Gordonia phage Gmala1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordtnkvirus; unclassified Gordtnkvirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3X9Q5|A0A0E3X9Q5_9CAUD Pre-TMP frameshift protein OS=Gordonia phage Gmala1 OX=1622190 GN=Gmala1_15 PE=4 SV=1
MM1 pKa = 7.08LTLVTALMVAALNLTPHH18 pKa = 7.01GDD20 pKa = 3.45VTLPIYY26 pKa = 10.34PCGDD30 pKa = 4.61GIPVYY35 pKa = 10.06VAQDD39 pKa = 3.29GTVYY43 pKa = 11.08GDD45 pKa = 3.32QNNNGTIDD53 pKa = 3.64PAEE56 pKa = 4.43CLDD59 pKa = 3.71TT60 pKa = 4.69

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3XBE1|A0A0E3XBE1_9CAUD Uncharacterized protein OS=Gordonia phage Gmala1 OX=1622190 GN=Gmala1_4 PE=4 SV=1
MM1 pKa = 7.25SAIHH5 pKa = 6.28TGPANMLGSVLNRR18 pKa = 11.84TEE20 pKa = 4.33HH21 pKa = 6.32IGRR24 pKa = 11.84IYY26 pKa = 8.95EE27 pKa = 4.28TAVSTKK33 pKa = 9.59IYY35 pKa = 9.84RR36 pKa = 11.84ARR38 pKa = 11.84EE39 pKa = 3.38RR40 pKa = 11.84AEE42 pKa = 3.83FNKK45 pKa = 10.16SVRR48 pKa = 11.84LHH50 pKa = 5.47FQPVRR55 pKa = 11.84FHH57 pKa = 7.24SSQQ60 pKa = 2.96

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

22691

50

3272

252.1

27.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.008 ± 0.908

0.974 ± 0.18

6.624 ± 0.431

6.249 ± 0.311

3.953 ± 0.131

7.695 ± 0.487

1.719 ± 0.211

5.808 ± 0.115

5.791 ± 0.241

7.324 ± 0.206

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.144

4.998 ± 0.154

4.768 ± 0.346

3.768 ± 0.207

5.068 ± 0.247

6.725 ± 0.257

5.773 ± 0.207

6.549 ± 0.21

1.481 ± 0.113

2.931 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski