Amino acid dipepetide frequency for Streptomyces varsoviensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.798AlaAla: 22.798 ± 0.147	1.13AlaCys: 1.13 ± 0.027	8.933AlaAsp: 8.933 ± 0.075	9.446AlaGlu: 9.446 ± 0.094	3.642AlaPhe: 3.642 ± 0.042	13.474AlaGly: 13.474 ± 0.098	3.028AlaHis: 3.028 ± 0.044	3.57AlaIle: 3.57 ± 0.048	3.139AlaLys: 3.139 ± 0.054	14.788AlaLeu: 14.788 ± 0.119	2.664AlaMet: 2.664 ± 0.036	1.975AlaAsn: 1.975 ± 0.039	7.599AlaPro: 7.599 ± 0.064	3.622AlaGln: 3.622 ± 0.051	10.996AlaArg: 10.996 ± 0.087	5.708AlaSer: 5.708 ± 0.058	6.769AlaThr: 6.769 ± 0.066	12.142AlaVal: 12.142 ± 0.086	1.856AlaTrp: 1.856 ± 0.028	2.904AlaTyr: 2.904 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.214CysAla: 1.214 ± 0.029	0.097CysCys: 0.097 ± 0.007	0.497CysAsp: 0.497 ± 0.016	0.415CysGlu: 0.415 ± 0.014	0.24CysPhe: 0.24 ± 0.01	0.964CysGly: 0.964 ± 0.023	0.19CysHis: 0.19 ± 0.01	0.184CysIle: 0.184 ± 0.01	0.115CysLys: 0.115 ± 0.008	0.798CysLeu: 0.798 ± 0.023	0.139CysMet: 0.139 ± 0.008	0.117CysAsn: 0.117 ± 0.009	0.497CysPro: 0.497 ± 0.015	0.174CysGln: 0.174 ± 0.01	0.62CysArg: 0.62 ± 0.018	0.441CysSer: 0.441 ± 0.014	0.463CysThr: 0.463 ± 0.016	0.723CysVal: 0.723 ± 0.019	0.117CysTrp: 0.117 ± 0.009	0.146CysTyr: 0.146 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.297AspAla: 8.297 ± 0.083	0.417AspCys: 0.417 ± 0.016	3.527AspAsp: 3.527 ± 0.048	3.825AspGlu: 3.825 ± 0.05	1.628AspPhe: 1.628 ± 0.032	6.564AspGly: 6.564 ± 0.072	1.499AspHis: 1.499 ± 0.029	2.013AspIle: 2.013 ± 0.034	1.26AspLys: 1.26 ± 0.03	6.112AspLeu: 6.112 ± 0.062	0.815AspMet: 0.815 ± 0.017	0.897AspAsn: 0.897 ± 0.023	4.355AspPro: 4.355 ± 0.043	1.561AspGln: 1.561 ± 0.029	5.0AspArg: 5.0 ± 0.056	2.635AspSer: 2.635 ± 0.035	2.935AspThr: 2.935 ± 0.045	4.5AspVal: 4.5 ± 0.051	1.029AspTrp: 1.029 ± 0.026	1.159AspTyr: 1.159 ± 0.028	0.0AspXaa: 0.0 ± 0.0
Glu	7.659GluAla: 7.659 ± 0.074	0.39GluCys: 0.39 ± 0.016	2.808GluAsp: 2.808 ± 0.04	3.561GluGlu: 3.561 ± 0.048	1.583GluPhe: 1.583 ± 0.031	4.465GluGly: 4.465 ± 0.057	1.617GluHis: 1.617 ± 0.026	2.257GluIle: 2.257 ± 0.035	1.378GluLys: 1.378 ± 0.03	7.04GluLeu: 7.04 ± 0.068	0.915GluMet: 0.915 ± 0.02	1.065GluAsn: 1.065 ± 0.025	3.416GluPro: 3.416 ± 0.049	2.072GluGln: 2.072 ± 0.034	5.943GluArg: 5.943 ± 0.061	2.623GluSer: 2.623 ± 0.041	2.873GluThr: 2.873 ± 0.036	4.303GluVal: 4.303 ± 0.057	0.758GluTrp: 0.758 ± 0.019	1.135GluTyr: 1.135 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.828PheAla: 3.828 ± 0.044	0.273PheCys: 0.273 ± 0.013	2.021PheAsp: 2.021 ± 0.034	1.492PheGlu: 1.492 ± 0.032	0.903PhePhe: 0.903 ± 0.023	3.099PheGly: 3.099 ± 0.05	0.63PheHis: 0.63 ± 0.017	0.794PheIle: 0.794 ± 0.021	0.538PheLys: 0.538 ± 0.017	2.583PheLeu: 2.583 ± 0.04	0.395PheMet: 0.395 ± 0.013	0.56PheAsn: 0.56 ± 0.018	1.45PhePro: 1.45 ± 0.028	0.673PheGln: 0.673 ± 0.02	1.784PheArg: 1.784 ± 0.028	1.452PheSer: 1.452 ± 0.026	1.961PheThr: 1.961 ± 0.032	2.113PheVal: 2.113 ± 0.034	0.411PheTrp: 0.411 ± 0.016	0.581PheTyr: 0.581 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	12.087GlyAla: 12.087 ± 0.107	0.841GlyCys: 0.841 ± 0.022	5.283GlyAsp: 5.283 ± 0.057	5.355GlyGlu: 5.355 ± 0.063	2.857GlyPhe: 2.857 ± 0.041	9.347GlyGly: 9.347 ± 0.095	2.403GlyHis: 2.403 ± 0.037	3.278GlyIle: 3.278 ± 0.044	2.559GlyLys: 2.559 ± 0.046	9.535GlyLeu: 9.535 ± 0.081	2.08GlyMet: 2.08 ± 0.034	1.6GlyAsn: 1.6 ± 0.032	5.566GlyPro: 5.566 ± 0.066	2.66GlyGln: 2.66 ± 0.039	7.992GlyArg: 7.992 ± 0.07	5.159GlySer: 5.159 ± 0.053	5.883GlyThr: 5.883 ± 0.066	7.57GlyVal: 7.57 ± 0.08	1.644GlyTrp: 1.644 ± 0.031	2.268GlyTyr: 2.268 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.799HisAla: 2.799 ± 0.037	0.216HisCys: 0.216 ± 0.012	1.338HisAsp: 1.338 ± 0.029	1.217HisGlu: 1.217 ± 0.027	0.669HisPhe: 0.669 ± 0.019	2.492HisGly: 2.492 ± 0.042	0.734HisHis: 0.734 ± 0.022	0.78HisIle: 0.78 ± 0.021	0.374HisLys: 0.374 ± 0.015	2.451HisLeu: 2.451 ± 0.042	0.384HisMet: 0.384 ± 0.014	0.373HisAsn: 0.373 ± 0.012	1.854HisPro: 1.854 ± 0.034	0.708HisGln: 0.708 ± 0.019	2.079HisArg: 2.079 ± 0.037	1.077HisSer: 1.077 ± 0.023	1.318HisThr: 1.318 ± 0.026	1.655HisVal: 1.655 ± 0.029	0.38HisTrp: 0.38 ± 0.015	0.517HisTyr: 0.517 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.938IleAla: 4.938 ± 0.053	0.314IleCys: 0.314 ± 0.013	2.275IleAsp: 2.275 ± 0.035	2.068IleGlu: 2.068 ± 0.038	0.718IlePhe: 0.718 ± 0.019	3.61IleGly: 3.61 ± 0.048	0.668IleHis: 0.668 ± 0.019	0.901IleIle: 0.901 ± 0.024	0.802IleLys: 0.802 ± 0.023	2.266IleLeu: 2.266 ± 0.04	0.472IleMet: 0.472 ± 0.014	0.699IleAsn: 0.699 ± 0.019	1.679IlePro: 1.679 ± 0.029	0.684IleGln: 0.684 ± 0.017	2.183IleArg: 2.183 ± 0.037	1.729IleSer: 1.729 ± 0.033	2.181IleThr: 2.181 ± 0.03	2.699IleVal: 2.699 ± 0.04	0.353IleTrp: 0.353 ± 0.015	0.532IleTyr: 0.532 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.974LysAla: 2.974 ± 0.052	0.129LysCys: 0.129 ± 0.008	1.374LysAsp: 1.374 ± 0.032	1.243LysGlu: 1.243 ± 0.03	0.48LysPhe: 0.48 ± 0.019	1.947LysGly: 1.947 ± 0.033	0.448LysHis: 0.448 ± 0.017	0.914LysIle: 0.914 ± 0.025	0.965LysLys: 0.965 ± 0.032	2.102LysLeu: 2.102 ± 0.038	0.385LysMet: 0.385 ± 0.014	0.59LysAsn: 0.59 ± 0.02	1.326LysPro: 1.326 ± 0.031	0.696LysGln: 0.696 ± 0.019	1.59LysArg: 1.59 ± 0.029	1.217LysSer: 1.217 ± 0.028	1.322LysThr: 1.322 ± 0.028	1.84LysVal: 1.84 ± 0.035	0.277LysTrp: 0.277 ± 0.013	0.432LysTyr: 0.432 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.339LeuAla: 15.339 ± 0.118	0.881LeuCys: 0.881 ± 0.024	6.814LeuAsp: 6.814 ± 0.06	4.58LeuGlu: 4.58 ± 0.054	2.675LeuPhe: 2.675 ± 0.042	9.293LeuGly: 9.293 ± 0.083	2.304LeuHis: 2.304 ± 0.034	3.414LeuIle: 3.414 ± 0.055	1.951LeuLys: 1.951 ± 0.035	11.237LeuLeu: 11.237 ± 0.117	1.731LeuMet: 1.731 ± 0.03	1.65LeuAsn: 1.65 ± 0.034	6.427LeuPro: 6.427 ± 0.054	2.067LeuGln: 2.067 ± 0.031	8.964LeuArg: 8.964 ± 0.079	5.313LeuSer: 5.313 ± 0.053	6.634LeuThr: 6.634 ± 0.058	8.445LeuVal: 8.445 ± 0.073	1.229LeuTrp: 1.229 ± 0.03	1.855LeuTyr: 1.855 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.456MetAla: 2.456 ± 0.031	0.181MetCys: 0.181 ± 0.008	0.938MetAsp: 0.938 ± 0.019	0.789MetGlu: 0.789 ± 0.022	0.462MetPhe: 0.462 ± 0.016	1.369MetGly: 1.369 ± 0.027	0.381MetHis: 0.381 ± 0.013	0.674MetIle: 0.674 ± 0.019	0.426MetLys: 0.426 ± 0.015	1.745MetLeu: 1.745 ± 0.031	0.316MetMet: 0.316 ± 0.013	0.429MetAsn: 0.429 ± 0.014	1.129MetPro: 1.129 ± 0.024	0.423MetGln: 0.423 ± 0.016	1.503MetArg: 1.503 ± 0.025	1.288MetSer: 1.288 ± 0.027	1.462MetThr: 1.462 ± 0.028	1.309MetVal: 1.309 ± 0.029	0.224MetTrp: 0.224 ± 0.012	0.351MetTyr: 0.351 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.286AsnAla: 2.286 ± 0.034	0.164AsnCys: 0.164 ± 0.01	0.917AsnAsp: 0.917 ± 0.024	0.779AsnGlu: 0.779 ± 0.025	0.483AsnPhe: 0.483 ± 0.016	1.801AsnGly: 1.801 ± 0.035	0.374AsnHis: 0.374 ± 0.014	0.624AsnIle: 0.624 ± 0.018	0.439AsnLys: 0.439 ± 0.019	1.608AsnLeu: 1.608 ± 0.03	0.301AsnMet: 0.301 ± 0.012	0.38AsnAsn: 0.38 ± 0.015	1.284AsnPro: 1.284 ± 0.028	0.481AsnGln: 0.481 ± 0.017	1.241AsnArg: 1.241 ± 0.032	0.906AsnSer: 0.906 ± 0.022	1.06AsnThr: 1.06 ± 0.023	1.354AsnVal: 1.354 ± 0.03	0.283AsnTrp: 0.283 ± 0.013	0.427AsnTyr: 0.427 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	8.827ProAla: 8.827 ± 0.073	0.344ProCys: 0.344 ± 0.013	4.507ProAsp: 4.507 ± 0.06	4.467ProGlu: 4.467 ± 0.053	1.528ProPhe: 1.528 ± 0.03	6.782ProGly: 6.782 ± 0.066	1.413ProHis: 1.413 ± 0.032	1.363ProIle: 1.363 ± 0.026	1.306ProLys: 1.306 ± 0.026	5.364ProLeu: 5.364 ± 0.053	1.054ProMet: 1.054 ± 0.024	0.9ProAsn: 0.9 ± 0.023	3.483ProPro: 3.483 ± 0.051	1.552ProGln: 1.552 ± 0.028	4.257ProArg: 4.257 ± 0.052	3.063ProSer: 3.063 ± 0.041	3.035ProThr: 3.035 ± 0.044	5.246ProVal: 5.246 ± 0.057	0.909ProTrp: 0.909 ± 0.022	1.498ProTyr: 1.498 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.515GlnAla: 3.515 ± 0.041	0.177GlnCys: 0.177 ± 0.009	1.313GlnAsp: 1.313 ± 0.028	1.398GlnGlu: 1.398 ± 0.027	0.695GlnPhe: 0.695 ± 0.02	2.266GlnGly: 2.266 ± 0.038	0.682GlnHis: 0.682 ± 0.016	1.012GlnIle: 1.012 ± 0.025	0.581GlnLys: 0.581 ± 0.018	2.902GlnLeu: 2.902 ± 0.039	0.487GlnMet: 0.487 ± 0.015	0.486GlnAsn: 0.486 ± 0.018	1.635GlnPro: 1.635 ± 0.031	1.107GlnGln: 1.107 ± 0.028	2.472GlnArg: 2.472 ± 0.033	1.239GlnSer: 1.239 ± 0.025	1.241GlnThr: 1.241 ± 0.026	2.105GlnVal: 2.105 ± 0.035	0.452GlnTrp: 0.452 ± 0.016	0.579GlnTyr: 0.579 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.64ArgAla: 10.64 ± 0.098	0.64ArgCys: 0.64 ± 0.021	4.435ArgAsp: 4.435 ± 0.053	4.977ArgGlu: 4.977 ± 0.059	2.372ArgPhe: 2.372 ± 0.037	6.336ArgGly: 6.336 ± 0.065	2.13ArgHis: 2.13 ± 0.036	2.956ArgIle: 2.956 ± 0.038	1.677ArgLys: 1.677 ± 0.035	8.871ArgLeu: 8.871 ± 0.074	1.659ArgMet: 1.659 ± 0.029	1.3ArgAsn: 1.3 ± 0.025	5.192ArgPro: 5.192 ± 0.057	2.336ArgGln: 2.336 ± 0.036	7.802ArgArg: 7.802 ± 0.078	4.013ArgSer: 4.013 ± 0.045	5.483ArgThr: 5.483 ± 0.053	6.165ArgVal: 6.165 ± 0.051	1.356ArgTrp: 1.356 ± 0.028	1.916ArgTyr: 1.916 ± 0.031	0.001ArgXaa: 0.001 ± 0.0
Ser	6.768SerAla: 6.768 ± 0.069	0.417SerCys: 0.417 ± 0.013	2.638SerAsp: 2.638 ± 0.039	2.387SerGlu: 2.387 ± 0.033	1.554SerPhe: 1.554 ± 0.031	5.857SerGly: 5.857 ± 0.059	1.009SerHis: 1.009 ± 0.027	1.436SerIle: 1.436 ± 0.031	1.057SerLys: 1.057 ± 0.024	4.794SerLeu: 4.794 ± 0.054	1.085SerMet: 1.085 ± 0.024	0.836SerAsn: 0.836 ± 0.021	3.138SerPro: 3.138 ± 0.042	1.165SerGln: 1.165 ± 0.024	3.533SerArg: 3.533 ± 0.044	2.689SerSer: 2.689 ± 0.051	2.879SerThr: 2.879 ± 0.046	4.154SerVal: 4.154 ± 0.045	0.843SerTrp: 0.843 ± 0.021	1.232SerTyr: 1.232 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.657ThrAla: 8.657 ± 0.071	0.402ThrCys: 0.402 ± 0.016	3.389ThrAsp: 3.389 ± 0.045	3.162ThrGlu: 3.162 ± 0.041	1.48ThrPhe: 1.48 ± 0.033	6.475ThrGly: 6.475 ± 0.068	1.192ThrHis: 1.192 ± 0.022	1.704ThrIle: 1.704 ± 0.029	1.164ThrLys: 1.164 ± 0.028	5.435ThrLeu: 5.435 ± 0.057	1.002ThrMet: 1.002 ± 0.023	0.944ThrAsn: 0.944 ± 0.024	3.9ThrPro: 3.9 ± 0.045	1.183ThrGln: 1.183 ± 0.024	3.702ThrArg: 3.702 ± 0.043	2.959ThrSer: 2.959 ± 0.04	3.385ThrThr: 3.385 ± 0.045	5.806ThrVal: 5.806 ± 0.061	0.826ThrTrp: 0.826 ± 0.022	1.277ThrTyr: 1.277 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.591ValAla: 10.591 ± 0.079	0.783ValCys: 0.783 ± 0.019	4.832ValAsp: 4.832 ± 0.049	4.664ValGlu: 4.664 ± 0.048	2.417ValPhe: 2.417 ± 0.036	6.386ValGly: 6.386 ± 0.063	1.923ValHis: 1.923 ± 0.033	2.872ValIle: 2.872 ± 0.041	1.715ValLys: 1.715 ± 0.031	9.286ValLeu: 9.286 ± 0.088	1.411ValMet: 1.411 ± 0.029	1.63ValAsn: 1.63 ± 0.034	5.073ValPro: 5.073 ± 0.053	1.972ValGln: 1.972 ± 0.032	7.438ValArg: 7.438 ± 0.077	4.158ValSer: 4.158 ± 0.049	5.011ValThr: 5.011 ± 0.05	7.428ValVal: 7.428 ± 0.078	1.061ValTrp: 1.061 ± 0.024	1.528ValTyr: 1.528 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.66TrpAla: 1.66 ± 0.028	0.156TrpCys: 0.156 ± 0.009	0.779TrpAsp: 0.779 ± 0.022	0.721TrpGlu: 0.721 ± 0.016	0.496TrpPhe: 0.496 ± 0.016	1.083TrpGly: 1.083 ± 0.023	0.381TrpHis: 0.381 ± 0.013	0.505TrpIle: 0.505 ± 0.016	0.363TrpLys: 0.363 ± 0.013	1.737TrpLeu: 1.737 ± 0.031	0.283TrpMet: 0.283 ± 0.015	0.377TrpAsn: 0.377 ± 0.017	0.803TrpPro: 0.803 ± 0.019	0.596TrpGln: 0.596 ± 0.02	1.325TrpArg: 1.325 ± 0.024	0.899TrpSer: 0.899 ± 0.02	0.943TrpThr: 0.943 ± 0.026	0.926TrpVal: 0.926 ± 0.021	0.307TrpTrp: 0.307 ± 0.013	0.333TrpTyr: 0.333 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.85TyrAla: 2.85 ± 0.038	0.197TyrCys: 0.197 ± 0.01	1.407TyrAsp: 1.407 ± 0.027	1.315TyrGlu: 1.315 ± 0.026	0.657TyrPhe: 0.657 ± 0.017	2.356TyrGly: 2.356 ± 0.032	0.411TyrHis: 0.411 ± 0.014	0.52TyrIle: 0.52 ± 0.016	0.442TyrLys: 0.442 ± 0.015	2.152TyrLeu: 2.152 ± 0.036	0.274TyrMet: 0.274 ± 0.012	0.386TyrAsn: 0.386 ± 0.014	1.091TyrPro: 1.091 ± 0.021	0.611TyrGln: 0.611 ± 0.018	1.833TyrArg: 1.833 ± 0.032	0.904TyrSer: 0.904 ± 0.024	1.207TyrThr: 1.207 ± 0.03	1.698TyrVal: 1.698 ± 0.031	0.358TyrTrp: 0.358 ± 0.013	0.447TyrTyr: 0.447 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8643 proteins (1977507 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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