Amino acid dipepetide frequency for Gordonia phage Gsput1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.932AlaAla: 13.932 ± 1.515
1.416AlaCys: 1.416 ± 0.416
8.345AlaAsp: 8.345 ± 0.815
7.301AlaGlu: 7.301 ± 0.801
3.502AlaPhe: 3.502 ± 0.505
9.537AlaGly: 9.537 ± 1.73
2.086AlaHis: 2.086 ± 0.426
4.694AlaIle: 4.694 ± 0.625
5.662AlaLys: 5.662 ± 0.72
9.686AlaLeu: 9.686 ± 0.883
2.384AlaMet: 2.384 ± 0.494
4.023AlaAsn: 4.023 ± 0.469
3.949AlaPro: 3.949 ± 0.531
4.619AlaGln: 4.619 ± 0.732
8.568AlaArg: 8.568 ± 1.025
6.705AlaSer: 6.705 ± 1.071
7.525AlaThr: 7.525 ± 1.132
10.356AlaVal: 10.356 ± 0.815
2.98AlaTrp: 2.98 ± 0.434
2.831AlaTyr: 2.831 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
1.043CysAla: 1.043 ± 0.251
0.0CysCys: 0.0 ± 0.0
0.671CysAsp: 0.671 ± 0.229
0.82CysGlu: 0.82 ± 0.194
0.149CysPhe: 0.149 ± 0.105
0.894CysGly: 0.894 ± 0.309
0.447CysHis: 0.447 ± 0.176
0.373CysIle: 0.373 ± 0.156
0.373CysLys: 0.373 ± 0.162
0.298CysLeu: 0.298 ± 0.163
0.075CysMet: 0.075 ± 0.073
0.373CysAsn: 0.373 ± 0.169
0.82CysPro: 0.82 ± 0.269
0.224CysGln: 0.224 ± 0.115
0.745CysArg: 0.745 ± 0.273
0.298CysSer: 0.298 ± 0.14
0.298CysThr: 0.298 ± 0.141
0.373CysVal: 0.373 ± 0.163
0.373CysTrp: 0.373 ± 0.177
0.224CysTyr: 0.224 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
8.121AspAla: 8.121 ± 0.749
0.522AspCys: 0.522 ± 0.132
5.364AspAsp: 5.364 ± 0.634
6.482AspGlu: 6.482 ± 0.831
2.161AspPhe: 2.161 ± 0.384
7.003AspGly: 7.003 ± 0.588
1.49AspHis: 1.49 ± 0.315
1.341AspIle: 1.341 ± 0.304
1.788AspLys: 1.788 ± 0.404
5.513AspLeu: 5.513 ± 0.619
1.49AspMet: 1.49 ± 0.295
1.49AspAsn: 1.49 ± 0.385
3.204AspPro: 3.204 ± 0.392
2.533AspGln: 2.533 ± 0.369
3.8AspArg: 3.8 ± 0.498
3.353AspSer: 3.353 ± 0.578
2.608AspThr: 2.608 ± 0.503
6.035AspVal: 6.035 ± 0.568
1.118AspTrp: 1.118 ± 0.274
2.31AspTyr: 2.31 ± 0.44
0.0AspXaa: 0.0 ± 0.0
Glu
8.195GluAla: 8.195 ± 0.97
0.596GluCys: 0.596 ± 0.203
3.725GluAsp: 3.725 ± 0.662
1.714GluGlu: 1.714 ± 0.43
2.757GluPhe: 2.757 ± 0.413
5.066GluGly: 5.066 ± 0.67
1.639GluHis: 1.639 ± 0.368
2.682GluIle: 2.682 ± 0.442
1.416GluLys: 1.416 ± 0.402
6.035GluLeu: 6.035 ± 0.79
1.118GluMet: 1.118 ± 0.317
1.788GluAsn: 1.788 ± 0.459
2.384GluPro: 2.384 ± 0.405
2.086GluGln: 2.086 ± 0.419
5.811GluArg: 5.811 ± 0.691
3.502GluSer: 3.502 ± 0.526
2.533GluThr: 2.533 ± 0.422
4.843GluVal: 4.843 ± 0.692
1.639GluTrp: 1.639 ± 0.263
2.31GluTyr: 2.31 ± 0.458
0.0GluXaa: 0.0 ± 0.0
Phe
4.098PheAla: 4.098 ± 0.586
0.149PheCys: 0.149 ± 0.088
2.384PheAsp: 2.384 ± 0.443
2.682PheGlu: 2.682 ± 0.379
0.671PhePhe: 0.671 ± 0.205
3.725PheGly: 3.725 ± 0.644
0.447PheHis: 0.447 ± 0.17
1.192PheIle: 1.192 ± 0.298
1.416PheLys: 1.416 ± 0.34
0.969PheLeu: 0.969 ± 0.302
0.522PheMet: 0.522 ± 0.161
0.373PheAsn: 0.373 ± 0.156
0.671PhePro: 0.671 ± 0.168
0.596PheGln: 0.596 ± 0.229
2.235PheArg: 2.235 ± 0.405
1.043PheSer: 1.043 ± 0.288
2.757PheThr: 2.757 ± 0.383
2.235PheVal: 2.235 ± 0.376
0.373PheTrp: 0.373 ± 0.148
0.596PheTyr: 0.596 ± 0.227
0.0PheXaa: 0.0 ± 0.0
Gly
11.623GlyAla: 11.623 ± 1.228
0.298GlyCys: 0.298 ± 0.137
7.897GlyAsp: 7.897 ± 0.715
5.439GlyGlu: 5.439 ± 0.52
2.608GlyPhe: 2.608 ± 0.404
10.878GlyGly: 10.878 ± 1.468
1.714GlyHis: 1.714 ± 0.401
3.8GlyIle: 3.8 ± 0.484
4.917GlyLys: 4.917 ± 0.541
6.258GlyLeu: 6.258 ± 0.896
2.086GlyMet: 2.086 ± 0.396
3.353GlyAsn: 3.353 ± 0.565
4.098GlyPro: 4.098 ± 0.766
2.757GlyGln: 2.757 ± 0.337
6.556GlyArg: 6.556 ± 0.621
6.854GlySer: 6.854 ± 0.884
4.843GlyThr: 4.843 ± 0.649
7.972GlyVal: 7.972 ± 0.753
2.086GlyTrp: 2.086 ± 0.417
2.757GlyTyr: 2.757 ± 0.507
0.0GlyXaa: 0.0 ± 0.0
His
1.937HisAla: 1.937 ± 0.38
0.298HisCys: 0.298 ± 0.154
1.788HisAsp: 1.788 ± 0.409
1.565HisGlu: 1.565 ± 0.427
0.298HisPhe: 0.298 ± 0.147
2.831HisGly: 2.831 ± 0.441
0.447HisHis: 0.447 ± 0.163
0.447HisIle: 0.447 ± 0.144
0.82HisLys: 0.82 ± 0.25
1.043HisLeu: 1.043 ± 0.313
0.447HisMet: 0.447 ± 0.154
0.596HisAsn: 0.596 ± 0.199
0.894HisPro: 0.894 ± 0.266
0.224HisGln: 0.224 ± 0.126
1.416HisArg: 1.416 ± 0.366
0.894HisSer: 0.894 ± 0.228
0.969HisThr: 0.969 ± 0.295
2.161HisVal: 2.161 ± 0.408
0.596HisTrp: 0.596 ± 0.264
0.82HisTyr: 0.82 ± 0.344
0.0HisXaa: 0.0 ± 0.0
Ile
5.29IleAla: 5.29 ± 0.805
0.075IleCys: 0.075 ± 0.077
2.235IleAsp: 2.235 ± 0.377
3.949IleGlu: 3.949 ± 0.504
0.596IlePhe: 0.596 ± 0.192
4.023IleGly: 4.023 ± 0.604
0.596IleHis: 0.596 ± 0.231
0.894IleIle: 0.894 ± 0.228
0.671IleLys: 0.671 ± 0.22
1.788IleLeu: 1.788 ± 0.368
0.447IleMet: 0.447 ± 0.181
1.043IleAsn: 1.043 ± 0.251
1.788IlePro: 1.788 ± 0.389
0.522IleGln: 0.522 ± 0.167
2.012IleArg: 2.012 ± 0.33
1.788IleSer: 1.788 ± 0.405
2.161IleThr: 2.161 ± 0.383
4.619IleVal: 4.619 ± 0.488
0.298IleTrp: 0.298 ± 0.156
0.373IleTyr: 0.373 ± 0.155
0.0IleXaa: 0.0 ± 0.0
Lys
6.705LysAla: 6.705 ± 1.0
0.149LysCys: 0.149 ± 0.111
2.533LysAsp: 2.533 ± 0.413
0.969LysGlu: 0.969 ± 0.297
0.596LysPhe: 0.596 ± 0.222
4.172LysGly: 4.172 ± 0.52
0.745LysHis: 0.745 ± 0.212
0.82LysIle: 0.82 ± 0.235
0.671LysLys: 0.671 ± 0.209
3.129LysLeu: 3.129 ± 0.494
0.671LysMet: 0.671 ± 0.272
1.49LysAsn: 1.49 ± 0.343
2.012LysPro: 2.012 ± 0.429
0.969LysGln: 0.969 ± 0.232
2.533LysArg: 2.533 ± 0.545
2.31LysSer: 2.31 ± 0.404
1.714LysThr: 1.714 ± 0.367
4.023LysVal: 4.023 ± 0.57
0.671LysTrp: 0.671 ± 0.214
1.267LysTyr: 1.267 ± 0.317
0.0LysXaa: 0.0 ± 0.0
Leu
9.835LeuAla: 9.835 ± 0.781
0.969LeuCys: 0.969 ± 0.267
6.929LeuAsp: 6.929 ± 0.714
2.98LeuGlu: 2.98 ± 0.517
2.012LeuPhe: 2.012 ± 0.474
7.301LeuGly: 7.301 ± 0.802
0.969LeuHis: 0.969 ± 0.267
2.682LeuIle: 2.682 ± 0.456
3.129LeuLys: 3.129 ± 0.534
5.141LeuLeu: 5.141 ± 0.616
1.416LeuMet: 1.416 ± 0.305
2.384LeuAsn: 2.384 ± 0.481
4.247LeuPro: 4.247 ± 0.574
2.235LeuGln: 2.235 ± 0.367
5.886LeuArg: 5.886 ± 0.607
3.949LeuSer: 3.949 ± 0.67
5.066LeuThr: 5.066 ± 0.576
6.184LeuVal: 6.184 ± 0.612
1.118LeuTrp: 1.118 ± 0.296
1.788LeuTyr: 1.788 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
2.161MetAla: 2.161 ± 0.406
0.075MetCys: 0.075 ± 0.063
0.894MetAsp: 0.894 ± 0.238
0.298MetGlu: 0.298 ± 0.122
0.298MetPhe: 0.298 ± 0.166
1.937MetGly: 1.937 ± 0.345
0.0MetHis: 0.0 ± 0.0
0.522MetIle: 0.522 ± 0.218
1.043MetLys: 1.043 ± 0.25
1.639MetLeu: 1.639 ± 0.27
0.149MetMet: 0.149 ± 0.089
0.82MetAsn: 0.82 ± 0.302
0.82MetPro: 0.82 ± 0.237
0.596MetGln: 0.596 ± 0.254
1.639MetArg: 1.639 ± 0.358
1.714MetSer: 1.714 ± 0.379
2.384MetThr: 2.384 ± 0.42
1.565MetVal: 1.565 ± 0.324
0.596MetTrp: 0.596 ± 0.174
0.298MetTyr: 0.298 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
4.098AsnAla: 4.098 ± 0.556
0.149AsnCys: 0.149 ± 0.112
1.341AsnAsp: 1.341 ± 0.346
1.49AsnGlu: 1.49 ± 0.395
0.671AsnPhe: 0.671 ± 0.174
5.066AsnGly: 5.066 ± 0.663
0.596AsnHis: 0.596 ± 0.165
0.82AsnIle: 0.82 ± 0.246
1.118AsnLys: 1.118 ± 0.253
2.31AsnLeu: 2.31 ± 0.508
0.671AsnMet: 0.671 ± 0.19
0.671AsnAsn: 0.671 ± 0.219
1.863AsnPro: 1.863 ± 0.308
1.267AsnGln: 1.267 ± 0.23
1.714AsnArg: 1.714 ± 0.321
1.341AsnSer: 1.341 ± 0.351
1.714AsnThr: 1.714 ± 0.403
2.161AsnVal: 2.161 ± 0.373
0.373AsnTrp: 0.373 ± 0.158
0.671AsnTyr: 0.671 ± 0.227
0.0AsnXaa: 0.0 ± 0.0
Pro
3.353ProAla: 3.353 ± 0.568
0.447ProCys: 0.447 ± 0.182
3.427ProAsp: 3.427 ± 0.585
3.874ProGlu: 3.874 ± 0.586
1.341ProPhe: 1.341 ± 0.343
4.023ProGly: 4.023 ± 0.567
1.192ProHis: 1.192 ± 0.338
1.565ProIle: 1.565 ± 0.344
1.788ProLys: 1.788 ± 0.4
3.576ProLeu: 3.576 ± 0.424
0.671ProMet: 0.671 ± 0.225
1.565ProAsn: 1.565 ± 0.374
1.49ProPro: 1.49 ± 0.43
1.863ProGln: 1.863 ± 0.434
2.533ProArg: 2.533 ± 0.35
1.863ProSer: 1.863 ± 0.346
2.831ProThr: 2.831 ± 0.409
3.651ProVal: 3.651 ± 0.476
1.341ProTrp: 1.341 ± 0.36
0.745ProTyr: 0.745 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
3.651GlnAla: 3.651 ± 0.484
0.224GlnCys: 0.224 ± 0.127
0.894GlnAsp: 0.894 ± 0.287
0.671GlnGlu: 0.671 ± 0.207
0.969GlnPhe: 0.969 ± 0.262
2.31GlnGly: 2.31 ± 0.45
0.373GlnHis: 0.373 ± 0.159
1.49GlnIle: 1.49 ± 0.309
0.894GlnLys: 0.894 ± 0.316
3.204GlnLeu: 3.204 ± 0.553
0.447GlnMet: 0.447 ± 0.156
0.671GlnAsn: 0.671 ± 0.211
1.565GlnPro: 1.565 ± 0.303
0.745GlnGln: 0.745 ± 0.251
3.055GlnArg: 3.055 ± 0.508
2.31GlnSer: 2.31 ± 0.382
2.235GlnThr: 2.235 ± 0.441
3.427GlnVal: 3.427 ± 0.481
0.969GlnTrp: 0.969 ± 0.194
0.82GlnTyr: 0.82 ± 0.22
0.0GlnXaa: 0.0 ± 0.0
Arg
8.195ArgAla: 8.195 ± 0.939
0.745ArgCys: 0.745 ± 0.316
5.141ArgAsp: 5.141 ± 0.614
5.439ArgGlu: 5.439 ± 0.776
2.608ArgPhe: 2.608 ± 0.49
5.811ArgGly: 5.811 ± 0.699
2.161ArgHis: 2.161 ± 0.431
2.757ArgIle: 2.757 ± 0.485
3.204ArgLys: 3.204 ± 0.519
4.917ArgLeu: 4.917 ± 0.547
2.086ArgMet: 2.086 ± 0.449
2.31ArgAsn: 2.31 ± 0.391
2.757ArgPro: 2.757 ± 0.473
2.086ArgGln: 2.086 ± 0.347
6.109ArgArg: 6.109 ± 0.832
3.204ArgSer: 3.204 ± 0.516
3.204ArgThr: 3.204 ± 0.488
7.227ArgVal: 7.227 ± 0.618
1.49ArgTrp: 1.49 ± 0.316
2.235ArgTyr: 2.235 ± 0.415
0.0ArgXaa: 0.0 ± 0.0
Ser
7.748SerAla: 7.748 ± 0.926
0.298SerCys: 0.298 ± 0.136
3.502SerAsp: 3.502 ± 0.528
2.608SerGlu: 2.608 ± 0.366
1.863SerPhe: 1.863 ± 0.344
7.897SerGly: 7.897 ± 1.265
0.82SerHis: 0.82 ± 0.291
1.639SerIle: 1.639 ± 0.368
1.341SerLys: 1.341 ± 0.277
4.768SerLeu: 4.768 ± 0.76
1.192SerMet: 1.192 ± 0.254
1.416SerAsn: 1.416 ± 0.42
1.788SerPro: 1.788 ± 0.341
1.788SerGln: 1.788 ± 0.397
4.768SerArg: 4.768 ± 0.635
3.353SerSer: 3.353 ± 0.556
2.31SerThr: 2.31 ± 0.472
4.619SerVal: 4.619 ± 0.557
1.043SerTrp: 1.043 ± 0.291
0.969SerTyr: 0.969 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
5.96ThrAla: 5.96 ± 0.807
0.522ThrCys: 0.522 ± 0.205
3.502ThrAsp: 3.502 ± 0.644
2.608ThrGlu: 2.608 ± 0.46
1.937ThrPhe: 1.937 ± 0.348
5.439ThrGly: 5.439 ± 0.84
1.341ThrHis: 1.341 ± 0.346
2.757ThrIle: 2.757 ± 0.452
2.98ThrLys: 2.98 ± 0.625
4.768ThrLeu: 4.768 ± 0.559
0.969ThrMet: 0.969 ± 0.29
1.416ThrAsn: 1.416 ± 0.317
2.757ThrPro: 2.757 ± 0.394
1.341ThrGln: 1.341 ± 0.353
3.576ThrArg: 3.576 ± 0.467
2.757ThrSer: 2.757 ± 0.607
2.98ThrThr: 2.98 ± 0.601
5.513ThrVal: 5.513 ± 0.658
1.118ThrTrp: 1.118 ± 0.276
1.863ThrTyr: 1.863 ± 0.512
0.0ThrXaa: 0.0 ± 0.0
Val
9.835ValAla: 9.835 ± 0.908
1.416ValCys: 1.416 ± 0.452
4.396ValAsp: 4.396 ± 0.501
7.45ValGlu: 7.45 ± 0.869
2.459ValPhe: 2.459 ± 0.432
7.003ValGly: 7.003 ± 0.628
2.012ValHis: 2.012 ± 0.43
3.129ValIle: 3.129 ± 0.455
3.8ValLys: 3.8 ± 0.632
6.258ValLeu: 6.258 ± 0.667
1.714ValMet: 1.714 ± 0.369
2.459ValAsn: 2.459 ± 0.338
4.172ValPro: 4.172 ± 0.573
2.608ValGln: 2.608 ± 0.375
6.407ValArg: 6.407 ± 0.71
5.588ValSer: 5.588 ± 0.643
6.184ValThr: 6.184 ± 0.697
7.003ValVal: 7.003 ± 0.776
1.863ValTrp: 1.863 ± 0.36
1.863ValTyr: 1.863 ± 0.43
0.0ValXaa: 0.0 ± 0.0
Trp
2.012TrpAla: 2.012 ± 0.418
0.075TrpCys: 0.075 ± 0.069
1.341TrpAsp: 1.341 ± 0.327
1.192TrpGlu: 1.192 ± 0.265
0.522TrpPhe: 0.522 ± 0.251
1.192TrpGly: 1.192 ± 0.38
0.447TrpHis: 0.447 ± 0.234
0.894TrpIle: 0.894 ± 0.25
0.671TrpLys: 0.671 ± 0.243
2.98TrpLeu: 2.98 ± 0.43
0.298TrpMet: 0.298 ± 0.13
1.043TrpAsn: 1.043 ± 0.359
0.82TrpPro: 0.82 ± 0.316
1.416TrpGln: 1.416 ± 0.286
1.714TrpArg: 1.714 ± 0.388
1.565TrpSer: 1.565 ± 0.395
0.596TrpThr: 0.596 ± 0.197
1.416TrpVal: 1.416 ± 0.35
0.447TrpTrp: 0.447 ± 0.189
0.373TrpTyr: 0.373 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.31TyrAla: 2.31 ± 0.374
0.447TyrCys: 0.447 ± 0.217
1.416TyrAsp: 1.416 ± 0.399
2.31TyrGlu: 2.31 ± 0.382
1.118TyrPhe: 1.118 ± 0.382
2.682TyrGly: 2.682 ± 0.478
0.969TyrHis: 0.969 ± 0.305
0.671TyrIle: 0.671 ± 0.184
0.447TyrLys: 0.447 ± 0.16
1.937TyrLeu: 1.937 ± 0.371
0.522TyrMet: 0.522 ± 0.2
0.82TyrAsn: 0.82 ± 0.218
1.043TyrPro: 1.043 ± 0.238
0.447TyrGln: 0.447 ± 0.219
2.608TyrArg: 2.608 ± 0.514
1.416TyrSer: 1.416 ± 0.377
1.192TyrThr: 1.192 ± 0.268
2.235TyrVal: 2.235 ± 0.428
0.522TyrTrp: 0.522 ± 0.195
0.671TyrTyr: 0.671 ± 0.272
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (13423 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski