Hainan black-spectacled toad dimarhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1GNA1|A0A2P1GNA1_9RHAB Nucleocapsid protein OS=Hainan black-spectacled toad dimarhabdovirus OX=2116358 PE=4 SV=1
MM1 pKa = 7.41SCLNKK6 pKa = 10.29RR7 pKa = 11.84FMKK10 pKa = 9.03KK11 pKa = 7.38TNRR14 pKa = 11.84DD15 pKa = 3.12NKK17 pKa = 10.01MEE19 pKa = 4.42LSNFQNLFPKK29 pKa = 10.26KK30 pKa = 9.88LLSAAVAMEE39 pKa = 4.28EE40 pKa = 4.08KK41 pKa = 9.92HH42 pKa = 6.68HH43 pKa = 6.6KK44 pKa = 10.33SSLPYY49 pKa = 10.11EE50 pKa = 4.44VINPEE55 pKa = 3.61EE56 pKa = 4.14RR57 pKa = 11.84EE58 pKa = 3.89PAKK61 pKa = 11.08VEE63 pKa = 4.08VEE65 pKa = 4.19VPEE68 pKa = 4.28TNKK71 pKa = 10.66DD72 pKa = 3.8FVPQPPEE79 pKa = 3.61KK80 pKa = 10.75ARR82 pKa = 11.84VEE84 pKa = 4.13QYY86 pKa = 11.26LGISLPKK93 pKa = 8.37TQRR96 pKa = 11.84DD97 pKa = 3.24LDD99 pKa = 4.09EE100 pKa = 4.48KK101 pKa = 11.09VKK103 pKa = 10.71EE104 pKa = 4.03LVLSILEE111 pKa = 4.47TYY113 pKa = 9.6GVNHH117 pKa = 7.08AWIKK121 pKa = 10.3VDD123 pKa = 3.8KK124 pKa = 10.82DD125 pKa = 3.15WITFQEE131 pKa = 3.75IDD133 pKa = 3.5EE134 pKa = 5.06DD135 pKa = 4.84GEE137 pKa = 4.07ALEE140 pKa = 5.1ISSSDD145 pKa = 3.52DD146 pKa = 3.43EE147 pKa = 4.68YY148 pKa = 11.6EE149 pKa = 3.84EE150 pKa = 6.5DD151 pKa = 5.55EE152 pKa = 5.23DD153 pKa = 6.26DD154 pKa = 3.83EE155 pKa = 4.82TNEE158 pKa = 4.04SDD160 pKa = 5.12EE161 pKa = 4.76SGSDD165 pKa = 3.32QKK167 pKa = 11.75GEE169 pKa = 4.05AFLQVLRR176 pKa = 11.84PEE178 pKa = 4.09QDD180 pKa = 2.77QGKK183 pKa = 10.1LDD185 pKa = 4.12FKK187 pKa = 10.98NSPSNPFASEE197 pKa = 3.87LNKK200 pKa = 9.92IEE202 pKa = 4.69KK203 pKa = 10.1DD204 pKa = 3.39LPTVLTRR211 pKa = 11.84SQVFFTQLKK220 pKa = 9.98NGFEE224 pKa = 4.36LVSISGSRR232 pKa = 11.84LWVDD236 pKa = 3.84FEE238 pKa = 4.07KK239 pKa = 11.12LKK241 pKa = 10.79IGMHH245 pKa = 6.96EE246 pKa = 3.83ILDD249 pKa = 3.75FPFRR253 pKa = 11.84EE254 pKa = 4.41DD255 pKa = 3.0WSKK258 pKa = 9.88TKK260 pKa = 10.7CLFEE264 pKa = 3.99YY265 pKa = 10.41CKK267 pKa = 10.65KK268 pKa = 10.75NKK270 pKa = 8.35TSRR273 pKa = 11.84AKK275 pKa = 10.7LGMANWGEE283 pKa = 4.33SLRR286 pKa = 11.84VDD288 pKa = 3.69PP289 pKa = 6.04

Molecular weight: 33.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1GMU0|A0A2P1GMU0_9RHAB Glycoprotein OS=Hainan black-spectacled toad dimarhabdovirus OX=2116358 PE=4 SV=1
MM1 pKa = 7.5NPLKK5 pKa = 10.54LFKK8 pKa = 10.35SGKK11 pKa = 9.42SEE13 pKa = 3.67VSPFINEE20 pKa = 3.85RR21 pKa = 11.84QPAEE25 pKa = 4.07NPYY28 pKa = 10.74GPTAPVEE35 pKa = 3.97TSSIYY40 pKa = 10.45KK41 pKa = 10.44LEE43 pKa = 4.07GKK45 pKa = 9.53ICLSLNKK52 pKa = 9.73PLEE55 pKa = 4.43DD56 pKa = 4.03PYY58 pKa = 11.85ALRR61 pKa = 11.84EE62 pKa = 3.97VLGLLEE68 pKa = 4.41KK69 pKa = 10.42NYY71 pKa = 11.11SGIHH75 pKa = 4.24QNKK78 pKa = 9.56YY79 pKa = 10.88LLLLAWNLTGCTLTRR94 pKa = 11.84GKK96 pKa = 9.95EE97 pKa = 3.97VAGVWTYY104 pKa = 7.52QTAIEE109 pKa = 4.33GFIDD113 pKa = 3.18IDD115 pKa = 4.08FNGEE119 pKa = 3.96TEE121 pKa = 4.3PCSEE125 pKa = 4.94ADD127 pKa = 3.87RR128 pKa = 11.84LWFEE132 pKa = 4.92SNVTGWTSGRR142 pKa = 11.84ICGEE146 pKa = 3.49AGFRR150 pKa = 11.84KK151 pKa = 9.89CAGCRR156 pKa = 11.84GEE158 pKa = 5.13SVTKK162 pKa = 10.72VLEE165 pKa = 3.97KK166 pKa = 10.22TGNKK170 pKa = 9.01LVPISQLLVDD180 pKa = 3.98SCIKK184 pKa = 10.55CRR186 pKa = 11.84VGTKK190 pKa = 10.3RR191 pKa = 11.84LVLYY195 pKa = 9.77KK196 pKa = 10.82AKK198 pKa = 10.73

Molecular weight: 21.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3510

198

2093

702.0

79.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.903 ± 0.884

2.194 ± 0.207

4.957 ± 0.433

7.236 ± 0.934

4.672 ± 0.366

6.866 ± 0.593

2.023 ± 0.284

7.009 ± 0.903

7.379 ± 0.552

9.459 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.263

4.986 ± 0.126

4.131 ± 0.575

2.764 ± 0.197

4.815 ± 0.345

8.006 ± 0.449

5.897 ± 0.337

5.869 ± 0.388

1.88 ± 0.112

3.647 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski