Hermit crab associated circular virus
Average proteome isoelectric point is 8.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLN6|A0A0K1RLN6_9CIRC Putative capsid protein OS=Hermit crab associated circular virus OX=1692252 PE=4 SV=1
MM1 pKa = 7.47 PRR3 pKa = 11.84 DD4 pKa = 3.53 APAKK8 pKa = 9.06 QWCFTLNNYY17 pKa = 7.88 TPAEE21 pKa = 3.96 LTAIVDD27 pKa = 3.68 SAGNFDD33 pKa = 4.15 YY34 pKa = 11.33 LCFGRR39 pKa = 11.84 EE40 pKa = 3.57 RR41 pKa = 11.84 GNNNTPHH48 pKa = 5.96 LQGYY52 pKa = 9.48 LILKK56 pKa = 6.87 EE57 pKa = 4.27 KK58 pKa = 10.76 KK59 pKa = 7.99 RR60 pKa = 11.84 FSYY63 pKa = 10.31 VRR65 pKa = 11.84 QLAGLEE71 pKa = 3.88 RR72 pKa = 11.84 AHH74 pKa = 6.68 WEE76 pKa = 4.15 KK77 pKa = 10.75 KK78 pKa = 9.95 SPRR81 pKa = 11.84 STPKK85 pKa = 10.09 QASDD89 pKa = 3.59 YY90 pKa = 10.18 CKK92 pKa = 10.48 KK93 pKa = 10.86 DD94 pKa = 2.83 GDD96 pKa = 3.73 YY97 pKa = 11.43 DD98 pKa = 3.93 EE99 pKa = 5.87 YY100 pKa = 11.9 GEE102 pKa = 5.69 LPTKK106 pKa = 10.56 KK107 pKa = 9.78 QGQRR111 pKa = 11.84 TDD113 pKa = 3.64 FDD115 pKa = 4.24 EE116 pKa = 4.57 LKK118 pKa = 10.64 EE119 pKa = 4.19 WIKK122 pKa = 10.72 EE123 pKa = 3.84 QDD125 pKa = 3.06 HH126 pKa = 6.62 RR127 pKa = 11.84 PTDD130 pKa = 3.47 RR131 pKa = 11.84 EE132 pKa = 3.97 VAEE135 pKa = 4.78 EE136 pKa = 5.06 FPSLWGRR143 pKa = 11.84 YY144 pKa = 7.82 RR145 pKa = 11.84 SACISFLDD153 pKa = 4.41 LFSPHH158 pKa = 6.48 PTLVQGTLRR167 pKa = 11.84 PWQEE171 pKa = 4.13 DD172 pKa = 3.89 LNTRR176 pKa = 11.84 LSAPPNDD183 pKa = 3.91 RR184 pKa = 11.84 DD185 pKa = 3.54 VMFVVDD191 pKa = 4.08 EE192 pKa = 4.62 NGNSGKK198 pKa = 10.12 SWFIRR203 pKa = 11.84 YY204 pKa = 9.39 LMTEE208 pKa = 4.17 RR209 pKa = 11.84 PDD211 pKa = 3.62 DD212 pKa = 3.83 VQMLSIGKK220 pKa = 9.53 RR221 pKa = 11.84 DD222 pKa = 4.17 DD223 pKa = 3.49 LAHH226 pKa = 7.2 AIDD229 pKa = 3.96 PAKK232 pKa = 10.57 KK233 pKa = 9.81 IFFFDD238 pKa = 3.55 VPRR241 pKa = 11.84 GGMEE245 pKa = 3.96 FMQYY249 pKa = 11.06 AVLEE253 pKa = 4.12 QLKK256 pKa = 9.96 NRR258 pKa = 11.84 LVFSPKK264 pKa = 9.36 YY265 pKa = 7.58 EE266 pKa = 3.98 SRR268 pKa = 11.84 MKK270 pKa = 10.2 VLHH273 pKa = 6.83 HH274 pKa = 6.76 IPHH277 pKa = 5.61 VVVFCNEE284 pKa = 3.68 EE285 pKa = 3.72 PDD287 pKa = 3.52 RR288 pKa = 11.84 TKK290 pKa = 11.02 LSRR293 pKa = 11.84 DD294 pKa = 3.3 RR295 pKa = 11.84 FRR297 pKa = 11.84 VTHH300 pKa = 5.94 IRR302 pKa = 11.84 QVV304 pKa = 2.96
Molecular weight: 35.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.942
IPC2_protein 7.293
IPC_protein 7.307
Toseland 7.117
ProMoST 7.775
Dawson 7.966
Bjellqvist 8.17
Wikipedia 7.892
Rodwell 7.966
Grimsley 7.205
Solomon 8.083
Lehninger 8.097
Nozaki 8.39
DTASelect 8.068
Thurlkill 8.112
EMBOSS 8.214
Sillero 8.404
Patrickios 4.558
IPC_peptide 8.068
IPC2_peptide 7.322
IPC2.peptide.svr19 7.423
Protein with the highest isoelectric point:
>tr|A0A0K1RLN6|A0A0K1RLN6_9CIRC Putative capsid protein OS=Hermit crab associated circular virus OX=1692252 PE=4 SV=1
MM1 pKa = 7.53 TKK3 pKa = 10.32 FLTPPRR9 pKa = 11.84 PSSKK13 pKa = 9.97 RR14 pKa = 11.84 LRR16 pKa = 11.84 AVSPKK21 pKa = 8.94 MQTGTPEE28 pKa = 4.01 PAHH31 pKa = 6.23 TIMPRR36 pKa = 11.84 GISTHH41 pKa = 4.7 MRR43 pKa = 11.84 KK44 pKa = 9.09 SAYY47 pKa = 9.37 HH48 pKa = 6.23 RR49 pKa = 11.84 NLGRR53 pKa = 11.84 RR54 pKa = 11.84 VGKK57 pKa = 10.34 YY58 pKa = 5.52 STRR61 pKa = 11.84 RR62 pKa = 11.84 TLYY65 pKa = 10.05 TGFKK69 pKa = 10.17 NDD71 pKa = 3.7 LVSKK75 pKa = 8.46 KK76 pKa = 8.65 TYY78 pKa = 7.5 WSRR81 pKa = 11.84 LIRR84 pKa = 11.84 IPHH87 pKa = 5.69 YY88 pKa = 10.37 GAGQEE93 pKa = 3.84 EE94 pKa = 4.81 HH95 pKa = 5.74 IKK97 pKa = 10.02 YY98 pKa = 10.27 RR99 pKa = 11.84 RR100 pKa = 11.84 GAYY103 pKa = 10.2 CNVRR107 pKa = 11.84 GVKK110 pKa = 9.53 INYY113 pKa = 6.65 WFSMDD118 pKa = 5.37 ANTQYY123 pKa = 10.77 PVQVRR128 pKa = 11.84 WAIINPKK135 pKa = 9.52 VNEE138 pKa = 4.31 GTDD141 pKa = 3.57 LTLVTNPEE149 pKa = 3.73 NFFISKK155 pKa = 10.57 DD156 pKa = 3.38 PTTEE160 pKa = 3.61 QSEE163 pKa = 4.39 NFPTTGNYY171 pKa = 8.73 FKK173 pKa = 11.5 YY174 pKa = 9.78 MNRR177 pKa = 11.84 KK178 pKa = 8.68 INRR181 pKa = 11.84 EE182 pKa = 3.72 LYY184 pKa = 10.13 GVLKK188 pKa = 10.58 EE189 pKa = 4.41 GTFTMSIDD197 pKa = 3.49 PAAAVPNPGVVYY209 pKa = 10.55 RR210 pKa = 11.84 PGSAVKK216 pKa = 10.24 KK217 pKa = 8.52 IALYY221 pKa = 10.77 VPVNRR226 pKa = 11.84 QMKK229 pKa = 7.92 WNNVVAGDD237 pKa = 3.8 ANGYY241 pKa = 9.0 PEE243 pKa = 4.03 QNLYY247 pKa = 9.27 FVWWYY252 pKa = 8.42 DD253 pKa = 3.38 TAANDD258 pKa = 3.63 NTNPSASPLVQEE270 pKa = 4.48 FHH272 pKa = 7.1 EE273 pKa = 4.15 KK274 pKa = 9.9 TIYY277 pKa = 10.33 FKK279 pKa = 10.97 NSQMFAA285 pKa = 3.35
Molecular weight: 32.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.604
IPC_protein 9.75
Toseland 9.984
ProMoST 9.78
Dawson 10.248
Bjellqvist 9.955
Wikipedia 10.452
Rodwell 10.584
Grimsley 10.335
Solomon 10.262
Lehninger 10.218
Nozaki 9.97
DTASelect 9.955
Thurlkill 10.072
EMBOSS 10.409
Sillero 10.16
Patrickios 9.604
IPC_peptide 10.262
IPC2_peptide 8.624
IPC2.peptide.svr19 8.638
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
589
285
304
294.5
34.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.603 ± 0.483
1.019 ± 0.453
5.433 ± 1.78
5.603 ± 0.944
4.924 ± 0.483
5.433 ± 0.361
2.547 ± 0.299
3.905 ± 0.445
6.961 ± 0.038
6.452 ± 1.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.716 ± 0.299
5.942 ± 1.443
6.961 ± 0.276
3.735 ± 0.391
7.81 ± 0.537
5.433 ± 0.123
6.282 ± 1.213
6.282 ± 0.499
2.037 ± 0.046
4.924 ± 0.944
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here