Amino acid dipepetide frequency for Smithella sp. F21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.995AlaAla: 8.995 ± 0.206
1.252AlaCys: 1.252 ± 0.063
4.854AlaAsp: 4.854 ± 0.116
5.388AlaGlu: 5.388 ± 0.135
3.369AlaPhe: 3.369 ± 0.112
7.353AlaGly: 7.353 ± 0.151
1.538AlaHis: 1.538 ± 0.069
5.898AlaIle: 5.898 ± 0.129
5.083AlaLys: 5.083 ± 0.126
8.824AlaLeu: 8.824 ± 0.166
2.726AlaMet: 2.726 ± 0.096
2.63AlaAsn: 2.63 ± 0.078
2.683AlaPro: 2.683 ± 0.091
2.95AlaGln: 2.95 ± 0.087
4.755AlaArg: 4.755 ± 0.119
4.44AlaSer: 4.44 ± 0.119
3.831AlaThr: 3.831 ± 0.103
6.536AlaVal: 6.536 ± 0.139
0.814AlaTrp: 0.814 ± 0.052
2.576AlaTyr: 2.576 ± 0.076
0.0AlaXaa: 0.0 ± 0.0
Cys
0.945CysAla: 0.945 ± 0.054
0.23CysCys: 0.23 ± 0.025
0.593CysAsp: 0.593 ± 0.038
0.619CysGlu: 0.619 ± 0.046
0.529CysPhe: 0.529 ± 0.04
1.239CysGly: 1.239 ± 0.066
0.286CysHis: 0.286 ± 0.029
0.753CysIle: 0.753 ± 0.054
0.571CysLys: 0.571 ± 0.037
1.145CysLeu: 1.145 ± 0.055
0.294CysMet: 0.294 ± 0.027
0.392CysAsn: 0.392 ± 0.034
0.673CysPro: 0.673 ± 0.041
0.339CysGln: 0.339 ± 0.032
0.844CysArg: 0.844 ± 0.053
0.71CysSer: 0.71 ± 0.047
0.534CysThr: 0.534 ± 0.038
0.806CysVal: 0.806 ± 0.046
0.085CysTrp: 0.085 ± 0.015
0.406CysTyr: 0.406 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
4.491AspAla: 4.491 ± 0.102
0.643AspCys: 0.643 ± 0.042
2.865AspAsp: 2.865 ± 0.098
3.853AspGlu: 3.853 ± 0.102
2.579AspPhe: 2.579 ± 0.09
3.61AspGly: 3.61 ± 0.105
1.231AspHis: 1.231 ± 0.056
4.627AspIle: 4.627 ± 0.107
3.391AspLys: 3.391 ± 0.116
5.388AspLeu: 5.388 ± 0.124
1.508AspMet: 1.508 ± 0.07
1.722AspAsn: 1.722 ± 0.068
2.293AspPro: 2.293 ± 0.097
1.599AspGln: 1.599 ± 0.065
2.822AspArg: 2.822 ± 0.102
2.317AspSer: 2.317 ± 0.084
2.267AspThr: 2.267 ± 0.074
4.144AspVal: 4.144 ± 0.093
0.63AspTrp: 0.63 ± 0.041
2.136AspTyr: 2.136 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.636GluAla: 5.636 ± 0.135
0.515GluCys: 0.515 ± 0.037
3.132GluAsp: 3.132 ± 0.098
4.616GluGlu: 4.616 ± 0.144
2.229GluPhe: 2.229 ± 0.08
4.13GluGly: 4.13 ± 0.123
1.169GluHis: 1.169 ± 0.048
5.217GluIle: 5.217 ± 0.125
6.162GluLys: 6.162 ± 0.146
5.708GluLeu: 5.708 ± 0.134
1.946GluMet: 1.946 ± 0.072
2.966GluAsn: 2.966 ± 0.088
1.989GluPro: 1.989 ± 0.072
2.072GluGln: 2.072 ± 0.076
3.543GluArg: 3.543 ± 0.114
3.02GluSer: 3.02 ± 0.088
3.233GluThr: 3.233 ± 0.093
3.791GluVal: 3.791 ± 0.111
0.555GluTrp: 0.555 ± 0.038
1.797GluTyr: 1.797 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.449PheAla: 3.449 ± 0.095
0.646PheCys: 0.646 ± 0.033
2.462PheAsp: 2.462 ± 0.08
2.387PheGlu: 2.387 ± 0.079
2.24PhePhe: 2.24 ± 0.102
3.201PheGly: 3.201 ± 0.095
0.854PheHis: 0.854 ± 0.049
3.07PheIle: 3.07 ± 0.09
2.227PheLys: 2.227 ± 0.081
4.194PheLeu: 4.194 ± 0.117
1.111PheMet: 1.111 ± 0.052
1.591PheAsn: 1.591 ± 0.069
1.834PhePro: 1.834 ± 0.071
1.386PheGln: 1.386 ± 0.052
2.029PheArg: 2.029 ± 0.074
3.001PheSer: 3.001 ± 0.107
2.171PheThr: 2.171 ± 0.078
2.827PheVal: 2.827 ± 0.095
0.497PheTrp: 0.497 ± 0.037
1.468PheTyr: 1.468 ± 0.067
0.0PheXaa: 0.0 ± 0.0
Gly
6.007GlyAla: 6.007 ± 0.14
1.129GlyCys: 1.129 ± 0.068
3.626GlyAsp: 3.626 ± 0.121
4.053GlyGlu: 4.053 ± 0.111
3.356GlyPhe: 3.356 ± 0.101
5.425GlyGly: 5.425 ± 0.156
1.546GlyHis: 1.546 ± 0.065
5.879GlyIle: 5.879 ± 0.147
5.764GlyLys: 5.764 ± 0.11
7.179GlyLeu: 7.179 ± 0.143
2.483GlyMet: 2.483 ± 0.075
2.574GlyAsn: 2.574 ± 0.084
2.042GlyPro: 2.042 ± 0.077
2.205GlyGln: 2.205 ± 0.083
4.277GlyArg: 4.277 ± 0.107
4.141GlySer: 4.141 ± 0.11
3.748GlyThr: 3.748 ± 0.115
5.003GlyVal: 5.003 ± 0.119
0.825GlyTrp: 0.825 ± 0.045
2.606GlyTyr: 2.606 ± 0.097
0.0GlyXaa: 0.0 ± 0.0
His
1.492HisAla: 1.492 ± 0.061
0.278HisCys: 0.278 ± 0.026
0.958HisAsp: 0.958 ± 0.051
1.095HisGlu: 1.095 ± 0.05
0.993HisPhe: 0.993 ± 0.052
1.482HisGly: 1.482 ± 0.063
0.617HisHis: 0.617 ± 0.04
1.356HisIle: 1.356 ± 0.059
1.103HisLys: 1.103 ± 0.061
2.133HisLeu: 2.133 ± 0.074
0.398HisMet: 0.398 ± 0.032
0.673HisAsn: 0.673 ± 0.037
1.223HisPro: 1.223 ± 0.052
0.753HisGln: 0.753 ± 0.04
1.063HisArg: 1.063 ± 0.053
0.905HisSer: 0.905 ± 0.046
0.878HisThr: 0.878 ± 0.044
1.212HisVal: 1.212 ± 0.063
0.198HisTrp: 0.198 ± 0.023
0.78HisTyr: 0.78 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.733IleAla: 6.733 ± 0.149
0.788IleCys: 0.788 ± 0.053
4.266IleAsp: 4.266 ± 0.111
4.683IleGlu: 4.683 ± 0.125
3.265IlePhe: 3.265 ± 0.112
5.39IleGly: 5.39 ± 0.132
1.479IleHis: 1.479 ± 0.06
5.473IleIle: 5.473 ± 0.143
4.531IleLys: 4.531 ± 0.111
7.142IleLeu: 7.142 ± 0.141
1.73IleMet: 1.73 ± 0.063
2.945IleAsn: 2.945 ± 0.08
3.503IlePro: 3.503 ± 0.113
2.096IleGln: 2.096 ± 0.07
4.069IleArg: 4.069 ± 0.114
4.654IleSer: 4.654 ± 0.106
3.813IleThr: 3.813 ± 0.1
5.126IleVal: 5.126 ± 0.124
0.585IleTrp: 0.585 ± 0.042
2.147IleTyr: 2.147 ± 0.085
0.0IleXaa: 0.0 ± 0.0
Lys
5.292LysAla: 5.292 ± 0.134
0.526LysCys: 0.526 ± 0.044
3.626LysAsp: 3.626 ± 0.107
4.889LysGlu: 4.889 ± 0.136
2.096LysPhe: 2.096 ± 0.084
4.285LysGly: 4.285 ± 0.11
1.071LysHis: 1.071 ± 0.06
5.826LysIle: 5.826 ± 0.131
6.122LysLys: 6.122 ± 0.156
5.521LysLeu: 5.521 ± 0.136
2.355LysMet: 2.355 ± 0.085
3.068LysAsn: 3.068 ± 0.104
2.785LysPro: 2.785 ± 0.108
2.16LysGln: 2.16 ± 0.082
3.292LysArg: 3.292 ± 0.106
3.524LysSer: 3.524 ± 0.093
4.058LysThr: 4.058 ± 0.111
4.304LysVal: 4.304 ± 0.109
0.627LysTrp: 0.627 ± 0.044
2.088LysTyr: 2.088 ± 0.071
0.0LysXaa: 0.0 ± 0.0
Leu
8.899LeuAla: 8.899 ± 0.191
1.153LeuCys: 1.153 ± 0.057
5.126LeuAsp: 5.126 ± 0.118
5.786LeuGlu: 5.786 ± 0.135
4.098LeuPhe: 4.098 ± 0.119
6.632LeuGly: 6.632 ± 0.157
1.703LeuHis: 1.703 ± 0.069
6.677LeuIle: 6.677 ± 0.163
6.667LeuLys: 6.667 ± 0.12
9.286LeuLeu: 9.286 ± 0.218
2.667LeuMet: 2.667 ± 0.086
3.797LeuAsn: 3.797 ± 0.102
4.622LeuPro: 4.622 ± 0.119
3.164LeuGln: 3.164 ± 0.105
4.907LeuArg: 4.907 ± 0.127
6.659LeuSer: 6.659 ± 0.133
5.26LeuThr: 5.26 ± 0.125
5.994LeuVal: 5.994 ± 0.144
0.916LeuTrp: 0.916 ± 0.052
2.761LeuTyr: 2.761 ± 0.09
0.0LeuXaa: 0.0 ± 0.0
Met
2.766MetAla: 2.766 ± 0.092
0.246MetCys: 0.246 ± 0.026
1.661MetAsp: 1.661 ± 0.058
2.029MetGlu: 2.029 ± 0.084
0.886MetPhe: 0.886 ± 0.047
2.064MetGly: 2.064 ± 0.084
0.435MetHis: 0.435 ± 0.036
2.243MetIle: 2.243 ± 0.081
2.312MetLys: 2.312 ± 0.081
2.475MetLeu: 2.475 ± 0.089
0.798MetMet: 0.798 ± 0.053
1.271MetAsn: 1.271 ± 0.062
1.375MetPro: 1.375 ± 0.06
0.921MetGln: 0.921 ± 0.05
1.303MetArg: 1.303 ± 0.057
1.655MetSer: 1.655 ± 0.067
1.565MetThr: 1.565 ± 0.071
1.848MetVal: 1.848 ± 0.061
0.211MetTrp: 0.211 ± 0.022
0.585MetTyr: 0.585 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
2.99AsnAla: 2.99 ± 0.089
0.507AsnCys: 0.507 ± 0.036
1.917AsnAsp: 1.917 ± 0.064
2.069AsnGlu: 2.069 ± 0.067
1.615AsnPhe: 1.615 ± 0.062
2.424AsnGly: 2.424 ± 0.082
0.769AsnHis: 0.769 ± 0.043
3.343AsnIle: 3.343 ± 0.106
2.163AsnLys: 2.163 ± 0.079
3.724AsnLeu: 3.724 ± 0.103
1.172AsnMet: 1.172 ± 0.057
1.508AsnAsn: 1.508 ± 0.064
2.245AsnPro: 2.245 ± 0.076
1.071AsnGln: 1.071 ± 0.049
2.093AsnArg: 2.093 ± 0.063
1.703AsnSer: 1.703 ± 0.081
1.586AsnThr: 1.586 ± 0.066
2.6AsnVal: 2.6 ± 0.092
0.392AsnTrp: 0.392 ± 0.034
1.359AsnTyr: 1.359 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
3.842ProAla: 3.842 ± 0.114
0.395ProCys: 0.395 ± 0.032
2.849ProAsp: 2.849 ± 0.09
3.247ProGlu: 3.247 ± 0.091
1.853ProPhe: 1.853 ± 0.071
3.212ProGly: 3.212 ± 0.099
0.774ProHis: 0.774 ± 0.039
2.237ProIle: 2.237 ± 0.075
2.333ProLys: 2.333 ± 0.085
4.037ProLeu: 4.037 ± 0.107
1.033ProMet: 1.033 ± 0.05
1.263ProAsn: 1.263 ± 0.057
1.626ProPro: 1.626 ± 0.077
1.567ProGln: 1.567 ± 0.065
1.917ProArg: 1.917 ± 0.081
2.264ProSer: 2.264 ± 0.078
1.791ProThr: 1.791 ± 0.068
3.695ProVal: 3.695 ± 0.103
0.467ProTrp: 0.467 ± 0.037
1.383ProTyr: 1.383 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
2.953GlnAla: 2.953 ± 0.088
0.299GlnCys: 0.299 ± 0.032
1.65GlnAsp: 1.65 ± 0.064
1.981GlnGlu: 1.981 ± 0.073
1.14GlnPhe: 1.14 ± 0.057
2.176GlnGly: 2.176 ± 0.079
0.627GlnHis: 0.627 ± 0.038
2.456GlnIle: 2.456 ± 0.075
2.745GlnLys: 2.745 ± 0.098
2.689GlnLeu: 2.689 ± 0.074
1.116GlnMet: 1.116 ± 0.054
1.458GlnAsn: 1.458 ± 0.063
1.076GlnPro: 1.076 ± 0.052
1.089GlnGln: 1.089 ± 0.055
1.687GlnArg: 1.687 ± 0.068
1.781GlnSer: 1.781 ± 0.08
1.834GlnThr: 1.834 ± 0.075
2.008GlnVal: 2.008 ± 0.075
0.363GlnTrp: 0.363 ± 0.029
0.996GlnTyr: 0.996 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
3.938ArgAla: 3.938 ± 0.108
0.609ArgCys: 0.609 ± 0.044
2.889ArgAsp: 2.889 ± 0.095
4.058ArgGlu: 4.058 ± 0.105
2.374ArgPhe: 2.374 ± 0.083
3.522ArgGly: 3.522 ± 0.104
1.119ArgHis: 1.119 ± 0.056
3.943ArgIle: 3.943 ± 0.108
3.909ArgLys: 3.909 ± 0.087
5.503ArgLeu: 5.503 ± 0.122
1.597ArgMet: 1.597 ± 0.064
1.906ArgAsn: 1.906 ± 0.072
2.072ArgPro: 2.072 ± 0.067
2.171ArgGln: 2.171 ± 0.09
3.54ArgArg: 3.54 ± 0.106
2.681ArgSer: 2.681 ± 0.092
2.328ArgThr: 2.328 ± 0.069
3.377ArgVal: 3.377 ± 0.099
0.555ArgTrp: 0.555 ± 0.044
1.93ArgTyr: 1.93 ± 0.077
0.0ArgXaa: 0.0 ± 0.0
Ser
4.726SerAla: 4.726 ± 0.114
0.662SerCys: 0.662 ± 0.042
3.084SerAsp: 3.084 ± 0.094
3.257SerGlu: 3.257 ± 0.1
2.753SerPhe: 2.753 ± 0.09
4.923SerGly: 4.923 ± 0.135
1.164SerHis: 1.164 ± 0.053
3.826SerIle: 3.826 ± 0.094
2.806SerLys: 2.806 ± 0.091
5.754SerLeu: 5.754 ± 0.145
1.607SerMet: 1.607 ± 0.076
1.765SerAsn: 1.765 ± 0.069
2.475SerPro: 2.475 ± 0.077
1.666SerGln: 1.666 ± 0.071
3.287SerArg: 3.287 ± 0.102
3.289SerSer: 3.289 ± 0.117
2.555SerThr: 2.555 ± 0.078
3.842SerVal: 3.842 ± 0.095
0.603SerTrp: 0.603 ± 0.042
1.807SerTyr: 1.807 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
4.427ThrAla: 4.427 ± 0.117
0.579ThrCys: 0.579 ± 0.04
2.632ThrAsp: 2.632 ± 0.088
2.859ThrGlu: 2.859 ± 0.082
2.093ThrPhe: 2.093 ± 0.073
4.854ThrGly: 4.854 ± 0.131
0.993ThrHis: 0.993 ± 0.042
3.551ThrIle: 3.551 ± 0.091
2.753ThrLys: 2.753 ± 0.088
5.041ThrLeu: 5.041 ± 0.113
1.292ThrMet: 1.292 ± 0.058
1.567ThrAsn: 1.567 ± 0.074
2.507ThrPro: 2.507 ± 0.085
1.351ThrGln: 1.351 ± 0.064
2.398ThrArg: 2.398 ± 0.073
2.552ThrSer: 2.552 ± 0.088
2.502ThrThr: 2.502 ± 0.092
3.583ThrVal: 3.583 ± 0.1
0.531ThrTrp: 0.531 ± 0.037
1.447ThrTyr: 1.447 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
5.847ValAla: 5.847 ± 0.128
0.964ValCys: 0.964 ± 0.053
3.668ValAsp: 3.668 ± 0.098
4.085ValGlu: 4.085 ± 0.103
3.038ValPhe: 3.038 ± 0.115
4.734ValGly: 4.734 ± 0.118
1.239ValHis: 1.239 ± 0.056
5.324ValIle: 5.324 ± 0.119
4.598ValLys: 4.598 ± 0.115
6.584ValLeu: 6.584 ± 0.144
1.866ValMet: 1.866 ± 0.07
2.536ValAsn: 2.536 ± 0.081
2.777ValPro: 2.777 ± 0.079
2.0ValGln: 2.0 ± 0.075
3.49ValArg: 3.49 ± 0.104
4.261ValSer: 4.261 ± 0.106
3.575ValThr: 3.575 ± 0.107
5.27ValVal: 5.27 ± 0.144
0.654ValTrp: 0.654 ± 0.042
1.882ValTyr: 1.882 ± 0.074
0.0ValXaa: 0.0 ± 0.0
Trp
0.649TrpAla: 0.649 ± 0.04
0.101TrpCys: 0.101 ± 0.016
0.502TrpAsp: 0.502 ± 0.034
0.63TrpGlu: 0.63 ± 0.041
0.478TrpPhe: 0.478 ± 0.037
0.761TrpGly: 0.761 ± 0.056
0.251TrpHis: 0.251 ± 0.026
0.769TrpIle: 0.769 ± 0.045
0.651TrpLys: 0.651 ± 0.038
1.049TrpLeu: 1.049 ± 0.053
0.302TrpMet: 0.302 ± 0.032
0.416TrpAsn: 0.416 ± 0.034
0.443TrpPro: 0.443 ± 0.038
0.462TrpGln: 0.462 ± 0.04
0.662TrpArg: 0.662 ± 0.044
0.481TrpSer: 0.481 ± 0.041
0.462TrpThr: 0.462 ± 0.035
0.555TrpVal: 0.555 ± 0.038
0.136TrpTrp: 0.136 ± 0.018
0.27TrpTyr: 0.27 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.419TyrAla: 2.419 ± 0.072
0.47TyrCys: 0.47 ± 0.035
1.757TyrAsp: 1.757 ± 0.068
1.845TyrGlu: 1.845 ± 0.072
1.661TyrPhe: 1.661 ± 0.07
2.454TyrGly: 2.454 ± 0.082
0.724TyrHis: 0.724 ± 0.044
1.952TyrIle: 1.952 ± 0.079
1.623TyrLys: 1.623 ± 0.062
3.396TyrLeu: 3.396 ± 0.108
0.643TyrMet: 0.643 ± 0.041
1.204TyrAsn: 1.204 ± 0.074
1.543TyrPro: 1.543 ± 0.08
1.103TyrGln: 1.103 ± 0.05
2.042TyrArg: 2.042 ± 0.077
1.813TyrSer: 1.813 ± 0.077
1.522TyrThr: 1.522 ± 0.062
1.869TyrVal: 1.869 ± 0.067
0.384TyrTrp: 0.384 ± 0.029
1.124TyrTyr: 1.124 ± 0.071
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1346 proteins (374552 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski