Xinzhou partiti-like virus 1
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLW6|A0A1L3KLW6_9VIRU RdRp OS=Xinzhou partiti-like virus 1 OX=1923776 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 10.44 RR4 pKa = 11.84 PAEE7 pKa = 4.09 PAFGTPAKK15 pKa = 10.79 VMAGDD20 pKa = 3.58 SADD23 pKa = 3.08 AHH25 pKa = 5.88 TEE27 pKa = 3.75 VSGVIGQDD35 pKa = 3.12 NPVPAPRR42 pKa = 11.84 RR43 pKa = 11.84 QSANKK48 pKa = 9.04 QQLLDD53 pKa = 3.81 SEE55 pKa = 4.71 LASRR59 pKa = 11.84 PKK61 pKa = 10.45 PEE63 pKa = 4.16 EE64 pKa = 3.71 LNILRR69 pKa = 11.84 TYY71 pKa = 10.23 ASNVIGPTPCHH82 pKa = 6.26 SDD84 pKa = 2.55 IYY86 pKa = 11.25 VLYY89 pKa = 9.49 THH91 pKa = 7.16 TALDD95 pKa = 3.71 AFAKK99 pKa = 10.11 LVRR102 pKa = 11.84 RR103 pKa = 11.84 QILNTLYY110 pKa = 10.13 PDD112 pKa = 4.6 GDD114 pKa = 4.07 FLPQGVCSEE123 pKa = 4.37 EE124 pKa = 3.89 DD125 pKa = 3.1 WVIVVQYY132 pKa = 10.01 ILKK135 pKa = 10.41 SRR137 pKa = 11.84 IDD139 pKa = 3.68 LVYY142 pKa = 10.81 ANYY145 pKa = 10.24 SGRR148 pKa = 11.84 RR149 pKa = 11.84 PPDD152 pKa = 3.1 RR153 pKa = 11.84 VPLVRR158 pKa = 11.84 IVMPRR163 pKa = 11.84 SIASLINGIGVKK175 pKa = 9.31 QVFHH179 pKa = 7.11 GNFSVCPQPGDD190 pKa = 3.68 PPVDD194 pKa = 3.26 RR195 pKa = 11.84 ATWLINLATHH205 pKa = 7.05 ARR207 pKa = 11.84 IQSFSNLVLAALQRR221 pKa = 11.84 GVIHH225 pKa = 7.31 PGTASNLVEE234 pKa = 4.17 GTGWWLLYY242 pKa = 10.25 AANSTNRR249 pKa = 11.84 GQIATIDD256 pKa = 3.97 TQSVTVWAQFPEE268 pKa = 4.36 WTPSDD273 pKa = 3.76 GLLCAIAATGFIGTFIDD290 pKa = 4.14 DD291 pKa = 3.58 WTEE294 pKa = 3.58 YY295 pKa = 9.47 TYY297 pKa = 11.49 KK298 pKa = 10.7 LEE300 pKa = 4.19 SVTDD304 pKa = 3.95 SVGMRR309 pKa = 11.84 ASYY312 pKa = 10.89 AVLGG316 pKa = 4.02
Molecular weight: 34.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.764
IPC2_protein 5.766
IPC_protein 5.804
Toseland 6.186
ProMoST 6.16
Dawson 6.084
Bjellqvist 6.071
Wikipedia 6.097
Rodwell 6.059
Grimsley 6.326
Solomon 6.084
Lehninger 6.071
Nozaki 6.338
DTASelect 6.532
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.44
Patrickios 4.024
IPC_peptide 6.097
IPC2_peptide 6.44
IPC2.peptide.svr19 6.382
Protein with the highest isoelectric point:
>tr|A0A1L3KLW6|A0A1L3KLW6_9VIRU RdRp OS=Xinzhou partiti-like virus 1 OX=1923776 PE=4 SV=1
MM1 pKa = 7.0 STLEE5 pKa = 4.42 FVRR8 pKa = 11.84 QLPSYY13 pKa = 11.39 SLMQHH18 pKa = 5.97 GTPCDD23 pKa = 3.51 LYY25 pKa = 11.57 ARR27 pKa = 11.84 GLINALYY34 pKa = 9.96 PEE36 pKa = 4.99 ALPSIDD42 pKa = 3.47 EE43 pKa = 4.35 YY44 pKa = 11.62 SRR46 pKa = 11.84 GMYY49 pKa = 10.07 DD50 pKa = 3.58 IEE52 pKa = 3.98 HH53 pKa = 7.33 HH54 pKa = 5.42 YY55 pKa = 8.88 TAFYY59 pKa = 10.09 KK60 pKa = 10.56 YY61 pKa = 10.54 SRR63 pKa = 11.84 PQYY66 pKa = 9.9 LEE68 pKa = 3.33 QAIKK72 pKa = 10.45 HH73 pKa = 5.56 RR74 pKa = 11.84 LEE76 pKa = 4.1 SSSLIKK82 pKa = 9.82 EE83 pKa = 3.51 ARR85 pKa = 11.84 AIVEE89 pKa = 4.3 SEE91 pKa = 3.9 LTEE94 pKa = 4.31 SFTVQAISMSHH105 pKa = 6.58 LDD107 pKa = 3.19 AVSFVGSSAAGYY119 pKa = 10.18 GYY121 pKa = 10.9 VGNKK125 pKa = 8.47 RR126 pKa = 11.84 DD127 pKa = 3.48 NYY129 pKa = 10.51 LVARR133 pKa = 11.84 GHH135 pKa = 5.91 ATSNLANFRR144 pKa = 11.84 RR145 pKa = 11.84 WGSKK149 pKa = 9.94 LRR151 pKa = 11.84 LTPYY155 pKa = 9.65 KK156 pKa = 10.42 AYY158 pKa = 10.92 SRR160 pKa = 11.84 TQLALRR166 pKa = 11.84 ADD168 pKa = 3.67 PKK170 pKa = 10.53 IRR172 pKa = 11.84 HH173 pKa = 4.48 VWGAPFHH180 pKa = 6.5 TILIEE185 pKa = 4.02 GTIAQPIIVNLQLYY199 pKa = 7.38 DD200 pKa = 3.5 QPIYY204 pKa = 10.2 IGKK207 pKa = 10.09 DD208 pKa = 3.05 MFKK211 pKa = 10.45 EE212 pKa = 3.93 LPYY215 pKa = 10.33 TLNRR219 pKa = 11.84 LLRR222 pKa = 11.84 DD223 pKa = 3.4 NFFAYY228 pKa = 10.55 CIDD231 pKa = 3.14 ISAFDD236 pKa = 3.58 SSVNQIFIDD245 pKa = 3.52 WFFDD249 pKa = 4.2 FVSKK253 pKa = 9.64 TVTFPNAFTRR263 pKa = 11.84 SAVDD267 pKa = 2.94 YY268 pKa = 11.15 CRR270 pKa = 11.84 FEE272 pKa = 5.0 LKK274 pKa = 8.8 HH275 pKa = 6.07 TPVVMPDD282 pKa = 2.38 GKK284 pKa = 10.46 MYY286 pKa = 10.16 ICHH289 pKa = 6.61 TGIPSGSYY297 pKa = 7.66 FTQLIDD303 pKa = 3.74 SYY305 pKa = 11.79 VNLIMLRR312 pKa = 11.84 ACQLSILEE320 pKa = 4.61 SVVPTYY326 pKa = 11.45 VLGDD330 pKa = 3.51 DD331 pKa = 5.96 SIFCHH336 pKa = 6.53 HH337 pKa = 6.73 NPNHH341 pKa = 6.28 LEE343 pKa = 3.91 SFRR346 pKa = 11.84 VLFEE350 pKa = 3.9 EE351 pKa = 5.81 FGFKK355 pKa = 10.91 LNVRR359 pKa = 11.84 KK360 pKa = 8.38 TIVSKK365 pKa = 10.82 SSQEE369 pKa = 3.83 IVFLGHH375 pKa = 6.17 NFYY378 pKa = 11.08 GSRR381 pKa = 11.84 LTRR384 pKa = 11.84 DD385 pKa = 4.05 DD386 pKa = 3.59 FTLACLAIHH395 pKa = 6.58 TEE397 pKa = 4.25 TPIVNSSQTVVRR409 pKa = 11.84 LASLLYY415 pKa = 10.61 DD416 pKa = 3.64 SGHH419 pKa = 6.14 NSFFLMNLLTAAIKK433 pKa = 10.27 RR434 pKa = 11.84 YY435 pKa = 9.18 GSPRR439 pKa = 11.84 PLHH442 pKa = 6.08 PPYY445 pKa = 10.65 VQLFMLSS452 pKa = 3.25
Molecular weight: 51.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.081
IPC2_protein 7.234
IPC_protein 7.22
Toseland 7.102
ProMoST 7.644
Dawson 7.834
Bjellqvist 8.068
Wikipedia 7.775
Rodwell 7.834
Grimsley 7.22
Solomon 7.892
Lehninger 7.907
Nozaki 8.17
DTASelect 7.98
Thurlkill 8.009
EMBOSS 8.083
Sillero 8.258
Patrickios 3.656
IPC_peptide 7.892
IPC2_peptide 7.468
IPC2.peptide.svr19 7.324
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
768
316
452
384.0
43.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.682 ± 1.133
1.432 ± 0.104
5.078 ± 0.387
3.906 ± 0.266
4.948 ± 1.313
5.599 ± 0.852
2.995 ± 0.685
6.51 ± 0.113
3.125 ± 0.371
9.766 ± 0.764
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.823 ± 0.348
4.036 ± 0.048
5.859 ± 0.888
3.776 ± 0.409
5.729 ± 0.021
8.464 ± 0.741
5.99 ± 0.41
7.031 ± 0.946
1.302 ± 0.571
4.948 ± 0.917
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here