Burkholderia sp. lig30

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4988 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A063BDY5|A0A063BDY5_9BURK Uncharacterized protein OS=Burkholderia sp. lig30 OX=1192124 GN=LIG30_3782 PE=4 SV=1
MM1 pKa = 7.81EE2 pKa = 5.72ILSPMPAEE10 pKa = 4.49PSEE13 pKa = 4.01PMPAFLDD20 pKa = 3.88FTTRR24 pKa = 11.84AAAKK28 pKa = 9.35VASLIEE34 pKa = 4.29AEE36 pKa = 4.82GNPQLKK42 pKa = 10.12LRR44 pKa = 11.84LYY46 pKa = 10.6VSGGGCSGFQYY57 pKa = 10.88GFAFDD62 pKa = 4.54DD63 pKa = 4.14QPAEE67 pKa = 4.53DD68 pKa = 4.99DD69 pKa = 4.07LQIVTDD75 pKa = 5.12GVALLIDD82 pKa = 4.24AMSQQYY88 pKa = 10.82LGGARR93 pKa = 11.84VDD95 pKa = 3.78YY96 pKa = 11.25EE97 pKa = 4.16EE98 pKa = 4.72GLEE101 pKa = 4.02GSRR104 pKa = 11.84FVIQNPNAQSTCGCGSSFSVV124 pKa = 3.54

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A063BHU9|A0A063BHU9_9BURK Transcriptional regulator MerR family OS=Burkholderia sp. lig30 OX=1192124 GN=LIG30_0611 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.27RR3 pKa = 11.84SAPPRR8 pKa = 11.84MPPRR12 pKa = 11.84AIAISRR18 pKa = 11.84AAPVAWPPRR27 pKa = 11.84AATATAAALAGALWAARR44 pKa = 11.84PSAVAGAALASLAAAAGTAWLLAGWLHH71 pKa = 6.37RR72 pKa = 11.84RR73 pKa = 11.84AGRR76 pKa = 3.41

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4988

0

4988

1578848

30

4267

316.5

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.502 ± 0.058

0.952 ± 0.012

5.698 ± 0.023

4.89 ± 0.034

3.634 ± 0.021

8.328 ± 0.04

2.368 ± 0.017

4.566 ± 0.024

2.932 ± 0.026

10.152 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.018

2.647 ± 0.024

5.233 ± 0.031

3.458 ± 0.021

7.208 ± 0.04

5.315 ± 0.027

5.272 ± 0.026

7.715 ± 0.026

1.364 ± 0.015

2.448 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski