Cyprinodon variegatus (Sheepshead minnow)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q2CCQ4|A0A3Q2CCQ4_CYPVA Family with sequence similarity 222 member A OS=Cyprinodon variegatus OX=28743 PE=4 SV=1
MM1 pKa = 7.2SVDD4 pKa = 3.86MNSQASDD11 pKa = 3.5SNEE14 pKa = 3.28EE15 pKa = 3.97DD16 pKa = 3.28FGVNSEE22 pKa = 4.55EE23 pKa = 5.1EE24 pKa = 4.05EE25 pKa = 4.5DD26 pKa = 4.11DD27 pKa = 5.39DD28 pKa = 6.34GGEE31 pKa = 4.45EE32 pKa = 5.1DD33 pKa = 4.8DD34 pKa = 4.18QGDD37 pKa = 3.28IAGYY41 pKa = 9.8YY42 pKa = 10.41DD43 pKa = 4.23GVANDD48 pKa = 4.14VEE50 pKa = 4.47QQGADD55 pKa = 3.17SFDD58 pKa = 3.61PEE60 pKa = 4.54EE61 pKa = 4.03YY62 pKa = 10.58QFTCLTYY69 pKa = 10.4KK70 pKa = 10.42EE71 pKa = 4.31SQRR74 pKa = 11.84VLIEE78 pKa = 3.9EE79 pKa = 4.51VNTVAAALKK88 pKa = 10.73SFVLSHH94 pKa = 6.04KK95 pKa = 10.0HH96 pKa = 5.47RR97 pKa = 11.84LHH99 pKa = 7.49AGVCLCGVFGGRR111 pKa = 11.84FLLMQVCQMLGCPRR125 pKa = 11.84SS126 pKa = 3.34

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q2CG25|A0A3Q2CG25_CYPVA Uncharacterized protein OS=Cyprinodon variegatus OX=28743 PE=4 SV=1
MM1 pKa = 7.49SLLGPQSSSAPRR13 pKa = 11.84LEE15 pKa = 4.71PLDD18 pKa = 3.58SSSPLRR24 pKa = 11.84GPLSSSKK31 pKa = 10.07PRR33 pKa = 11.84LGPRR37 pKa = 11.84SSSKK41 pKa = 9.95PRR43 pKa = 11.84LGARR47 pKa = 11.84SSSKK51 pKa = 10.02PRR53 pKa = 11.84SKK55 pKa = 10.5PRR57 pKa = 11.84LGPRR61 pKa = 11.84SSSKK65 pKa = 10.09PRR67 pKa = 11.84LGPRR71 pKa = 11.84SSSKK75 pKa = 10.72ARR77 pKa = 11.84LGPRR81 pKa = 11.84SSSKK85 pKa = 10.72ARR87 pKa = 11.84LGPRR91 pKa = 11.84SSSKK95 pKa = 10.72ARR97 pKa = 11.84LGPRR101 pKa = 11.84SSSPPLLGALSSSNPLRR118 pKa = 11.84GGRR121 pKa = 11.84SSSIPLRR128 pKa = 11.84GGRR131 pKa = 11.84SSSPPRR137 pKa = 11.84RR138 pKa = 11.84GPRR141 pKa = 11.84SSSKK145 pKa = 9.9PRR147 pKa = 11.84RR148 pKa = 11.84GPLSSPSLRR157 pKa = 11.84GASPLLKK164 pKa = 10.39GPSFSSLLSSRR175 pKa = 11.84PSCLQSLQRR184 pKa = 11.84KK185 pKa = 8.15EE186 pKa = 4.01GHH188 pKa = 6.56RR189 pKa = 11.84SNLDD193 pKa = 3.34RR194 pKa = 11.84NN195 pKa = 3.89

Molecular weight: 20.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22943

8938

31881

17389895

9

7455

545.5

60.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 0.012

2.233 ± 0.012

5.11 ± 0.008

6.852 ± 0.017

3.77 ± 0.01

6.268 ± 0.016

2.581 ± 0.007

4.547 ± 0.01

5.78 ± 0.015

9.579 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.006

3.973 ± 0.008

5.734 ± 0.016

4.718 ± 0.013

5.571 ± 0.01

8.83 ± 0.02

5.523 ± 0.01

6.193 ± 0.011

1.148 ± 0.004

2.774 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski