Rhodoferax phage P26218

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0E3GMB1|A0A0E3GMB1_9CAUD Portal protein OS=Rhodoferax phage P26218 OX=1636270 GN=P26218_39 PE=4 SV=1
MM1 pKa = 7.93PGPNPSILNMRR12 pKa = 11.84SGQNAIAGGGTQFGWISGDD31 pKa = 3.11IAGLALSATAIAIFDD46 pKa = 5.02LGPNWDD52 pKa = 3.73QYY54 pKa = 11.86NVVQVGVVPAGPSSGLSAVQVFSSDD79 pKa = 3.25DD80 pKa = 3.35AAFNATTDD88 pKa = 3.54VQLNNTWATTFGAISAAITTPQTSGFSPMGRR119 pKa = 11.84YY120 pKa = 10.08LIVRR124 pKa = 11.84ATNADD129 pKa = 3.9GINAQGAGAFIRR141 pKa = 11.84VTAYY145 pKa = 10.01PFVV148 pKa = 3.56

Molecular weight:
15.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0E3M2S5|A0A0E3M2S5_9CAUD Uncharacterized protein OS=Rhodoferax phage P26218 OX=1636270 GN=P26218_18 PE=4 SV=1
MM1 pKa = 6.82TTTTKK6 pKa = 10.6LVPTFVSSNIGAAAEE21 pKa = 4.01ALFNANRR28 pKa = 11.84ARR30 pKa = 11.84PCACYY35 pKa = 8.81TQDD38 pKa = 3.31GQAVVCSARR47 pKa = 11.84TARR50 pKa = 11.84KK51 pKa = 9.83NGWTIVARR59 pKa = 11.84TWKK62 pKa = 10.55

Molecular weight:
6.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

11236

60

730

255.4

27.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.579 ± 0.708

0.881 ± 0.122

5.66 ± 0.342

5.411 ± 0.393

2.91 ± 0.191

8.143 ± 0.384

1.744 ± 0.248

4.486 ± 0.253

5.571 ± 0.388

7.912 ± 0.434

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.106 ± 0.183

3.818 ± 0.32

5.028 ± 0.194

4.735 ± 0.41

5.278 ± 0.324

5.028 ± 0.24

6.942 ± 0.564

7.316 ± 0.278

1.397 ± 0.124

3.053 ± 0.255

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski