Streptococcus phage phi-SgaBSJ27_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MLW1|A0A7G3MLW1_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SgaBSJ27_rum OX=2664388 PE=4 SV=1
MM1 pKa = 7.18NLKK4 pKa = 10.5DD5 pKa = 3.46ILEE8 pKa = 4.74LGTCGFNPDD17 pKa = 3.53CKK19 pKa = 11.14VEE21 pKa = 4.07IFCMDD26 pKa = 3.25NFEE29 pKa = 5.62DD30 pKa = 3.93RR31 pKa = 11.84LEE33 pKa = 4.3NEE35 pKa = 4.49GFDD38 pKa = 5.31EE39 pKa = 5.2ILIPQNEE46 pKa = 4.18DD47 pKa = 2.73AKK49 pKa = 10.24IYY51 pKa = 9.99PYY53 pKa = 11.33AFLIEE58 pKa = 4.24DD59 pKa = 4.28SILIAMTEE67 pKa = 3.91EE68 pKa = 4.47DD69 pKa = 5.42DD70 pKa = 4.4NTNEE74 pKa = 4.2CC75 pKa = 4.51

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MQY8|A0A7G3MQY8_9CAUD Tail length tape-measure protein OS=Streptococcus phage phi-SgaBSJ27_rum OX=2664388 PE=4 SV=1
MM1 pKa = 7.73PRR3 pKa = 11.84RR4 pKa = 11.84PSIPCKK10 pKa = 10.5QNGCPNLVSYY20 pKa = 7.88GQKK23 pKa = 9.68YY24 pKa = 9.61CEE26 pKa = 3.77NHH28 pKa = 6.52KK29 pKa = 10.98ANHH32 pKa = 5.69QLDD35 pKa = 4.16SKK37 pKa = 8.65STKK40 pKa = 10.13AKK42 pKa = 10.34GYY44 pKa = 8.38NAQWNKK50 pKa = 10.15ARR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 9.99LKK57 pKa = 9.81VHH59 pKa = 6.46PLCVQCKK66 pKa = 9.75AKK68 pKa = 10.52GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.97RR84 pKa = 11.84GDD86 pKa = 5.06QEE88 pKa = 5.0LFWNQTNWQALCKK101 pKa = 9.93SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.13TTDD112 pKa = 2.64RR113 pKa = 11.84YY114 pKa = 10.3VEE116 pKa = 3.73YY117 pKa = 10.38SYY119 pKa = 11.5RR120 pKa = 11.84FF121 pKa = 3.35

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

32977

39

1820

297.1

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.701 ± 0.267

0.794 ± 0.092

6.232 ± 0.215

7.287 ± 0.225

4.282 ± 0.179

6.016 ± 0.209

1.819 ± 0.079

6.887 ± 0.29

7.508 ± 0.227

9.619 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.14

5.219 ± 0.158

2.884 ± 0.175

4.433 ± 0.155

4.418 ± 0.215

6.887 ± 0.214

6.198 ± 0.329

6.48 ± 0.23

1.04 ± 0.075

4.112 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski