Rhodoplanes piscinae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Rhodoplanes

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327JHY4|A0A327JHY4_9RHIZ LacI family transcriptional regulator (Fragment) OS=Rhodoplanes piscinae OX=444923 GN=CH340_25405 PE=3 SV=1
YYY2 pKa = 10.22DDD4 pKa = 3.5CAYYY8 pKa = 10.72EEE10 pKa = 3.87GEEE13 pKa = 4.12EEE15 pKa = 4.52EE16 pKa = 5.19EEE18 pKa = 4.39HH19 pKa = 5.26YY20 pKa = 9.83PVKKK24 pKa = 10.58GGPHHH29 pKa = 5.07LEEE32 pKa = 4.14VRR34 pKa = 11.84PSCSACNRR42 pKa = 11.84AKKK45 pKa = 10.67DDD47 pKa = 3.73DDD49 pKa = 3.51EEE51 pKa = 4.02EE52 pKa = 4.29LAEEE56 pKa = 4.0AEEE59 pKa = 4.37DDD61 pKa = 4.32LTEEE65 pKa = 4.28DD66 pKa = 4.03EE67 pKa = 4.7NALIDDD73 pKa = 4.05EEE75 pKa = 4.02AKKK78 pKa = 10.0YY79 pKa = 10.77NEEE82 pKa = 4.36DD83 pKa = 3.12

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327JW23|A0A327JW23_9RHIZ DNA helicase (Fragment) OS=Rhodoplanes piscinae OX=444923 GN=CH340_20705 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43GGRR28 pKa = 11.84QVLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5004

0

5004

1472701

25

2081

294.3

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.218 ± 0.054

0.839 ± 0.01

5.694 ± 0.028

5.225 ± 0.033

3.368 ± 0.023

8.825 ± 0.032

1.993 ± 0.018

4.529 ± 0.022

2.588 ± 0.031

10.141 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.016

1.951 ± 0.017

5.883 ± 0.034

2.621 ± 0.022

8.171 ± 0.036

4.55 ± 0.023

5.47 ± 0.026

8.432 ± 0.031

1.298 ± 0.016

1.976 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski