Virus sp.
Average proteome isoelectric point is 8.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345MZT1|A0A345MZT1_9VIRU Coat protein OS=Virus sp. OX=2202649 PE=4 SV=1
MM1 pKa = 7.74 PPQGFSLRR9 pKa = 11.84 KK10 pKa = 7.52 MSQRR14 pKa = 11.84 SRR16 pKa = 11.84 QKK18 pKa = 10.94 AKK20 pKa = 9.7 EE21 pKa = 3.82 KK22 pKa = 10.53 EE23 pKa = 3.85 IAKK26 pKa = 9.78 VARR29 pKa = 11.84 KK30 pKa = 9.47 VLNEE34 pKa = 3.55 KK35 pKa = 11.0 AEE37 pKa = 4.16 LKK39 pKa = 10.51 EE40 pKa = 4.6 HH41 pKa = 7.03 IFGQNTTISTTGSLFCMTEE60 pKa = 3.48 VDD62 pKa = 5.03 QGDD65 pKa = 4.21 AYY67 pKa = 11.17 NSRR70 pKa = 11.84 DD71 pKa = 3.32 GGEE74 pKa = 3.92 ISIQGIDD81 pKa = 3.53 LRR83 pKa = 11.84 YY84 pKa = 7.38 TVTYY88 pKa = 11.19 ADD90 pKa = 4.04 ATNQMRR96 pKa = 11.84 VIVFTWNDD104 pKa = 3.21 GATNPVTGDD113 pKa = 3.29 VLQAPGSWDD122 pKa = 3.15 VLSYY126 pKa = 11.14 LDD128 pKa = 3.39 RR129 pKa = 11.84 HH130 pKa = 6.31 GDD132 pKa = 3.26 VPYY135 pKa = 10.74 KK136 pKa = 10.39 VLYY139 pKa = 10.75 DD140 pKa = 3.52 KK141 pKa = 10.73 THH143 pKa = 6.46 LVDD146 pKa = 3.51 NTSNPQKK153 pKa = 10.5 NGRR156 pKa = 11.84 INLRR160 pKa = 11.84 FKK162 pKa = 11.18 NGIRR166 pKa = 11.84 CYY168 pKa = 10.67 YY169 pKa = 10.1 NGSQGTDD176 pKa = 3.2 YY177 pKa = 11.26 GKK179 pKa = 10.2 RR180 pKa = 11.84 QIWMLCISDD189 pKa = 5.13 SIASTHH195 pKa = 5.75 PAFRR199 pKa = 11.84 GVGYY203 pKa = 10.76 VYY205 pKa = 10.73 FNDD208 pKa = 3.94 FF209 pKa = 3.41
Molecular weight: 23.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.1
IPC2_protein 8.156
IPC_protein 8.156
Toseland 8.419
ProMoST 8.521
Dawson 8.843
Bjellqvist 8.887
Wikipedia 8.931
Rodwell 8.902
Grimsley 8.448
Solomon 9.019
Lehninger 8.99
Nozaki 8.931
DTASelect 8.741
Thurlkill 8.799
EMBOSS 9.004
Sillero 9.019
Patrickios 4.317
IPC_peptide 9.004
IPC2_peptide 7.717
IPC2.peptide.svr19 7.712
Protein with the highest isoelectric point:
>tr|A0A345MZT1|A0A345MZT1_9VIRU Coat protein OS=Virus sp. OX=2202649 PE=4 SV=1
MM1 pKa = 8.02 DD2 pKa = 5.4 KK3 pKa = 10.72 LVEE6 pKa = 3.88 QAIVHH11 pKa = 6.51 LANNTYY17 pKa = 10.84 SDD19 pKa = 3.58 WAWAHH24 pKa = 6.01 KK25 pKa = 10.14 EE26 pKa = 4.19 VVKK29 pKa = 11.01 SSTFRR34 pKa = 11.84 EE35 pKa = 4.11 LLQKK39 pKa = 10.03 EE40 pKa = 4.11 IKK42 pKa = 9.94 RR43 pKa = 11.84 IKK45 pKa = 10.82 NEE47 pKa = 3.88 AQQKK51 pKa = 8.13 EE52 pKa = 4.36 YY53 pKa = 10.64 HH54 pKa = 6.25 YY55 pKa = 9.55 MITFTVSPTLHH66 pKa = 5.85 PTIDD70 pKa = 3.61 NEE72 pKa = 4.44 TEE74 pKa = 3.97 KK75 pKa = 10.96 QIEE78 pKa = 4.33 DD79 pKa = 4.12 YY80 pKa = 10.77 IRR82 pKa = 11.84 SQSDD86 pKa = 3.04 RR87 pKa = 11.84 TGLHH91 pKa = 5.41 VKK93 pKa = 9.68 EE94 pKa = 3.74 MHH96 pKa = 6.19 YY97 pKa = 10.97 VKK99 pKa = 10.26 EE100 pKa = 3.84 LHH102 pKa = 6.96 KK103 pKa = 10.64 NGRR106 pKa = 11.84 PHH108 pKa = 5.52 WHH110 pKa = 6.13 VSVTTTKK117 pKa = 10.55 AIKK120 pKa = 9.88 KK121 pKa = 9.97 ALFSYY126 pKa = 8.13 YY127 pKa = 10.48 QKK129 pKa = 10.56 IYY131 pKa = 10.96 GNIDD135 pKa = 3.56 FSRR138 pKa = 11.84 SKK140 pKa = 11.09 GKK142 pKa = 10.18 NNQDD146 pKa = 2.85 ALAYY150 pKa = 8.08 MSKK153 pKa = 10.44 SGTPTKK159 pKa = 10.31 LKK161 pKa = 10.51 KK162 pKa = 10.2 VALL165 pKa = 4.11
Molecular weight: 19.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.041
IPC2_protein 9.004
IPC_protein 8.887
Toseland 9.692
ProMoST 9.37
Dawson 9.926
Bjellqvist 9.56
Wikipedia 10.072
Rodwell 10.394
Grimsley 9.999
Solomon 9.94
Lehninger 9.911
Nozaki 9.648
DTASelect 9.56
Thurlkill 9.765
EMBOSS 10.101
Sillero 9.823
Patrickios 7.614
IPC_peptide 9.94
IPC2_peptide 7.966
IPC2.peptide.svr19 8.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
374
165
209
187.0
21.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.615 ± 0.272
0.802 ± 0.49
5.882 ± 1.001
4.813 ± 1.132
3.209 ± 0.479
6.15 ± 1.904
3.476 ± 1.208
5.882 ± 0.109
9.091 ± 2.22
6.15 ± 0.316
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.406 ± 0.011
5.615 ± 0.468
2.941 ± 0.316
5.08 ± 0.141
5.08 ± 0.881
6.952 ± 0.196
7.487 ± 0.239
6.417 ± 0.218
1.604 ± 0.131
5.348 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here