Alteribacillus bidgolensis
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4578 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8LCK3|A0A1G8LCK3_9BACI Regulatory protein RecX OS=Alteribacillus bidgolensis OX=930129 GN=recX PE=3 SV=1
MM1 pKa = 7.58 EE2 pKa = 5.96 KK3 pKa = 10.48 YY4 pKa = 10.41 LKK6 pKa = 9.51 RR7 pKa = 11.84 TLLSAVSGAGILFAAACGGSDD28 pKa = 3.2 EE29 pKa = 4.99 SGAEE33 pKa = 4.17 GEE35 pKa = 4.71 ADD37 pKa = 3.21 GSADD41 pKa = 3.57 GEE43 pKa = 4.37 EE44 pKa = 4.25 QASEE48 pKa = 4.02 EE49 pKa = 4.52 GISYY53 pKa = 10.83 DD54 pKa = 5.03 DD55 pKa = 3.84 YY56 pKa = 11.44 PSTVKK61 pKa = 10.36 IGTASQGGTYY71 pKa = 9.84 YY72 pKa = 10.18 IYY74 pKa = 11.02 GGGIGSLLEE83 pKa = 4.18 SEE85 pKa = 5.23 LDD87 pKa = 2.87 ITANVEE93 pKa = 4.17 VTGGPVHH100 pKa = 6.91 NMQLTNGKK108 pKa = 8.95 DD109 pKa = 3.26 QDD111 pKa = 3.59 IGLVTLGPGYY121 pKa = 10.63 EE122 pKa = 4.26 GVTGTGEE129 pKa = 3.75 WTEE132 pKa = 4.17 GNKK135 pKa = 9.23 MEE137 pKa = 4.64 DD138 pKa = 3.4 VRR140 pKa = 11.84 VTFPMYY146 pKa = 7.61 TTPFHH151 pKa = 5.68 WWSLEE156 pKa = 3.65 GSGVEE161 pKa = 5.57 SIDD164 pKa = 5.06 DD165 pKa = 3.65 MDD167 pKa = 3.74 GMKK170 pKa = 10.55 VGVGPAGGTSGTYY183 pKa = 10.42 LPLIHH188 pKa = 7.64 DD189 pKa = 4.4 ALEE192 pKa = 4.18 LNIDD196 pKa = 3.89 PVQAGASDD204 pKa = 3.84 MVSQQMDD211 pKa = 3.47 GQLDD215 pKa = 4.2 VIGFAAGIPISAVSEE230 pKa = 4.26 VEE232 pKa = 3.77 AQRR235 pKa = 11.84 DD236 pKa = 3.62 INFFGIDD243 pKa = 3.25 GEE245 pKa = 4.21 QRR247 pKa = 11.84 EE248 pKa = 4.69 KK249 pKa = 10.49 IIEE252 pKa = 4.16 EE253 pKa = 3.85 YY254 pKa = 10.07 PYY256 pKa = 10.35 FFEE259 pKa = 4.23 YY260 pKa = 10.17 TIPADD265 pKa = 3.59 TYY267 pKa = 10.16 NQLDD271 pKa = 3.88 EE272 pKa = 4.81 DD273 pKa = 4.77 LEE275 pKa = 4.6 TIAMFNFGIVHH286 pKa = 6.39 KK287 pKa = 9.56 EE288 pKa = 3.65 ANEE291 pKa = 3.84 EE292 pKa = 3.85 FVYY295 pKa = 11.03 DD296 pKa = 3.71 LVKK299 pKa = 10.46 AYY301 pKa = 10.57 HH302 pKa = 6.35 EE303 pKa = 4.31 NQEE306 pKa = 4.26 KK307 pKa = 10.81 LMDD310 pKa = 3.5 THH312 pKa = 6.83 SAAEE316 pKa = 3.89 EE317 pKa = 4.19 AEE319 pKa = 4.1 IEE321 pKa = 4.91 AILQNDD327 pKa = 4.07 VMPLHH332 pKa = 6.91 PGAIKK337 pKa = 10.07 FYY339 pKa = 9.36 EE340 pKa = 4.26 EE341 pKa = 4.16 EE342 pKa = 5.65 GIDD345 pKa = 4.15 LPEE348 pKa = 4.7 DD349 pKa = 3.7 VYY351 pKa = 11.18 PPEE354 pKa = 4.04 WEE356 pKa = 4.01 EE357 pKa = 3.77
Molecular weight: 38.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.643
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.062
Thurlkill 3.668
EMBOSS 3.694
Sillero 3.948
Patrickios 1.049
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A1G8LQH5|A0A1G8LQH5_9BACI S4 domain protein YaaA OS=Alteribacillus bidgolensis OX=930129 GN=SAMN05216352_10930 PE=4 SV=1
MM1 pKa = 7.67 GKK3 pKa = 8.0 PTFKK7 pKa = 10.53 PNNRR11 pKa = 11.84 KK12 pKa = 9.27 RR13 pKa = 11.84 KK14 pKa = 8.26 KK15 pKa = 8.69 VHH17 pKa = 5.46 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 10.26 NGRR29 pKa = 11.84 HH30 pKa = 3.85 VLKK33 pKa = 10.52 NRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.66 GRR40 pKa = 11.84 KK41 pKa = 8.69 VLSAA45 pKa = 4.05
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4578
0
4578
1244654
27
1533
271.9
30.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.23 ± 0.041
0.738 ± 0.01
5.11 ± 0.031
8.062 ± 0.049
4.515 ± 0.031
7.006 ± 0.033
2.224 ± 0.017
7.422 ± 0.032
6.661 ± 0.038
9.413 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.861 ± 0.017
4.451 ± 0.026
3.783 ± 0.021
3.667 ± 0.027
4.019 ± 0.025
6.088 ± 0.027
5.403 ± 0.021
6.866 ± 0.029
1.087 ± 0.015
3.393 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here