Histidinibacterium lentulum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Histidinibacterium

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4017 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2R8B2|A0A3N2R8B2_9RHOB ABC transporter permease OS=Histidinibacterium lentulum OX=2480588 GN=EAT49_05195 PE=4 SV=1
MM1 pKa = 6.72KK2 pKa = 9.93TSVSILAMAAATIGAPHH19 pKa = 6.78FAAAQDD25 pKa = 3.77APTIATVVKK34 pKa = 10.13IAGIQWFNRR43 pKa = 11.84MEE45 pKa = 4.29EE46 pKa = 4.29GVNQYY51 pKa = 11.1AADD54 pKa = 3.61TGANAFQVGPAQADD68 pKa = 3.76PQQQAALIEE77 pKa = 4.32DD78 pKa = 4.95MIAQGVDD85 pKa = 2.94ALAVVPMSPEE95 pKa = 3.51ALEE98 pKa = 4.31PVLGRR103 pKa = 11.84AMEE106 pKa = 3.92QGIVVITHH114 pKa = 6.45EE115 pKa = 4.85AASQQNTMYY124 pKa = 10.68DD125 pKa = 2.64IEE127 pKa = 4.88AFVNEE132 pKa = 4.48DD133 pKa = 3.2FGANLMEE140 pKa = 5.55QMAQCMGGEE149 pKa = 4.23GEE151 pKa = 4.26YY152 pKa = 10.91AVFVGSLTSQTHH164 pKa = 4.79NQWVDD169 pKa = 2.95GAIAHH174 pKa = 6.2QEE176 pKa = 3.63ANYY179 pKa = 10.48PNMTLVGDD187 pKa = 4.0KK188 pKa = 11.38NEE190 pKa = 4.42TFDD193 pKa = 5.46DD194 pKa = 4.03AQQAYY199 pKa = 8.66EE200 pKa = 3.91AAQEE204 pKa = 4.15VMRR207 pKa = 11.84AFPNVRR213 pKa = 11.84GMQGSASTDD222 pKa = 3.2VAGIGLAVEE231 pKa = 4.06EE232 pKa = 4.93RR233 pKa = 11.84GMEE236 pKa = 4.23DD237 pKa = 3.15ATCVFGTSLPSIAGQYY253 pKa = 10.54LEE255 pKa = 4.52TGAVDD260 pKa = 6.37GIGFWDD266 pKa = 4.36PSVAGYY272 pKa = 11.49AMNKK276 pKa = 7.23VAQMVMNGEE285 pKa = 4.26EE286 pKa = 4.33VTDD289 pKa = 4.41GMDD292 pKa = 3.86LGLEE296 pKa = 4.41GYY298 pKa = 10.7EE299 pKa = 4.53NVTLDD304 pKa = 3.28GKK306 pKa = 10.65VIYY309 pKa = 9.58GQAWVNVNADD319 pKa = 3.13NMADD323 pKa = 3.64YY324 pKa = 10.2PFF326 pKa = 5.35

Molecular weight: 34.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2R5D8|A0A3N2R5D8_9RHOB NAD-dependent epimerase/dehydratase family protein OS=Histidinibacterium lentulum OX=2480588 GN=EAT49_10345 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43GGRR28 pKa = 11.84KK29 pKa = 8.99VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.57SLCAA44 pKa = 3.82

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4017

0

4017

1290838

28

12941

321.3

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.439 ± 0.065

0.811 ± 0.016

5.99 ± 0.056

6.248 ± 0.04

3.486 ± 0.028

9.332 ± 0.067

1.956 ± 0.023

4.579 ± 0.031

2.095 ± 0.034

10.3 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.031

2.053 ± 0.028

5.558 ± 0.045

2.617 ± 0.02

7.814 ± 0.061

4.745 ± 0.042

5.481 ± 0.062

7.513 ± 0.043

1.47 ± 0.022

2.008 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski