Phytoplasma mali (strain AT)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrX (Apple proliferation group); Candidatus Phytoplasma mali

Average proteome isoelectric point is 8.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3QZI4|B3QZI4_PHYMT Uncharacterized protein OS=Phytoplasma mali (strain AT) OX=482235 GN=ATP_00404 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.4KK3 pKa = 9.07TILKK7 pKa = 9.78DD8 pKa = 3.27HH9 pKa = 6.74SEE11 pKa = 4.12GLVSFTHH18 pKa = 6.31YY19 pKa = 10.67LDD21 pKa = 4.67EE22 pKa = 5.59INLFEE27 pKa = 5.41ANLEE31 pKa = 4.29GEE33 pKa = 4.79FPDD36 pKa = 3.71SALGIIANLEE46 pKa = 4.44AITYY50 pKa = 10.39LLDD53 pKa = 3.87DD54 pKa = 4.69FKK56 pKa = 11.32INTGGFDD63 pKa = 4.14FVPIDD68 pKa = 3.56YY69 pKa = 8.14STTTEE74 pKa = 3.75FDD76 pKa = 3.75DD77 pKa = 4.79VEE79 pKa = 5.48KK80 pKa = 10.35ITDD83 pKa = 3.6KK84 pKa = 11.38LNAKK88 pKa = 9.83LRR90 pKa = 11.84VAINQIKK97 pKa = 10.63DD98 pKa = 3.78GIQTLRR104 pKa = 11.84EE105 pKa = 4.16IGSHH109 pKa = 7.19LDD111 pKa = 3.65DD112 pKa = 5.48LNEE115 pKa = 4.12KK116 pKa = 10.87YY117 pKa = 10.68EE118 pKa = 4.68IIDD121 pKa = 4.21PDD123 pKa = 3.95DD124 pKa = 3.85EE125 pKa = 4.34EE126 pKa = 4.3

Molecular weight: 14.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B3QZG8|RL10_PHYMT 50S ribosomal protein L10 OS=Phytoplasma mali (strain AT) OX=482235 GN=rplJ PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.77IKK11 pKa = 10.29RR12 pKa = 11.84QRR14 pKa = 11.84THH16 pKa = 5.82GFRR19 pKa = 11.84ARR21 pKa = 11.84ISTIGGKK28 pKa = 9.6RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.86GRR39 pKa = 11.84VRR41 pKa = 11.84LTVV44 pKa = 2.92

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

448

0

448

143574

30

1522

320.5

37.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.344 ± 0.1

0.843 ± 0.036

4.722 ± 0.093

6.217 ± 0.121

6.528 ± 0.14

4.17 ± 0.106

1.607 ± 0.051

11.626 ± 0.135

11.921 ± 0.149

10.638 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.736 ± 0.043

8.284 ± 0.142

2.601 ± 0.053

3.902 ± 0.084

2.5 ± 0.078

5.84 ± 0.076

4.506 ± 0.07

4.374 ± 0.081

0.49 ± 0.03

4.153 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski