Anatilimnocola aggregata

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Anatilimnocola

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517YLX2|A0A517YLX2_9BACT Alpha-D-kanosaminyltransferase OS=Anatilimnocola aggregata OX=2528021 GN=kanE_2 PE=4 SV=1
MM1 pKa = 7.61SRR3 pKa = 11.84INNRR7 pKa = 11.84NRR9 pKa = 11.84NHH11 pKa = 6.62SKK13 pKa = 9.81PAPVPRR19 pKa = 11.84WRR21 pKa = 11.84RR22 pKa = 11.84GVIEE26 pKa = 4.12KK27 pKa = 10.96LEE29 pKa = 4.0ARR31 pKa = 11.84EE32 pKa = 4.15VFSGTPLPVLLVIADD47 pKa = 3.86KK48 pKa = 11.2QDD50 pKa = 3.14FFYY53 pKa = 11.06QEE55 pKa = 3.95YY56 pKa = 10.6GDD58 pKa = 4.49TYY60 pKa = 11.43DD61 pKa = 3.57SLIARR66 pKa = 11.84GVEE69 pKa = 3.96VVVAAEE75 pKa = 4.16TTDD78 pKa = 3.43PSVAHH83 pKa = 7.02PFTGQGAGDD92 pKa = 4.57GIVTPDD98 pKa = 3.01IALADD103 pKa = 3.88VQAEE107 pKa = 4.3NYY109 pKa = 9.82SAIAFVGGWGASMYY123 pKa = 10.5QYY125 pKa = 11.24AFPGDD130 pKa = 4.17YY131 pKa = 11.26ANDD134 pKa = 3.8AYY136 pKa = 10.82DD137 pKa = 4.01GNEE140 pKa = 3.7ATQTIVNNLIGDD152 pKa = 4.99FIDD155 pKa = 3.22QDD157 pKa = 3.7KK158 pKa = 10.76YY159 pKa = 10.87VAAVCNGVTVLAWARR174 pKa = 11.84VDD176 pKa = 3.83GVSPLAGRR184 pKa = 11.84TAAVPYY190 pKa = 9.61IGSPAATYY198 pKa = 9.6MGIEE202 pKa = 4.08YY203 pKa = 10.66GNYY206 pKa = 8.98GWAQPMQMDD215 pKa = 4.36ANGINYY221 pKa = 8.38FPSGAIGVADD231 pKa = 5.04DD232 pKa = 4.71PSDD235 pKa = 4.07DD236 pKa = 3.51VWVDD240 pKa = 3.35GQFITAEE247 pKa = 4.27NNHH250 pKa = 5.89SAAEE254 pKa = 4.06FGDD257 pKa = 4.17VIATHH262 pKa = 6.72VINAAVEE269 pKa = 4.24EE270 pKa = 4.42VEE272 pKa = 4.85EE273 pKa = 4.38VPEE276 pKa = 4.07NQAPIVSPGNLVIAEE291 pKa = 4.07NSVAGTVVGQVIASDD306 pKa = 3.72SDD308 pKa = 3.52LGQLLTYY315 pKa = 10.42AIIGGNDD322 pKa = 2.82SGAFTIEE329 pKa = 3.85AATGVITVANPAALDD344 pKa = 4.01FEE346 pKa = 4.82ASPLFEE352 pKa = 4.47LTVQVTDD359 pKa = 3.63NGTPSLNGVASVMIDD374 pKa = 3.51LLDD377 pKa = 4.04EE378 pKa = 5.17LEE380 pKa = 4.48TPPGPVSHH388 pKa = 7.72DD389 pKa = 3.56GPNVLVQGTADD400 pKa = 3.98ADD402 pKa = 4.11TIYY405 pKa = 10.19IWSDD409 pKa = 3.49YY410 pKa = 10.77YY411 pKa = 11.13GQQFVWINGLQTGPLALGSGGRR433 pKa = 11.84VIVFGGDD440 pKa = 3.3GNDD443 pKa = 3.07QVYY446 pKa = 7.97ATDD449 pKa = 3.37SAKK452 pKa = 10.47PVTIYY457 pKa = 11.32GEE459 pKa = 4.54GGHH462 pKa = 7.27DD463 pKa = 3.72SLTGGSANDD472 pKa = 3.83VIDD475 pKa = 5.14GGEE478 pKa = 3.87GWDD481 pKa = 4.49RR482 pKa = 11.84IWAGGGDD489 pKa = 3.96DD490 pKa = 5.76LIVGGAGTDD499 pKa = 3.24WLHH502 pKa = 5.99GRR504 pKa = 11.84EE505 pKa = 4.32GNDD508 pKa = 4.22LIVGGDD514 pKa = 3.65GDD516 pKa = 4.27DD517 pKa = 3.66YY518 pKa = 11.68LYY520 pKa = 11.26GFTGDD525 pKa = 4.11DD526 pKa = 3.66VLIGGQGSDD535 pKa = 3.48RR536 pKa = 11.84IEE538 pKa = 4.46GEE540 pKa = 3.94SGEE543 pKa = 4.49DD544 pKa = 3.44LLIGGTTSYY553 pKa = 11.43DD554 pKa = 3.68SNQLALEE561 pKa = 5.03AILADD566 pKa = 3.53WTAGADD572 pKa = 3.46LAGRR576 pKa = 11.84SGVFQLTFNGQTTADD591 pKa = 4.4DD592 pKa = 3.8SAQDD596 pKa = 3.8VLIGGADD603 pKa = 3.78NDD605 pKa = 3.94WLLIHH610 pKa = 7.07ASDD613 pKa = 4.37YY614 pKa = 10.7VHH616 pKa = 7.32ASQAGDD622 pKa = 3.66LLDD625 pKa = 3.63VLL627 pKa = 4.93

Molecular weight: 65.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517YDL6|A0A517YDL6_9BACT SHD1 domain-containing protein OS=Anatilimnocola aggregata OX=2528021 GN=ETAA8_34200 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84FDD4 pKa = 3.4AARR7 pKa = 11.84GRR9 pKa = 11.84FGRR12 pKa = 11.84TTKK15 pKa = 10.75DD16 pKa = 3.21RR17 pKa = 11.84LEE19 pKa = 4.38SAADD23 pKa = 3.23QWLSPWCWPPCQMIAAISCWVEE45 pKa = 3.52HH46 pKa = 6.35CFCIVLKK53 pKa = 10.75RR54 pKa = 11.84RR55 pKa = 11.84APLFADD61 pKa = 3.84EE62 pKa = 5.24CARR65 pKa = 11.84QLLAEE70 pKa = 4.25QASKK74 pKa = 10.8RR75 pKa = 11.84ADD77 pKa = 3.54CLSGQRR83 pKa = 11.84WFVRR87 pKa = 11.84VEE89 pKa = 3.77YY90 pKa = 10.67RR91 pKa = 11.84PDD93 pKa = 3.47LQTSAARR100 pKa = 11.84CWRR103 pKa = 11.84VRR105 pKa = 11.84KK106 pKa = 9.53HH107 pKa = 6.44VDD109 pKa = 3.86GISNQFHH116 pKa = 6.7SIQRR120 pKa = 11.84TVFPWKK126 pKa = 10.43FIIDD130 pKa = 4.16LQYY133 pKa = 10.51FAYY136 pKa = 10.72LSGFSLRR143 pKa = 11.84RR144 pKa = 11.84LPARR148 pKa = 11.84IRR150 pKa = 11.84PNSLFIARR158 pKa = 11.84LTRR161 pKa = 11.84RR162 pKa = 11.84SIRR165 pKa = 11.84QPQSCLCEE173 pKa = 4.44STSEE177 pKa = 4.15TSPARR182 pKa = 11.84PWRR185 pKa = 11.84FRR187 pKa = 11.84KK188 pKa = 10.19NSVGSTAPSWFFSQGKK204 pKa = 9.13

Molecular weight: 23.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6977

0

6977

2600830

29

7263

372.8

40.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.522 ± 0.039

1.096 ± 0.014

5.477 ± 0.024

5.839 ± 0.034

3.647 ± 0.016

7.907 ± 0.052

2.165 ± 0.019

4.719 ± 0.023

4.186 ± 0.036

10.285 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.976 ± 0.013

3.314 ± 0.035

5.562 ± 0.03

4.273 ± 0.025

6.35 ± 0.041

5.929 ± 0.027

5.763 ± 0.055

7.072 ± 0.024

1.545 ± 0.016

2.374 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski