Frankia sp. EI5c

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; unclassified Frankia

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168IFP0|A0A168IFP0_9ACTN Uncharacterized protein OS=Frankia sp. EI5c OX=683316 GN=UG55_10923 PE=4 SV=1
VV1 pKa = 6.88PGGAIRR7 pKa = 11.84FTATYY12 pKa = 9.08TNTGQTPYY20 pKa = 11.26NNITIRR26 pKa = 11.84TSAADD31 pKa = 3.68LSDD34 pKa = 4.12DD35 pKa = 3.82FVGIGDD41 pKa = 3.64QTASSGVISIDD52 pKa = 3.14EE53 pKa = 4.2LTFEE57 pKa = 4.74SVWTGSIPVGGTVTLTGSGTVLDD80 pKa = 4.91PDD82 pKa = 4.15PGNRR86 pKa = 11.84DD87 pKa = 2.87IVTVVHH93 pKa = 5.67TTAXGSNCPVGNPAPACRR111 pKa = 11.84VSVPVLLPGLTMTTAADD128 pKa = 3.54VPTAVPGGVVNYY140 pKa = 8.27TVTITNSGEE149 pKa = 4.13TPYY152 pKa = 10.65TGAAVANNLTGALDD166 pKa = 3.77DD167 pKa = 4.19AVYY170 pKa = 10.67GGDD173 pKa = 3.32ATASTGSVAFAGQTLTWTGDD193 pKa = 3.42LGVGQVATVTYY204 pKa = 10.26SLTVRR209 pKa = 11.84NPDD212 pKa = 3.61PGDD215 pKa = 3.13KK216 pKa = 10.69VMRR219 pKa = 11.84NILSSTVQGSACPPNSGNTGCAGSVAVLTXALTIAKK255 pKa = 9.5SASTATXLPGEE266 pKa = 4.39AVTFTIAVSNTGQVPFPGATVVDD289 pKa = 3.92AXGGVLDD296 pKa = 4.42DD297 pKa = 3.72ATYY300 pKa = 11.18NGDD303 pKa = 3.54AGTTAGTVDD312 pKa = 3.51VTGSTLTWTGDD323 pKa = 3.7LNPGGSATVTYY334 pKa = 10.8SVTLLGNGLGSGDD347 pKa = 4.02GILANSATSXVAGNNCPTGGTDD369 pKa = 3.54PRR371 pKa = 11.84CSATVRR377 pKa = 11.84RR378 pKa = 11.84SEE380 pKa = 4.32LNISFTGNRR389 pKa = 11.84STAGPGEE396 pKa = 4.48VISRR400 pKa = 11.84IATITNTGQVPYY412 pKa = 9.97TGATVYY418 pKa = 10.75LSNDD422 pKa = 3.13DD423 pKa = 3.93TQNLLSYY430 pKa = 10.74NGDD433 pKa = 3.26ASTTTGSIQFDD444 pKa = 4.1LVNLRR449 pKa = 11.84IVWTGDD455 pKa = 3.38LAPXQAATITASSTVRR471 pKa = 11.84PVTQGGEE478 pKa = 4.24VTSVAEE484 pKa = 4.27SPTPGSNCPIGGSDD498 pKa = 3.67PACSNLLTVLKK509 pKa = 9.83PQLTIVKK516 pKa = 8.39TPSTTTTVPGGTITWTVTISNTGEE540 pKa = 3.85TAYY543 pKa = 9.76TGATVTDD550 pKa = 4.71DD551 pKa = 3.86LTGLAQDD558 pKa = 3.48TDD560 pKa = 4.28YY561 pKa = 11.84NGDD564 pKa = 3.33ATATTGALGYY574 pKa = 9.61SAPLLTWTGDD584 pKa = 3.77LAVGASATITFTTTVHH600 pKa = 5.17QQIQGPHH607 pKa = 5.96ALFNTVRR614 pKa = 11.84SAEE617 pKa = 4.24LGSTCPPSGGNAPGPCRR634 pKa = 11.84ALVLILIPQLTITKK648 pKa = 8.61TAATGSPAGTAVAXSPIDD666 pKa = 3.54YY667 pKa = 8.71TVTVANTGEE676 pKa = 4.21TPYY679 pKa = 10.21TGASFTDD686 pKa = 3.99DD687 pKa = 3.72LTDD690 pKa = 3.59VLDD693 pKa = 4.34DD694 pKa = 3.57AAYY697 pKa = 10.84NGDD700 pKa = 3.68AAXTTGTVDD709 pKa = 3.82FTDD712 pKa = 5.54PEE714 pKa = 4.49LTWTGDD720 pKa = 3.57LAIGATATITYY731 pKa = 10.08SVTTALPANGNHH743 pKa = 6.12TATNAVSSATPGTNCLAGGTDD764 pKa = 3.81TACTTTTTVLVPALSITKK782 pKa = 10.29AVDD785 pKa = 3.02QASIVTGGTVHH796 pKa = 5.36YY797 pKa = 8.8TITATNTGEE806 pKa = 4.19SPYY809 pKa = 11.0SGITITDD816 pKa = 3.7TLDD819 pKa = 3.89DD820 pKa = 4.17LAGNATWVGDD830 pKa = 3.73AAATTGTVDD839 pKa = 3.69FTDD842 pKa = 5.09PVLTWTGDD850 pKa = 3.62LAIXASVTITYY861 pKa = 10.37SVAVDD866 pKa = 3.73DD867 pKa = 4.54AAXXGAVLANRR878 pKa = 11.84VVSPAPGSTCTGLGTEE894 pKa = 4.75PACEE898 pKa = 3.71TSTTIDD904 pKa = 3.52AQTLTLTNLTDD915 pKa = 3.29SFTFSGQPQDD925 pKa = 5.06ALTLHH930 pKa = 6.62NAVTMTVITNSADD943 pKa = 3.24GYY945 pKa = 7.74TVSVQGTGPTLTAQNPANDD964 pKa = 3.69DD965 pKa = 4.26TIPLDD970 pKa = 3.76ALSVRR975 pKa = 11.84GTGQAGFQPLSEE987 pKa = 4.3TTPXLAHH994 pKa = 6.37SQDD997 pKa = 3.77APSAPGGDD1005 pKa = 4.04AISNDD1010 pKa = 3.49FSVDD1014 pKa = 3.14IPFVASDD1021 pKa = 4.1TYY1023 pKa = 11.05STTLDD1028 pKa = 3.5YY1029 pKa = 10.91IAATKK1034 pKa = 10.23

Molecular weight: 102.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166QV47|A0A166QV47_9ACTN Uncharacterized protein OS=Frankia sp. EI5c OX=683316 GN=UG55_103053 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84ARR5 pKa = 11.84VPAVARR11 pKa = 11.84ARR13 pKa = 11.84APVAVPLAVAPVAVAVPVPVAVAVAVAVVAATVVVAARR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 9.46RR54 pKa = 11.84RR55 pKa = 3.36

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5384

0

5384

1870666

22

7080

347.4

36.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.416 ± 0.051

0.766 ± 0.01

5.953 ± 0.026

5.185 ± 0.029

2.595 ± 0.017

9.988 ± 0.036

2.07 ± 0.014

3.261 ± 0.02

1.309 ± 0.02

10.158 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.542 ± 0.012

1.612 ± 0.016

6.802 ± 0.033

2.488 ± 0.017

8.736 ± 0.037

5.211 ± 0.022

5.95 ± 0.03

8.61 ± 0.032

1.397 ± 0.013

1.808 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski