Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Sulfolobus; Sulfolobus acidocaldarius

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4JBV8|Q4JBV8_SULAC Selenium-binding protein OS=Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) OX=330779 GN=Saci_0305 PE=3 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84EE3 pKa = 4.81DD4 pKa = 3.87FDD6 pKa = 3.96QLLFGLAGVIFLEE19 pKa = 4.36GLGIALDD26 pKa = 3.82VVGQYY31 pKa = 11.19AIGSVLIVIATVWILSIYY49 pKa = 10.49FFMKK53 pKa = 10.61YY54 pKa = 9.41IEE56 pKa = 4.9KK57 pKa = 10.03KK58 pKa = 10.31DD59 pKa = 3.66SEE61 pKa = 4.3NEE63 pKa = 3.91EE64 pKa = 4.41NEE66 pKa = 4.13DD67 pKa = 3.18NSYY70 pKa = 11.88NNFF73 pKa = 3.36

Molecular weight: 8.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O05639|RL19E_SULAC 50S ribosomal protein L19e OS=Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) OX=330779 GN=rpl19e PE=3 SV=1
MM1 pKa = 7.55GKK3 pKa = 9.87YY4 pKa = 9.65KK5 pKa = 10.57PPAEE9 pKa = 3.93RR10 pKa = 11.84RR11 pKa = 11.84FGKK14 pKa = 10.37GVQLCKK20 pKa = 10.31RR21 pKa = 11.84CGSRR25 pKa = 11.84DD26 pKa = 3.64SVIQKK31 pKa = 10.35YY32 pKa = 9.95GLYY35 pKa = 10.4LCRR38 pKa = 11.84QCFRR42 pKa = 11.84EE43 pKa = 3.88VAYY46 pKa = 10.04PMGFRR51 pKa = 11.84KK52 pKa = 7.99TRR54 pKa = 3.11

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2222

0

2222

631988

32

1452

284.4

32.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.571 ± 0.052

0.581 ± 0.014

4.859 ± 0.04

6.81 ± 0.056

4.28 ± 0.042

6.712 ± 0.048

1.287 ± 0.02

9.1 ± 0.06

7.467 ± 0.057

10.245 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.023

4.822 ± 0.045

3.927 ± 0.035

2.277 ± 0.028

4.575 ± 0.045

6.998 ± 0.055

4.888 ± 0.046

7.797 ± 0.047

0.988 ± 0.018

4.627 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski