Rhodoferax phage P26218
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3GMB1|A0A0E3GMB1_9CAUD Portal protein OS=Rhodoferax phage P26218 OX=1636270 GN=P26218_39 PE=4 SV=1
MM1 pKa = 7.93 PGPNPSILNMRR12 pKa = 11.84 SGQNAIAGGGTQFGWISGDD31 pKa = 3.11 IAGLALSATAIAIFDD46 pKa = 5.02 LGPNWDD52 pKa = 3.73 QYY54 pKa = 11.86 NVVQVGVVPAGPSSGLSAVQVFSSDD79 pKa = 3.25 DD80 pKa = 3.35 AAFNATTDD88 pKa = 3.54 VQLNNTWATTFGAISAAITTPQTSGFSPMGRR119 pKa = 11.84 YY120 pKa = 10.08 LIVRR124 pKa = 11.84 ATNADD129 pKa = 3.9 GINAQGAGAFIRR141 pKa = 11.84 VTAYY145 pKa = 10.01 PFVV148 pKa = 3.56
Molecular weight: 15.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.274
IPC2_protein 4.151
IPC_protein 4.012
Toseland 3.783
ProMoST 4.253
Dawson 4.062
Bjellqvist 4.215
Wikipedia 4.088
Rodwell 3.846
Grimsley 3.694
Solomon 4.037
Lehninger 3.986
Nozaki 4.202
DTASelect 4.533
Thurlkill 3.897
EMBOSS 4.088
Sillero 4.151
Patrickios 3.719
IPC_peptide 4.012
IPC2_peptide 4.113
IPC2.peptide.svr19 4.072
Protein with the highest isoelectric point:
>tr|A0A0E3M2S5|A0A0E3M2S5_9CAUD Uncharacterized protein OS=Rhodoferax phage P26218 OX=1636270 GN=P26218_18 PE=4 SV=1
MM1 pKa = 6.82 TTTTKK6 pKa = 10.6 LVPTFVSSNIGAAAEE21 pKa = 4.01 ALFNANRR28 pKa = 11.84 ARR30 pKa = 11.84 PCACYY35 pKa = 8.81 TQDD38 pKa = 3.31 GQAVVCSARR47 pKa = 11.84 TARR50 pKa = 11.84 KK51 pKa = 9.83 NGWTIVARR59 pKa = 11.84 TWKK62 pKa = 10.55
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.281
IPC2_protein 9.297
IPC_protein 9.516
Toseland 10.233
ProMoST 10.043
Dawson 10.365
Bjellqvist 10.072
Wikipedia 10.511
Rodwell 10.745
Grimsley 10.409
Solomon 10.452
Lehninger 10.438
Nozaki 10.35
DTASelect 10.014
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.321
Patrickios 10.657
IPC_peptide 10.452
IPC2_peptide 9.443
IPC2.peptide.svr19 8.164
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
11236
60
730
255.4
27.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.579 ± 0.708
0.881 ± 0.122
5.66 ± 0.342
5.411 ± 0.393
2.91 ± 0.191
8.143 ± 0.384
1.744 ± 0.248
4.486 ± 0.253
5.571 ± 0.388
7.912 ± 0.434
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.106 ± 0.183
3.818 ± 0.32
5.028 ± 0.194
4.735 ± 0.41
5.278 ± 0.324
5.028 ± 0.24
6.942 ± 0.564
7.316 ± 0.278
1.397 ± 0.124
3.053 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here