Amino acid dipepetide frequency for Firmicutes bacterium CAG:110

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.358AlaAla: 11.358 ± 0.203	1.543AlaCys: 1.543 ± 0.047	5.46AlaAsp: 5.46 ± 0.094	6.334AlaGlu: 6.334 ± 0.097	3.513AlaPhe: 3.513 ± 0.072	7.24AlaGly: 7.24 ± 0.115	1.467AlaHis: 1.467 ± 0.051	5.259AlaIle: 5.259 ± 0.1	4.602AlaLys: 4.602 ± 0.083	9.213AlaLeu: 9.213 ± 0.137	3.138AlaMet: 3.138 ± 0.075	2.572AlaAsn: 2.572 ± 0.058	3.301AlaPro: 3.301 ± 0.087	3.149AlaGln: 3.149 ± 0.078	4.107AlaArg: 4.107 ± 0.084	4.384AlaSer: 4.384 ± 0.072	3.867AlaThr: 3.867 ± 0.088	7.983AlaVal: 7.983 ± 0.13	0.874AlaTrp: 0.874 ± 0.034	3.04AlaTyr: 3.04 ± 0.063	0.01AlaXaa: 0.01 ± 0.004
Cys	1.624CysAla: 1.624 ± 0.046	0.376CysCys: 0.376 ± 0.03	1.03CysAsp: 1.03 ± 0.042	0.885CysGlu: 0.885 ± 0.043	0.74CysPhe: 0.74 ± 0.036	1.932CysGly: 1.932 ± 0.067	0.358CysHis: 0.358 ± 0.025	1.036CysIle: 1.036 ± 0.041	0.734CysLys: 0.734 ± 0.03	1.476CysLeu: 1.476 ± 0.052	0.476CysMet: 0.476 ± 0.026	0.582CysAsn: 0.582 ± 0.029	0.784CysPro: 0.784 ± 0.037	0.501CysGln: 0.501 ± 0.029	1.182CysArg: 1.182 ± 0.043	0.986CysSer: 0.986 ± 0.041	0.983CysThr: 0.983 ± 0.046	1.212CysVal: 1.212 ± 0.04	0.153CysTrp: 0.153 ± 0.014	0.557CysTyr: 0.557 ± 0.028	0.001CysXaa: 0.001 ± 0.002
Asp	5.486AspAla: 5.486 ± 0.09	1.03AspCys: 1.03 ± 0.042	3.014AspAsp: 3.014 ± 0.073	3.847AspGlu: 3.847 ± 0.081	2.581AspPhe: 2.581 ± 0.065	5.372AspGly: 5.372 ± 0.11	0.953AspHis: 0.953 ± 0.042	3.658AspIle: 3.658 ± 0.08	2.572AspLys: 2.572 ± 0.062	4.584AspLeu: 4.584 ± 0.092	1.757AspMet: 1.757 ± 0.055	1.855AspAsn: 1.855 ± 0.049	2.351AspPro: 2.351 ± 0.051	1.318AspGln: 1.318 ± 0.042	2.709AspArg: 2.709 ± 0.067	3.134AspSer: 3.134 ± 0.07	3.448AspThr: 3.448 ± 0.08	4.153AspVal: 4.153 ± 0.091	0.688AspTrp: 0.688 ± 0.032	2.658AspTyr: 2.658 ± 0.064	0.0AspXaa: 0.0 ± 0.0
Glu	5.825GluAla: 5.825 ± 0.109	0.781GluCys: 0.781 ± 0.036	3.715GluAsp: 3.715 ± 0.081	4.922GluGlu: 4.922 ± 0.09	2.146GluPhe: 2.146 ± 0.056	4.125GluGly: 4.125 ± 0.083	1.168GluHis: 1.168 ± 0.041	4.139GluIle: 4.139 ± 0.095	4.727GluLys: 4.727 ± 0.083	6.181GluLeu: 6.181 ± 0.089	2.017GluMet: 2.017 ± 0.062	3.423GluAsn: 3.423 ± 0.065	2.161GluPro: 2.161 ± 0.064	2.591GluGln: 2.591 ± 0.063	3.165GluArg: 3.165 ± 0.08	2.955GluSer: 2.955 ± 0.056	4.019GluThr: 4.019 ± 0.083	3.861GluVal: 3.861 ± 0.096	0.595GluTrp: 0.595 ± 0.034	2.192GluTyr: 2.192 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	3.641PheAla: 3.641 ± 0.073	0.831PheCys: 0.831 ± 0.035	2.608PheAsp: 2.608 ± 0.068	1.984PheGlu: 1.984 ± 0.062	1.747PhePhe: 1.747 ± 0.056	3.26PheGly: 3.26 ± 0.074	0.828PheHis: 0.828 ± 0.04	1.993PheIle: 1.993 ± 0.054	1.234PheLys: 1.234 ± 0.048	3.885PheLeu: 3.885 ± 0.085	0.858PheMet: 0.858 ± 0.032	1.256PheAsn: 1.256 ± 0.044	1.622PhePro: 1.622 ± 0.052	1.259PheGln: 1.259 ± 0.047	2.153PheArg: 2.153 ± 0.054	2.784PheSer: 2.784 ± 0.07	2.523PheThr: 2.523 ± 0.065	2.686PheVal: 2.686 ± 0.063	0.452PheTrp: 0.452 ± 0.028	1.5PheTyr: 1.5 ± 0.044	0.001PheXaa: 0.001 ± 0.001
Gly	6.379GlyAla: 6.379 ± 0.132	1.572GlyCys: 1.572 ± 0.056	4.138GlyAsp: 4.138 ± 0.078	4.993GlyGlu: 4.993 ± 0.096	3.165GlyPhe: 3.165 ± 0.08	6.297GlyGly: 6.297 ± 0.149	1.43GlyHis: 1.43 ± 0.055	5.369GlyIle: 5.369 ± 0.092	5.215GlyLys: 5.215 ± 0.092	6.821GlyLeu: 6.821 ± 0.093	2.553GlyMet: 2.553 ± 0.069	2.936GlyAsn: 2.936 ± 0.065	1.372GlyPro: 1.372 ± 0.05	2.472GlyGln: 2.472 ± 0.067	3.69GlyArg: 3.69 ± 0.081	4.631GlySer: 4.631 ± 0.082	4.872GlyThr: 4.872 ± 0.109	5.895GlyVal: 5.895 ± 0.099	1.033GlyTrp: 1.033 ± 0.053	3.31GlyTyr: 3.31 ± 0.08	0.004GlyXaa: 0.004 ± 0.002
His	1.256HisAla: 1.256 ± 0.041	0.359HisCys: 0.359 ± 0.023	1.024HisAsp: 1.024 ± 0.046	0.918HisGlu: 0.918 ± 0.038	0.908HisPhe: 0.908 ± 0.032	1.536HisGly: 1.536 ± 0.053	0.487HisHis: 0.487 ± 0.042	1.242HisIle: 1.242 ± 0.041	0.763HisLys: 0.763 ± 0.031	1.551HisLeu: 1.551 ± 0.053	0.473HisMet: 0.473 ± 0.027	0.639HisAsn: 0.639 ± 0.032	1.043HisPro: 1.043 ± 0.04	0.547HisGln: 0.547 ± 0.029	1.003HisArg: 1.003 ± 0.047	0.912HisSer: 0.912 ± 0.039	1.098HisThr: 1.098 ± 0.05	1.146HisVal: 1.146 ± 0.038	0.251HisTrp: 0.251 ± 0.017	0.76HisTyr: 0.76 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.703IleAla: 5.703 ± 0.094	1.215IleCys: 1.215 ± 0.051	3.311IleAsp: 3.311 ± 0.07	2.957IleGlu: 2.957 ± 0.077	2.256IlePhe: 2.256 ± 0.052	4.473IleGly: 4.473 ± 0.085	1.097IleHis: 1.097 ± 0.04	3.249IleIle: 3.249 ± 0.076	2.141IleLys: 2.141 ± 0.057	6.259IleLeu: 6.259 ± 0.108	1.426IleMet: 1.426 ± 0.044	1.881IleAsn: 1.881 ± 0.061	3.257IlePro: 3.257 ± 0.075	1.875IleGln: 1.875 ± 0.057	3.652IleArg: 3.652 ± 0.083	3.845IleSer: 3.845 ± 0.094	3.688IleThr: 3.688 ± 0.082	4.401IleVal: 4.401 ± 0.088	0.629IleTrp: 0.629 ± 0.031	2.091IleTyr: 2.091 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.725LysAla: 4.725 ± 0.087	0.699LysCys: 0.699 ± 0.034	2.751LysAsp: 2.751 ± 0.076	3.679LysGlu: 3.679 ± 0.076	1.472LysPhe: 1.472 ± 0.051	3.351LysGly: 3.351 ± 0.069	0.784LysHis: 0.784 ± 0.035	3.108LysIle: 3.108 ± 0.071	3.665LysLys: 3.665 ± 0.066	4.81LysLeu: 4.81 ± 0.086	1.591LysMet: 1.591 ± 0.05	2.264LysAsn: 2.264 ± 0.063	2.146LysPro: 2.146 ± 0.053	1.857LysGln: 1.857 ± 0.057	2.676LysArg: 2.676 ± 0.063	2.536LysSer: 2.536 ± 0.059	3.256LysThr: 3.256 ± 0.078	3.428LysVal: 3.428 ± 0.08	0.521LysTrp: 0.521 ± 0.029	1.974LysTyr: 1.974 ± 0.055	0.001LysXaa: 0.001 ± 0.001
Leu	8.408LeuAla: 8.408 ± 0.121	1.908LeuCys: 1.908 ± 0.055	5.483LeuAsp: 5.483 ± 0.079	5.776LeuGlu: 5.776 ± 0.106	3.635LeuPhe: 3.635 ± 0.086	6.911LeuGly: 6.911 ± 0.106	1.771LeuHis: 1.771 ± 0.055	5.044LeuIle: 5.044 ± 0.103	4.56LeuLys: 4.56 ± 0.081	9.969LeuLeu: 9.969 ± 0.155	2.652LeuMet: 2.652 ± 0.075	3.612LeuAsn: 3.612 ± 0.057	4.592LeuPro: 4.592 ± 0.094	2.852LeuGln: 2.852 ± 0.076	5.452LeuArg: 5.452 ± 0.118	6.379LeuSer: 6.379 ± 0.115	6.169LeuThr: 6.169 ± 0.097	6.128LeuVal: 6.128 ± 0.106	1.02LeuTrp: 1.02 ± 0.039	3.256LeuTyr: 3.256 ± 0.077	0.0LeuXaa: 0.0 ± 0.0
Met	2.872MetAla: 2.872 ± 0.067	0.365MetCys: 0.365 ± 0.022	1.901MetAsp: 1.901 ± 0.054	2.278MetGlu: 2.278 ± 0.052	0.864MetPhe: 0.864 ± 0.033	2.217MetGly: 2.217 ± 0.054	0.391MetHis: 0.391 ± 0.021	1.44MetIle: 1.44 ± 0.052	1.993MetLys: 1.993 ± 0.053	2.837MetLeu: 2.837 ± 0.066	0.776MetMet: 0.776 ± 0.037	1.233MetAsn: 1.233 ± 0.039	1.24MetPro: 1.24 ± 0.045	0.939MetGln: 0.939 ± 0.033	1.398MetArg: 1.398 ± 0.047	1.58MetSer: 1.58 ± 0.05	1.859MetThr: 1.859 ± 0.055	2.159MetVal: 2.159 ± 0.055	0.222MetTrp: 0.222 ± 0.016	0.803MetTyr: 0.803 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.462AsnAla: 3.462 ± 0.074	0.648AsnCys: 0.648 ± 0.03	1.786AsnAsp: 1.786 ± 0.049	1.847AsnGlu: 1.847 ± 0.058	1.361AsnPhe: 1.361 ± 0.047	3.267AsnGly: 3.267 ± 0.076	0.713AsnHis: 0.713 ± 0.034	2.284AsnIle: 2.284 ± 0.062	1.467AsnLys: 1.467 ± 0.051	3.477AsnLeu: 3.477 ± 0.063	1.028AsnMet: 1.028 ± 0.04	1.202AsnAsn: 1.202 ± 0.045	2.095AsnPro: 2.095 ± 0.055	1.105AsnGln: 1.105 ± 0.039	1.997AsnArg: 1.997 ± 0.055	1.763AsnSer: 1.763 ± 0.048	2.061AsnThr: 2.061 ± 0.063	2.75AsnVal: 2.75 ± 0.06	0.473AsnTrp: 0.473 ± 0.031	1.465AsnTyr: 1.465 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	3.786ProAla: 3.786 ± 0.085	0.597ProCys: 0.597 ± 0.03	2.776ProAsp: 2.776 ± 0.063	4.003ProGlu: 4.003 ± 0.089	1.541ProPhe: 1.541 ± 0.048	3.344ProGly: 3.344 ± 0.083	0.651ProHis: 0.651 ± 0.028	2.119ProIle: 2.119 ± 0.055	2.001ProLys: 2.001 ± 0.062	3.173ProLeu: 3.173 ± 0.064	1.172ProMet: 1.172 ± 0.043	1.359ProAsn: 1.359 ± 0.044	1.224ProPro: 1.224 ± 0.061	1.43ProGln: 1.43 ± 0.052	1.56ProArg: 1.56 ± 0.047	1.884ProSer: 1.884 ± 0.056	2.207ProThr: 2.207 ± 0.072	3.514ProVal: 3.514 ± 0.08	0.462ProTrp: 0.462 ± 0.028	1.504ProTyr: 1.504 ± 0.05	0.001ProXaa: 0.001 ± 0.001
Gln	2.986GlnAla: 2.986 ± 0.063	0.44GlnCys: 0.44 ± 0.026	1.511GlnAsp: 1.511 ± 0.045	2.229GlnGlu: 2.229 ± 0.066	1.146GlnPhe: 1.146 ± 0.038	2.023GlnGly: 2.023 ± 0.051	0.503GlnHis: 0.503 ± 0.024	2.09GlnIle: 2.09 ± 0.056	2.006GlnLys: 2.006 ± 0.059	3.099GlnLeu: 3.099 ± 0.07	1.186GlnMet: 1.186 ± 0.04	1.354GlnAsn: 1.354 ± 0.045	1.308GlnPro: 1.308 ± 0.043	1.227GlnGln: 1.227 ± 0.053	1.892GlnArg: 1.892 ± 0.06	1.864GlnSer: 1.864 ± 0.051	1.899GlnThr: 1.899 ± 0.055	2.212GlnVal: 2.212 ± 0.061	0.388GlnTrp: 0.388 ± 0.026	1.222GlnTyr: 1.222 ± 0.044	0.001GlnXaa: 0.001 ± 0.001
Arg	3.805ArgAla: 3.805 ± 0.085	0.942ArgCys: 0.942 ± 0.036	2.822ArgAsp: 2.822 ± 0.064	4.162ArgGlu: 4.162 ± 0.096	2.224ArgPhe: 2.224 ± 0.059	3.281ArgGly: 3.281 ± 0.081	0.98ArgHis: 0.98 ± 0.037	3.264ArgIle: 3.264 ± 0.071	3.198ArgLys: 3.198 ± 0.074	5.22ArgLeu: 5.22 ± 0.094	1.646ArgMet: 1.646 ± 0.05	2.023ArgAsn: 2.023 ± 0.053	1.855ArgPro: 1.855 ± 0.056	2.151ArgGln: 2.151 ± 0.066	3.524ArgArg: 3.524 ± 0.088	2.661ArgSer: 2.661 ± 0.059	2.57ArgThr: 2.57 ± 0.065	3.335ArgVal: 3.335 ± 0.08	0.536ArgTrp: 0.536 ± 0.027	2.087ArgTyr: 2.087 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	5.163SerAla: 5.163 ± 0.091	0.955SerCys: 0.955 ± 0.045	3.223SerAsp: 3.223 ± 0.065	3.101SerGlu: 3.101 ± 0.073	2.494SerPhe: 2.494 ± 0.054	5.432SerGly: 5.432 ± 0.089	1.041SerHis: 1.041 ± 0.04	3.199SerIle: 3.199 ± 0.079	2.354SerLys: 2.354 ± 0.064	5.161SerLeu: 5.161 ± 0.094	1.624SerMet: 1.624 ± 0.045	1.635SerAsn: 1.635 ± 0.05	2.119SerPro: 2.119 ± 0.055	1.791SerGln: 1.791 ± 0.055	3.186SerArg: 3.186 ± 0.07	3.06SerSer: 3.06 ± 0.088	2.921SerThr: 2.921 ± 0.067	4.459SerVal: 4.459 ± 0.089	0.642SerTrp: 0.642 ± 0.031	2.148SerTyr: 2.148 ± 0.06	0.003SerXaa: 0.003 ± 0.002
Thr	5.912ThrAla: 5.912 ± 0.11	0.855ThrCys: 0.855 ± 0.044	3.459ThrAsp: 3.459 ± 0.089	3.574ThrGlu: 3.574 ± 0.098	2.227ThrPhe: 2.227 ± 0.077	5.047ThrGly: 5.047 ± 0.104	1.04ThrHis: 1.04 ± 0.045	3.588ThrIle: 3.588 ± 0.08	2.389ThrLys: 2.389 ± 0.06	5.673ThrLeu: 5.673 ± 0.098	1.56ThrMet: 1.56 ± 0.046	1.705ThrAsn: 1.705 ± 0.053	2.888ThrPro: 2.888 ± 0.064	1.723ThrGln: 1.723 ± 0.054	2.45ThrArg: 2.45 ± 0.056	2.761ThrSer: 2.761 ± 0.065	3.135ThrThr: 3.135 ± 0.095	5.794ThrVal: 5.794 ± 0.131	0.551ThrTrp: 0.551 ± 0.032	2.05ThrTyr: 2.05 ± 0.066	0.0ThrXaa: 0.0 ± 0.0
Val	5.984ValAla: 5.984 ± 0.095	1.557ValCys: 1.557 ± 0.053	4.058ValAsp: 4.058 ± 0.066	4.34ValGlu: 4.34 ± 0.09	2.984ValPhe: 2.984 ± 0.077	5.125ValGly: 5.125 ± 0.087	1.25ValHis: 1.25 ± 0.045	4.645ValIle: 4.645 ± 0.076	3.612ValLys: 3.612 ± 0.074	7.509ValLeu: 7.509 ± 0.116	2.259ValMet: 2.259 ± 0.061	2.727ValAsn: 2.727 ± 0.067	3.099ValPro: 3.099 ± 0.073	2.074ValGln: 2.074 ± 0.063	3.71ValArg: 3.71 ± 0.075	4.936ValSer: 4.936 ± 0.102	4.972ValThr: 4.972 ± 0.11	5.264ValVal: 5.264 ± 0.111	0.778ValTrp: 0.778 ± 0.032	2.641ValTyr: 2.641 ± 0.063	0.0ValXaa: 0.0 ± 0.0
Trp	0.84TrpAla: 0.84 ± 0.037	0.203TrpCys: 0.203 ± 0.018	0.703TrpAsp: 0.703 ± 0.038	0.726TrpGlu: 0.726 ± 0.033	0.446TrpPhe: 0.446 ± 0.029	0.831TrpGly: 0.831 ± 0.038	0.216TrpHis: 0.216 ± 0.018	0.618TrpIle: 0.618 ± 0.027	0.645TrpLys: 0.645 ± 0.03	1.124TrpLeu: 1.124 ± 0.041	0.321TrpMet: 0.321 ± 0.022	0.533TrpAsn: 0.533 ± 0.029	0.28TrpPro: 0.28 ± 0.02	0.506TrpGln: 0.506 ± 0.028	0.51TrpArg: 0.51 ± 0.027	0.582TrpSer: 0.582 ± 0.03	0.509TrpThr: 0.509 ± 0.032	0.69TrpVal: 0.69 ± 0.032	0.156TrpTrp: 0.156 ± 0.015	0.443TrpTyr: 0.443 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.195TyrAla: 3.195 ± 0.066	0.733TyrCys: 0.733 ± 0.035	2.392TyrAsp: 2.392 ± 0.065	2.153TyrGlu: 2.153 ± 0.052	1.655TyrPhe: 1.655 ± 0.052	3.078TyrGly: 3.078 ± 0.068	0.786TyrHis: 0.786 ± 0.031	2.061TyrIle: 2.061 ± 0.053	1.415TyrLys: 1.415 ± 0.058	3.554TyrLeu: 3.554 ± 0.081	0.851TyrMet: 0.851 ± 0.032	1.459TyrAsn: 1.459 ± 0.045	1.567TyrPro: 1.567 ± 0.046	1.219TyrGln: 1.219 ± 0.045	2.266TyrArg: 2.266 ± 0.058	2.075TyrSer: 2.075 ± 0.055	2.328TyrThr: 2.328 ± 0.082	2.455TyrVal: 2.455 ± 0.06	0.457TyrTrp: 0.457 ± 0.03	1.611TyrTyr: 1.611 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.006XaaGly: 0.006 ± 0.003	0.001XaaHis: 0.001 ± 0.002	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.004XaaArg: 0.004 ± 0.003	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.002	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.03XaaXaa: 0.03 ± 0.009

Statistics based on 2311 proteins (703987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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