Amino acid dipepetide frequency for Gordonia phage Mulch

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.25AlaAla: 21.25 ± 1.862
0.629AlaCys: 0.629 ± 0.214
9.053AlaAsp: 9.053 ± 0.876
7.481AlaGlu: 7.481 ± 0.912
3.395AlaPhe: 3.395 ± 0.559
15.026AlaGly: 15.026 ± 1.436
2.075AlaHis: 2.075 ± 0.409
4.841AlaIle: 4.841 ± 0.384
3.395AlaLys: 3.395 ± 0.609
9.619AlaLeu: 9.619 ± 0.794
2.075AlaMet: 2.075 ± 0.481
3.772AlaAsn: 3.772 ± 0.581
7.293AlaPro: 7.293 ± 1.227
3.709AlaGln: 3.709 ± 0.587
8.927AlaArg: 8.927 ± 0.993
6.224AlaSer: 6.224 ± 0.65
7.041AlaThr: 7.041 ± 0.768
9.43AlaVal: 9.43 ± 0.835
2.2AlaTrp: 2.2 ± 0.423
2.138AlaTyr: 2.138 ± 0.312
0.0AlaXaa: 0.0 ± 0.0
Cys
1.069CysAla: 1.069 ± 0.269
0.063CysCys: 0.063 ± 0.053
0.503CysAsp: 0.503 ± 0.189
0.566CysGlu: 0.566 ± 0.223
0.063CysPhe: 0.063 ± 0.065
1.697CysGly: 1.697 ± 0.435
0.063CysHis: 0.063 ± 0.058
0.126CysIle: 0.126 ± 0.085
0.189CysLys: 0.189 ± 0.101
0.566CysLeu: 0.566 ± 0.182
0.063CysMet: 0.063 ± 0.063
0.503CysAsn: 0.503 ± 0.213
0.754CysPro: 0.754 ± 0.219
0.126CysGln: 0.126 ± 0.098
0.692CysArg: 0.692 ± 0.202
0.377CysSer: 0.377 ± 0.136
0.692CysThr: 0.692 ± 0.231
0.44CysVal: 0.44 ± 0.192
0.251CysTrp: 0.251 ± 0.131
0.126CysTyr: 0.126 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
7.419AspAla: 7.419 ± 0.559
0.629AspCys: 0.629 ± 0.262
6.287AspAsp: 6.287 ± 0.904
3.709AspGlu: 3.709 ± 0.523
1.383AspPhe: 1.383 ± 0.33
7.733AspGly: 7.733 ± 0.829
1.509AspHis: 1.509 ± 0.273
2.892AspIle: 2.892 ± 0.483
2.138AspLys: 2.138 ± 0.381
5.847AspLeu: 5.847 ± 0.531
1.383AspMet: 1.383 ± 0.285
2.263AspAsn: 2.263 ± 0.369
4.087AspPro: 4.087 ± 0.529
3.269AspGln: 3.269 ± 0.359
4.841AspArg: 4.841 ± 0.765
3.395AspSer: 3.395 ± 0.405
4.778AspThr: 4.778 ± 0.626
5.281AspVal: 5.281 ± 0.589
1.697AspTrp: 1.697 ± 0.287
0.943AspTyr: 0.943 ± 0.236
0.0AspXaa: 0.0 ± 0.0
Glu
4.841GluAla: 4.841 ± 0.581
0.44GluCys: 0.44 ± 0.141
2.829GluAsp: 2.829 ± 0.531
2.012GluGlu: 2.012 ± 0.472
1.132GluPhe: 1.132 ± 0.238
2.452GluGly: 2.452 ± 0.375
1.32GluHis: 1.32 ± 0.304
2.263GluIle: 2.263 ± 0.3
2.012GluLys: 2.012 ± 0.402
6.224GluLeu: 6.224 ± 0.58
1.132GluMet: 1.132 ± 0.288
2.012GluAsn: 2.012 ± 0.336
3.332GluPro: 3.332 ± 0.661
2.578GluGln: 2.578 ± 0.429
4.778GluArg: 4.778 ± 0.676
3.332GluSer: 3.332 ± 0.511
3.646GluThr: 3.646 ± 0.507
3.584GluVal: 3.584 ± 0.657
1.32GluTrp: 1.32 ± 0.27
1.32GluTyr: 1.32 ± 0.345
0.0GluXaa: 0.0 ± 0.0
Phe
4.275PheAla: 4.275 ± 0.438
0.126PheCys: 0.126 ± 0.074
2.138PheAsp: 2.138 ± 0.279
1.949PheGlu: 1.949 ± 0.305
0.377PhePhe: 0.377 ± 0.164
2.075PheGly: 2.075 ± 0.403
0.189PheHis: 0.189 ± 0.11
0.943PheIle: 0.943 ± 0.266
0.817PheLys: 0.817 ± 0.269
1.257PheLeu: 1.257 ± 0.294
0.44PheMet: 0.44 ± 0.139
0.44PheAsn: 0.44 ± 0.206
1.069PhePro: 1.069 ± 0.28
0.503PheGln: 0.503 ± 0.213
1.886PheArg: 1.886 ± 0.297
2.012PheSer: 2.012 ± 0.329
1.949PheThr: 1.949 ± 0.359
2.326PheVal: 2.326 ± 0.491
0.377PheTrp: 0.377 ± 0.152
0.44PheTyr: 0.44 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
10.499GlyAla: 10.499 ± 1.043
0.943GlyCys: 0.943 ± 0.264
7.481GlyAsp: 7.481 ± 0.892
3.521GlyGlu: 3.521 ± 0.487
2.892GlyPhe: 2.892 ± 0.45
9.556GlyGly: 9.556 ± 1.497
2.892GlyHis: 2.892 ± 0.484
5.092GlyIle: 5.092 ± 0.902
2.955GlyLys: 2.955 ± 0.394
7.167GlyLeu: 7.167 ± 1.043
1.132GlyMet: 1.132 ± 0.285
2.075GlyAsn: 2.075 ± 0.406
4.212GlyPro: 4.212 ± 0.537
2.892GlyGln: 2.892 ± 0.421
7.104GlyArg: 7.104 ± 0.641
5.784GlySer: 5.784 ± 0.575
6.978GlyThr: 6.978 ± 0.767
6.224GlyVal: 6.224 ± 0.841
1.446GlyTrp: 1.446 ± 0.288
2.389GlyTyr: 2.389 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
2.892HisAla: 2.892 ± 0.5
0.189HisCys: 0.189 ± 0.111
1.76HisAsp: 1.76 ± 0.363
1.006HisGlu: 1.006 ± 0.263
0.503HisPhe: 0.503 ± 0.215
1.572HisGly: 1.572 ± 0.273
0.754HisHis: 0.754 ± 0.285
0.943HisIle: 0.943 ± 0.289
0.692HisLys: 0.692 ± 0.174
1.635HisLeu: 1.635 ± 0.321
0.189HisMet: 0.189 ± 0.131
0.503HisAsn: 0.503 ± 0.168
1.509HisPro: 1.509 ± 0.319
0.251HisGln: 0.251 ± 0.113
1.446HisArg: 1.446 ± 0.417
0.503HisSer: 0.503 ± 0.166
1.446HisThr: 1.446 ± 0.271
2.075HisVal: 2.075 ± 0.346
0.189HisTrp: 0.189 ± 0.098
0.251HisTyr: 0.251 ± 0.11
0.0HisXaa: 0.0 ± 0.0
Ile
6.476IleAla: 6.476 ± 0.558
0.251IleCys: 0.251 ± 0.124
3.584IleAsp: 3.584 ± 0.43
3.269IleGlu: 3.269 ± 0.373
0.566IlePhe: 0.566 ± 0.21
4.275IleGly: 4.275 ± 0.747
0.566IleHis: 0.566 ± 0.197
0.503IleIle: 0.503 ± 0.196
1.509IleLys: 1.509 ± 0.492
2.326IleLeu: 2.326 ± 0.336
0.503IleMet: 0.503 ± 0.15
0.754IleAsn: 0.754 ± 0.207
2.012IlePro: 2.012 ± 0.367
0.88IleGln: 0.88 ± 0.257
3.332IleArg: 3.332 ± 0.457
1.383IleSer: 1.383 ± 0.329
3.143IleThr: 3.143 ± 0.355
3.332IleVal: 3.332 ± 0.536
1.132IleTrp: 1.132 ± 0.268
0.377IleTyr: 0.377 ± 0.138
0.0IleXaa: 0.0 ± 0.0
Lys
3.584LysAla: 3.584 ± 0.62
0.314LysCys: 0.314 ± 0.14
1.069LysAsp: 1.069 ± 0.283
0.943LysGlu: 0.943 ± 0.22
0.88LysPhe: 0.88 ± 0.222
2.515LysGly: 2.515 ± 0.441
0.692LysHis: 0.692 ± 0.179
1.635LysIle: 1.635 ± 0.306
0.503LysLys: 0.503 ± 0.219
2.641LysLeu: 2.641 ± 0.541
0.817LysMet: 0.817 ± 0.191
0.88LysAsn: 0.88 ± 0.253
2.075LysPro: 2.075 ± 0.384
0.44LysGln: 0.44 ± 0.153
2.012LysArg: 2.012 ± 0.432
1.76LysSer: 1.76 ± 0.307
1.635LysThr: 1.635 ± 0.366
2.452LysVal: 2.452 ± 0.328
0.44LysTrp: 0.44 ± 0.161
0.566LysTyr: 0.566 ± 0.194
0.0LysXaa: 0.0 ± 0.0
Leu
9.682LeuAla: 9.682 ± 0.74
0.503LeuCys: 0.503 ± 0.175
5.595LeuAsp: 5.595 ± 0.549
5.973LeuGlu: 5.973 ± 0.617
2.263LeuPhe: 2.263 ± 0.322
6.79LeuGly: 6.79 ± 0.616
1.257LeuHis: 1.257 ± 0.259
3.143LeuIle: 3.143 ± 0.387
1.32LeuLys: 1.32 ± 0.302
6.413LeuLeu: 6.413 ± 0.634
1.635LeuMet: 1.635 ± 0.349
2.515LeuAsn: 2.515 ± 0.476
4.652LeuPro: 4.652 ± 0.598
2.515LeuGln: 2.515 ± 0.41
6.601LeuArg: 6.601 ± 0.776
4.464LeuSer: 4.464 ± 0.436
5.658LeuThr: 5.658 ± 0.613
5.784LeuVal: 5.784 ± 0.552
1.446LeuTrp: 1.446 ± 0.227
1.697LeuTyr: 1.697 ± 0.339
0.0LeuXaa: 0.0 ± 0.0
Met
2.703MetAla: 2.703 ± 0.347
0.126MetCys: 0.126 ± 0.099
0.251MetAsp: 0.251 ± 0.151
0.566MetGlu: 0.566 ± 0.19
0.943MetPhe: 0.943 ± 0.269
1.006MetGly: 1.006 ± 0.282
0.189MetHis: 0.189 ± 0.094
0.817MetIle: 0.817 ± 0.191
0.566MetLys: 0.566 ± 0.213
0.754MetLeu: 0.754 ± 0.194
0.377MetMet: 0.377 ± 0.151
0.44MetAsn: 0.44 ± 0.196
1.446MetPro: 1.446 ± 0.252
0.566MetGln: 0.566 ± 0.15
0.754MetArg: 0.754 ± 0.227
1.697MetSer: 1.697 ± 0.282
2.2MetThr: 2.2 ± 0.342
1.383MetVal: 1.383 ± 0.238
0.377MetTrp: 0.377 ± 0.106
0.189MetTyr: 0.189 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
3.081AsnAla: 3.081 ± 0.573
0.189AsnCys: 0.189 ± 0.1
1.76AsnAsp: 1.76 ± 0.367
1.069AsnGlu: 1.069 ± 0.26
0.88AsnPhe: 0.88 ± 0.203
3.332AsnGly: 3.332 ± 0.548
0.629AsnHis: 0.629 ± 0.243
0.88AsnIle: 0.88 ± 0.299
1.132AsnLys: 1.132 ± 0.217
2.452AsnLeu: 2.452 ± 0.532
0.566AsnMet: 0.566 ± 0.176
0.629AsnAsn: 0.629 ± 0.196
1.886AsnPro: 1.886 ± 0.3
1.069AsnGln: 1.069 ± 0.248
2.075AsnArg: 2.075 ± 0.377
0.754AsnSer: 0.754 ± 0.198
1.446AsnThr: 1.446 ± 0.25
1.949AsnVal: 1.949 ± 0.435
0.754AsnTrp: 0.754 ± 0.166
0.629AsnTyr: 0.629 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
8.927ProAla: 8.927 ± 1.27
0.692ProCys: 0.692 ± 0.25
4.589ProAsp: 4.589 ± 0.817
2.892ProGlu: 2.892 ± 0.617
0.943ProPhe: 0.943 ± 0.203
5.533ProGly: 5.533 ± 0.667
1.572ProHis: 1.572 ± 0.336
2.075ProIle: 2.075 ± 0.385
1.949ProLys: 1.949 ± 0.329
3.143ProLeu: 3.143 ± 0.458
1.006ProMet: 1.006 ± 0.236
1.886ProAsn: 1.886 ± 0.268
3.143ProPro: 3.143 ± 0.54
2.075ProGln: 2.075 ± 0.388
3.584ProArg: 3.584 ± 0.516
2.515ProSer: 2.515 ± 0.362
4.841ProThr: 4.841 ± 0.591
4.715ProVal: 4.715 ± 0.475
1.069ProTrp: 1.069 ± 0.2
1.257ProTyr: 1.257 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
4.652GlnAla: 4.652 ± 0.784
0.189GlnCys: 0.189 ± 0.131
0.817GlnAsp: 0.817 ± 0.24
1.069GlnGlu: 1.069 ± 0.257
1.195GlnPhe: 1.195 ± 0.362
2.2GlnGly: 2.2 ± 0.426
0.754GlnHis: 0.754 ± 0.228
1.572GlnIle: 1.572 ± 0.272
0.817GlnLys: 0.817 ± 0.199
3.081GlnLeu: 3.081 ± 0.551
0.503GlnMet: 0.503 ± 0.149
0.88GlnAsn: 0.88 ± 0.247
2.2GlnPro: 2.2 ± 0.296
1.132GlnGln: 1.132 ± 0.307
2.138GlnArg: 2.138 ± 0.432
1.32GlnSer: 1.32 ± 0.38
1.509GlnThr: 1.509 ± 0.323
2.892GlnVal: 2.892 ± 0.454
0.88GlnTrp: 0.88 ± 0.324
0.44GlnTyr: 0.44 ± 0.158
0.0GlnXaa: 0.0 ± 0.0
Arg
9.493ArgAla: 9.493 ± 0.867
1.132ArgCys: 1.132 ± 0.325
5.784ArgAsp: 5.784 ± 0.638
4.527ArgGlu: 4.527 ± 0.465
2.2ArgPhe: 2.2 ± 0.506
5.721ArgGly: 5.721 ± 0.611
1.572ArgHis: 1.572 ± 0.411
4.024ArgIle: 4.024 ± 0.465
1.697ArgLys: 1.697 ± 0.323
6.224ArgLeu: 6.224 ± 0.595
1.383ArgMet: 1.383 ± 0.284
2.452ArgAsn: 2.452 ± 0.388
4.212ArgPro: 4.212 ± 0.657
1.76ArgGln: 1.76 ± 0.366
6.916ArgArg: 6.916 ± 0.879
2.892ArgSer: 2.892 ± 0.412
4.024ArgThr: 4.024 ± 0.519
5.721ArgVal: 5.721 ± 0.662
2.012ArgTrp: 2.012 ± 0.459
1.635ArgTyr: 1.635 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
6.161SerAla: 6.161 ± 0.685
0.44SerCys: 0.44 ± 0.162
2.829SerAsp: 2.829 ± 0.47
2.2SerGlu: 2.2 ± 0.451
1.635SerPhe: 1.635 ± 0.349
6.098SerGly: 6.098 ± 0.626
1.257SerHis: 1.257 ± 0.268
1.886SerIle: 1.886 ± 0.308
1.823SerLys: 1.823 ± 0.317
4.527SerLeu: 4.527 ± 0.426
0.817SerMet: 0.817 ± 0.165
1.006SerAsn: 1.006 ± 0.228
2.829SerPro: 2.829 ± 0.525
1.697SerGln: 1.697 ± 0.399
3.206SerArg: 3.206 ± 0.383
4.338SerSer: 4.338 ± 0.978
4.715SerThr: 4.715 ± 0.538
3.898SerVal: 3.898 ± 0.534
0.943SerTrp: 0.943 ± 0.205
0.88SerTyr: 0.88 ± 0.227
0.0SerXaa: 0.0 ± 0.0
Thr
8.11ThrAla: 8.11 ± 0.686
1.006ThrCys: 1.006 ± 0.266
5.47ThrAsp: 5.47 ± 0.611
3.269ThrGlu: 3.269 ± 0.661
1.76ThrPhe: 1.76 ± 0.35
6.853ThrGly: 6.853 ± 0.801
1.32ThrHis: 1.32 ± 0.279
2.641ThrIle: 2.641 ± 0.412
1.886ThrLys: 1.886 ± 0.355
5.03ThrLeu: 5.03 ± 0.612
0.943ThrMet: 0.943 ± 0.213
1.383ThrAsn: 1.383 ± 0.258
4.212ThrPro: 4.212 ± 0.657
1.509ThrGln: 1.509 ± 0.329
5.47ThrArg: 5.47 ± 0.491
3.584ThrSer: 3.584 ± 0.579
5.91ThrThr: 5.91 ± 0.799
7.104ThrVal: 7.104 ± 0.549
0.88ThrTrp: 0.88 ± 0.286
1.446ThrTyr: 1.446 ± 0.199
0.0ThrXaa: 0.0 ± 0.0
Val
10.185ValAla: 10.185 ± 0.802
0.503ValCys: 0.503 ± 0.193
6.79ValAsp: 6.79 ± 0.639
5.47ValGlu: 5.47 ± 0.585
1.823ValPhe: 1.823 ± 0.32
6.098ValGly: 6.098 ± 0.769
1.006ValHis: 1.006 ± 0.264
2.452ValIle: 2.452 ± 0.426
1.509ValLys: 1.509 ± 0.328
7.041ValLeu: 7.041 ± 0.682
1.195ValMet: 1.195 ± 0.326
1.949ValAsn: 1.949 ± 0.299
5.281ValPro: 5.281 ± 0.54
2.012ValGln: 2.012 ± 0.366
5.847ValArg: 5.847 ± 0.773
4.087ValSer: 4.087 ± 0.585
5.721ValThr: 5.721 ± 0.528
7.796ValVal: 7.796 ± 0.774
1.697ValTrp: 1.697 ± 0.362
1.257ValTyr: 1.257 ± 0.268
0.0ValXaa: 0.0 ± 0.0
Trp
1.949TrpAla: 1.949 ± 0.361
0.377TrpCys: 0.377 ± 0.163
1.32TrpAsp: 1.32 ± 0.407
0.566TrpGlu: 0.566 ± 0.183
0.44TrpPhe: 0.44 ± 0.174
1.006TrpGly: 1.006 ± 0.236
0.44TrpHis: 0.44 ± 0.172
0.817TrpIle: 0.817 ± 0.231
0.44TrpLys: 0.44 ± 0.162
2.641TrpLeu: 2.641 ± 0.391
0.566TrpMet: 0.566 ± 0.155
0.566TrpAsn: 0.566 ± 0.205
1.383TrpPro: 1.383 ± 0.27
0.503TrpGln: 0.503 ± 0.174
1.383TrpArg: 1.383 ± 0.296
1.949TrpSer: 1.949 ± 0.399
0.88TrpThr: 0.88 ± 0.246
1.446TrpVal: 1.446 ± 0.355
0.44TrpTrp: 0.44 ± 0.161
0.817TrpTyr: 0.817 ± 0.25
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.326TyrAla: 2.326 ± 0.391
0.189TyrCys: 0.189 ± 0.095
1.572TyrAsp: 1.572 ± 0.34
0.503TyrGlu: 0.503 ± 0.157
0.314TyrPhe: 0.314 ± 0.118
1.697TyrGly: 1.697 ± 0.359
0.314TyrHis: 0.314 ± 0.148
0.629TyrIle: 0.629 ± 0.256
0.503TyrLys: 0.503 ± 0.182
1.32TyrLeu: 1.32 ± 0.281
0.566TyrMet: 0.566 ± 0.169
0.251TyrAsn: 0.251 ± 0.132
0.817TyrPro: 0.817 ± 0.284
0.754TyrGln: 0.754 ± 0.222
2.389TyrArg: 2.389 ± 0.551
0.817TyrSer: 0.817 ± 0.206
1.572TyrThr: 1.572 ± 0.357
1.886TyrVal: 1.886 ± 0.4
0.377TyrTrp: 0.377 ± 0.141
0.44TyrTyr: 0.44 ± 0.191
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (15907 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski