Nocardioides gansuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4337 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T8FE27|A0A2T8FE27_9ACTN Uncharacterized protein OS=Nocardioides gansuensis OX=2138300 GN=DDE18_06725 PE=3 SV=1
MM1 pKa = 7.31YY2 pKa = 10.26PEE4 pKa = 4.41DD5 pKa = 3.46LKK7 pKa = 10.87YY8 pKa = 9.38TSEE11 pKa = 4.5HH12 pKa = 4.81EE13 pKa = 4.37WVRR16 pKa = 11.84QPGEE20 pKa = 3.92AEE22 pKa = 3.89GSVRR26 pKa = 11.84IGITHH31 pKa = 7.34FAQDD35 pKa = 3.54ALGDD39 pKa = 3.7IVYY42 pKa = 10.51VSLPEE47 pKa = 4.26VGTQVAAGEE56 pKa = 4.54SCGEE60 pKa = 4.09LEE62 pKa = 4.39STKK65 pKa = 10.62SVSDD69 pKa = 3.26IYY71 pKa = 11.4APVSGEE77 pKa = 3.8VVARR81 pKa = 11.84NEE83 pKa = 4.2ALDD86 pKa = 3.78ATPEE90 pKa = 4.07LVNDD94 pKa = 4.04DD95 pKa = 4.65PYY97 pKa = 11.67AGGWLFEE104 pKa = 4.35VVPSDD109 pKa = 3.26AAAVDD114 pKa = 3.9GLLDD118 pKa = 3.72AAAYY122 pKa = 9.1QAGLQAA128 pKa = 5.01

Molecular weight: 13.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T8F969|A0A2T8F969_9ACTN ATP-dependent helicase OS=Nocardioides gansuensis OX=2138300 GN=DDE18_12335 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84SSRR5 pKa = 11.84PRR7 pKa = 11.84APATRR12 pKa = 11.84QRR14 pKa = 11.84GRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84SRR21 pKa = 11.84ARR23 pKa = 11.84RR24 pKa = 11.84PARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84PRR31 pKa = 11.84PRR33 pKa = 11.84PPRR36 pKa = 11.84PMRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84PPRR44 pKa = 11.84VSRR47 pKa = 11.84HH48 pKa = 4.44TWPCATRR55 pKa = 11.84HH56 pKa = 4.94TRR58 pKa = 11.84PGG60 pKa = 3.15

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4337

0

4337

1410258

31

6671

325.2

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.982 ± 0.061

0.778 ± 0.011

6.403 ± 0.033

6.028 ± 0.034

2.83 ± 0.024

9.173 ± 0.033

2.334 ± 0.019

3.42 ± 0.024

1.932 ± 0.026

10.277 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.882 ± 0.017

1.723 ± 0.022

5.644 ± 0.026

2.784 ± 0.019

7.725 ± 0.045

5.277 ± 0.026

5.994 ± 0.039

9.329 ± 0.034

1.565 ± 0.015

1.918 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski