Mycobacterium phage Keziacharles14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T5F3|A0A5J6T5F3_9CAUD Scaffolding protein OS=Mycobacterium phage Keziacharles14 OX=2575611 GN=14 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.92VPLDD6 pKa = 3.95DD7 pKa = 5.81LVYY10 pKa = 11.0DD11 pKa = 4.48PDD13 pKa = 4.55GPPCAPYY20 pKa = 9.73PDD22 pKa = 3.99SVLILGGYY30 pKa = 8.74RR31 pKa = 11.84WLGMNGRR38 pKa = 11.84LLTITADD45 pKa = 3.61FTEE48 pKa = 4.79WAASRR53 pKa = 11.84AAQAVEE59 pKa = 4.74DD60 pKa = 5.01DD61 pKa = 4.76EE62 pKa = 6.11DD63 pKa = 3.95PQAGVGSIYY72 pKa = 9.66GTAVNLTNRR81 pKa = 11.84TLAYY85 pKa = 9.18WEE87 pKa = 4.07AAAIRR92 pKa = 11.84GGYY95 pKa = 8.44VLSEE99 pKa = 4.17LLVV102 pKa = 3.65

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T2Q5|A0A5J6T2Q5_9CAUD Uncharacterized protein OS=Mycobacterium phage Keziacharles14 OX=2575611 GN=48 PE=4 SV=1
MM1 pKa = 7.57GRR3 pKa = 11.84VRR5 pKa = 11.84WLRR8 pKa = 11.84KK9 pKa = 9.35LFSRR13 pKa = 11.84NWDD16 pKa = 3.72RR17 pKa = 11.84YY18 pKa = 8.65PEE20 pKa = 3.94WRR22 pKa = 11.84PDD24 pKa = 3.7QILTPEE30 pKa = 4.01NRR32 pKa = 11.84SLTGQWHH39 pKa = 4.91EE40 pKa = 3.97RR41 pKa = 11.84QRR43 pKa = 11.84EE44 pKa = 3.94FEE46 pKa = 4.07RR47 pKa = 11.84ALISDD52 pKa = 4.12GWQRR56 pKa = 11.84AGTDD60 pKa = 3.8PLTGGPVYY68 pKa = 10.68VRR70 pKa = 11.84YY71 pKa = 9.71RR72 pKa = 11.84EE73 pKa = 4.11HH74 pKa = 6.76GG75 pKa = 3.42

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16631

42

968

178.8

19.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.246 ± 0.405

0.866 ± 0.123

6.271 ± 0.216

6.458 ± 0.296

3.319 ± 0.164

8.616 ± 0.419

1.864 ± 0.163

5.015 ± 0.168

4.834 ± 0.211

8.19 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.147

3.457 ± 0.151

5.045 ± 0.236

3.463 ± 0.195

5.983 ± 0.318

5.676 ± 0.241

6.428 ± 0.275

6.843 ± 0.173

1.882 ± 0.133

2.964 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski