Prochlorococcus phage P-GSP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Lingvirus; Prochlorococcus virus PGSP1

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1UH44|M1UH44_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-GSP1 OX=382262 GN=PRQG_00049 PE=4 SV=1
MM1 pKa = 7.85AEE3 pKa = 4.04TLSYY7 pKa = 11.53DD8 pKa = 3.5NTPDD12 pKa = 3.65TEE14 pKa = 4.28VLSAEE19 pKa = 4.27EE20 pKa = 4.01QNSLEE25 pKa = 4.18VGEE28 pKa = 4.69KK29 pKa = 10.23LVAEE33 pKa = 4.33QEE35 pKa = 3.93QLLAGKK41 pKa = 9.68YY42 pKa = 9.68KK43 pKa = 10.55SAEE46 pKa = 4.1EE47 pKa = 4.34LEE49 pKa = 4.11NAYY52 pKa = 10.84LSLQKK57 pKa = 10.91KK58 pKa = 9.98LGQTEE63 pKa = 4.23EE64 pKa = 4.5EE65 pKa = 4.21EE66 pKa = 4.07VDD68 pKa = 3.9YY69 pKa = 11.36EE70 pKa = 4.46STDD73 pKa = 3.01EE74 pKa = 4.64GYY76 pKa = 10.77EE77 pKa = 3.96EE78 pKa = 5.14EE79 pKa = 4.42EE80 pKa = 4.71GSDD83 pKa = 3.85EE84 pKa = 4.29EE85 pKa = 4.47VSEE88 pKa = 4.08YY89 pKa = 11.28APAINLINDD98 pKa = 3.26ASEE101 pKa = 4.03EE102 pKa = 4.54YY103 pKa = 10.12YY104 pKa = 11.43ANDD107 pKa = 3.73GQLSEE112 pKa = 4.49DD113 pKa = 4.05TISAFSSMSSQDD125 pKa = 3.35LVNAYY130 pKa = 10.69LEE132 pKa = 4.25IQQNNPQASQGVEE145 pKa = 3.64LSEE148 pKa = 4.3SQVNQVQNAAGGEE161 pKa = 4.01ANYY164 pKa = 10.58NQVINWAANNLEE176 pKa = 4.49DD177 pKa = 4.74AAIDD181 pKa = 3.85AFDD184 pKa = 3.91SVVDD188 pKa = 4.1SGNPMAIQIAFQGLQAEE205 pKa = 4.5YY206 pKa = 10.94NEE208 pKa = 4.22ANGYY212 pKa = 7.67EE213 pKa = 3.83GRR215 pKa = 11.84MLQGRR220 pKa = 11.84AASSAGEE227 pKa = 4.24TFRR230 pKa = 11.84SQAEE234 pKa = 4.31LVSAMGDD241 pKa = 3.33PRR243 pKa = 11.84YY244 pKa = 9.31DD245 pKa = 2.91TDD247 pKa = 2.99EE248 pKa = 4.73AYY250 pKa = 10.59RR251 pKa = 11.84DD252 pKa = 3.73DD253 pKa = 4.22VLRR256 pKa = 11.84KK257 pKa = 9.64LDD259 pKa = 4.1ASDD262 pKa = 3.73LQFF265 pKa = 5.03

Molecular weight: 29.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1UAN0|M1UAN0_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-GSP1 OX=382262 GN=PRQG_00033 PE=4 SV=1
MM1 pKa = 7.87AKK3 pKa = 10.07RR4 pKa = 11.84GLYY7 pKa = 10.71ANIHH11 pKa = 5.93AKK13 pKa = 9.92RR14 pKa = 11.84LRR16 pKa = 11.84IKK18 pKa = 10.33KK19 pKa = 10.36GSGEE23 pKa = 4.07KK24 pKa = 9.08MRR26 pKa = 11.84KK27 pKa = 8.68PGQAGAPTAANFKK40 pKa = 10.12RR41 pKa = 11.84AAKK44 pKa = 7.72TAKK47 pKa = 10.08RR48 pKa = 11.84PRR50 pKa = 3.52

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11676

32

1213

238.3

26.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.181 ± 0.556

0.728 ± 0.171

6.098 ± 0.34

5.713 ± 0.535

3.537 ± 0.242

7.083 ± 0.384

1.584 ± 0.154

5.704 ± 0.287

6.509 ± 0.696

7.742 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.283

5.935 ± 0.36

3.828 ± 0.312

4.351 ± 0.281

4.479 ± 0.381

7.014 ± 0.5

7.588 ± 0.64

5.987 ± 0.291

1.139 ± 0.112

3.503 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski