Apis mellifera associated microvirus 39

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UU29|A0A3S8UU29_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 39 OX=2494768 PE=4 SV=1
MM1 pKa = 7.12ATKK4 pKa = 10.58DD5 pKa = 3.21SDD7 pKa = 3.93DD8 pKa = 3.58QVGPKK13 pKa = 9.94QPVYY17 pKa = 10.47PRR19 pKa = 11.84LDD21 pKa = 3.5EE22 pKa = 5.3HH23 pKa = 6.43GHH25 pKa = 5.35EE26 pKa = 4.65VPDD29 pKa = 4.42PRR31 pKa = 11.84PMAIPAGFRR40 pKa = 11.84RR41 pKa = 11.84PEE43 pKa = 3.77TLAEE47 pKa = 3.87QVQRR51 pKa = 11.84LVRR54 pKa = 11.84THH56 pKa = 7.6ISMQAQNEE64 pKa = 4.31GFEE67 pKa = 4.44SFEE70 pKa = 3.95EE71 pKa = 5.2AEE73 pKa = 4.73DD74 pKa = 4.06FDD76 pKa = 5.8IEE78 pKa = 4.59DD79 pKa = 3.77DD80 pKa = 3.76HH81 pKa = 8.74GEE83 pKa = 3.91PTTPYY88 pKa = 8.58EE89 pKa = 4.29TEE91 pKa = 3.81FDD93 pKa = 3.67PVLGRR98 pKa = 11.84EE99 pKa = 3.83ITPAEE104 pKa = 4.07FSLHH108 pKa = 4.06QEE110 pKa = 4.14RR111 pKa = 11.84YY112 pKa = 8.22RR113 pKa = 11.84RR114 pKa = 11.84EE115 pKa = 3.86MLDD118 pKa = 2.93RR119 pKa = 11.84QARR122 pKa = 11.84HH123 pKa = 5.2FARR126 pKa = 11.84RR127 pKa = 11.84DD128 pKa = 3.43LEE130 pKa = 4.59HH131 pKa = 7.12EE132 pKa = 4.39VAQPRR137 pKa = 11.84SRR139 pKa = 11.84KK140 pKa = 9.35QPAKK144 pKa = 10.64DD145 pKa = 3.74AGDD148 pKa = 4.1PPAKK152 pKa = 9.64PAAKK156 pKa = 9.7EE157 pKa = 4.08PPSPTGG163 pKa = 3.16

Molecular weight: 18.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU38|A0A3S8UU38_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 39 OX=2494768 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84NGGFTRR8 pKa = 11.84SFRR11 pKa = 11.84STAFAKK17 pKa = 10.35RR18 pKa = 11.84PARR21 pKa = 11.84MCPRR25 pKa = 11.84AGQPSQGHH33 pKa = 5.42HH34 pKa = 6.46RR35 pKa = 11.84RR36 pKa = 11.84TAMRR40 pKa = 11.84YY41 pKa = 7.21RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84SLRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84SRR53 pKa = 11.84VRR55 pKa = 11.84RR56 pKa = 11.84LRR58 pKa = 11.84RR59 pKa = 11.84GRR61 pKa = 11.84GRR63 pKa = 11.84PMRR66 pKa = 11.84IGFRR70 pKa = 11.84MM71 pKa = 3.48

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1456

71

506

242.7

27.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.478 ± 1.344

0.687 ± 0.39

4.602 ± 0.615

5.838 ± 0.944

3.64 ± 0.393

7.692 ± 0.622

2.473 ± 0.394

3.915 ± 0.558

3.709 ± 0.373

7.624 ± 1.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.923 ± 0.44

2.816 ± 0.608

7.486 ± 1.021

5.288 ± 0.784

10.234 ± 2.93

6.525 ± 0.801

5.907 ± 0.839

5.426 ± 1.012

1.717 ± 0.433

3.022 ± 0.972
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski