Amino acid dipepetide frequency for Methylophaga lonarensis MPL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.6AlaAla: 9.6 ± 0.137	1.032AlaCys: 1.032 ± 0.038	5.895AlaAsp: 5.895 ± 0.082	7.109AlaGlu: 7.109 ± 0.116	3.471AlaPhe: 3.471 ± 0.074	7.118AlaGly: 7.118 ± 0.113	1.917AlaHis: 1.917 ± 0.053	6.093AlaIle: 6.093 ± 0.088	3.455AlaLys: 3.455 ± 0.077	10.49AlaLeu: 10.49 ± 0.136	3.031AlaMet: 3.031 ± 0.062	3.236AlaAsn: 3.236 ± 0.071	3.03AlaPro: 3.03 ± 0.071	4.058AlaGln: 4.058 ± 0.077	5.08AlaArg: 5.08 ± 0.077	5.703AlaSer: 5.703 ± 0.083	4.274AlaThr: 4.274 ± 0.074	6.82AlaVal: 6.82 ± 0.098	1.055AlaTrp: 1.055 ± 0.039	2.235AlaTyr: 2.235 ± 0.06	0.001AlaXaa: 0.001 ± 0.001
Cys	0.713CysAla: 0.713 ± 0.028	0.158CysCys: 0.158 ± 0.014	0.566CysAsp: 0.566 ± 0.029	0.566CysGlu: 0.566 ± 0.026	0.357CysPhe: 0.357 ± 0.021	0.897CysGly: 0.897 ± 0.039	0.357CysHis: 0.357 ± 0.02	0.504CysIle: 0.504 ± 0.026	0.313CysLys: 0.313 ± 0.021	1.045CysLeu: 1.045 ± 0.036	0.224CysMet: 0.224 ± 0.017	0.275CysAsn: 0.275 ± 0.019	0.513CysPro: 0.513 ± 0.029	0.522CysGln: 0.522 ± 0.028	0.549CysArg: 0.549 ± 0.028	0.572CysSer: 0.572 ± 0.028	0.369CysThr: 0.369 ± 0.021	0.593CysVal: 0.593 ± 0.028	0.103CysTrp: 0.103 ± 0.012	0.274CysTyr: 0.274 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.161AspAla: 5.161 ± 0.081	0.552AspCys: 0.552 ± 0.031	3.314AspAsp: 3.314 ± 0.071	3.911AspGlu: 3.911 ± 0.079	2.508AspPhe: 2.508 ± 0.061	3.743AspGly: 3.743 ± 0.085	1.234AspHis: 1.234 ± 0.044	4.002AspIle: 4.002 ± 0.076	2.448AspLys: 2.448 ± 0.059	5.761AspLeu: 5.761 ± 0.096	1.536AspMet: 1.536 ± 0.044	2.274AspAsn: 2.274 ± 0.062	2.381AspPro: 2.381 ± 0.056	2.341AspGln: 2.341 ± 0.054	3.042AspArg: 3.042 ± 0.063	3.536AspSer: 3.536 ± 0.067	2.664AspThr: 2.664 ± 0.057	3.787AspVal: 3.787 ± 0.08	1.071AspTrp: 1.071 ± 0.047	1.926AspTyr: 1.926 ± 0.061	0.003AspXaa: 0.003 ± 0.002
Glu	6.165GluAla: 6.165 ± 0.107	0.421GluCys: 0.421 ± 0.024	2.819GluAsp: 2.819 ± 0.073	2.841GluGlu: 2.841 ± 0.082	2.209GluPhe: 2.209 ± 0.059	3.562GluGly: 3.562 ± 0.072	1.621GluHis: 1.621 ± 0.051	4.077GluIle: 4.077 ± 0.084	2.717GluLys: 2.717 ± 0.073	6.745GluLeu: 6.745 ± 0.109	1.825GluMet: 1.825 ± 0.054	2.241GluAsn: 2.241 ± 0.058	2.278GluPro: 2.278 ± 0.064	4.743GluGln: 4.743 ± 0.104	3.814GluArg: 3.814 ± 0.069	3.274GluSer: 3.274 ± 0.07	3.351GluThr: 3.351 ± 0.066	4.3GluVal: 4.3 ± 0.085	0.668GluTrp: 0.668 ± 0.027	1.478GluTyr: 1.478 ± 0.045	0.003GluXaa: 0.003 ± 0.002
Phe	3.707PheAla: 3.707 ± 0.067	0.477PheCys: 0.477 ± 0.023	2.623PheAsp: 2.623 ± 0.058	2.408PheGlu: 2.408 ± 0.05	1.654PhePhe: 1.654 ± 0.046	2.828PheGly: 2.828 ± 0.073	0.804PheHis: 0.804 ± 0.03	2.378PheIle: 2.378 ± 0.057	1.407PheLys: 1.407 ± 0.041	3.484PheLeu: 3.484 ± 0.075	0.995PheMet: 0.995 ± 0.033	1.656PheAsn: 1.656 ± 0.05	1.434PhePro: 1.434 ± 0.041	1.336PheGln: 1.336 ± 0.041	2.032PheArg: 2.032 ± 0.05	3.009PheSer: 3.009 ± 0.065	2.012PheThr: 2.012 ± 0.049	2.651PheVal: 2.651 ± 0.066	0.534PheTrp: 0.534 ± 0.032	1.167PheTyr: 1.167 ± 0.038	0.001PheXaa: 0.001 ± 0.001
Gly	5.284GlyAla: 5.284 ± 0.09	0.764GlyCys: 0.764 ± 0.034	3.632GlyAsp: 3.632 ± 0.074	4.234GlyGlu: 4.234 ± 0.073	3.145GlyPhe: 3.145 ± 0.071	4.632GlyGly: 4.632 ± 0.092	1.81GlyHis: 1.81 ± 0.048	4.586GlyIle: 4.586 ± 0.079	3.296GlyLys: 3.296 ± 0.071	7.634GlyLeu: 7.634 ± 0.107	2.137GlyMet: 2.137 ± 0.049	2.331GlyAsn: 2.331 ± 0.063	2.001GlyPro: 2.001 ± 0.052	3.337GlyGln: 3.337 ± 0.074	4.095GlyArg: 4.095 ± 0.077	3.917GlySer: 3.917 ± 0.07	3.294GlyThr: 3.294 ± 0.067	4.914GlyVal: 4.914 ± 0.086	1.021GlyTrp: 1.021 ± 0.039	2.324GlyTyr: 2.324 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	2.092HisAla: 2.092 ± 0.061	0.389HisCys: 0.389 ± 0.019	1.38HisAsp: 1.38 ± 0.045	1.292HisGlu: 1.292 ± 0.04	0.981HisPhe: 0.981 ± 0.03	1.818HisGly: 1.818 ± 0.054	0.868HisHis: 0.868 ± 0.04	1.471HisIle: 1.471 ± 0.048	0.899HisLys: 0.899 ± 0.032	2.434HisLeu: 2.434 ± 0.054	0.567HisMet: 0.567 ± 0.027	0.881HisAsn: 0.881 ± 0.035	1.345HisPro: 1.345 ± 0.049	1.557HisGln: 1.557 ± 0.052	1.505HisArg: 1.505 ± 0.043	1.444HisSer: 1.444 ± 0.046	1.079HisThr: 1.079 ± 0.038	1.386HisVal: 1.386 ± 0.048	0.438HisTrp: 0.438 ± 0.027	0.884HisTyr: 0.884 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.496IleAla: 6.496 ± 0.097	0.589IleCys: 0.589 ± 0.03	4.2IleAsp: 4.2 ± 0.081	4.536IleGlu: 4.536 ± 0.079	1.959IlePhe: 1.959 ± 0.044	4.389IleGly: 4.389 ± 0.084	1.478IleHis: 1.478 ± 0.043	3.559IleIle: 3.559 ± 0.077	2.455IleLys: 2.455 ± 0.057	5.173IleLeu: 5.173 ± 0.086	1.355IleMet: 1.355 ± 0.043	2.793IleAsn: 2.793 ± 0.066	2.561IlePro: 2.561 ± 0.06	2.345IleGln: 2.345 ± 0.053	3.546IleArg: 3.546 ± 0.065	4.381IleSer: 4.381 ± 0.076	3.297IleThr: 3.297 ± 0.069	3.909IleVal: 3.909 ± 0.07	0.663IleTrp: 0.663 ± 0.031	1.605IleTyr: 1.605 ± 0.052	0.005IleXaa: 0.005 ± 0.002
Lys	3.747LysAla: 3.747 ± 0.082	0.218LysCys: 0.218 ± 0.019	1.876LysAsp: 1.876 ± 0.055	1.73LysGlu: 1.73 ± 0.051	1.11LysPhe: 1.11 ± 0.038	2.301LysGly: 2.301 ± 0.061	1.125LysHis: 1.125 ± 0.038	2.296LysIle: 2.296 ± 0.059	1.815LysLys: 1.815 ± 0.064	4.303LysLeu: 4.303 ± 0.073	1.046LysMet: 1.046 ± 0.04	1.385LysAsn: 1.385 ± 0.05	1.943LysPro: 1.943 ± 0.059	2.57LysGln: 2.57 ± 0.069	2.267LysArg: 2.267 ± 0.057	2.334LysSer: 2.334 ± 0.057	2.328LysThr: 2.328 ± 0.059	2.664LysVal: 2.664 ± 0.067	0.403LysTrp: 0.403 ± 0.031	0.86LysTyr: 0.86 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	11.017LeuAla: 11.017 ± 0.16	0.977LeuCys: 0.977 ± 0.038	6.146LeuAsp: 6.146 ± 0.095	6.253LeuGlu: 6.253 ± 0.118	4.137LeuPhe: 4.137 ± 0.089	6.941LeuGly: 6.941 ± 0.1	2.49LeuHis: 2.49 ± 0.064	6.582LeuIle: 6.582 ± 0.12	4.522LeuLys: 4.522 ± 0.078	12.725LeuLeu: 12.725 ± 0.215	3.09LeuMet: 3.09 ± 0.073	4.219LeuAsn: 4.219 ± 0.083	5.461LeuPro: 5.461 ± 0.088	5.153LeuGln: 5.153 ± 0.103	5.903LeuArg: 5.903 ± 0.091	7.945LeuSer: 7.945 ± 0.112	5.974LeuThr: 5.974 ± 0.087	6.869LeuVal: 6.869 ± 0.108	1.282LeuTrp: 1.282 ± 0.047	2.369LeuTyr: 2.369 ± 0.059	0.003LeuXaa: 0.003 ± 0.002
Met	2.825MetAla: 2.825 ± 0.064	0.176MetCys: 0.176 ± 0.015	1.391MetAsp: 1.391 ± 0.048	1.263MetGlu: 1.263 ± 0.042	0.933MetPhe: 0.933 ± 0.037	1.709MetGly: 1.709 ± 0.049	0.62MetHis: 0.62 ± 0.032	1.617MetIle: 1.617 ± 0.042	1.245MetLys: 1.245 ± 0.038	3.241MetLeu: 3.241 ± 0.077	0.882MetMet: 0.882 ± 0.04	1.045MetAsn: 1.045 ± 0.036	1.411MetPro: 1.411 ± 0.041	1.563MetGln: 1.563 ± 0.045	1.552MetArg: 1.552 ± 0.044	1.85MetSer: 1.85 ± 0.049	1.871MetThr: 1.871 ± 0.043	1.726MetVal: 1.726 ± 0.052	0.209MetTrp: 0.209 ± 0.016	0.434MetTyr: 0.434 ± 0.023	0.003MetXaa: 0.003 ± 0.002
Asn	3.327AsnAla: 3.327 ± 0.076	0.295AsnCys: 0.295 ± 0.021	1.975AsnAsp: 1.975 ± 0.056	1.982AsnGlu: 1.982 ± 0.048	1.351AsnPhe: 1.351 ± 0.041	2.48AsnGly: 2.48 ± 0.063	0.953AsnHis: 0.953 ± 0.035	2.421AsnIle: 2.421 ± 0.056	1.363AsnLys: 1.363 ± 0.045	3.608AsnLeu: 3.608 ± 0.065	0.859AsnMet: 0.859 ± 0.031	1.497AsnAsn: 1.497 ± 0.046	2.093AsnPro: 2.093 ± 0.057	1.893AsnGln: 1.893 ± 0.052	2.08AsnArg: 2.08 ± 0.055	2.174AsnSer: 2.174 ± 0.065	2.008AsnThr: 2.008 ± 0.049	2.256AsnVal: 2.256 ± 0.066	0.509AsnTrp: 0.509 ± 0.026	1.015AsnTyr: 1.015 ± 0.042	0.001AsnXaa: 0.001 ± 0.001
Pro	4.157ProAla: 4.157 ± 0.071	0.358ProCys: 0.358 ± 0.022	3.106ProAsp: 3.106 ± 0.06	3.849ProGlu: 3.849 ± 0.083	1.656ProPhe: 1.656 ± 0.05	3.168ProGly: 3.168 ± 0.068	0.929ProHis: 0.929 ± 0.04	2.089ProIle: 2.089 ± 0.052	1.342ProLys: 1.342 ± 0.042	4.34ProLeu: 4.34 ± 0.086	1.055ProMet: 1.055 ± 0.034	1.257ProAsn: 1.257 ± 0.04	1.399ProPro: 1.399 ± 0.046	1.778ProGln: 1.778 ± 0.049	1.899ProArg: 1.899 ± 0.053	2.247ProSer: 2.247 ± 0.052	1.827ProThr: 1.827 ± 0.057	3.763ProVal: 3.763 ± 0.068	0.517ProTrp: 0.517 ± 0.027	1.139ProTyr: 1.139 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	5.469GlnAla: 5.469 ± 0.103	0.474GlnCys: 0.474 ± 0.025	2.103GlnAsp: 2.103 ± 0.062	2.216GlnGlu: 2.216 ± 0.062	1.792GlnPhe: 1.792 ± 0.048	2.958GlnGly: 2.958 ± 0.054	1.634GlnHis: 1.634 ± 0.049	2.997GlnIle: 2.997 ± 0.067	1.727GlnLys: 1.727 ± 0.044	6.593GlnLeu: 6.593 ± 0.12	1.364GlnMet: 1.364 ± 0.039	1.464GlnAsn: 1.464 ± 0.048	2.236GlnPro: 2.236 ± 0.056	4.838GlnGln: 4.838 ± 0.122	3.683GlnArg: 3.683 ± 0.09	3.124GlnSer: 3.124 ± 0.074	2.674GlnThr: 2.674 ± 0.06	3.173GlnVal: 3.173 ± 0.063	0.802GlnTrp: 0.802 ± 0.033	1.22GlnTyr: 1.22 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	4.409ArgAla: 4.409 ± 0.074	0.53ArgCys: 0.53 ± 0.029	3.291ArgAsp: 3.291 ± 0.063	3.604ArgGlu: 3.604 ± 0.078	2.57ArgPhe: 2.57 ± 0.054	3.28ArgGly: 3.28 ± 0.059	1.624ArgHis: 1.624 ± 0.044	3.537ArgIle: 3.537 ± 0.067	2.177ArgLys: 2.177 ± 0.055	6.855ArgLeu: 6.855 ± 0.106	1.67ArgMet: 1.67 ± 0.043	2.129ArgAsn: 2.129 ± 0.05	2.163ArgPro: 2.163 ± 0.05	3.688ArgGln: 3.688 ± 0.079	3.52ArgArg: 3.52 ± 0.083	3.147ArgSer: 3.147 ± 0.062	2.381ArgThr: 2.381 ± 0.055	3.63ArgVal: 3.63 ± 0.073	0.91ArgTrp: 0.91 ± 0.035	2.097ArgTyr: 2.097 ± 0.053	0.003ArgXaa: 0.003 ± 0.002
Ser	5.861SerAla: 5.861 ± 0.086	0.563SerCys: 0.563 ± 0.026	3.579SerAsp: 3.579 ± 0.065	3.852SerGlu: 3.852 ± 0.074	2.47SerPhe: 2.47 ± 0.062	5.088SerGly: 5.088 ± 0.102	1.592SerHis: 1.592 ± 0.044	3.486SerIle: 3.486 ± 0.065	2.222SerLys: 2.222 ± 0.064	6.936SerLeu: 6.936 ± 0.108	1.748SerMet: 1.748 ± 0.056	2.089SerAsn: 2.089 ± 0.056	2.559SerPro: 2.559 ± 0.051	2.942SerGln: 2.942 ± 0.071	3.655SerArg: 3.655 ± 0.064	3.942SerSer: 3.942 ± 0.089	2.881SerThr: 2.881 ± 0.069	4.456SerVal: 4.456 ± 0.074	0.816SerTrp: 0.816 ± 0.03	1.683SerTyr: 1.683 ± 0.05	0.001SerXaa: 0.001 ± 0.001
Thr	5.113ThrAla: 5.113 ± 0.081	0.371ThrCys: 0.371 ± 0.028	3.119ThrAsp: 3.119 ± 0.06	3.369ThrGlu: 3.369 ± 0.068	1.736ThrPhe: 1.736 ± 0.045	4.483ThrGly: 4.483 ± 0.082	1.136ThrHis: 1.136 ± 0.037	2.916ThrIle: 2.916 ± 0.069	1.222ThrLys: 1.222 ± 0.052	6.333ThrLeu: 6.333 ± 0.09	1.183ThrMet: 1.183 ± 0.041	1.508ThrAsn: 1.508 ± 0.052	2.572ThrPro: 2.572 ± 0.058	2.13ThrGln: 2.13 ± 0.053	2.526ThrArg: 2.526 ± 0.057	2.921ThrSer: 2.921 ± 0.066	2.672ThrThr: 2.672 ± 0.076	3.779ThrVal: 3.779 ± 0.073	0.46ThrTrp: 0.46 ± 0.027	1.059ThrTyr: 1.059 ± 0.035	0.005ThrXaa: 0.005 ± 0.003
Val	6.386ValAla: 6.386 ± 0.098	0.686ValCys: 0.686 ± 0.03	4.13ValAsp: 4.13 ± 0.079	4.32ValGlu: 4.32 ± 0.081	2.833ValPhe: 2.833 ± 0.063	4.289ValGly: 4.289 ± 0.079	1.338ValHis: 1.338 ± 0.043	4.816ValIle: 4.816 ± 0.09	2.511ValLys: 2.511 ± 0.062	7.064ValLeu: 7.064 ± 0.12	2.187ValMet: 2.187 ± 0.058	2.648ValAsn: 2.648 ± 0.069	2.659ValPro: 2.659 ± 0.062	2.422ValGln: 2.422 ± 0.055	3.665ValArg: 3.665 ± 0.07	4.632ValSer: 4.632 ± 0.071	3.863ValThr: 3.863 ± 0.088	5.137ValVal: 5.137 ± 0.092	0.84ValTrp: 0.84 ± 0.037	1.709ValTyr: 1.709 ± 0.045	0.005ValXaa: 0.005 ± 0.003
Trp	0.879TrpAla: 0.879 ± 0.038	0.141TrpCys: 0.141 ± 0.014	0.545TrpAsp: 0.545 ± 0.026	0.47TrpGlu: 0.47 ± 0.027	0.575TrpPhe: 0.575 ± 0.029	0.711TrpGly: 0.711 ± 0.031	0.45TrpHis: 0.45 ± 0.023	0.639TrpIle: 0.639 ± 0.031	0.399TrpLys: 0.399 ± 0.028	2.119TrpLeu: 2.119 ± 0.069	0.317TrpMet: 0.317 ± 0.018	0.368TrpAsn: 0.368 ± 0.022	0.597TrpPro: 0.597 ± 0.033	1.291TrpGln: 1.291 ± 0.054	0.804TrpArg: 0.804 ± 0.034	0.714TrpSer: 0.714 ± 0.033	0.553TrpThr: 0.553 ± 0.025	0.752TrpVal: 0.752 ± 0.032	0.192TrpTrp: 0.192 ± 0.016	0.375TrpTyr: 0.375 ± 0.035	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.294TyrAla: 2.294 ± 0.049	0.298TyrCys: 0.298 ± 0.021	1.505TyrAsp: 1.505 ± 0.045	1.344TyrGlu: 1.344 ± 0.053	1.15TyrPhe: 1.15 ± 0.039	1.991TyrGly: 1.991 ± 0.054	0.73TyrHis: 0.73 ± 0.03	1.221TyrIle: 1.221 ± 0.044	0.787TyrLys: 0.787 ± 0.035	3.169TyrLeu: 3.169 ± 0.063	0.491TyrMet: 0.491 ± 0.025	0.842TyrAsn: 0.842 ± 0.031	1.238TyrPro: 1.238 ± 0.036	2.036TyrGln: 2.036 ± 0.055	1.995TyrArg: 1.995 ± 0.052	1.555TyrSer: 1.555 ± 0.049	1.239TyrThr: 1.239 ± 0.047	1.575TyrVal: 1.575 ± 0.042	0.397TyrTrp: 0.397 ± 0.022	0.813TyrTyr: 0.813 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.003XaaIle: 0.003 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.005XaaLeu: 0.005 ± 0.003	0.003XaaMet: 0.003 ± 0.002	0.001XaaAsn: 0.001 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.004XaaThr: 0.004 ± 0.002	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.003XaaTyr: 0.003 ± 0.002	0.008XaaXaa: 0.008 ± 0.004

Statistics based on 2536 proteins (774044 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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