Corynebacterium choanis
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2308 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G6J5H9|A0A3G6J5H9_9CORY Zinc import ATP-binding protein ZnuC OS=Corynebacterium choanis OX=1862358 GN=znuC PE=4 SV=1
MM1 pKa = 7.68 SFYY4 pKa = 11.04 EE5 pKa = 4.52 DD6 pKa = 3.45 LASALDD12 pKa = 3.72 EE13 pKa = 4.05 NRR15 pKa = 11.84 IEE17 pKa = 4.06 QRR19 pKa = 11.84 IVDD22 pKa = 3.87 GAMYY26 pKa = 10.19 IPTPSGIDD34 pKa = 3.36 LKK36 pKa = 10.99 ILVDD40 pKa = 4.03 NEE42 pKa = 4.05 VLPAASIYY50 pKa = 10.86 LLDD53 pKa = 4.49 YY54 pKa = 11.13 GDD56 pKa = 3.85 STSEE60 pKa = 4.37 LEE62 pKa = 4.3 SSTLLEE68 pKa = 4.01 VAFSVAGATAAIRR81 pKa = 11.84 TFLEE85 pKa = 4.2 TSQALSFIDD94 pKa = 5.37 DD95 pKa = 4.4 LVDD98 pKa = 4.34 DD99 pKa = 4.42 PLGRR103 pKa = 11.84 FPEE106 pKa = 4.14 LAFASPEE113 pKa = 4.06 TGVPIAYY120 pKa = 9.37 APCGNQGLLVVEE132 pKa = 4.6 ITTIGSMQQAAVSLLLAPEE151 pKa = 4.58 SLLEE155 pKa = 4.08 MLSQQQTPSGATDD168 pKa = 3.49 DD169 pKa = 4.11 AVYY172 pKa = 10.7 AVFDD176 pKa = 4.12 EE177 pKa = 4.46 LTAPGSHH184 pKa = 6.67 TYY186 pKa = 9.6 SAYY189 pKa = 10.35 VSLGSFEE196 pKa = 4.95 DD197 pKa = 4.08 FGRR200 pKa = 11.84 LSTVLPFMATQARR213 pKa = 11.84 HH214 pKa = 5.49 WEE216 pKa = 3.97 QQLAVSLARR225 pKa = 11.84 GDD227 pKa = 3.53 AAAIPEE233 pKa = 4.32 DD234 pKa = 4.06 MVAALLGLAA243 pKa = 4.61
Molecular weight: 25.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.528
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.63
Rodwell 3.567
Grimsley 3.439
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.024
Thurlkill 3.579
EMBOSS 3.643
Sillero 3.846
Patrickios 1.85
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A3G6JEK9|A0A3G6JEK9_9CORY Na(+)/H(+) antiporter subunit C1 OS=Corynebacterium choanis OX=1862358 GN=mnhC1 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2308
0
2308
778825
29
3914
337.4
36.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.417 ± 0.072
1.014 ± 0.018
5.98 ± 0.047
5.1 ± 0.05
3.089 ± 0.032
7.889 ± 0.048
2.449 ± 0.035
4.97 ± 0.04
2.886 ± 0.043
9.276 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.034 ± 0.027
2.767 ± 0.038
5.742 ± 0.049
4.201 ± 0.037
5.806 ± 0.057
5.605 ± 0.035
7.107 ± 0.083
8.239 ± 0.053
1.279 ± 0.023
2.151 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here