Amino acid dipepetide frequency for Escherichia phage sortsyn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.675AlaAla: 13.675 ± 2.339
0.382AlaCys: 0.382 ± 0.171
7.105AlaAsp: 7.105 ± 0.881
7.181AlaGlu: 7.181 ± 0.742
3.132AlaPhe: 3.132 ± 0.569
7.945AlaGly: 7.945 ± 0.976
2.063AlaHis: 2.063 ± 0.432
6.341AlaIle: 6.341 ± 0.697
5.042AlaLys: 5.042 ± 0.587
9.244AlaLeu: 9.244 ± 0.964
2.445AlaMet: 2.445 ± 0.4
4.354AlaAsn: 4.354 ± 0.759
4.049AlaPro: 4.049 ± 0.637
5.959AlaGln: 5.959 ± 1.12
7.487AlaArg: 7.487 ± 0.965
5.5AlaSer: 5.5 ± 0.769
6.646AlaThr: 6.646 ± 0.704
6.035AlaVal: 6.035 ± 0.796
1.222AlaTrp: 1.222 ± 0.315
3.056AlaTyr: 3.056 ± 0.465
0.0AlaXaa: 0.0 ± 0.0
Cys
1.07CysAla: 1.07 ± 0.344
0.229CysCys: 0.229 ± 0.139
0.535CysAsp: 0.535 ± 0.239
0.84CysGlu: 0.84 ± 0.306
0.382CysPhe: 0.382 ± 0.212
1.222CysGly: 1.222 ± 0.368
0.382CysHis: 0.382 ± 0.178
0.229CysIle: 0.229 ± 0.138
0.688CysLys: 0.688 ± 0.318
0.688CysLeu: 0.688 ± 0.201
0.229CysMet: 0.229 ± 0.122
0.229CysAsn: 0.229 ± 0.119
0.382CysPro: 0.382 ± 0.166
0.076CysGln: 0.076 ± 0.079
1.528CysArg: 1.528 ± 0.447
0.229CysSer: 0.229 ± 0.124
0.306CysThr: 0.306 ± 0.188
0.688CysVal: 0.688 ± 0.236
0.076CysTrp: 0.076 ± 0.072
0.306CysTyr: 0.306 ± 0.159
0.0CysXaa: 0.0 ± 0.0
Asp
6.875AspAla: 6.875 ± 0.891
0.535AspCys: 0.535 ± 0.21
3.82AspAsp: 3.82 ± 0.548
5.118AspGlu: 5.118 ± 0.743
1.604AspPhe: 1.604 ± 0.422
5.653AspGly: 5.653 ± 0.742
1.528AspHis: 1.528 ± 0.396
2.597AspIle: 2.597 ± 0.504
3.056AspLys: 3.056 ± 0.612
6.188AspLeu: 6.188 ± 0.632
1.299AspMet: 1.299 ± 0.33
2.827AspAsn: 2.827 ± 0.517
4.202AspPro: 4.202 ± 0.558
3.667AspGln: 3.667 ± 0.532
3.591AspArg: 3.591 ± 0.645
2.75AspSer: 2.75 ± 0.605
2.674AspThr: 2.674 ± 0.356
4.66AspVal: 4.66 ± 0.687
2.215AspTrp: 2.215 ± 0.47
1.604AspTyr: 1.604 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
9.091GluAla: 9.091 ± 1.021
0.993GluCys: 0.993 ± 0.289
4.354GluAsp: 4.354 ± 0.725
3.82GluGlu: 3.82 ± 0.576
2.063GluPhe: 2.063 ± 0.416
3.591GluGly: 3.591 ± 0.423
0.917GluHis: 0.917 ± 0.333
3.667GluIle: 3.667 ± 0.595
4.736GluLys: 4.736 ± 0.552
5.042GluLeu: 5.042 ± 0.637
2.445GluMet: 2.445 ± 0.438
2.292GluAsn: 2.292 ± 0.442
3.132GluPro: 3.132 ± 0.485
2.979GluGln: 2.979 ± 0.434
4.202GluArg: 4.202 ± 0.597
3.591GluSer: 3.591 ± 0.51
3.056GluThr: 3.056 ± 0.432
3.361GluVal: 3.361 ± 0.66
1.604GluTrp: 1.604 ± 0.356
1.757GluTyr: 1.757 ± 0.295
0.0GluXaa: 0.0 ± 0.0
Phe
2.827PheAla: 2.827 ± 0.464
0.229PheCys: 0.229 ± 0.128
1.528PheAsp: 1.528 ± 0.361
2.063PheGlu: 2.063 ± 0.394
0.993PhePhe: 0.993 ± 0.345
2.979PheGly: 2.979 ± 0.439
0.535PheHis: 0.535 ± 0.197
1.375PheIle: 1.375 ± 0.334
1.681PheLys: 1.681 ± 0.372
2.674PheLeu: 2.674 ± 0.41
1.07PheMet: 1.07 ± 0.33
1.604PheAsn: 1.604 ± 0.352
1.146PhePro: 1.146 ± 0.298
1.07PheGln: 1.07 ± 0.291
2.215PheArg: 2.215 ± 0.306
1.986PheSer: 1.986 ± 0.322
0.993PheThr: 0.993 ± 0.247
1.986PheVal: 1.986 ± 0.304
1.07PheTrp: 1.07 ± 0.312
0.917PheTyr: 0.917 ± 0.381
0.0PheXaa: 0.0 ± 0.0
Gly
6.799GlyAla: 6.799 ± 0.804
0.458GlyCys: 0.458 ± 0.173
4.813GlyAsp: 4.813 ± 0.688
5.195GlyGlu: 5.195 ± 0.673
2.445GlyPhe: 2.445 ± 0.505
7.563GlyGly: 7.563 ± 1.131
1.146GlyHis: 1.146 ± 0.318
4.431GlyIle: 4.431 ± 0.576
4.202GlyLys: 4.202 ± 0.671
4.584GlyLeu: 4.584 ± 0.656
2.215GlyMet: 2.215 ± 0.455
2.292GlyAsn: 2.292 ± 0.397
2.903GlyPro: 2.903 ± 0.467
3.285GlyGln: 3.285 ± 0.623
4.966GlyArg: 4.966 ± 0.629
4.507GlySer: 4.507 ± 0.545
4.889GlyThr: 4.889 ± 0.981
5.271GlyVal: 5.271 ± 0.961
1.528GlyTrp: 1.528 ± 0.394
2.903GlyTyr: 2.903 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
1.451HisAla: 1.451 ± 0.289
0.458HisCys: 0.458 ± 0.178
1.299HisAsp: 1.299 ± 0.295
1.528HisGlu: 1.528 ± 0.324
0.611HisPhe: 0.611 ± 0.276
0.458HisGly: 0.458 ± 0.184
0.688HisHis: 0.688 ± 0.264
1.07HisIle: 1.07 ± 0.223
0.993HisLys: 0.993 ± 0.264
1.375HisLeu: 1.375 ± 0.366
0.458HisMet: 0.458 ± 0.226
0.611HisAsn: 0.611 ± 0.18
1.375HisPro: 1.375 ± 0.353
0.84HisGln: 0.84 ± 0.247
0.993HisArg: 0.993 ± 0.305
0.764HisSer: 0.764 ± 0.209
1.146HisThr: 1.146 ± 0.336
0.764HisVal: 0.764 ± 0.236
0.535HisTrp: 0.535 ± 0.204
0.611HisTyr: 0.611 ± 0.235
0.0HisXaa: 0.0 ± 0.0
Ile
5.5IleAla: 5.5 ± 0.668
0.611IleCys: 0.611 ± 0.227
3.667IleAsp: 3.667 ± 0.516
3.896IleGlu: 3.896 ± 0.458
1.299IlePhe: 1.299 ± 0.299
3.514IleGly: 3.514 ± 0.537
0.917IleHis: 0.917 ± 0.271
2.368IleIle: 2.368 ± 0.669
2.674IleLys: 2.674 ± 0.475
3.132IleLeu: 3.132 ± 0.644
1.833IleMet: 1.833 ± 0.325
1.91IleAsn: 1.91 ± 0.342
2.827IlePro: 2.827 ± 0.491
2.215IleGln: 2.215 ± 0.496
3.591IleArg: 3.591 ± 0.562
2.292IleSer: 2.292 ± 0.471
3.209IleThr: 3.209 ± 0.434
3.438IleVal: 3.438 ± 0.572
0.764IleTrp: 0.764 ± 0.239
1.528IleTyr: 1.528 ± 0.393
0.0IleXaa: 0.0 ± 0.0
Lys
6.417LysAla: 6.417 ± 0.737
0.382LysCys: 0.382 ± 0.195
4.354LysAsp: 4.354 ± 0.577
4.125LysGlu: 4.125 ± 0.522
1.222LysPhe: 1.222 ± 0.253
3.591LysGly: 3.591 ± 0.524
1.222LysHis: 1.222 ± 0.291
2.521LysIle: 2.521 ± 0.395
4.431LysLys: 4.431 ± 0.71
4.431LysLeu: 4.431 ± 0.757
2.063LysMet: 2.063 ± 0.361
2.139LysAsn: 2.139 ± 0.299
2.215LysPro: 2.215 ± 0.48
2.674LysGln: 2.674 ± 0.483
3.514LysArg: 3.514 ± 0.549
2.597LysSer: 2.597 ± 0.438
2.521LysThr: 2.521 ± 0.468
3.361LysVal: 3.361 ± 0.546
0.535LysTrp: 0.535 ± 0.189
2.139LysTyr: 2.139 ± 0.548
0.0LysXaa: 0.0 ± 0.0
Leu
9.015LeuAla: 9.015 ± 1.0
0.917LeuCys: 0.917 ± 0.244
5.5LeuAsp: 5.5 ± 0.72
4.431LeuGlu: 4.431 ± 0.654
2.063LeuPhe: 2.063 ± 0.363
5.195LeuGly: 5.195 ± 0.788
1.146LeuHis: 1.146 ± 0.253
4.507LeuIle: 4.507 ± 0.487
3.361LeuLys: 3.361 ± 0.588
6.035LeuLeu: 6.035 ± 0.694
1.986LeuMet: 1.986 ± 0.437
3.514LeuAsn: 3.514 ± 0.578
3.896LeuPro: 3.896 ± 0.504
2.674LeuGln: 2.674 ± 0.602
4.507LeuArg: 4.507 ± 0.707
4.049LeuSer: 4.049 ± 0.614
5.195LeuThr: 5.195 ± 0.526
5.424LeuVal: 5.424 ± 0.524
1.07LeuTrp: 1.07 ± 0.281
2.063LeuTyr: 2.063 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
4.125MetAla: 4.125 ± 0.544
0.229MetCys: 0.229 ± 0.126
1.681MetAsp: 1.681 ± 0.502
1.681MetGlu: 1.681 ± 0.403
1.222MetPhe: 1.222 ± 0.279
1.146MetGly: 1.146 ± 0.26
0.382MetHis: 0.382 ± 0.199
2.597MetIle: 2.597 ± 0.426
1.299MetLys: 1.299 ± 0.279
1.222MetLeu: 1.222 ± 0.228
1.07MetMet: 1.07 ± 0.282
1.146MetAsn: 1.146 ± 0.204
1.604MetPro: 1.604 ± 0.335
1.299MetGln: 1.299 ± 0.314
1.833MetArg: 1.833 ± 0.433
1.222MetSer: 1.222 ± 0.327
1.528MetThr: 1.528 ± 0.279
1.681MetVal: 1.681 ± 0.429
0.306MetTrp: 0.306 ± 0.132
0.764MetTyr: 0.764 ± 0.242
0.0MetXaa: 0.0 ± 0.0
Asn
4.736AsnAla: 4.736 ± 0.625
0.306AsnCys: 0.306 ± 0.185
2.139AsnAsp: 2.139 ± 0.35
1.299AsnGlu: 1.299 ± 0.328
0.917AsnPhe: 0.917 ± 0.269
4.813AsnGly: 4.813 ± 0.636
0.917AsnHis: 0.917 ± 0.23
1.986AsnIle: 1.986 ± 0.426
2.674AsnLys: 2.674 ± 0.369
3.209AsnLeu: 3.209 ± 0.478
1.07AsnMet: 1.07 ± 0.307
1.757AsnAsn: 1.757 ± 0.334
2.674AsnPro: 2.674 ± 0.461
1.146AsnGln: 1.146 ± 0.375
2.215AsnArg: 2.215 ± 0.443
2.674AsnSer: 2.674 ± 0.465
2.139AsnThr: 2.139 ± 0.483
2.521AsnVal: 2.521 ± 0.663
0.764AsnTrp: 0.764 ± 0.231
0.917AsnTyr: 0.917 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
5.348ProAla: 5.348 ± 0.642
0.764ProCys: 0.764 ± 0.295
4.813ProAsp: 4.813 ± 0.581
4.354ProGlu: 4.354 ± 0.607
1.91ProPhe: 1.91 ± 0.276
3.591ProGly: 3.591 ± 0.557
0.84ProHis: 0.84 ± 0.261
1.986ProIle: 1.986 ± 0.376
1.986ProLys: 1.986 ± 0.356
3.438ProLeu: 3.438 ± 0.671
0.764ProMet: 0.764 ± 0.244
1.222ProAsn: 1.222 ± 0.251
1.986ProPro: 1.986 ± 0.422
1.91ProGln: 1.91 ± 0.543
1.757ProArg: 1.757 ± 0.398
1.986ProSer: 1.986 ± 0.449
3.514ProThr: 3.514 ± 0.524
2.75ProVal: 2.75 ± 0.518
0.535ProTrp: 0.535 ± 0.167
1.91ProTyr: 1.91 ± 0.512
0.0ProXaa: 0.0 ± 0.0
Gln
5.424GlnAla: 5.424 ± 1.287
0.306GlnCys: 0.306 ± 0.184
2.521GlnAsp: 2.521 ± 0.356
3.056GlnGlu: 3.056 ± 0.727
1.528GlnPhe: 1.528 ± 0.418
2.75GlnGly: 2.75 ± 0.586
0.611GlnHis: 0.611 ± 0.231
1.757GlnIle: 1.757 ± 0.423
2.903GlnLys: 2.903 ± 0.46
3.667GlnLeu: 3.667 ± 0.733
1.375GlnMet: 1.375 ± 0.32
2.139GlnAsn: 2.139 ± 0.442
1.986GlnPro: 1.986 ± 0.486
5.042GlnGln: 5.042 ± 1.068
3.209GlnArg: 3.209 ± 0.607
1.528GlnSer: 1.528 ± 0.394
2.521GlnThr: 2.521 ± 0.426
2.368GlnVal: 2.368 ± 0.43
0.688GlnTrp: 0.688 ± 0.213
0.688GlnTyr: 0.688 ± 0.271
0.0GlnXaa: 0.0 ± 0.0
Arg
5.73ArgAla: 5.73 ± 0.667
0.764ArgCys: 0.764 ± 0.353
4.202ArgAsp: 4.202 ± 0.499
4.354ArgGlu: 4.354 ± 0.577
2.368ArgPhe: 2.368 ± 0.49
4.354ArgGly: 4.354 ± 0.404
1.07ArgHis: 1.07 ± 0.338
3.667ArgIle: 3.667 ± 0.55
4.431ArgLys: 4.431 ± 0.815
5.195ArgLeu: 5.195 ± 0.856
1.451ArgMet: 1.451 ± 0.299
3.209ArgAsn: 3.209 ± 0.515
2.139ArgPro: 2.139 ± 0.479
3.285ArgGln: 3.285 ± 0.383
3.438ArgArg: 3.438 ± 0.495
3.209ArgSer: 3.209 ± 0.394
2.979ArgThr: 2.979 ± 0.424
3.514ArgVal: 3.514 ± 0.584
0.764ArgTrp: 0.764 ± 0.236
1.91ArgTyr: 1.91 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
3.896SerAla: 3.896 ± 0.457
0.306SerCys: 0.306 ± 0.147
3.667SerAsp: 3.667 ± 0.556
2.979SerGlu: 2.979 ± 0.561
2.063SerPhe: 2.063 ± 0.41
4.736SerGly: 4.736 ± 0.775
0.84SerHis: 0.84 ± 0.415
1.91SerIle: 1.91 ± 0.381
2.979SerLys: 2.979 ± 0.398
3.285SerLeu: 3.285 ± 0.519
1.528SerMet: 1.528 ± 0.296
2.521SerAsn: 2.521 ± 0.572
1.986SerPro: 1.986 ± 0.294
2.139SerGln: 2.139 ± 0.471
3.667SerArg: 3.667 ± 0.504
3.361SerSer: 3.361 ± 1.01
3.209SerThr: 3.209 ± 0.471
3.972SerVal: 3.972 ± 0.496
0.382SerTrp: 0.382 ± 0.168
1.222SerTyr: 1.222 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
5.424ThrAla: 5.424 ± 0.707
0.458ThrCys: 0.458 ± 0.204
3.667ThrAsp: 3.667 ± 0.484
3.82ThrGlu: 3.82 ± 0.548
1.375ThrPhe: 1.375 ± 0.329
5.118ThrGly: 5.118 ± 0.658
0.764ThrHis: 0.764 ± 0.23
2.597ThrIle: 2.597 ± 0.448
3.667ThrLys: 3.667 ± 0.462
4.66ThrLeu: 4.66 ± 0.778
1.528ThrMet: 1.528 ± 0.306
2.521ThrAsn: 2.521 ± 0.576
4.431ThrPro: 4.431 ± 0.651
2.139ThrGln: 2.139 ± 0.478
2.75ThrArg: 2.75 ± 0.453
2.674ThrSer: 2.674 ± 0.471
3.438ThrThr: 3.438 ± 0.593
3.056ThrVal: 3.056 ± 0.511
0.688ThrTrp: 0.688 ± 0.258
1.833ThrTyr: 1.833 ± 0.383
0.0ThrXaa: 0.0 ± 0.0
Val
6.799ValAla: 6.799 ± 0.743
1.222ValCys: 1.222 ± 0.394
3.514ValAsp: 3.514 ± 0.554
3.82ValGlu: 3.82 ± 0.519
1.91ValPhe: 1.91 ± 0.456
4.584ValGly: 4.584 ± 0.578
0.917ValHis: 0.917 ± 0.24
3.591ValIle: 3.591 ± 0.496
4.125ValLys: 4.125 ± 0.579
4.049ValLeu: 4.049 ± 0.563
1.986ValMet: 1.986 ± 0.368
2.521ValAsn: 2.521 ± 0.46
2.75ValPro: 2.75 ± 0.374
2.292ValGln: 2.292 ± 0.575
3.743ValArg: 3.743 ± 0.656
3.132ValSer: 3.132 ± 0.533
3.591ValThr: 3.591 ± 0.586
3.438ValVal: 3.438 ± 0.488
1.07ValTrp: 1.07 ± 0.366
1.91ValTyr: 1.91 ± 0.348
0.0ValXaa: 0.0 ± 0.0
Trp
1.299TrpAla: 1.299 ± 0.295
0.306TrpCys: 0.306 ± 0.14
1.299TrpAsp: 1.299 ± 0.292
1.222TrpGlu: 1.222 ± 0.299
0.764TrpPhe: 0.764 ± 0.28
1.451TrpGly: 1.451 ± 0.329
0.535TrpHis: 0.535 ± 0.234
0.917TrpIle: 0.917 ± 0.218
0.535TrpLys: 0.535 ± 0.219
1.528TrpLeu: 1.528 ± 0.317
0.382TrpMet: 0.382 ± 0.185
1.07TrpAsn: 1.07 ± 0.274
0.458TrpPro: 0.458 ± 0.16
0.611TrpGln: 0.611 ± 0.244
1.146TrpArg: 1.146 ± 0.263
0.688TrpSer: 0.688 ± 0.309
1.146TrpThr: 1.146 ± 0.331
0.535TrpVal: 0.535 ± 0.185
0.153TrpTrp: 0.153 ± 0.112
0.229TrpTyr: 0.229 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.361TyrAla: 3.361 ± 0.644
0.458TyrCys: 0.458 ± 0.214
1.986TyrAsp: 1.986 ± 0.394
1.91TyrGlu: 1.91 ± 0.461
1.07TyrPhe: 1.07 ± 0.31
2.139TyrGly: 2.139 ± 0.41
0.611TyrHis: 0.611 ± 0.221
1.07TyrIle: 1.07 ± 0.291
0.993TyrLys: 0.993 ± 0.341
2.903TyrLeu: 2.903 ± 0.385
0.764TyrMet: 0.764 ± 0.241
0.993TyrAsn: 0.993 ± 0.316
1.222TyrPro: 1.222 ± 0.385
0.84TyrGln: 0.84 ± 0.238
1.681TyrArg: 1.681 ± 0.346
1.91TyrSer: 1.91 ± 0.393
1.91TyrThr: 1.91 ± 0.355
2.139TyrVal: 2.139 ± 0.393
0.306TyrTrp: 0.306 ± 0.153
0.993TyrTyr: 0.993 ± 0.234
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13091 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski