Scophthalmus maximus rhabdovirus
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7D0U3|E7D0U3_9RHAB C protein OS=Scophthalmus maximus rhabdovirus OX=936149 GN=C PE=4 SV=1
MM1 pKa = 7.29 SHH3 pKa = 6.7 GLSKK7 pKa = 10.88 SRR9 pKa = 11.84 TFEE12 pKa = 4.28 EE13 pKa = 4.11 ITDD16 pKa = 3.77 VEE18 pKa = 4.29 QVLDD22 pKa = 4.3 NIRR25 pKa = 11.84 QTEE28 pKa = 4.38 SKK30 pKa = 10.51 LGTSAGAGASDD41 pKa = 3.8 EE42 pKa = 4.63 PYY44 pKa = 10.15 PAGGDD49 pKa = 3.62 LPPASGGFRR58 pKa = 11.84 YY59 pKa = 10.0 LSSQEE64 pKa = 3.69 EE65 pKa = 4.32 GSYY68 pKa = 10.5 KK69 pKa = 10.45 EE70 pKa = 5.82 EE71 pKa = 3.86 EE72 pKa = 4.54 DD73 pKa = 3.08 NWLINQSRR81 pKa = 11.84 AAKK84 pKa = 9.91 LVAPLADD91 pKa = 3.52 YY92 pKa = 10.87 RR93 pKa = 11.84 LAAAADD99 pKa = 4.1 KK100 pKa = 10.98 DD101 pKa = 4.03 PSPEE105 pKa = 4.17 PEE107 pKa = 3.86 DD108 pKa = 3.22 QRR110 pKa = 11.84 RR111 pKa = 11.84 EE112 pKa = 3.8 IEE114 pKa = 3.92 EE115 pKa = 4.64 RR116 pKa = 11.84 IAEE119 pKa = 4.21 AHH121 pKa = 5.98 DD122 pKa = 3.5 QMLKK126 pKa = 10.82 FEE128 pKa = 4.79 MFPYY132 pKa = 9.84 TYY134 pKa = 10.93 FMPDD138 pKa = 3.23 YY139 pKa = 10.37 LKK141 pKa = 10.99 SEE143 pKa = 4.26 DD144 pKa = 3.72 QQEE147 pKa = 4.25 LVHH150 pKa = 6.81 FLTEE154 pKa = 3.71 ILGPRR159 pKa = 11.84 NSNRR163 pKa = 11.84 DD164 pKa = 2.94 FWIRR168 pKa = 11.84 GTHH171 pKa = 5.82 IPTMCWRR178 pKa = 11.84 NGDD181 pKa = 3.41 MHH183 pKa = 7.82 IQDD186 pKa = 4.4 DD187 pKa = 4.58 VEE189 pKa = 4.68 SKK191 pKa = 10.89 SSGVSGLTKK200 pKa = 10.37 ALKK203 pKa = 10.05 SVQFVDD209 pKa = 4.53 PGEE212 pKa = 4.05 QDD214 pKa = 3.24 SATVEE219 pKa = 4.19 SEE221 pKa = 3.42 MDD223 pKa = 3.73 RR224 pKa = 11.84 DD225 pKa = 3.8 LDD227 pKa = 4.04 CYY229 pKa = 11.19 SIGEE233 pKa = 4.19 SSLVSEE239 pKa = 5.05 FMLDD243 pKa = 3.22 TSDD246 pKa = 5.62 LMEE249 pKa = 4.49 SLNRR253 pKa = 11.84 EE254 pKa = 3.82 WLVATKK260 pKa = 10.24 KK261 pKa = 10.8 GRR263 pKa = 11.84 WKK265 pKa = 9.53 TLGPLFLRR273 pKa = 11.84 PLAQDD278 pKa = 3.26 RR279 pKa = 11.84 ALMKK283 pKa = 10.28 TILSEE288 pKa = 3.98 MRR290 pKa = 11.84 EE291 pKa = 3.95 KK292 pKa = 10.93 DD293 pKa = 3.15 VTEE296 pKa = 4.08 QMSCLLTSNEE306 pKa = 3.8 LTSRR310 pKa = 11.84 FVRR313 pKa = 11.84 TLDD316 pKa = 4.1 LGTLKK321 pKa = 10.44 MKK323 pKa = 10.85 GSFF326 pKa = 3.44
Molecular weight: 36.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.741
IPC2_protein 4.673
IPC_protein 4.609
Toseland 4.457
ProMoST 4.711
Dawson 4.558
Bjellqvist 4.698
Wikipedia 4.418
Rodwell 4.457
Grimsley 4.368
Solomon 4.558
Lehninger 4.507
Nozaki 4.66
DTASelect 4.813
Thurlkill 4.457
EMBOSS 4.444
Sillero 4.724
Patrickios 4.126
IPC_peptide 4.558
IPC2_peptide 4.724
IPC2.peptide.svr19 4.649
Protein with the highest isoelectric point:
>tr|E7D0U2|E7D0U2_9RHAB Phosphoprotein OS=Scophthalmus maximus rhabdovirus OX=936149 GN=P PE=4 SV=1
MM1 pKa = 6.98 EE2 pKa = 4.44 QVAKK6 pKa = 10.81 CLMDD10 pKa = 3.82 YY11 pKa = 10.33 QNRR14 pKa = 11.84 AHH16 pKa = 6.91 LRR18 pKa = 11.84 RR19 pKa = 11.84 SRR21 pKa = 11.84 MLNRR25 pKa = 11.84 YY26 pKa = 4.69 WTTYY30 pKa = 10.84 DD31 pKa = 3.41 KK32 pKa = 11.16 QSPSWGRR39 pKa = 11.84 LLEE42 pKa = 4.56 RR43 pKa = 11.84 EE44 pKa = 4.04 RR45 pKa = 11.84 QMNPIPPEE53 pKa = 4.16 VIYY56 pKa = 10.37 HH57 pKa = 5.31 QLPVDD62 pKa = 5.33 LGTCPLKK69 pKa = 10.69 RR70 pKa = 11.84 RR71 pKa = 11.84 GAIRR75 pKa = 11.84 RR76 pKa = 11.84 KK77 pKa = 8.7 RR78 pKa = 11.84 TTGSSTNPEE87 pKa = 3.99 PQSWWLRR94 pKa = 11.84 LPIIGWLLQRR104 pKa = 11.84 IRR106 pKa = 11.84 TLVLNRR112 pKa = 11.84 RR113 pKa = 11.84 TNGGRR118 pKa = 3.21
Molecular weight: 14.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.177
IPC2_protein 10.116
IPC_protein 11.389
Toseland 11.477
ProMoST 11.974
Dawson 11.491
Bjellqvist 11.462
Wikipedia 11.945
Rodwell 11.242
Grimsley 11.535
Solomon 11.959
Lehninger 11.857
Nozaki 11.477
DTASelect 11.462
Thurlkill 11.477
EMBOSS 11.974
Sillero 11.491
Patrickios 10.979
IPC_peptide 11.959
IPC2_peptide 10.935
IPC2.peptide.svr19 9.338
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3843
110
2116
549.0
62.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.557 ± 1.05
1.717 ± 0.353
5.491 ± 0.418
6.193 ± 0.548
3.435 ± 0.391
6.063 ± 0.291
2.42 ± 0.281
5.517 ± 0.564
5.673 ± 0.344
9.576 ± 0.701
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.227 ± 0.119
4.059 ± 0.266
4.97 ± 0.434
3.487 ± 0.208
6.089 ± 0.863
8.171 ± 0.722
6.167 ± 0.279
5.881 ± 0.746
2.212 ± 0.202
3.097 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here