Erysiphe necator mitovirus 2
Average proteome isoelectric point is 8.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I5ARE9|A0A2I5ARE9_9VIRU RNA-dependent RNA polymerase OS=Erysiphe necator mitovirus 2 OX=2052562 PE=4 SV=1
MM1 pKa = 7.47 LWTGRR6 pKa = 11.84 SYY8 pKa = 10.9 FPSIPEE14 pKa = 3.78 EE15 pKa = 3.86 LLQEE19 pKa = 4.31 SVRR22 pKa = 11.84 KK23 pKa = 10.35 LEE25 pKa = 4.04 TDD27 pKa = 4.15 FEE29 pKa = 5.24 NNLSRR34 pKa = 11.84 GRR36 pKa = 11.84 VAVSDD41 pKa = 3.39 KK42 pKa = 10.63 FKK44 pKa = 10.85 AYY46 pKa = 10.14 RR47 pKa = 11.84 LSLTRR52 pKa = 11.84 YY53 pKa = 9.21 VSGSPIRR60 pKa = 11.84 LEE62 pKa = 5.45 GIVQDD67 pKa = 4.22 RR68 pKa = 11.84 NLLPTVLPVDD78 pKa = 4.16 LRR80 pKa = 11.84 RR81 pKa = 11.84 LIVNRR86 pKa = 11.84 DD87 pKa = 3.09 LNAIRR92 pKa = 11.84 WALTLFTISRR102 pKa = 11.84 TVMGGKK108 pKa = 8.74 PVNYY112 pKa = 9.77 DD113 pKa = 3.6 DD114 pKa = 3.87 IVRR117 pKa = 11.84 PGPHH121 pKa = 7.63 PDD123 pKa = 3.48 QIHH126 pKa = 5.84 TEE128 pKa = 3.64 ISDD131 pKa = 3.69 YY132 pKa = 11.55 DD133 pKa = 3.95 VVQFWQRR140 pKa = 11.84 LGRR143 pKa = 11.84 PKK145 pKa = 10.09 IDD147 pKa = 3.3 WKK149 pKa = 10.14 WKK151 pKa = 8.12 NFHH154 pKa = 6.8 WSTKK158 pKa = 9.94 SGPNGPALQGALADD172 pKa = 4.69 LKK174 pKa = 11.09 CLTPDD179 pKa = 5.07 LIQAIDD185 pKa = 3.14 SFMPVKK191 pKa = 10.91 NPLTTLLEE199 pKa = 4.57 LKK201 pKa = 10.45 DD202 pKa = 4.25 SPLVAHH208 pKa = 7.85 WYY210 pKa = 9.76 QVFKK214 pKa = 11.08 VKK216 pKa = 10.62 SRR218 pKa = 11.84 GKK220 pKa = 9.99 LRR222 pKa = 11.84 KK223 pKa = 9.61 LSIKK227 pKa = 10.14 DD228 pKa = 3.18 DD229 pKa = 3.98 RR230 pKa = 11.84 EE231 pKa = 3.95 AKK233 pKa = 10.32 SRR235 pKa = 11.84 VFAILDD241 pKa = 3.56 YY242 pKa = 10.39 WSQTALQEE250 pKa = 4.01 LHH252 pKa = 6.78 KK253 pKa = 10.96 GAFKK257 pKa = 10.92 LLRR260 pKa = 11.84 RR261 pKa = 11.84 LPRR264 pKa = 11.84 DD265 pKa = 3.37 CTYY268 pKa = 11.43 DD269 pKa = 3.28 QGKK272 pKa = 9.23 LLSEE276 pKa = 4.56 LANKK280 pKa = 8.24 KK281 pKa = 8.33 TDD283 pKa = 3.11 HH284 pKa = 6.33 QYY286 pKa = 11.46 YY287 pKa = 10.67 SFDD290 pKa = 3.82 LSSATDD296 pKa = 4.02 RR297 pKa = 11.84 FPLDD301 pKa = 2.99 IQEE304 pKa = 4.26 RR305 pKa = 11.84 FVRR308 pKa = 11.84 LLTNKK313 pKa = 9.91 DD314 pKa = 3.37 VAKK317 pKa = 9.69 SWRR320 pKa = 11.84 KK321 pKa = 8.79 ILVGEE326 pKa = 4.62 EE327 pKa = 3.86 YY328 pKa = 10.62 VAPNGLSYY336 pKa = 10.78 KK337 pKa = 9.72 YY338 pKa = 10.92 GCGQPMGAHH347 pKa = 6.01 SSWPIFTLCHH357 pKa = 5.65 HH358 pKa = 6.14 MVVFVAARR366 pKa = 11.84 NCGIKK371 pKa = 10.18 RR372 pKa = 11.84 FYY374 pKa = 10.95 DD375 pKa = 3.71 YY376 pKa = 11.19 AILGDD381 pKa = 4.99 DD382 pKa = 3.61 IVISHH387 pKa = 6.87 DD388 pKa = 3.55 QVAAEE393 pKa = 4.13 YY394 pKa = 10.57 KK395 pKa = 10.4 RR396 pKa = 11.84 IISALGVEE404 pKa = 4.3 LSEE407 pKa = 4.62 AKK409 pKa = 10.36 SHH411 pKa = 5.26 VSKK414 pKa = 10.34 DD415 pKa = 3.07 TFEE418 pKa = 4.04 FAKK421 pKa = 10.22 RR422 pKa = 11.84 WFHH425 pKa = 6.55 KK426 pKa = 10.44 SVEE429 pKa = 3.98 ISPFPLHH436 pKa = 6.61 GLISTLKK443 pKa = 9.79 SWPQLMEE450 pKa = 4.19 LLINEE455 pKa = 4.3 VPKK458 pKa = 10.57 RR459 pKa = 11.84 GYY461 pKa = 10.51 RR462 pKa = 11.84 SILDD466 pKa = 3.61 FSPGLNSFVDD476 pKa = 4.34 LFPSIRR482 pKa = 11.84 MGKK485 pKa = 8.12 TILKK489 pKa = 9.98 RR490 pKa = 11.84 IEE492 pKa = 4.4 LNLHH496 pKa = 6.56 LPYY499 pKa = 9.76 MWTSEE504 pKa = 3.95 EE505 pKa = 4.13 GDD507 pKa = 3.63 VNEE510 pKa = 4.31 RR511 pKa = 11.84 CQPLFTYY518 pKa = 10.0 APSMSHH524 pKa = 6.16 WPNDD528 pKa = 3.51 QLFSTLTKK536 pKa = 8.86 CASMIARR543 pKa = 11.84 RR544 pKa = 11.84 DD545 pKa = 3.4 LGKK548 pKa = 10.56 AISDD552 pKa = 3.45 VSRR555 pKa = 11.84 TLDD558 pKa = 3.82 RR559 pKa = 11.84 LWSDD563 pKa = 3.52 LTSSLEE569 pKa = 4.03 SSAVDD574 pKa = 3.27 QSASLAPSFVDD585 pKa = 4.09 NIPMIKK591 pKa = 9.9 VLKK594 pKa = 10.33 DD595 pKa = 3.31 LSDD598 pKa = 4.76 PDD600 pKa = 4.07 TQFLGSVPLGKK611 pKa = 8.69 LTQGWDD617 pKa = 2.84 VWKK620 pKa = 10.15 AWRR623 pKa = 11.84 NIDD626 pKa = 4.47 LLNIPKK632 pKa = 10.49 LNGILPMRR640 pKa = 11.84 ALEE643 pKa = 3.98 RR644 pKa = 11.84 KK645 pKa = 8.78 VNSRR649 pKa = 11.84 AHH651 pKa = 5.63 LSLRR655 pKa = 11.84 LMQYY659 pKa = 7.43 VTKK662 pKa = 10.69 LPYY665 pKa = 9.5 QQIIEE670 pKa = 4.37 EE671 pKa = 4.29 LTAYY675 pKa = 8.41 DD676 pKa = 3.58 APRR679 pKa = 11.84 VRR681 pKa = 11.84 KK682 pKa = 9.48 RR683 pKa = 11.84 RR684 pKa = 11.84 PRR686 pKa = 11.84 SS687 pKa = 3.21
Molecular weight: 78.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.843
IPC2_protein 8.799
IPC_protein 8.697
Toseland 9.443
ProMoST 9.209
Dawson 9.721
Bjellqvist 9.443
Wikipedia 9.882
Rodwell 9.999
Grimsley 9.794
Solomon 9.75
Lehninger 9.706
Nozaki 9.545
DTASelect 9.399
Thurlkill 9.56
EMBOSS 9.882
Sillero 9.663
Patrickios 5.13
IPC_peptide 9.736
IPC2_peptide 8.024
IPC2.peptide.svr19 7.815
Protein with the highest isoelectric point:
>tr|A0A2I5ARE9|A0A2I5ARE9_9VIRU RNA-dependent RNA polymerase OS=Erysiphe necator mitovirus 2 OX=2052562 PE=4 SV=1
MM1 pKa = 7.47 LWTGRR6 pKa = 11.84 SYY8 pKa = 10.9 FPSIPEE14 pKa = 3.78 EE15 pKa = 3.86 LLQEE19 pKa = 4.31 SVRR22 pKa = 11.84 KK23 pKa = 10.35 LEE25 pKa = 4.04 TDD27 pKa = 4.15 FEE29 pKa = 5.24 NNLSRR34 pKa = 11.84 GRR36 pKa = 11.84 VAVSDD41 pKa = 3.39 KK42 pKa = 10.63 FKK44 pKa = 10.85 AYY46 pKa = 10.14 RR47 pKa = 11.84 LSLTRR52 pKa = 11.84 YY53 pKa = 9.21 VSGSPIRR60 pKa = 11.84 LEE62 pKa = 5.45 GIVQDD67 pKa = 4.22 RR68 pKa = 11.84 NLLPTVLPVDD78 pKa = 4.16 LRR80 pKa = 11.84 RR81 pKa = 11.84 LIVNRR86 pKa = 11.84 DD87 pKa = 3.09 LNAIRR92 pKa = 11.84 WALTLFTISRR102 pKa = 11.84 TVMGGKK108 pKa = 8.74 PVNYY112 pKa = 9.77 DD113 pKa = 3.6 DD114 pKa = 3.87 IVRR117 pKa = 11.84 PGPHH121 pKa = 7.63 PDD123 pKa = 3.48 QIHH126 pKa = 5.84 TEE128 pKa = 3.64 ISDD131 pKa = 3.69 YY132 pKa = 11.55 DD133 pKa = 3.95 VVQFWQRR140 pKa = 11.84 LGRR143 pKa = 11.84 PKK145 pKa = 10.09 IDD147 pKa = 3.3 WKK149 pKa = 10.14 WKK151 pKa = 8.12 NFHH154 pKa = 6.8 WSTKK158 pKa = 9.94 SGPNGPALQGALADD172 pKa = 4.69 LKK174 pKa = 11.09 CLTPDD179 pKa = 5.07 LIQAIDD185 pKa = 3.14 SFMPVKK191 pKa = 10.91 NPLTTLLEE199 pKa = 4.57 LKK201 pKa = 10.45 DD202 pKa = 4.25 SPLVAHH208 pKa = 7.85 WYY210 pKa = 9.76 QVFKK214 pKa = 11.08 VKK216 pKa = 10.62 SRR218 pKa = 11.84 GKK220 pKa = 9.99 LRR222 pKa = 11.84 KK223 pKa = 9.61 LSIKK227 pKa = 10.14 DD228 pKa = 3.18 DD229 pKa = 3.98 RR230 pKa = 11.84 EE231 pKa = 3.95 AKK233 pKa = 10.32 SRR235 pKa = 11.84 VFAILDD241 pKa = 3.56 YY242 pKa = 10.39 WSQTALQEE250 pKa = 4.01 LHH252 pKa = 6.78 KK253 pKa = 10.96 GAFKK257 pKa = 10.92 LLRR260 pKa = 11.84 RR261 pKa = 11.84 LPRR264 pKa = 11.84 DD265 pKa = 3.37 CTYY268 pKa = 11.43 DD269 pKa = 3.28 QGKK272 pKa = 9.23 LLSEE276 pKa = 4.56 LANKK280 pKa = 8.24 KK281 pKa = 8.33 TDD283 pKa = 3.11 HH284 pKa = 6.33 QYY286 pKa = 11.46 YY287 pKa = 10.67 SFDD290 pKa = 3.82 LSSATDD296 pKa = 4.02 RR297 pKa = 11.84 FPLDD301 pKa = 2.99 IQEE304 pKa = 4.26 RR305 pKa = 11.84 FVRR308 pKa = 11.84 LLTNKK313 pKa = 9.91 DD314 pKa = 3.37 VAKK317 pKa = 9.69 SWRR320 pKa = 11.84 KK321 pKa = 8.79 ILVGEE326 pKa = 4.62 EE327 pKa = 3.86 YY328 pKa = 10.62 VAPNGLSYY336 pKa = 10.78 KK337 pKa = 9.72 YY338 pKa = 10.92 GCGQPMGAHH347 pKa = 6.01 SSWPIFTLCHH357 pKa = 5.65 HH358 pKa = 6.14 MVVFVAARR366 pKa = 11.84 NCGIKK371 pKa = 10.18 RR372 pKa = 11.84 FYY374 pKa = 10.95 DD375 pKa = 3.71 YY376 pKa = 11.19 AILGDD381 pKa = 4.99 DD382 pKa = 3.61 IVISHH387 pKa = 6.87 DD388 pKa = 3.55 QVAAEE393 pKa = 4.13 YY394 pKa = 10.57 KK395 pKa = 10.4 RR396 pKa = 11.84 IISALGVEE404 pKa = 4.3 LSEE407 pKa = 4.62 AKK409 pKa = 10.36 SHH411 pKa = 5.26 VSKK414 pKa = 10.34 DD415 pKa = 3.07 TFEE418 pKa = 4.04 FAKK421 pKa = 10.22 RR422 pKa = 11.84 WFHH425 pKa = 6.55 KK426 pKa = 10.44 SVEE429 pKa = 3.98 ISPFPLHH436 pKa = 6.61 GLISTLKK443 pKa = 9.79 SWPQLMEE450 pKa = 4.19 LLINEE455 pKa = 4.3 VPKK458 pKa = 10.57 RR459 pKa = 11.84 GYY461 pKa = 10.51 RR462 pKa = 11.84 SILDD466 pKa = 3.61 FSPGLNSFVDD476 pKa = 4.34 LFPSIRR482 pKa = 11.84 MGKK485 pKa = 8.12 TILKK489 pKa = 9.98 RR490 pKa = 11.84 IEE492 pKa = 4.4 LNLHH496 pKa = 6.56 LPYY499 pKa = 9.76 MWTSEE504 pKa = 3.95 EE505 pKa = 4.13 GDD507 pKa = 3.63 VNEE510 pKa = 4.31 RR511 pKa = 11.84 CQPLFTYY518 pKa = 10.0 APSMSHH524 pKa = 6.16 WPNDD528 pKa = 3.51 QLFSTLTKK536 pKa = 8.86 CASMIARR543 pKa = 11.84 RR544 pKa = 11.84 DD545 pKa = 3.4 LGKK548 pKa = 10.56 AISDD552 pKa = 3.45 VSRR555 pKa = 11.84 TLDD558 pKa = 3.82 RR559 pKa = 11.84 LWSDD563 pKa = 3.52 LTSSLEE569 pKa = 4.03 SSAVDD574 pKa = 3.27 QSASLAPSFVDD585 pKa = 4.09 NIPMIKK591 pKa = 9.9 VLKK594 pKa = 10.33 DD595 pKa = 3.31 LSDD598 pKa = 4.76 PDD600 pKa = 4.07 TQFLGSVPLGKK611 pKa = 8.69 LTQGWDD617 pKa = 2.84 VWKK620 pKa = 10.15 AWRR623 pKa = 11.84 NIDD626 pKa = 4.47 LLNIPKK632 pKa = 10.49 LNGILPMRR640 pKa = 11.84 ALEE643 pKa = 3.98 RR644 pKa = 11.84 KK645 pKa = 8.78 VNSRR649 pKa = 11.84 AHH651 pKa = 5.63 LSLRR655 pKa = 11.84 LMQYY659 pKa = 7.43 VTKK662 pKa = 10.69 LPYY665 pKa = 9.5 QQIIEE670 pKa = 4.37 EE671 pKa = 4.29 LTAYY675 pKa = 8.41 DD676 pKa = 3.58 APRR679 pKa = 11.84 VRR681 pKa = 11.84 KK682 pKa = 9.48 RR683 pKa = 11.84 RR684 pKa = 11.84 PRR686 pKa = 11.84 SS687 pKa = 3.21
Molecular weight: 78.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.843
IPC2_protein 8.799
IPC_protein 8.697
Toseland 9.443
ProMoST 9.209
Dawson 9.721
Bjellqvist 9.443
Wikipedia 9.882
Rodwell 9.999
Grimsley 9.794
Solomon 9.75
Lehninger 9.706
Nozaki 9.545
DTASelect 9.399
Thurlkill 9.56
EMBOSS 9.882
Sillero 9.663
Patrickios 5.13
IPC_peptide 9.736
IPC2_peptide 8.024
IPC2.peptide.svr19 7.815
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
687
687
687
687.0
78.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.531 ± 0.0
1.019 ± 0.0
6.696 ± 0.0
4.221 ± 0.0
3.93 ± 0.0
4.658 ± 0.0
2.329 ± 0.0
5.677 ± 0.0
6.696 ± 0.0
12.373 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.892 ± 0.0
3.348 ± 0.0
5.822 ± 0.0
3.493 ± 0.0
7.278 ± 0.0
8.588 ± 0.0
4.512 ± 0.0
6.114 ± 0.0
2.62 ± 0.0
3.202 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here