Halomonas sp. YLB-10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N9U8K7|A0A3N9U8K7_9GAMM Sarcosine oxidase subunit alpha family protein OS=Halomonas sp. YLB-10 OX=2483111 GN=EBB56_02975 PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGAAAFTAAGAQAATVYY27 pKa = 10.61NQDD30 pKa = 3.01GTKK33 pKa = 10.39LDD35 pKa = 3.23IYY37 pKa = 11.24GNVQIVYY44 pKa = 10.4AGVKK48 pKa = 10.55DD49 pKa = 3.93EE50 pKa = 5.49DD51 pKa = 3.55GHH53 pKa = 7.56SRR55 pKa = 11.84DD56 pKa = 4.17EE57 pKa = 4.16IADD60 pKa = 3.65NGSTFGFAAEE70 pKa = 3.86HH71 pKa = 6.08VIYY74 pKa = 10.81NGLIGYY80 pKa = 9.5AKK82 pKa = 10.18IEE84 pKa = 4.05IDD86 pKa = 4.24DD87 pKa = 4.44FKK89 pKa = 11.48ADD91 pKa = 3.47EE92 pKa = 4.32MKK94 pKa = 10.94VAGRR98 pKa = 11.84DD99 pKa = 3.57AGDD102 pKa = 3.16TAYY105 pKa = 9.75IGLKK109 pKa = 10.68GNFGDD114 pKa = 5.02ARR116 pKa = 11.84IGSYY120 pKa = 11.06DD121 pKa = 3.67SLLDD125 pKa = 4.29DD126 pKa = 5.74WIQDD130 pKa = 3.78PISNNEE136 pKa = 3.8YY137 pKa = 10.85FDD139 pKa = 4.53VSDD142 pKa = 3.85SNTFKK147 pKa = 10.97GAPGEE152 pKa = 4.43DD153 pKa = 3.68ADD155 pKa = 4.68DD156 pKa = 4.53ASVVAGDD163 pKa = 4.12PEE165 pKa = 4.71GDD167 pKa = 3.53KK168 pKa = 9.7ITYY171 pKa = 7.65TSPVWGGFQFALGTQYY187 pKa = 11.23KK188 pKa = 10.58GDD190 pKa = 3.99AEE192 pKa = 4.29NEE194 pKa = 3.94NLTDD198 pKa = 3.75GGEE201 pKa = 3.81ASIFGGLKK209 pKa = 8.16YY210 pKa = 9.34TIGGFSVAGVYY221 pKa = 10.53DD222 pKa = 3.76DD223 pKa = 5.34LGIYY227 pKa = 9.99DD228 pKa = 5.0IEE230 pKa = 4.52DD231 pKa = 3.19QSVSYY236 pKa = 11.02LSTDD240 pKa = 3.04VAADD244 pKa = 3.6GTVTTTQNNVTIDD257 pKa = 4.04DD258 pKa = 4.3EE259 pKa = 5.09DD260 pKa = 5.97LGDD263 pKa = 3.65QYY265 pKa = 11.56GVTMQYY271 pKa = 10.08QWDD274 pKa = 4.0SLRR277 pKa = 11.84VAVKK281 pKa = 9.05YY282 pKa = 10.67EE283 pKa = 3.95RR284 pKa = 11.84FEE286 pKa = 4.35SDD288 pKa = 3.28NDD290 pKa = 3.54FLKK293 pKa = 11.15DD294 pKa = 3.08ADD296 pKa = 4.07YY297 pKa = 10.92YY298 pKa = 11.51AIGARR303 pKa = 11.84YY304 pKa = 9.66GYY306 pKa = 10.32LNGMGDD312 pKa = 3.04IYY314 pKa = 11.2GSYY317 pKa = 10.48QYY319 pKa = 10.71IDD321 pKa = 3.53LGGSGFSDD329 pKa = 3.75FDD331 pKa = 4.12SANDD335 pKa = 5.25DD336 pKa = 4.5EE337 pKa = 6.45NDD339 pKa = 3.41DD340 pKa = 4.83DD341 pKa = 4.66NFNEE345 pKa = 4.15VVAGVTYY352 pKa = 10.62NISDD356 pKa = 3.12AMYY359 pKa = 8.48TYY361 pKa = 11.28VEE363 pKa = 4.17GAVRR367 pKa = 11.84DD368 pKa = 3.88RR369 pKa = 11.84DD370 pKa = 3.44NDD372 pKa = 3.44QGDD375 pKa = 4.49GYY377 pKa = 11.52AVGLTYY383 pKa = 11.05LFF385 pKa = 4.66

Molecular weight: 41.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N9U1D3|A0A3N9U1D3_9GAMM Transcriptional regulator OS=Halomonas sp. YLB-10 OX=2483111 GN=EBB56_13285 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4189

0

4189

1430958

28

9628

341.6

37.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.248 ± 0.047

0.951 ± 0.014

6.239 ± 0.066

6.303 ± 0.042

3.366 ± 0.027

8.482 ± 0.049

2.354 ± 0.022

4.524 ± 0.03

2.517 ± 0.029

11.371 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.028

2.427 ± 0.025

4.893 ± 0.036

3.745 ± 0.025

7.128 ± 0.053

5.93 ± 0.043

5.037 ± 0.046

7.368 ± 0.033

1.44 ± 0.017

2.262 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski