065
3.044ThrLys: 3.044 ± 0.044
6.762ThrLeu: 6.762 ± 0.068
1.154ThrMet: 1.154 ± 0.023
3.397ThrAsn: 3.397 ± 0.063
3.291ThrPro: 3.291 ± 0.046
2.41ThrGln: 2.41 ± 0.037
2.493ThrArg: 2.493 ± 0.039
4.249ThrSer: 4.249 ± 0.075
4.822ThrThr: 4.822 ± 0.099
4.79ThrVal: 4.79 ± 0.069
0.739ThrTrp: 0.739 ± 0.021
2.852ThrTyr: 2.852 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
5.617ValAla: 5.617 ± 0.069
0.679ValCys: 0.679 ± 0.021
3.601ValAsp: 3.601 ± 0.045
3.393ValGlu: 3.393 ± 0.049
2.912ValPhe: 2.912 ± 0.046
4.719ValGly: 4.719 ± 0.059
1.141ValHis: 1.141 ± 0.023
4.011ValIle: 4.011 ± 0.055
3.372ValLys: 3.372 ± 0.05
6.532ValLeu: 6.532 ± 0.063
1.451ValMet: 1.451 ± 0.029
3.163ValAsn: 3.163 ± 0.04
2.844ValPro: 2.844 ± 0.045
2.462ValGln: 2.462 ± 0.04
3.382ValArg: 3.382 ± 0.044
4.841ValSer: 4.841 ± 0.069
4.256ValThr: 4.256 ± 0.071
4.993ValVal: 4.993 ± 0.056
0.866ValTrp: 0.866 ± 0.023
2.42ValTyr: 2.42 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
0.954TrpAla: 0.954 ± 0.025
0.103TrpCys: 0.103 ± 0.007
0.613TrpAsp: 0.613 ± 0.019
0.623TrpGlu: 0.623 ± 0.02
0.63TrpPhe: 0.63 ± 0.019
0.797TrpGly: 0.797 ± 0.023
0.315TrpHis: 0.315 ± 0.013
0.745TrpIle: 0.745 ± 0.023
0.699TrpLys: 0.699 ± 0.02
1.605TrpLeu: 1.605 ± 0.036
0.342TrpMet: 0.342 ± 0.012
0.611TrpAsn: 0.611 ± 0.021
0.44TrpPro: 0.44 ± 0.015
0.772TrpGln: 0.772 ± 0.025
0.699TrpArg: 0.699 ± 0.027
0.8TrpSer: 0.8 ± 0.023
0.739TrpThr: 0.739 ± 0.019
0.891TrpVal: 0.891 ± 0.025
0.236TrpTrp: 0.236 ± 0.011
0.501TrpTyr: 0.501 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.785TyrAla: 2.785 ± 0.044
0.369TyrCys: 0.369 ± 0.016
2.095TyrAsp: 2.095 ± 0.035
1.947TyrGlu: 1.947 ± 0.035
1.977TyrPhe: 1.977 ± 0.041
2.585TyrGly: 2.585 ± 0.047
0.723TyrHis: 0.723 ± 0.021
2.034TyrIle: 2.034 ± 0.038
1.924TyrLys: 1.924 ± 0.038
3.737TyrLeu: 3.737 ± 0.054
0.694TyrMet: 0.694 ± 0.019
1.872TyrAsn: 1.872 ± 0.037
1.634TyrPro: 1.634 ± 0.034
2.08TyrGln: 2.08 ± 0.036
2.32TyrArg: 2.32 ± 0.04
2.629TyrSer: 2.629 ± 0.048
2.533TyrThr: 2.533 ± 0.051
2.322TyrVal: 2.322 ± 0.037
0.547TyrTrp: 0.547 ± 0.018
1.648TyrTyr: 1.648 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5857 proteins (1723158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski