Bacillus sp. MUM 116

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2QRS6|A0A1S2QRS6_9BACI Chaperone protein ClpB OS=Bacillus sp. MUM 116 OX=1678002 GN=clpB PE=3 SV=1
MM1 pKa = 6.98NWNWNEE7 pKa = 3.72TAINLPYY14 pKa = 10.62TFDD17 pKa = 3.6NLKK20 pKa = 10.61EE21 pKa = 3.92FLNNICNKK29 pKa = 9.86KK30 pKa = 10.62NNFSQYY36 pKa = 10.91EE37 pKa = 4.02FVKK40 pKa = 10.03WCDD43 pKa = 3.63NLTMTLDD50 pKa = 4.43DD51 pKa = 6.16DD52 pKa = 6.04DD53 pKa = 5.89DD54 pKa = 5.28LSEE57 pKa = 4.08MDD59 pKa = 4.48EE60 pKa = 4.74LAFCLHH66 pKa = 7.34EE67 pKa = 4.12II68 pKa = 4.46

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2R7V6|A0A1S2R7V6_9BACI Uncharacterized protein OS=Bacillus sp. MUM 116 OX=1678002 GN=BIV60_13805 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.7VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5271

0

5271

1518309

26

2206

288.0

32.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.88 ± 0.033

0.758 ± 0.01

4.72 ± 0.028

7.024 ± 0.041

4.827 ± 0.031

7.045 ± 0.029

2.069 ± 0.015

8.209 ± 0.034

7.285 ± 0.03

9.864 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.015

4.61 ± 0.023

3.702 ± 0.022

3.586 ± 0.022

3.858 ± 0.025

6.042 ± 0.025

5.316 ± 0.028

6.902 ± 0.027

1.056 ± 0.011

3.502 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski