Ostreid herpesvirus 1 (isolate France) (OsHV-1) (Pacific oyster herpesvirus)
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q6R797|Y118_OSHVF Putative RING finger protein ORF118 OS=Ostreid herpesvirus 1 (isolate France) OX=654903 GN=ORF118 PE=4 SV=1
MM1 pKa = 7.35 AAVALTYY8 pKa = 7.45 MIPPATPLAIIDD20 pKa = 4.04 FDD22 pKa = 4.19 MLPEE26 pKa = 4.99 DD27 pKa = 5.0 DD28 pKa = 4.37 EE29 pKa = 5.07 SEE31 pKa = 4.37 EE32 pKa = 4.29 VGFEE36 pKa = 4.44 YY37 pKa = 10.77 EE38 pKa = 6.37 DD39 pKa = 4.09 LLDD42 pKa = 3.82 WQLKK46 pKa = 8.26 EE47 pKa = 4.1 AVDD50 pKa = 3.96 RR51 pKa = 11.84 RR52 pKa = 11.84 DD53 pKa = 3.65 LEE55 pKa = 4.25 SFTILIEE62 pKa = 4.31 EE63 pKa = 4.36 NWCHH67 pKa = 7.38 DD68 pKa = 3.72 LTLEE72 pKa = 5.16 NGDD75 pKa = 3.76 NLLGYY80 pKa = 8.69 MIKK83 pKa = 10.0 QGHH86 pKa = 6.78 ALTEE90 pKa = 3.74 EE91 pKa = 4.44 WYY93 pKa = 10.37 DD94 pKa = 4.08 LIGVTTTCHH103 pKa = 6.3 ANPEE107 pKa = 4.24 GEE109 pKa = 4.43 LPIDD113 pKa = 4.48 LLRR116 pKa = 11.84 KK117 pKa = 9.47 HH118 pKa = 6.55 LGSCRR123 pKa = 11.84 CNLEE127 pKa = 4.43 SMNCDD132 pKa = 2.76 LVEE135 pKa = 4.04 YY136 pKa = 10.26 IIRR139 pKa = 11.84 TCYY142 pKa = 9.94 AHH144 pKa = 7.46 PINRR148 pKa = 11.84 DD149 pKa = 3.45 YY150 pKa = 11.26 VYY152 pKa = 11.11 HH153 pKa = 7.39 RR154 pKa = 11.84 MDD156 pKa = 3.44 TSRR159 pKa = 11.84 YY160 pKa = 4.41 THH162 pKa = 6.42 KK163 pKa = 10.76 RR164 pKa = 11.84 LNRR167 pKa = 11.84 NHH169 pKa = 6.65 NLTLSIEE176 pKa = 4.13 KK177 pKa = 10.48 KK178 pKa = 9.98 PIIVEE183 pKa = 4.06 APPMEE188 pKa = 4.35 EE189 pKa = 4.02 EE190 pKa = 4.55 EE191 pKa = 4.07 ISEE194 pKa = 4.57 VEE196 pKa = 3.99 DD197 pKa = 3.71 ALNVLQRR204 pKa = 11.84 LCAQEE209 pKa = 4.77 EE210 pKa = 4.61 GDD212 pKa = 3.77 NKK214 pKa = 10.5 EE215 pKa = 5.05 AEE217 pKa = 4.58 TNNNNYY223 pKa = 8.53 VFPWMTPAYY232 pKa = 7.88 EE233 pKa = 4.46 LPEE236 pKa = 3.88 QLTFYY241 pKa = 10.57 DD242 pKa = 3.65 MPMTPFNCNNVMYY255 pKa = 10.41 CC256 pKa = 4.09
Molecular weight: 29.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.077
IPC2_protein 4.317
IPC_protein 4.266
Toseland 4.113
ProMoST 4.368
Dawson 4.215
Bjellqvist 4.355
Wikipedia 4.062
Rodwell 4.101
Grimsley 4.024
Solomon 4.202
Lehninger 4.164
Nozaki 4.317
DTASelect 4.457
Thurlkill 4.113
EMBOSS 4.088
Sillero 4.38
Patrickios 0.947
IPC_peptide 4.215
IPC2_peptide 4.368
IPC2.peptide.svr19 4.267
Protein with the highest isoelectric point:
>sp|Q6R7J0|Y034_OSHVF Uncharacterized protein ORF34 OS=Ostreid herpesvirus 1 (isolate France) OX=654903 GN=ORF34 PE=4 SV=1
MM1 pKa = 7.19 STTEE5 pKa = 3.71 QTVCEE10 pKa = 4.14 IEE12 pKa = 4.23 QEE14 pKa = 4.41 SEE16 pKa = 4.53 LIPAKK21 pKa = 9.63 PQYY24 pKa = 10.65 IIVKK28 pKa = 9.46 KK29 pKa = 9.85 PKK31 pKa = 7.87 RR32 pKa = 11.84 QAWQRR37 pKa = 11.84 VLLLFRR43 pKa = 11.84 IINMIVIWAALIALFVKK60 pKa = 10.24 LYY62 pKa = 9.87 ILRR65 pKa = 11.84 GPIPRR70 pKa = 11.84 SYY72 pKa = 10.89 FHH74 pKa = 6.6 YY75 pKa = 10.81
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.181
IPC2_protein 9.253
IPC_protein 9.326
Toseland 9.721
ProMoST 9.604
Dawson 9.999
Bjellqvist 9.721
Wikipedia 10.204
Rodwell 10.277
Grimsley 10.101
Solomon 10.058
Lehninger 10.028
Nozaki 9.765
DTASelect 9.706
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.926
Patrickios 7.571
IPC_peptide 10.058
IPC2_peptide 8.492
IPC2.peptide.svr19 8.195
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
116
0
116
51428
75
1878
443.3
50.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.095 ± 0.142
1.923 ± 0.122
6.85 ± 0.135
7.539 ± 0.175
4.122 ± 0.085
4.974 ± 0.121
1.925 ± 0.08
7.123 ± 0.154
7.982 ± 0.206
7.966 ± 0.166
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.535 ± 0.123
5.658 ± 0.128
4.305 ± 0.214
2.641 ± 0.071
4.964 ± 0.146
6.234 ± 0.143
6.213 ± 0.166
6.098 ± 0.104
0.842 ± 0.047
4.011 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here