Ostreid herpesvirus 1 (isolate France) (OsHV-1) (Pacific oyster herpesvirus) 
Average proteome isoelectric point is 6.17 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 116 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>sp|Q6R797|Y118_OSHVF Putative RING finger protein ORF118 OS=Ostreid herpesvirus 1 (isolate France) OX=654903 GN=ORF118 PE=4 SV=1 
MM1 pKa = 7.35  AAVALTYY8 pKa = 7.45  MIPPATPLAIIDD20 pKa = 4.04  FDD22 pKa = 4.19  MLPEE26 pKa = 4.99  DD27 pKa = 5.0  DD28 pKa = 4.37  EE29 pKa = 5.07  SEE31 pKa = 4.37  EE32 pKa = 4.29  VGFEE36 pKa = 4.44  YY37 pKa = 10.77  EE38 pKa = 6.37  DD39 pKa = 4.09  LLDD42 pKa = 3.82  WQLKK46 pKa = 8.26  EE47 pKa = 4.1  AVDD50 pKa = 3.96  RR51 pKa = 11.84  RR52 pKa = 11.84  DD53 pKa = 3.65  LEE55 pKa = 4.25  SFTILIEE62 pKa = 4.31  EE63 pKa = 4.36  NWCHH67 pKa = 7.38  DD68 pKa = 3.72  LTLEE72 pKa = 5.16  NGDD75 pKa = 3.76  NLLGYY80 pKa = 8.69  MIKK83 pKa = 10.0  QGHH86 pKa = 6.78  ALTEE90 pKa = 3.74  EE91 pKa = 4.44  WYY93 pKa = 10.37  DD94 pKa = 4.08  LIGVTTTCHH103 pKa = 6.3  ANPEE107 pKa = 4.24  GEE109 pKa = 4.43  LPIDD113 pKa = 4.48  LLRR116 pKa = 11.84  KK117 pKa = 9.47  HH118 pKa = 6.55  LGSCRR123 pKa = 11.84  CNLEE127 pKa = 4.43  SMNCDD132 pKa = 2.76  LVEE135 pKa = 4.04  YY136 pKa = 10.26  IIRR139 pKa = 11.84  TCYY142 pKa = 9.94  AHH144 pKa = 7.46  PINRR148 pKa = 11.84  DD149 pKa = 3.45  YY150 pKa = 11.26  VYY152 pKa = 11.11  HH153 pKa = 7.39  RR154 pKa = 11.84  MDD156 pKa = 3.44  TSRR159 pKa = 11.84  YY160 pKa = 4.41  THH162 pKa = 6.42  KK163 pKa = 10.76  RR164 pKa = 11.84  LNRR167 pKa = 11.84  NHH169 pKa = 6.65  NLTLSIEE176 pKa = 4.13  KK177 pKa = 10.48  KK178 pKa = 9.98  PIIVEE183 pKa = 4.06  APPMEE188 pKa = 4.35  EE189 pKa = 4.02  EE190 pKa = 4.55  EE191 pKa = 4.07  ISEE194 pKa = 4.57  VEE196 pKa = 3.99  DD197 pKa = 3.71  ALNVLQRR204 pKa = 11.84  LCAQEE209 pKa = 4.77  EE210 pKa = 4.61  GDD212 pKa = 3.77  NKK214 pKa = 10.5  EE215 pKa = 5.05  AEE217 pKa = 4.58  TNNNNYY223 pKa = 8.53  VFPWMTPAYY232 pKa = 7.88  EE233 pKa = 4.46  LPEE236 pKa = 3.88  QLTFYY241 pKa = 10.57  DD242 pKa = 3.65  MPMTPFNCNNVMYY255 pKa = 10.41  CC256 pKa = 4.09   
 Molecular weight: 29.83 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.077 
IPC2_protein 4.317 
IPC_protein 4.266 
Toseland    4.113 
ProMoST     4.368 
Dawson      4.215 
Bjellqvist  4.355 
Wikipedia   4.062 
Rodwell     4.101 
Grimsley    4.024 
Solomon     4.202 
Lehninger   4.164 
Nozaki      4.317 
DTASelect   4.457 
Thurlkill   4.113 
EMBOSS      4.088 
Sillero     4.38 
Patrickios  0.947 
IPC_peptide 4.215 
IPC2_peptide  4.368 
IPC2.peptide.svr19  4.267 
 Protein with the highest isoelectric point: 
>sp|Q6R7J0|Y034_OSHVF Uncharacterized protein ORF34 OS=Ostreid herpesvirus 1 (isolate France) OX=654903 GN=ORF34 PE=4 SV=1 
MM1 pKa = 7.19  STTEE5 pKa = 3.71  QTVCEE10 pKa = 4.14  IEE12 pKa = 4.23  QEE14 pKa = 4.41  SEE16 pKa = 4.53  LIPAKK21 pKa = 9.63  PQYY24 pKa = 10.65  IIVKK28 pKa = 9.46  KK29 pKa = 9.85  PKK31 pKa = 7.87  RR32 pKa = 11.84  QAWQRR37 pKa = 11.84  VLLLFRR43 pKa = 11.84  IINMIVIWAALIALFVKK60 pKa = 10.24  LYY62 pKa = 9.87  ILRR65 pKa = 11.84  GPIPRR70 pKa = 11.84  SYY72 pKa = 10.89  FHH74 pKa = 6.6  YY75 pKa = 10.81   
 Molecular weight: 8.92 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.181 
IPC2_protein 9.253 
IPC_protein 9.326 
Toseland    9.721 
ProMoST     9.604 
Dawson      9.999 
Bjellqvist  9.721 
Wikipedia   10.204 
Rodwell     10.277 
Grimsley    10.101 
Solomon     10.058 
Lehninger   10.028 
Nozaki      9.765 
DTASelect   9.706 
Thurlkill   9.823 
EMBOSS      10.145 
Sillero     9.926 
Patrickios  7.571 
IPC_peptide 10.058 
IPC2_peptide  8.492 
IPC2.peptide.svr19  8.195 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        116 
 
        
        0
 
        
        116 
         
        51428
 
        75
 
        1878
 
        443.3
 
        50.72
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        5.095 ± 0.142
1.923 ± 0.122
 
        6.85 ± 0.135
7.539 ± 0.175
 
        4.122 ± 0.085
4.974 ± 0.121
 
        1.925 ± 0.08
7.123 ± 0.154
       
        7.982 ± 0.206
7.966 ± 0.166
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        3.535 ± 0.123
5.658 ± 0.128
 
        4.305 ± 0.214
2.641 ± 0.071
 
        4.964 ± 0.146
6.234 ± 0.143
 
        6.213 ± 0.166
6.098 ± 0.104
       
        0.842 ± 0.047
4.011 ± 0.1
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here