Pisolithus microcarpus 441

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Sclerodermatineae; Pisolithaceae; Pisolithus; Pisolithus microcarpus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20748 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C9YU39|A0A0C9YU39_9AGAM Unplaced genomic scaffold scaffold_140 whole genome shotgun sequence OS=Pisolithus microcarpus 441 OX=765257 GN=PISMIDRAFT_221902 PE=4 SV=1
MM1 pKa = 7.89PPTTVYY7 pKa = 10.25RR8 pKa = 11.84RR9 pKa = 11.84QSMAQLYY16 pKa = 7.81EE17 pKa = 4.2KK18 pKa = 10.85LSAQSDD24 pKa = 4.07EE25 pKa = 6.56DD26 pKa = 4.02DD27 pKa = 4.2MAWLAQFSPEE37 pKa = 4.54DD38 pKa = 3.96KK39 pKa = 10.95SDD41 pKa = 3.74DD42 pKa = 3.7LYY44 pKa = 11.77APVAPSPNLSSITSCTSSSFPSAGPSPVDD73 pKa = 2.94GHH75 pKa = 8.08ADD77 pKa = 3.52DD78 pKa = 4.86TSSSDD83 pKa = 3.37EE84 pKa = 4.13SSSEE88 pKa = 4.2SEE90 pKa = 4.28SDD92 pKa = 3.68TEE94 pKa = 4.1PLSLTFDD101 pKa = 4.36FDD103 pKa = 3.8YY104 pKa = 8.47TTYY107 pKa = 10.75DD108 pKa = 3.24QSPCIPLIFQSDD120 pKa = 4.25DD121 pKa = 3.38LTNTTDD127 pKa = 3.53SPYY130 pKa = 10.61LPPVDD135 pKa = 3.97PTVAPQPTSVSLPGYY150 pKa = 6.89YY151 pKa = 10.1HH152 pKa = 7.4PAQPTLFHH160 pKa = 6.78GSSDD164 pKa = 3.98SPLLLPPTSPVVGSRR179 pKa = 11.84TVFKK183 pKa = 10.85GRR185 pKa = 11.84NASTNQADD193 pKa = 3.76RR194 pKa = 11.84RR195 pKa = 11.84DD196 pKa = 3.81DD197 pKa = 4.23SEE199 pKa = 4.11EE200 pKa = 3.85QTNEE204 pKa = 3.97EE205 pKa = 4.53SDD207 pKa = 4.54DD208 pKa = 5.04DD209 pKa = 4.77DD210 pKa = 7.12DD211 pKa = 5.59EE212 pKa = 4.43YY213 pKa = 11.9LPP215 pKa = 4.89

Molecular weight: 23.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C9Y1I2|A0A0C9Y1I2_9AGAM Unplaced genomic scaffold scaffold_636 whole genome shotgun sequence OS=Pisolithus microcarpus 441 OX=765257 GN=PISMIDRAFT_536663 PE=4 SV=1
FF1 pKa = 7.41RR2 pKa = 11.84QRR4 pKa = 11.84KK5 pKa = 8.83NNLTLIRR12 pKa = 11.84STTPRR17 pKa = 11.84KK18 pKa = 7.38TMKK21 pKa = 8.4WTWPSTSPTILQWKK35 pKa = 8.99KK36 pKa = 10.07GQPPRR41 pKa = 11.84NSRR44 pKa = 11.84KK45 pKa = 9.44RR46 pKa = 11.84RR47 pKa = 11.84SLFF50 pKa = 3.39

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20734

14

20748

5838774

49

5084

281.4

31.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.883 ± 0.016

1.72 ± 0.009

5.543 ± 0.012

5.79 ± 0.018

3.671 ± 0.013

6.196 ± 0.017

2.99 ± 0.011

4.827 ± 0.013

4.42 ± 0.018

9.396 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.007

3.391 ± 0.009

6.253 ± 0.022

3.877 ± 0.014

6.34 ± 0.017

8.611 ± 0.024

6.034 ± 0.014

6.569 ± 0.012

1.533 ± 0.008

2.72 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski