Duck faeces associated circular DNA virus 1
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161J5M7|A0A161J5M7_9VIRU Putative capsid protein OS=Duck faeces associated circular DNA virus 1 OX=1843768 PE=4 SV=1
MM1 pKa = 8.02 PSFQINSKK9 pKa = 10.33 SLFLTFPQCQTSLLRR24 pKa = 11.84 FQEE27 pKa = 4.47 LINGFFGKK35 pKa = 10.02 NLEE38 pKa = 4.48 KK39 pKa = 10.87 GVICQEE45 pKa = 3.89 NHH47 pKa = 7.03 KK48 pKa = 10.65 DD49 pKa = 3.71 SQDD52 pKa = 3.48 LHH54 pKa = 7.72 LHH56 pKa = 6.09 AAICLKK62 pKa = 9.41 EE63 pKa = 4.23 TLRR66 pKa = 11.84 SRR68 pKa = 11.84 DD69 pKa = 3.49 PRR71 pKa = 11.84 VFDD74 pKa = 4.51 CLVEE78 pKa = 4.1 PAQHH82 pKa = 6.59 GDD84 pKa = 2.58 IEE86 pKa = 4.6 TRR88 pKa = 11.84 FKK90 pKa = 11.4 GGVLKK95 pKa = 10.89 AFQYY99 pKa = 10.89 VMKK102 pKa = 10.35 DD103 pKa = 3.28 GNYY106 pKa = 10.3 LPLGQGFDD114 pKa = 3.97 LNLFMEE120 pKa = 4.4 QATKK124 pKa = 10.54 KK125 pKa = 10.12 KK126 pKa = 8.81 CTKK129 pKa = 9.73 TSLILKK135 pKa = 8.96 DD136 pKa = 3.43 IQAGATLDD144 pKa = 3.77 SLVPEE149 pKa = 4.13 HH150 pKa = 7.52 AEE152 pKa = 3.77 YY153 pKa = 11.17 LLQNLQKK160 pKa = 10.76 VKK162 pKa = 10.75 AYY164 pKa = 10.97 LDD166 pKa = 3.73 FLSLRR171 pKa = 11.84 EE172 pKa = 3.91 KK173 pKa = 10.58 RR174 pKa = 11.84 LAFAEE179 pKa = 4.43 GQTQKK184 pKa = 10.05 VHH186 pKa = 5.41 VMPAEE191 pKa = 5.4 GYY193 pKa = 9.07 CLSWNQEE200 pKa = 3.36 IASWLNLNLRR210 pKa = 11.84 QKK212 pKa = 10.36 RR213 pKa = 11.84 SHH215 pKa = 6.06 RR216 pKa = 11.84 QKK218 pKa = 11.2 QMWICAPPGMGKK230 pKa = 8.33 TSMIMMLEE238 pKa = 3.96 KK239 pKa = 10.39 AYY241 pKa = 10.45 DD242 pKa = 3.6 LQVYY246 pKa = 7.16 YY247 pKa = 9.92 WPKK250 pKa = 10.78 EE251 pKa = 4.09 EE252 pKa = 4.32 VWWDD256 pKa = 3.44 AYY258 pKa = 11.62 SDD260 pKa = 3.81 GAYY263 pKa = 10.64 DD264 pKa = 6.07 LIVLDD269 pKa = 4.8 EE270 pKa = 4.25 FHH272 pKa = 6.47 SQKK275 pKa = 9.92 TITQLNPILSGDD287 pKa = 3.7 PTPLSRR293 pKa = 11.84 RR294 pKa = 11.84 CSAPLTKK301 pKa = 9.44 RR302 pKa = 11.84 DD303 pKa = 3.95 NLPVIIMSNYY313 pKa = 9.7 LPEE316 pKa = 4.14 EE317 pKa = 4.45 CFSKK321 pKa = 10.87 VAANAPSKK329 pKa = 10.28 LAPLIDD335 pKa = 3.69 RR336 pKa = 11.84 LTIVNVQGPIRR347 pKa = 11.84 MAPVLEE353 pKa = 5.25 DD354 pKa = 4.05 DD355 pKa = 4.14 PQEE358 pKa = 4.05 VLAALTDD365 pKa = 3.58 EE366 pKa = 4.33 TSYY369 pKa = 11.58 VQLTPSPGTPNLRR382 pKa = 11.84 QFGDD386 pKa = 4.24 DD387 pKa = 3.64 EE388 pKa = 4.73 EE389 pKa = 5.42 DD390 pKa = 3.06 WFAEE394 pKa = 4.16 EE395 pKa = 4.06 EE396 pKa = 4.05 FRR398 pKa = 11.84 RR399 pKa = 11.84 NNDD402 pKa = 2.7 FNLYY406 pKa = 10.1 NKK408 pKa = 9.88 VSII411 pKa = 4.48
Molecular weight: 47.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.48
IPC2_protein 5.525
IPC_protein 5.512
Toseland 5.715
ProMoST 5.779
Dawson 5.664
Bjellqvist 5.715
Wikipedia 5.614
Rodwell 5.626
Grimsley 5.779
Solomon 5.664
Lehninger 5.639
Nozaki 5.881
DTASelect 6.046
Thurlkill 5.944
EMBOSS 5.906
Sillero 5.97
Patrickios 4.113
IPC_peptide 5.677
IPC2_peptide 5.957
IPC2.peptide.svr19 6.0
Protein with the highest isoelectric point:
>tr|A0A161J5M7|A0A161J5M7_9VIRU Putative capsid protein OS=Duck faeces associated circular DNA virus 1 OX=1843768 PE=4 SV=1
MM1 pKa = 7.36 LGYY4 pKa = 10.6 RR5 pKa = 11.84 LRR7 pKa = 11.84 EE8 pKa = 4.03 KK9 pKa = 10.06 QAKK12 pKa = 6.55 EE13 pKa = 3.61 ARR15 pKa = 11.84 GKK17 pKa = 9.82 GVRR20 pKa = 11.84 RR21 pKa = 11.84 TGALVMRR28 pKa = 11.84 QRR30 pKa = 11.84 SRR32 pKa = 11.84 LAASSVARR40 pKa = 11.84 MPAYY44 pKa = 9.54 IRR46 pKa = 11.84 PTTNIYY52 pKa = 9.66 QRR54 pKa = 11.84 SGDD57 pKa = 3.94 KK58 pKa = 10.7 KK59 pKa = 11.43 GMDD62 pKa = 3.5 TDD64 pKa = 3.44 ILGVSTIASATASSAGVGVLNLIQTGSGSWNRR96 pKa = 11.84 VGRR99 pKa = 11.84 KK100 pKa = 8.89 IFPKK104 pKa = 9.9 SVRR107 pKa = 11.84 LRR109 pKa = 11.84 YY110 pKa = 10.25 GIDD113 pKa = 3.19 YY114 pKa = 8.41 TYY116 pKa = 11.49 SNDD119 pKa = 3.55 GDD121 pKa = 3.64 QTYY124 pKa = 9.47 PLSVRR129 pKa = 11.84 VLLVWDD135 pKa = 4.85 ANPSGGAKK143 pKa = 10.13 PLFTEE148 pKa = 4.19 MFKK151 pKa = 10.51 DD152 pKa = 3.48 TDD154 pKa = 3.43 QTGAEE159 pKa = 4.25 TTSWRR164 pKa = 11.84 SGPAYY169 pKa = 10.31 DD170 pKa = 3.67 AMGRR174 pKa = 11.84 FTILRR179 pKa = 11.84 DD180 pKa = 3.48 KK181 pKa = 11.13 VHH183 pKa = 6.17 TFVPEE188 pKa = 3.99 VFPLPVTNGLARR200 pKa = 11.84 SRR202 pKa = 11.84 IDD204 pKa = 3.05 CDD206 pKa = 3.19 EE207 pKa = 4.2 FVPLQQAMEE216 pKa = 4.33 TVYY219 pKa = 10.69 SGQNNPMTIADD230 pKa = 4.12 IYY232 pKa = 11.21 SGALYY237 pKa = 9.94 IVSICDD243 pKa = 3.24 SSPPIGYY250 pKa = 9.75 GIQFSPYY257 pKa = 7.37 SWARR261 pKa = 11.84 LRR263 pKa = 11.84 YY264 pKa = 7.82 TDD266 pKa = 3.41
Molecular weight: 29.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.855
IPC2_protein 9.224
IPC_protein 9.385
Toseland 9.502
ProMoST 9.443
Dawson 9.853
Bjellqvist 9.633
Wikipedia 10.101
Rodwell 9.955
Grimsley 9.97
Solomon 9.897
Lehninger 9.838
Nozaki 9.56
DTASelect 9.619
Thurlkill 9.663
EMBOSS 9.955
Sillero 9.78
Patrickios 4.673
IPC_peptide 9.882
IPC2_peptide 8.463
IPC2.peptide.svr19 8.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
677
266
411
338.5
38.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.942 ± 0.536
1.625 ± 0.492
6.056 ± 0.023
5.022 ± 1.346
3.988 ± 0.552
6.352 ± 1.504
1.329 ± 0.537
5.022 ± 0.347
5.465 ± 0.961
10.044 ± 1.846
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.806 ± 0.113
4.136 ± 0.635
5.761 ± 0.068
5.318 ± 0.877
6.056 ± 1.459
7.09 ± 1.088
5.761 ± 0.99
5.613 ± 0.65
1.625 ± 0.068
3.988 ± 0.718
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here