Coprococcus sp. AF21-14LB
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2287 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A412LDS9|A0A412LDS9_9FIRM LysM peptidoglycan-binding domain-containing protein OS=Coprococcus sp. AF21-14LB OX=2292231 GN=DWX73_05705 PE=4 SV=1
MM1 pKa = 7.43 VDD3 pKa = 3.3 YY4 pKa = 10.8 FEE6 pKa = 4.7 QEE8 pKa = 4.05 GFISEE13 pKa = 4.28 DD14 pKa = 3.89 CEE16 pKa = 3.78 PVDD19 pKa = 3.85 INEE22 pKa = 4.26 TTGYY26 pKa = 8.81 LTDD29 pKa = 3.54 NTNGEE34 pKa = 4.16 FTEE37 pKa = 4.4 TKK39 pKa = 10.55 VADD42 pKa = 3.45 KK43 pKa = 10.9 AYY45 pKa = 10.23 DD46 pKa = 3.86 YY47 pKa = 11.34 EE48 pKa = 4.44 GLWLFWWDD56 pKa = 3.77 QEE58 pKa = 4.67 NKK60 pKa = 9.12 TDD62 pKa = 4.34 LYY64 pKa = 11.57 GNYY67 pKa = 9.96 EE68 pKa = 4.16 SMAANQGTIVLAGGAAVLEE87 pKa = 4.51 TEE89 pKa = 4.57 AANGAYY95 pKa = 10.01 AIAFSEE101 pKa = 4.7 DD102 pKa = 3.5 YY103 pKa = 10.38 EE104 pKa = 4.4 KK105 pKa = 11.23 KK106 pKa = 10.1 QDD108 pKa = 3.31 AVKK111 pKa = 10.78 AFEE114 pKa = 4.15 TLKK117 pKa = 11.29 NEE119 pKa = 4.08
Molecular weight: 13.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.808
IPC_protein 3.745
Toseland 3.567
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.923
Wikipedia 3.617
Rodwell 3.579
Grimsley 3.478
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 3.973
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.859
Patrickios 0.896
IPC_peptide 3.694
IPC2_peptide 3.846
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A412LBN3|A0A412LBN3_9FIRM MurR/RpiR family transcriptional regulator OS=Coprococcus sp. AF21-14LB OX=2292231 GN=DWX73_08375 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.86 KK9 pKa = 8.2 RR10 pKa = 11.84 SRR12 pKa = 11.84 AKK14 pKa = 9.27 VHH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTAGGRR28 pKa = 11.84 KK29 pKa = 8.71 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 KK40 pKa = 8.18 QLSAA44 pKa = 3.9
Molecular weight: 4.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2287
0
2287
690620
34
1509
302.0
34.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.338 ± 0.053
1.562 ± 0.023
5.114 ± 0.035
8.426 ± 0.061
4.154 ± 0.036
6.997 ± 0.044
1.838 ± 0.024
7.568 ± 0.05
7.023 ± 0.047
9.068 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.228 ± 0.027
4.002 ± 0.037
3.245 ± 0.028
3.404 ± 0.027
4.574 ± 0.044
5.42 ± 0.036
5.218 ± 0.038
6.835 ± 0.04
0.915 ± 0.017
4.072 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here