Ignatzschineria ureiclastica
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2AEB4|A0A2U2AEB4_9GAMM Iron ABC transporter permease OS=Ignatzschineria ureiclastica OX=472582 GN=DC083_07870 PE=3 SV=1
MM1 pKa = 7.92 EE2 pKa = 5.74 DD3 pKa = 3.41 LQRR6 pKa = 11.84 IHH8 pKa = 7.03 PFEE11 pKa = 5.81 IIQYY15 pKa = 9.62 DD16 pKa = 3.89 EE17 pKa = 3.87 NHH19 pKa = 6.67 FGLFFPAHH27 pKa = 5.73 EE28 pKa = 4.13 RR29 pKa = 11.84 FEE31 pKa = 4.57 VFDD34 pKa = 4.28 ALEE37 pKa = 4.42 MEE39 pKa = 4.37 SSGYY43 pKa = 10.02 NWVDD47 pKa = 2.96 IIEE50 pKa = 4.8 FYY52 pKa = 11.24 LEE54 pKa = 4.28 NEE56 pKa = 4.13 LTDD59 pKa = 3.7 LQGAFQYY66 pKa = 10.52 EE67 pKa = 4.51 PNDD70 pKa = 3.68 EE71 pKa = 4.27 SCEE74 pKa = 4.05 LQGSFEE80 pKa = 4.9 DD81 pKa = 3.33 IKK83 pKa = 11.53 AFVLNFRR90 pKa = 11.84 PLYY93 pKa = 10.48 FDD95 pKa = 5.43 DD96 pKa = 4.58 RR97 pKa = 11.84 QLSLLIEE104 pKa = 4.4 EE105 pKa = 4.83 MQEE108 pKa = 3.07 TWYY111 pKa = 11.02
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.681
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.681
Grimsley 3.592
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.05
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.961
Patrickios 0.947
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A2U2ADF8|A0A2U2ADF8_9GAMM Ketol-acid reductoisomerase (NADP(+)) OS=Ignatzschineria ureiclastica OX=472582 GN=ilvC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.58 RR3 pKa = 11.84 TFQPSLIKK11 pKa = 10.36 RR12 pKa = 11.84 KK13 pKa = 9.5 RR14 pKa = 11.84 NHH16 pKa = 5.47 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.29 VINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.99
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1914
0
1914
660020
25
3987
344.8
38.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.582 ± 0.065
0.776 ± 0.017
5.303 ± 0.055
6.651 ± 0.066
4.269 ± 0.048
7.021 ± 0.084
2.082 ± 0.026
7.605 ± 0.056
5.416 ± 0.043
10.472 ± 0.086
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.593 ± 0.032
4.303 ± 0.067
4.037 ± 0.04
4.158 ± 0.044
4.527 ± 0.056
5.959 ± 0.04
5.5 ± 0.036
6.422 ± 0.051
1.047 ± 0.018
3.278 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here