Bovine polyomavirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097IQX9|A0A097IQX9_9POLY VP3 OS=Bovine polyomavirus 3 OX=1561705 PE=4 SV=1
MM1 pKa = 6.89ATCGAGRR8 pKa = 11.84GKK10 pKa = 9.05VTLPRR15 pKa = 11.84QIKK18 pKa = 10.05KK19 pKa = 10.72GGAEE23 pKa = 4.08VLDD26 pKa = 4.02TVPLSEE32 pKa = 4.14TTQYY36 pKa = 11.58AVEE39 pKa = 4.6FVNKK43 pKa = 8.67PTFLKK48 pKa = 10.4TNGDD52 pKa = 3.21PEE54 pKa = 4.14YY55 pKa = 10.47RR56 pKa = 11.84SRR58 pKa = 11.84GLQYY62 pKa = 11.31LLDD65 pKa = 4.92DD66 pKa = 4.21PTVGVQGALCYY77 pKa = 10.45SLGVIDD83 pKa = 6.32LPEE86 pKa = 5.16IPNAMCEE93 pKa = 4.01DD94 pKa = 3.94SVIVWEE100 pKa = 4.62AYY102 pKa = 9.68RR103 pKa = 11.84VEE105 pKa = 4.39TEE107 pKa = 5.17LITLPVLGSGGYY119 pKa = 9.66VSATKK124 pKa = 10.01TIASVEE130 pKa = 4.18GPQMYY135 pKa = 9.41FWAVGGSPLDD145 pKa = 3.48VVGFNPDD152 pKa = 3.2PDD154 pKa = 3.74GTYY157 pKa = 10.38TFAAEE162 pKa = 3.97LAAPKK167 pKa = 9.42GTISVAGPGATKK179 pKa = 10.59SQVTTPKK186 pKa = 9.44YY187 pKa = 9.13TVEE190 pKa = 3.77GWAADD195 pKa = 3.47PSMNDD200 pKa = 2.7NTKK203 pKa = 10.03YY204 pKa = 9.78FGRR207 pKa = 11.84IIGGSQTPPVITYY220 pKa = 10.38GNGSTTPLVDD230 pKa = 3.75EE231 pKa = 4.82YY232 pKa = 11.66GVGPLCLHH240 pKa = 6.21GVCYY244 pKa = 9.1LTSVDD249 pKa = 4.92LMGTVGWPGQATLPDD264 pKa = 3.71GGVKK268 pKa = 9.75KK269 pKa = 10.46RR270 pKa = 11.84ALQTAAGRR278 pKa = 11.84FFRR281 pKa = 11.84VHH283 pKa = 5.22FRR285 pKa = 11.84QRR287 pKa = 11.84RR288 pKa = 11.84VKK290 pKa = 10.76NPWTMEE296 pKa = 3.7QMLSQFLRR304 pKa = 11.84PKK306 pKa = 10.58APVVTGAQTEE316 pKa = 4.4VSEE319 pKa = 4.51VTMTQDD325 pKa = 3.84KK326 pKa = 10.44GPASIPPTLEE336 pKa = 3.55ASVGYY341 pKa = 9.44GQAIFNVVDD350 pKa = 4.05GQLVYY355 pKa = 10.59PDD357 pKa = 4.03GQGGSINIGTRR368 pKa = 11.84TQMM371 pKa = 3.74

Molecular weight: 39.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097IR00|A0A097IR00_9POLY VP2 OS=Bovine polyomavirus 3 OX=1561705 PE=4 SV=1
MM1 pKa = 7.76ALQLWFPQTGLWQNYY16 pKa = 7.8GVSLPDD22 pKa = 2.84WWSSLLSQLPDD33 pKa = 4.1PYY35 pKa = 10.87TILQDD40 pKa = 3.19IARR43 pKa = 11.84GIWTSYY49 pKa = 9.04YY50 pKa = 10.17RR51 pKa = 11.84AGQEE55 pKa = 3.58IVRR58 pKa = 11.84RR59 pKa = 11.84TVGNQIRR66 pKa = 11.84GFVGSVQEE74 pKa = 4.51NLLQTGRR81 pKa = 11.84AIVEE85 pKa = 4.11RR86 pKa = 11.84APDD89 pKa = 3.97PVTGLINVWNWATEE103 pKa = 3.91HH104 pKa = 4.96QRR106 pKa = 11.84QWEE109 pKa = 4.34TQALLEE115 pKa = 4.25GQPVFGRR122 pKa = 11.84EE123 pKa = 4.28TQWEE127 pKa = 4.46SQNLPIDD134 pKa = 3.84GGNNQRR140 pKa = 11.84DD141 pKa = 3.57GWHH144 pKa = 6.89WGGQWVTMPGSNLGAYY160 pKa = 7.4TVPQWMLYY168 pKa = 9.5VLEE171 pKa = 4.49EE172 pKa = 4.2VEE174 pKa = 4.54KK175 pKa = 11.14DD176 pKa = 3.28LTAHH180 pKa = 6.57HH181 pKa = 6.71GNMRR185 pKa = 11.84SRR187 pKa = 11.84KK188 pKa = 9.01RR189 pKa = 11.84QGDD192 pKa = 3.45PAKK195 pKa = 9.75TNKK198 pKa = 9.16KK199 pKa = 9.56RR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 3.59

Molecular weight: 23.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1756

188

673

351.2

39.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.403 ± 0.753

1.993 ± 0.637

4.613 ± 0.48

6.15 ± 0.576

3.417 ± 0.481

9.282 ± 1.099

1.936 ± 0.419

4.328 ± 0.275

5.41 ± 1.151

8.827 ± 0.564

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.205

4.271 ± 0.474

5.979 ± 0.413

5.125 ± 0.756

4.67 ± 0.698

5.011 ± 0.112

6.549 ± 0.819

6.891 ± 0.736

3.303 ± 0.78

2.563 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski