Amino acid dipepetide frequency for Peribacillus asahii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.136AlaAla: 6.136 ± 0.093
0.585AlaCys: 0.585 ± 0.025
3.396AlaAsp: 3.396 ± 0.056
4.93AlaGlu: 4.93 ± 0.07
3.366AlaPhe: 3.366 ± 0.056
5.298AlaGly: 5.298 ± 0.079
1.332AlaHis: 1.332 ± 0.033
6.155AlaIle: 6.155 ± 0.091
5.024AlaLys: 5.024 ± 0.07
7.536AlaLeu: 7.536 ± 0.085
2.019AlaMet: 2.019 ± 0.039
2.885AlaAsn: 2.885 ± 0.046
2.113AlaPro: 2.113 ± 0.053
2.416AlaGln: 2.416 ± 0.051
2.725AlaArg: 2.725 ± 0.057
4.232AlaSer: 4.232 ± 0.068
3.653AlaThr: 3.653 ± 0.065
5.621AlaVal: 5.621 ± 0.085
0.617AlaTrp: 0.617 ± 0.024
2.408AlaTyr: 2.408 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.443CysAla: 0.443 ± 0.022
0.123CysCys: 0.123 ± 0.012
0.41CysAsp: 0.41 ± 0.018
0.474CysGlu: 0.474 ± 0.024
0.349CysPhe: 0.349 ± 0.016
0.692CysGly: 0.692 ± 0.027
0.201CysHis: 0.201 ± 0.013
0.571CysIle: 0.571 ± 0.024
0.369CysLys: 0.369 ± 0.018
0.695CysLeu: 0.695 ± 0.026
0.205CysMet: 0.205 ± 0.013
0.311CysAsn: 0.311 ± 0.019
0.336CysPro: 0.336 ± 0.018
0.26CysGln: 0.26 ± 0.014
0.273CysArg: 0.273 ± 0.016
0.522CysSer: 0.522 ± 0.022
0.428CysThr: 0.428 ± 0.018
0.446CysVal: 0.446 ± 0.022
0.071CysTrp: 0.071 ± 0.008
0.312CysTyr: 0.312 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.131AspAla: 3.131 ± 0.053
0.417AspCys: 0.417 ± 0.018
2.166AspAsp: 2.166 ± 0.046
4.094AspGlu: 4.094 ± 0.066
2.256AspPhe: 2.256 ± 0.043
3.053AspGly: 3.053 ± 0.061
0.979AspHis: 0.979 ± 0.028
4.12AspIle: 4.12 ± 0.067
2.868AspLys: 2.868 ± 0.052
4.744AspLeu: 4.744 ± 0.067
1.26AspMet: 1.26 ± 0.035
1.645AspAsn: 1.645 ± 0.039
1.715AspPro: 1.715 ± 0.038
1.779AspGln: 1.779 ± 0.044
2.014AspArg: 2.014 ± 0.04
2.61AspSer: 2.61 ± 0.05
2.413AspThr: 2.413 ± 0.046
3.695AspVal: 3.695 ± 0.048
0.597AspTrp: 0.597 ± 0.024
2.119AspTyr: 2.119 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.861GluAla: 5.861 ± 0.094
0.411GluCys: 0.411 ± 0.024
3.524GluAsp: 3.524 ± 0.059
7.324GluGlu: 7.324 ± 0.12
2.579GluPhe: 2.579 ± 0.043
4.376GluGly: 4.376 ± 0.066
1.55GluHis: 1.55 ± 0.034
5.524GluIle: 5.524 ± 0.073
6.4GluLys: 6.4 ± 0.096
7.235GluLeu: 7.235 ± 0.09
2.227GluMet: 2.227 ± 0.042
3.327GluAsn: 3.327 ± 0.054
1.933GluPro: 1.933 ± 0.041
3.875GluGln: 3.875 ± 0.065
3.615GluArg: 3.615 ± 0.058
3.47GluSer: 3.47 ± 0.062
3.863GluThr: 3.863 ± 0.06
5.211GluVal: 5.211 ± 0.073
0.828GluTrp: 0.828 ± 0.028
2.295GluTyr: 2.295 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.05PheAla: 3.05 ± 0.059
0.381PheCys: 0.381 ± 0.018
2.174PheAsp: 2.174 ± 0.047
2.858PheGlu: 2.858 ± 0.051
2.331PhePhe: 2.331 ± 0.052
3.224PheGly: 3.224 ± 0.061
0.971PheHis: 0.971 ± 0.03
3.983PheIle: 3.983 ± 0.068
2.307PheLys: 2.307 ± 0.043
4.637PheLeu: 4.637 ± 0.088
1.157PheMet: 1.157 ± 0.037
1.878PheAsn: 1.878 ± 0.039
1.687PhePro: 1.687 ± 0.039
1.638PheGln: 1.638 ± 0.037
1.51PheArg: 1.51 ± 0.038
3.142PheSer: 3.142 ± 0.052
2.596PheThr: 2.596 ± 0.047
3.176PheVal: 3.176 ± 0.045
0.469PheTrp: 0.469 ± 0.021
1.597PheTyr: 1.597 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
5.04GlyAla: 5.04 ± 0.077
0.647GlyCys: 0.647 ± 0.024
2.977GlyAsp: 2.977 ± 0.048
4.488GlyGlu: 4.488 ± 0.068
3.297GlyPhe: 3.297 ± 0.053
4.794GlyGly: 4.794 ± 0.085
1.397GlyHis: 1.397 ± 0.036
5.915GlyIle: 5.915 ± 0.084
5.019GlyLys: 5.019 ± 0.073
6.482GlyLeu: 6.482 ± 0.081
2.045GlyMet: 2.045 ± 0.046
2.562GlyAsn: 2.562 ± 0.045
1.724GlyPro: 1.724 ± 0.038
2.33GlyGln: 2.33 ± 0.045
2.685GlyArg: 2.685 ± 0.051
3.863GlySer: 3.863 ± 0.055
4.004GlyThr: 4.004 ± 0.067
5.138GlyVal: 5.138 ± 0.082
0.743GlyTrp: 0.743 ± 0.026
2.722GlyTyr: 2.722 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.3HisAla: 1.3 ± 0.038
0.182HisCys: 0.182 ± 0.014
0.968HisAsp: 0.968 ± 0.029
1.367HisGlu: 1.367 ± 0.033
1.025HisPhe: 1.025 ± 0.03
1.327HisGly: 1.327 ± 0.039
0.697HisHis: 0.697 ± 0.032
1.619HisIle: 1.619 ± 0.039
1.086HisLys: 1.086 ± 0.028
2.052HisLeu: 2.052 ± 0.05
0.511HisMet: 0.511 ± 0.02
0.824HisAsn: 0.824 ± 0.027
1.165HisPro: 1.165 ± 0.03
0.849HisGln: 0.849 ± 0.029
0.92HisArg: 0.92 ± 0.028
1.296HisSer: 1.296 ± 0.035
1.103HisThr: 1.103 ± 0.03
1.439HisVal: 1.439 ± 0.036
0.21HisTrp: 0.21 ± 0.014
0.887HisTyr: 0.887 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.262IleAla: 6.262 ± 0.082
0.69IleCys: 0.69 ± 0.023
4.09IleAsp: 4.09 ± 0.064
6.008IleGlu: 6.008 ± 0.069
3.206IlePhe: 3.206 ± 0.068
6.162IleGly: 6.162 ± 0.085
1.69IleHis: 1.69 ± 0.036
6.152IleIle: 6.152 ± 0.083
4.553IleLys: 4.553 ± 0.064
7.101IleLeu: 7.101 ± 0.091
1.867IleMet: 1.867 ± 0.037
3.213IleAsn: 3.213 ± 0.054
3.47IlePro: 3.47 ± 0.057
3.252IleGln: 3.252 ± 0.055
3.085IleArg: 3.085 ± 0.062
5.016IleSer: 5.016 ± 0.065
4.425IleThr: 4.425 ± 0.065
5.923IleVal: 5.923 ± 0.076
0.676IleTrp: 0.676 ± 0.023
2.401IleTyr: 2.401 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
4.863LysAla: 4.863 ± 0.071
0.312LysCys: 0.312 ± 0.018
3.417LysAsp: 3.417 ± 0.059
6.871LysGlu: 6.871 ± 0.095
1.894LysPhe: 1.894 ± 0.041
4.426LysGly: 4.426 ± 0.066
1.31LysHis: 1.31 ± 0.032
4.636LysIle: 4.636 ± 0.065
5.946LysLys: 5.946 ± 0.087
5.832LysLeu: 5.832 ± 0.083
2.176LysMet: 2.176 ± 0.044
3.105LysAsn: 3.105 ± 0.055
2.193LysPro: 2.193 ± 0.042
3.504LysGln: 3.504 ± 0.059
3.381LysArg: 3.381 ± 0.059
3.447LysSer: 3.447 ± 0.06
3.688LysThr: 3.688 ± 0.058
5.013LysVal: 5.013 ± 0.065
0.879LysTrp: 0.879 ± 0.03
2.203LysTyr: 2.203 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
7.642LeuAla: 7.642 ± 0.1
0.693LeuCys: 0.693 ± 0.026
4.658LeuAsp: 4.658 ± 0.051
6.688LeuGlu: 6.688 ± 0.09
4.937LeuPhe: 4.937 ± 0.096
6.464LeuGly: 6.464 ± 0.083
2.122LeuHis: 2.122 ± 0.041
7.189LeuIle: 7.189 ± 0.098
6.468LeuLys: 6.468 ± 0.081
10.21LeuLeu: 10.21 ± 0.13
2.425LeuMet: 2.425 ± 0.042
4.209LeuAsn: 4.209 ± 0.07
3.886LeuPro: 3.886 ± 0.064
4.086LeuGln: 4.086 ± 0.074
3.602LeuArg: 3.602 ± 0.063
6.702LeuSer: 6.702 ± 0.084
5.897LeuThr: 5.897 ± 0.076
6.317LeuVal: 6.317 ± 0.08
0.817LeuTrp: 0.817 ± 0.031
3.111LeuTyr: 3.111 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.015MetAla: 2.015 ± 0.044
0.175MetCys: 0.175 ± 0.014
1.401MetAsp: 1.401 ± 0.036
1.884MetGlu: 1.884 ± 0.045
1.058MetPhe: 1.058 ± 0.032
1.731MetGly: 1.731 ± 0.044
0.422MetHis: 0.422 ± 0.018
2.171MetIle: 2.171 ± 0.046
2.53MetLys: 2.53 ± 0.043
2.534MetLeu: 2.534 ± 0.051
0.88MetMet: 0.88 ± 0.03
1.51MetAsn: 1.51 ± 0.038
0.99MetPro: 0.99 ± 0.027
0.868MetGln: 0.868 ± 0.029
1.034MetArg: 1.034 ± 0.03
1.711MetSer: 1.711 ± 0.036
1.603MetThr: 1.603 ± 0.039
1.671MetVal: 1.671 ± 0.034
0.17MetTrp: 0.17 ± 0.012
0.768MetTyr: 0.768 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
2.67AsnAla: 2.67 ± 0.059
0.307AsnCys: 0.307 ± 0.017
2.01AsnAsp: 2.01 ± 0.043
3.273AsnGlu: 3.273 ± 0.054
1.576AsnPhe: 1.576 ± 0.038
3.189AsnGly: 3.189 ± 0.056
1.025AsnHis: 1.025 ± 0.03
3.43AsnIle: 3.43 ± 0.057
2.897AsnLys: 2.897 ± 0.05
3.745AsnLeu: 3.745 ± 0.06
1.171AsnMet: 1.171 ± 0.033
1.808AsnAsn: 1.808 ± 0.042
2.094AsnPro: 2.094 ± 0.051
2.079AsnGln: 2.079 ± 0.05
2.107AsnArg: 2.107 ± 0.049
2.263AsnSer: 2.263 ± 0.053
2.099AsnThr: 2.099 ± 0.044
2.926AsnVal: 2.926 ± 0.049
0.487AsnTrp: 0.487 ± 0.022
1.516AsnTyr: 1.516 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
2.399ProAla: 2.399 ± 0.045
0.204ProCys: 0.204 ± 0.013
1.875ProAsp: 1.875 ± 0.046
2.847ProGlu: 2.847 ± 0.049
1.962ProPhe: 1.962 ± 0.049
2.255ProGly: 2.255 ± 0.048
0.799ProHis: 0.799 ± 0.029
2.971ProIle: 2.971 ± 0.045
2.204ProLys: 2.204 ± 0.049
3.544ProLeu: 3.544 ± 0.062
0.821ProMet: 0.821 ± 0.028
1.682ProAsn: 1.682 ± 0.036
0.981ProPro: 0.981 ± 0.037
1.146ProGln: 1.146 ± 0.031
1.129ProArg: 1.129 ± 0.03
2.296ProSer: 2.296 ± 0.045
2.008ProThr: 2.008 ± 0.041
2.729ProVal: 2.729 ± 0.055
0.394ProTrp: 0.394 ± 0.018
1.407ProTyr: 1.407 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.111GlnAla: 3.111 ± 0.051
0.261GlnCys: 0.261 ± 0.014
1.663GlnAsp: 1.663 ± 0.037
3.09GlnGlu: 3.09 ± 0.052
1.667GlnPhe: 1.667 ± 0.035
2.266GlnGly: 2.266 ± 0.046
0.898GlnHis: 0.898 ± 0.031
2.746GlnIle: 2.746 ± 0.05
2.88GlnLys: 2.88 ± 0.049
4.379GlnLeu: 4.379 ± 0.069
1.164GlnMet: 1.164 ± 0.034
1.703GlnAsn: 1.703 ± 0.041
1.305GlnPro: 1.305 ± 0.036
2.181GlnGln: 2.181 ± 0.053
1.598GlnArg: 1.598 ± 0.041
2.378GlnSer: 2.378 ± 0.048
2.251GlnThr: 2.251 ± 0.05
2.648GlnVal: 2.648 ± 0.057
0.422GlnTrp: 0.422 ± 0.022
1.449GlnTyr: 1.449 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.545ArgAla: 2.545 ± 0.049
0.26ArgCys: 0.26 ± 0.014
2.045ArgAsp: 2.045 ± 0.037
3.288ArgGlu: 3.288 ± 0.057
1.916ArgPhe: 1.916 ± 0.046
2.364ArgGly: 2.364 ± 0.046
0.785ArgHis: 0.785 ± 0.026
3.115ArgIle: 3.115 ± 0.057
3.208ArgLys: 3.208 ± 0.05
3.937ArgLeu: 3.937 ± 0.064
1.258ArgMet: 1.258 ± 0.036
1.95ArgAsn: 1.95 ± 0.036
1.305ArgPro: 1.305 ± 0.035
1.537ArgGln: 1.537 ± 0.04
1.805ArgArg: 1.805 ± 0.039
2.171ArgSer: 2.171 ± 0.048
2.153ArgThr: 2.153 ± 0.038
2.61ArgVal: 2.61 ± 0.046
0.377ArgTrp: 0.377 ± 0.017
1.595ArgTyr: 1.595 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.803SerAla: 3.803 ± 0.059
0.444SerCys: 0.444 ± 0.022
2.561SerAsp: 2.561 ± 0.055
3.996SerGlu: 3.996 ± 0.062
3.319SerPhe: 3.319 ± 0.048
4.278SerGly: 4.278 ± 0.06
1.223SerHis: 1.223 ± 0.032
5.082SerIle: 5.082 ± 0.064
3.927SerLys: 3.927 ± 0.066
6.199SerLeu: 6.199 ± 0.077
1.695SerMet: 1.695 ± 0.037
2.489SerAsn: 2.489 ± 0.052
2.117SerPro: 2.117 ± 0.045
2.112SerGln: 2.112 ± 0.047
2.179SerArg: 2.179 ± 0.046
3.916SerSer: 3.916 ± 0.074
3.245SerThr: 3.245 ± 0.058
4.253SerVal: 4.253 ± 0.065
0.602SerTrp: 0.602 ± 0.023
2.28SerTyr: 2.28 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
4.225ThrAla: 4.225 ± 0.058
0.366ThrCys: 0.366 ± 0.02
2.589ThrAsp: 2.589 ± 0.047
3.679ThrGlu: 3.679 ± 0.06
2.629ThrPhe: 2.629 ± 0.047
4.251ThrGly: 4.251 ± 0.066
1.037ThrHis: 1.037 ± 0.028
4.756ThrIle: 4.756 ± 0.061
3.648ThrLys: 3.648 ± 0.061
5.49ThrLeu: 5.49 ± 0.072
1.298ThrMet: 1.298 ± 0.033
2.446ThrAsn: 2.446 ± 0.046
2.295ThrPro: 2.295 ± 0.044
1.499ThrGln: 1.499 ± 0.038
1.845ThrArg: 1.845 ± 0.037
3.389ThrSer: 3.389 ± 0.055
3.017ThrThr: 3.017 ± 0.06
4.283ThrVal: 4.283 ± 0.061
0.517ThrTrp: 0.517 ± 0.019
1.946ThrTyr: 1.946 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
5.251ValAla: 5.251 ± 0.076
0.63ValCys: 0.63 ± 0.022
3.502ValAsp: 3.502 ± 0.055
5.066ValGlu: 5.066 ± 0.072
3.155ValPhe: 3.155 ± 0.056
4.544ValGly: 4.544 ± 0.069
1.385ValHis: 1.385 ± 0.034
5.758ValIle: 5.758 ± 0.079
4.806ValLys: 4.806 ± 0.069
7.053ValLeu: 7.053 ± 0.077
1.791ValMet: 1.791 ± 0.038
3.12ValAsn: 3.12 ± 0.052
2.676ValPro: 2.676 ± 0.05
2.694ValGln: 2.694 ± 0.051
2.647ValArg: 2.647 ± 0.048
4.568ValSer: 4.568 ± 0.063
4.378ValThr: 4.378 ± 0.059
5.162ValVal: 5.162 ± 0.084
0.633ValTrp: 0.633 ± 0.024
2.382ValTyr: 2.382 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.599TrpAla: 0.599 ± 0.022
0.076TrpCys: 0.076 ± 0.008
0.465TrpAsp: 0.465 ± 0.02
0.629TrpGlu: 0.629 ± 0.024
0.519TrpPhe: 0.519 ± 0.023
0.696TrpGly: 0.696 ± 0.025
0.197TrpHis: 0.197 ± 0.015
0.808TrpIle: 0.808 ± 0.03
0.763TrpLys: 0.763 ± 0.024
1.189TrpLeu: 1.189 ± 0.037
0.316TrpMet: 0.316 ± 0.017
0.546TrpAsn: 0.546 ± 0.024
0.255TrpPro: 0.255 ± 0.017
0.359TrpGln: 0.359 ± 0.017
0.433TrpArg: 0.433 ± 0.02
0.582TrpSer: 0.582 ± 0.021
0.507TrpThr: 0.507 ± 0.023
0.658TrpVal: 0.658 ± 0.022
0.134TrpTrp: 0.134 ± 0.011
0.316TrpTyr: 0.316 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.101TyrAla: 2.101 ± 0.042
0.325TyrCys: 0.325 ± 0.017
1.785TyrAsp: 1.785 ± 0.039
2.576TyrGlu: 2.576 ± 0.051
1.724TyrPhe: 1.724 ± 0.046
2.483TyrGly: 2.483 ± 0.046
0.807TyrHis: 0.807 ± 0.021
2.542TyrIle: 2.542 ± 0.048
2.192TyrLys: 2.192 ± 0.046
3.528TyrLeu: 3.528 ± 0.057
0.839TyrMet: 0.839 ± 0.03
1.461TyrAsn: 1.461 ± 0.033
1.411TyrPro: 1.411 ± 0.04
1.596TyrGln: 1.596 ± 0.043
1.637TyrArg: 1.637 ± 0.041
2.146TyrSer: 2.146 ± 0.045
1.875TyrThr: 1.875 ± 0.044
2.283TyrVal: 2.283 ± 0.046
0.403TyrTrp: 0.403 ± 0.018
1.4TyrTyr: 1.4 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4015 proteins (1174495 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski