Amino acid dipepetide frequency for Gordonia phage GordTnk2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.853AlaAla: 11.853 ± 2.652
0.647AlaCys: 0.647 ± 0.221
5.647AlaAsp: 5.647 ± 0.531
6.121AlaGlu: 6.121 ± 0.567
3.06AlaPhe: 3.06 ± 0.411
7.888AlaGly: 7.888 ± 1.622
1.121AlaHis: 1.121 ± 0.222
4.698AlaIle: 4.698 ± 0.808
4.224AlaLys: 4.224 ± 0.631
6.983AlaLeu: 6.983 ± 0.722
1.897AlaMet: 1.897 ± 0.419
3.621AlaAsn: 3.621 ± 0.496
4.267AlaPro: 4.267 ± 0.554
4.095AlaGln: 4.095 ± 1.025
4.526AlaArg: 4.526 ± 0.372
5.733AlaSer: 5.733 ± 0.732
4.655AlaThr: 4.655 ± 0.581
6.207AlaVal: 6.207 ± 0.48
1.078AlaTrp: 1.078 ± 0.214
2.112AlaTyr: 2.112 ± 0.235
0.0AlaXaa: 0.0 ± 0.0
Cys
0.56CysAla: 0.56 ± 0.179
0.172CysCys: 0.172 ± 0.106
0.733CysAsp: 0.733 ± 0.233
0.819CysGlu: 0.819 ± 0.22
0.388CysPhe: 0.388 ± 0.142
0.819CysGly: 0.819 ± 0.229
0.086CysHis: 0.086 ± 0.072
0.517CysIle: 0.517 ± 0.172
0.56CysLys: 0.56 ± 0.18
0.56CysLeu: 0.56 ± 0.197
0.172CysMet: 0.172 ± 0.098
0.474CysAsn: 0.474 ± 0.182
0.647CysPro: 0.647 ± 0.223
0.431CysGln: 0.431 ± 0.157
0.733CysArg: 0.733 ± 0.286
0.69CysSer: 0.69 ± 0.203
0.474CysThr: 0.474 ± 0.181
0.603CysVal: 0.603 ± 0.154
0.172CysTrp: 0.172 ± 0.081
0.172CysTyr: 0.172 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
4.31AspAla: 4.31 ± 0.331
0.388AspCys: 0.388 ± 0.132
4.009AspAsp: 4.009 ± 0.556
4.612AspGlu: 4.612 ± 0.499
2.586AspPhe: 2.586 ± 0.376
4.353AspGly: 4.353 ± 0.494
1.81AspHis: 1.81 ± 0.415
4.138AspIle: 4.138 ± 0.328
3.707AspLys: 3.707 ± 0.402
5.0AspLeu: 5.0 ± 0.392
1.552AspMet: 1.552 ± 0.256
2.543AspAsn: 2.543 ± 0.491
4.655AspPro: 4.655 ± 0.49
2.112AspGln: 2.112 ± 0.423
2.974AspArg: 2.974 ± 0.508
4.957AspSer: 4.957 ± 1.297
3.534AspThr: 3.534 ± 0.331
3.879AspVal: 3.879 ± 0.437
1.466AspTrp: 1.466 ± 0.294
2.414AspTyr: 2.414 ± 0.459
0.0AspXaa: 0.0 ± 0.0
Glu
4.741GluAla: 4.741 ± 0.557
0.69GluCys: 0.69 ± 0.221
3.578GluAsp: 3.578 ± 0.435
4.052GluGlu: 4.052 ± 0.498
2.888GluPhe: 2.888 ± 0.477
3.147GluGly: 3.147 ± 0.384
0.991GluHis: 0.991 ± 0.243
5.129GluIle: 5.129 ± 0.538
4.44GluLys: 4.44 ± 0.458
5.517GluLeu: 5.517 ± 0.561
2.198GluMet: 2.198 ± 0.277
5.043GluAsn: 5.043 ± 0.567
2.328GluPro: 2.328 ± 0.44
2.672GluGln: 2.672 ± 0.468
2.672GluArg: 2.672 ± 0.434
4.052GluSer: 4.052 ± 0.46
3.362GluThr: 3.362 ± 0.42
3.707GluVal: 3.707 ± 0.413
1.466GluTrp: 1.466 ± 0.218
1.724GluTyr: 1.724 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
2.931PheAla: 2.931 ± 0.402
0.431PheCys: 0.431 ± 0.154
2.974PheAsp: 2.974 ± 0.335
2.198PheGlu: 2.198 ± 0.305
1.983PhePhe: 1.983 ± 0.285
3.147PheGly: 3.147 ± 0.352
0.819PheHis: 0.819 ± 0.179
1.81PheIle: 1.81 ± 0.267
2.155PheLys: 2.155 ± 0.315
2.586PheLeu: 2.586 ± 0.337
1.466PheMet: 1.466 ± 0.278
2.457PheAsn: 2.457 ± 0.248
1.552PhePro: 1.552 ± 0.317
1.207PheGln: 1.207 ± 0.243
1.724PheArg: 1.724 ± 0.238
3.448PheSer: 3.448 ± 0.478
2.457PheThr: 2.457 ± 0.32
2.543PheVal: 2.543 ± 0.292
0.603PheTrp: 0.603 ± 0.152
0.862PheTyr: 0.862 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
5.905GlyAla: 5.905 ± 1.004
0.603GlyCys: 0.603 ± 0.235
4.698GlyAsp: 4.698 ± 0.482
4.526GlyGlu: 4.526 ± 0.401
2.759GlyPhe: 2.759 ± 0.415
5.733GlyGly: 5.733 ± 1.343
1.336GlyHis: 1.336 ± 0.279
5.129GlyIle: 5.129 ± 0.395
5.302GlyLys: 5.302 ± 0.42
5.345GlyLeu: 5.345 ± 0.652
2.586GlyMet: 2.586 ± 0.306
3.664GlyAsn: 3.664 ± 0.361
3.103GlyPro: 3.103 ± 0.326
2.888GlyGln: 2.888 ± 0.286
3.448GlyArg: 3.448 ± 0.364
5.043GlySer: 5.043 ± 0.571
5.862GlyThr: 5.862 ± 0.657
4.569GlyVal: 4.569 ± 0.511
1.422GlyTrp: 1.422 ± 0.295
2.802GlyTyr: 2.802 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
1.293HisAla: 1.293 ± 0.234
0.172HisCys: 0.172 ± 0.086
1.422HisAsp: 1.422 ± 0.288
1.25HisGlu: 1.25 ± 0.24
0.517HisPhe: 0.517 ± 0.164
1.164HisGly: 1.164 ± 0.228
0.216HisHis: 0.216 ± 0.096
0.776HisIle: 0.776 ± 0.204
1.121HisLys: 1.121 ± 0.25
1.034HisLeu: 1.034 ± 0.279
0.56HisMet: 0.56 ± 0.143
0.69HisAsn: 0.69 ± 0.163
1.293HisPro: 1.293 ± 0.269
0.56HisGln: 0.56 ± 0.113
1.034HisArg: 1.034 ± 0.287
0.948HisSer: 0.948 ± 0.23
0.991HisThr: 0.991 ± 0.174
1.078HisVal: 1.078 ± 0.217
0.388HisTrp: 0.388 ± 0.116
0.431HisTyr: 0.431 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.0IleAla: 5.0 ± 0.433
0.733IleCys: 0.733 ± 0.25
3.405IleAsp: 3.405 ± 0.331
3.793IleGlu: 3.793 ± 0.348
2.112IlePhe: 2.112 ± 0.301
4.052IleGly: 4.052 ± 0.801
1.25IleHis: 1.25 ± 0.263
3.276IleIle: 3.276 ± 0.311
3.405IleLys: 3.405 ± 0.327
4.483IleLeu: 4.483 ± 0.336
1.422IleMet: 1.422 ± 0.282
2.888IleAsn: 2.888 ± 0.292
3.362IlePro: 3.362 ± 0.411
2.241IleGln: 2.241 ± 0.282
3.19IleArg: 3.19 ± 0.405
3.966IleSer: 3.966 ± 0.449
3.836IleThr: 3.836 ± 0.424
3.233IleVal: 3.233 ± 0.334
0.991IleTrp: 0.991 ± 0.195
1.681IleTyr: 1.681 ± 0.407
0.0IleXaa: 0.0 ± 0.0
Lys
5.129LysAla: 5.129 ± 1.028
0.474LysCys: 0.474 ± 0.215
3.147LysAsp: 3.147 ± 0.563
2.5LysGlu: 2.5 ± 0.315
3.19LysPhe: 3.19 ± 0.359
3.491LysGly: 3.491 ± 0.483
1.552LysHis: 1.552 ± 0.299
3.319LysIle: 3.319 ± 0.413
4.181LysLys: 4.181 ± 0.546
4.181LysLeu: 4.181 ± 0.408
1.94LysMet: 1.94 ± 0.296
3.19LysAsn: 3.19 ± 0.308
2.155LysPro: 2.155 ± 0.376
2.457LysGln: 2.457 ± 0.304
3.578LysArg: 3.578 ± 0.468
3.664LysSer: 3.664 ± 0.328
3.233LysThr: 3.233 ± 0.352
3.534LysVal: 3.534 ± 0.391
1.164LysTrp: 1.164 ± 0.196
1.509LysTyr: 1.509 ± 0.331
0.0LysXaa: 0.0 ± 0.0
Leu
6.509LeuAla: 6.509 ± 0.612
0.905LeuCys: 0.905 ± 0.23
5.345LeuAsp: 5.345 ± 0.444
5.345LeuGlu: 5.345 ± 0.542
2.716LeuPhe: 2.716 ± 0.332
5.948LeuGly: 5.948 ± 0.61
0.905LeuHis: 0.905 ± 0.232
4.052LeuIle: 4.052 ± 0.415
3.793LeuLys: 3.793 ± 0.47
4.914LeuLeu: 4.914 ± 0.53
1.767LeuMet: 1.767 ± 0.268
4.009LeuAsn: 4.009 ± 0.479
4.224LeuPro: 4.224 ± 0.413
2.414LeuGln: 2.414 ± 0.317
3.19LeuArg: 3.19 ± 0.447
5.086LeuSer: 5.086 ± 0.434
4.784LeuThr: 4.784 ± 0.446
4.828LeuVal: 4.828 ± 0.516
0.776LeuTrp: 0.776 ± 0.152
1.853LeuTyr: 1.853 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
3.06MetAla: 3.06 ± 0.333
0.388MetCys: 0.388 ± 0.152
1.638MetAsp: 1.638 ± 0.275
1.509MetGlu: 1.509 ± 0.282
0.776MetPhe: 0.776 ± 0.172
2.112MetGly: 2.112 ± 0.348
0.474MetHis: 0.474 ± 0.16
1.422MetIle: 1.422 ± 0.22
1.595MetLys: 1.595 ± 0.333
2.371MetLeu: 2.371 ± 0.336
0.647MetMet: 0.647 ± 0.175
1.595MetAsn: 1.595 ± 0.265
1.164MetPro: 1.164 ± 0.244
0.905MetGln: 0.905 ± 0.212
1.681MetArg: 1.681 ± 0.282
2.371MetSer: 2.371 ± 0.227
1.983MetThr: 1.983 ± 0.3
1.81MetVal: 1.81 ± 0.283
0.302MetTrp: 0.302 ± 0.127
0.302MetTyr: 0.302 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
4.483AsnAla: 4.483 ± 0.846
0.345AsnCys: 0.345 ± 0.192
3.103AsnAsp: 3.103 ± 0.333
2.759AsnGlu: 2.759 ± 0.38
2.198AsnPhe: 2.198 ± 0.38
4.267AsnGly: 4.267 ± 0.399
0.948AsnHis: 0.948 ± 0.227
2.716AsnIle: 2.716 ± 0.37
2.974AsnLys: 2.974 ± 0.393
3.448AsnLeu: 3.448 ± 0.512
1.595AsnMet: 1.595 ± 0.294
2.284AsnAsn: 2.284 ± 0.351
2.802AsnPro: 2.802 ± 0.254
1.983AsnGln: 1.983 ± 0.267
2.457AsnArg: 2.457 ± 0.283
3.707AsnSer: 3.707 ± 0.452
3.017AsnThr: 3.017 ± 0.403
2.845AsnVal: 2.845 ± 0.323
0.431AsnTrp: 0.431 ± 0.123
1.466AsnTyr: 1.466 ± 0.242
0.0AsnXaa: 0.0 ± 0.0
Pro
4.741ProAla: 4.741 ± 0.558
0.474ProCys: 0.474 ± 0.176
3.233ProAsp: 3.233 ± 0.481
3.75ProGlu: 3.75 ± 0.51
1.552ProPhe: 1.552 ± 0.264
4.31ProGly: 4.31 ± 0.445
0.862ProHis: 0.862 ± 0.213
2.543ProIle: 2.543 ± 0.343
2.543ProLys: 2.543 ± 0.333
2.845ProLeu: 2.845 ± 0.336
1.164ProMet: 1.164 ± 0.188
2.241ProAsn: 2.241 ± 0.327
2.112ProPro: 2.112 ± 0.49
1.767ProGln: 1.767 ± 0.254
2.026ProArg: 2.026 ± 0.344
2.802ProSer: 2.802 ± 0.466
2.371ProThr: 2.371 ± 0.319
4.138ProVal: 4.138 ± 0.678
0.647ProTrp: 0.647 ± 0.192
1.164ProTyr: 1.164 ± 0.183
0.0ProXaa: 0.0 ± 0.0
Gln
4.138GlnAla: 4.138 ± 0.618
0.431GlnCys: 0.431 ± 0.169
2.629GlnAsp: 2.629 ± 0.677
2.759GlnGlu: 2.759 ± 0.337
1.983GlnPhe: 1.983 ± 0.289
2.328GlnGly: 2.328 ± 0.282
0.388GlnHis: 0.388 ± 0.137
2.198GlnIle: 2.198 ± 0.366
2.241GlnLys: 2.241 ± 0.289
2.802GlnLeu: 2.802 ± 0.384
1.422GlnMet: 1.422 ± 0.266
1.81GlnAsn: 1.81 ± 0.254
1.81GlnPro: 1.81 ± 0.426
1.595GlnGln: 1.595 ± 0.392
1.94GlnArg: 1.94 ± 0.241
1.552GlnSer: 1.552 ± 0.266
1.767GlnThr: 1.767 ± 0.439
2.586GlnVal: 2.586 ± 0.306
0.56GlnTrp: 0.56 ± 0.132
1.595GlnTyr: 1.595 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
4.784ArgAla: 4.784 ± 0.535
0.474ArgCys: 0.474 ± 0.132
3.405ArgAsp: 3.405 ± 0.44
3.233ArgGlu: 3.233 ± 0.408
1.94ArgPhe: 1.94 ± 0.309
3.19ArgGly: 3.19 ± 0.391
0.862ArgHis: 0.862 ± 0.178
3.448ArgIle: 3.448 ± 0.42
3.879ArgLys: 3.879 ± 0.574
3.319ArgLeu: 3.319 ± 0.374
1.681ArgMet: 1.681 ± 0.353
2.543ArgAsn: 2.543 ± 0.42
2.284ArgPro: 2.284 ± 0.345
1.466ArgGln: 1.466 ± 0.193
2.845ArgArg: 2.845 ± 0.284
2.888ArgSer: 2.888 ± 0.311
2.328ArgThr: 2.328 ± 0.438
3.621ArgVal: 3.621 ± 0.35
0.69ArgTrp: 0.69 ± 0.194
1.897ArgTyr: 1.897 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
6.379SerAla: 6.379 ± 0.586
0.819SerCys: 0.819 ± 0.223
4.483SerAsp: 4.483 ± 0.585
3.707SerGlu: 3.707 ± 0.622
2.371SerPhe: 2.371 ± 0.339
6.853SerGly: 6.853 ± 0.737
0.862SerHis: 0.862 ± 0.172
3.362SerIle: 3.362 ± 0.416
3.707SerLys: 3.707 ± 0.425
5.345SerLeu: 5.345 ± 0.474
1.897SerMet: 1.897 ± 0.285
2.457SerAsn: 2.457 ± 0.389
2.328SerPro: 2.328 ± 0.399
3.103SerGln: 3.103 ± 0.394
3.491SerArg: 3.491 ± 0.35
3.534SerSer: 3.534 ± 0.532
3.621SerThr: 3.621 ± 0.46
4.612SerVal: 4.612 ± 0.4
0.862SerTrp: 0.862 ± 0.188
2.241SerTyr: 2.241 ± 0.31
0.0SerXaa: 0.0 ± 0.0
Thr
6.293ThrAla: 6.293 ± 1.082
0.431ThrCys: 0.431 ± 0.167
3.664ThrAsp: 3.664 ± 0.509
4.31ThrGlu: 4.31 ± 0.413
1.724ThrPhe: 1.724 ± 0.281
5.474ThrGly: 5.474 ± 0.597
0.776ThrHis: 0.776 ± 0.195
2.5ThrIle: 2.5 ± 0.32
2.716ThrLys: 2.716 ± 0.362
3.707ThrLeu: 3.707 ± 0.341
1.336ThrMet: 1.336 ± 0.254
2.716ThrAsn: 2.716 ± 0.406
2.888ThrPro: 2.888 ± 0.308
2.672ThrGln: 2.672 ± 0.234
2.759ThrArg: 2.759 ± 0.349
3.578ThrSer: 3.578 ± 0.379
2.845ThrThr: 2.845 ± 0.44
4.698ThrVal: 4.698 ± 0.499
0.733ThrTrp: 0.733 ± 0.219
1.595ThrTyr: 1.595 ± 0.182
0.0ThrXaa: 0.0 ± 0.0
Val
5.043ValAla: 5.043 ± 0.674
0.603ValCys: 0.603 ± 0.178
4.569ValAsp: 4.569 ± 0.469
4.655ValGlu: 4.655 ± 0.503
2.845ValPhe: 2.845 ± 0.362
4.267ValGly: 4.267 ± 0.591
1.121ValHis: 1.121 ± 0.233
4.224ValIle: 4.224 ± 0.391
2.672ValLys: 2.672 ± 0.349
5.388ValLeu: 5.388 ± 0.544
1.681ValMet: 1.681 ± 0.231
3.534ValAsn: 3.534 ± 0.32
2.974ValPro: 2.974 ± 0.386
2.241ValGln: 2.241 ± 0.332
3.103ValArg: 3.103 ± 0.349
5.0ValSer: 5.0 ± 0.492
4.009ValThr: 4.009 ± 0.367
4.353ValVal: 4.353 ± 0.335
0.819ValTrp: 0.819 ± 0.193
2.371ValTyr: 2.371 ± 0.364
0.0ValXaa: 0.0 ± 0.0
Trp
1.25TrpAla: 1.25 ± 0.204
0.259TrpCys: 0.259 ± 0.105
1.25TrpAsp: 1.25 ± 0.191
0.819TrpGlu: 0.819 ± 0.187
0.776TrpPhe: 0.776 ± 0.228
1.078TrpGly: 1.078 ± 0.207
0.216TrpHis: 0.216 ± 0.113
1.078TrpIle: 1.078 ± 0.302
0.603TrpLys: 0.603 ± 0.153
1.121TrpLeu: 1.121 ± 0.211
0.388TrpMet: 0.388 ± 0.113
0.991TrpAsn: 0.991 ± 0.138
0.345TrpPro: 0.345 ± 0.133
0.603TrpGln: 0.603 ± 0.154
1.078TrpArg: 1.078 ± 0.187
1.207TrpSer: 1.207 ± 0.182
0.56TrpThr: 0.56 ± 0.148
0.69TrpVal: 0.69 ± 0.145
0.0TrpTrp: 0.0 ± 0.0
0.647TrpTyr: 0.647 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.026TyrAla: 2.026 ± 0.284
0.345TyrCys: 0.345 ± 0.132
2.069TyrAsp: 2.069 ± 0.285
1.681TyrGlu: 1.681 ± 0.249
0.991TyrPhe: 0.991 ± 0.178
3.103TyrGly: 3.103 ± 0.373
0.345TyrHis: 0.345 ± 0.126
1.983TyrIle: 1.983 ± 0.324
1.767TyrLys: 1.767 ± 0.237
2.457TyrLeu: 2.457 ± 0.36
0.69TyrMet: 0.69 ± 0.169
1.078TyrAsn: 1.078 ± 0.223
0.991TyrPro: 0.991 ± 0.265
1.121TyrGln: 1.121 ± 0.255
2.328TyrArg: 2.328 ± 0.354
1.81TyrSer: 1.81 ± 0.407
1.724TyrThr: 1.724 ± 0.337
1.94TyrVal: 1.94 ± 0.31
0.388TyrTrp: 0.388 ± 0.113
0.862TyrTyr: 0.862 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (23201 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski