Human polyomavirus 12

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4T5D3|M4T5D3_9POLY Capsid protein VP1 OS=Human polyomavirus 12 OX=1303334 GN=VP1 PE=1 SV=2
MM1 pKa = 7.37APKK4 pKa = 10.47RR5 pKa = 11.84KK6 pKa = 6.14TTCSSKK12 pKa = 9.45KK13 pKa = 8.44TCPQPSSVPKK23 pKa = 10.51LIIKK27 pKa = 10.25GGIEE31 pKa = 3.93VLDD34 pKa = 4.01VKK36 pKa = 10.35TGDD39 pKa = 4.0DD40 pKa = 4.31SITQIEE46 pKa = 4.54AFLNPRR52 pKa = 11.84MGVNDD57 pKa = 4.35EE58 pKa = 4.21TNTWYY63 pKa = 10.59GFSEE67 pKa = 4.38QVTVATARR75 pKa = 11.84EE76 pKa = 3.94TDD78 pKa = 3.64RR79 pKa = 11.84PPKK82 pKa = 10.11EE83 pKa = 3.54QMPYY87 pKa = 9.5YY88 pKa = 9.62SCARR92 pKa = 11.84IPLPLLNEE100 pKa = 4.49DD101 pKa = 3.74MTCNTLLMWEE111 pKa = 4.19AVSVKK116 pKa = 10.22TEE118 pKa = 4.18VIGSNTLMNVHH129 pKa = 7.52DD130 pKa = 4.39YY131 pKa = 7.47MTRR134 pKa = 11.84TDD136 pKa = 3.72NGVGHH141 pKa = 6.66PVVGSTYY148 pKa = 11.08HH149 pKa = 5.27MFAVGGEE156 pKa = 3.99PLDD159 pKa = 3.7LQGIQQSHH167 pKa = 6.0LVQYY171 pKa = 10.09PEE173 pKa = 3.97GLIVPKK179 pKa = 10.65SVTDD183 pKa = 3.39VTAKK187 pKa = 10.0IQCLDD192 pKa = 3.64PSAKK196 pKa = 10.4AKK198 pKa = 10.26LDD200 pKa = 3.43KK201 pKa = 10.76DD202 pKa = 3.46GKK204 pKa = 10.83YY205 pKa = 10.26PIEE208 pKa = 4.22TWSPDD213 pKa = 3.05PSRR216 pKa = 11.84NEE218 pKa = 3.42NTRR221 pKa = 11.84YY222 pKa = 9.46FGNYY226 pKa = 9.01YY227 pKa = 10.64GGLTTPPVLTFTNTVTTILLDD248 pKa = 3.88EE249 pKa = 5.0NGVGPLCKK257 pKa = 10.08GDD259 pKa = 4.6GLFLSCCDD267 pKa = 3.3VMGWFTAGSGTHH279 pKa = 4.67QRR281 pKa = 11.84FRR283 pKa = 11.84GLPRR287 pKa = 11.84YY288 pKa = 10.0FNVQLRR294 pKa = 11.84KK295 pKa = 9.21RR296 pKa = 11.84AVRR299 pKa = 11.84NPYY302 pKa = 9.14PVSALLTSLFTNMMPRR318 pKa = 11.84MSGQPMIGNKK328 pKa = 9.41SQVEE332 pKa = 4.1EE333 pKa = 4.0VRR335 pKa = 11.84VYY337 pKa = 10.85EE338 pKa = 4.78GLEE341 pKa = 3.9QLPGDD346 pKa = 4.14PDD348 pKa = 3.59MEE350 pKa = 4.08RR351 pKa = 11.84HH352 pKa = 5.77IDD354 pKa = 3.57EE355 pKa = 5.26FGQEE359 pKa = 4.0ITPVPP364 pKa = 3.72

Molecular weight: 40.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4STH5|M4STH5_9POLY Large T antigen OS=Human polyomavirus 12 OX=1303334 PE=4 SV=1
MM1 pKa = 8.27DD2 pKa = 6.0SILTFAEE9 pKa = 4.02RR10 pKa = 11.84QLLISLLKK18 pKa = 10.52ISGDD22 pKa = 3.44TFGNVPAMARR32 pKa = 11.84AYY34 pKa = 9.7KK35 pKa = 10.24LAAKK39 pKa = 10.03RR40 pKa = 11.84LHH42 pKa = 6.69PDD44 pKa = 2.56KK45 pKa = 11.14GGNEE49 pKa = 3.97AEE51 pKa = 4.11MKK53 pKa = 10.37KK54 pKa = 10.82LNEE57 pKa = 3.92LWNKK61 pKa = 9.79FKK63 pKa = 11.22DD64 pKa = 3.97GIYY67 pKa = 10.41NLRR70 pKa = 11.84EE71 pKa = 4.3VKK73 pKa = 10.27PSLHH77 pKa = 6.26PVVTCTVLGARR88 pKa = 11.84NIFNLITNSSQCMRR102 pKa = 11.84NLLRR106 pKa = 11.84YY107 pKa = 8.49CRR109 pKa = 11.84CFCCILFQQHH119 pKa = 5.59RR120 pKa = 11.84QLKK123 pKa = 6.75ITYY126 pKa = 9.65RR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84CNVWGQCYY137 pKa = 10.14CFLCYY142 pKa = 9.12YY143 pKa = 9.47TWFGVNCSIGAFTEE157 pKa = 3.93WLILLKK163 pKa = 10.81HH164 pKa = 6.36LDD166 pKa = 3.17WRR168 pKa = 11.84LLKK171 pKa = 10.39ISSAEE176 pKa = 3.79LDD178 pKa = 3.84VLGKK182 pKa = 10.4

Molecular weight: 21.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1764

182

708

352.8

39.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.519 ± 1.188

2.324 ± 0.74

5.045 ± 0.702

5.669 ± 0.358

4.025 ± 0.672

7.313 ± 0.743

2.494 ± 0.25

5.612 ± 0.401

6.066 ± 1.307

9.694 ± 1.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.685 ± 0.498

4.592 ± 0.754

5.612 ± 0.766

4.252 ± 0.588

5.215 ± 0.707

5.896 ± 0.545

5.839 ± 0.837

5.782 ± 0.665

1.587 ± 0.524

2.778 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski