Medicago sativa alphapartitivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus; unclassified Alphapartitivirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385JBF6|A0A385JBF6_9VIRU Coat protein OS=Medicago sativa alphapartitivirus 1 OX=2043550 PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 4.74KK3 pKa = 10.76SKK5 pKa = 11.01KK6 pKa = 9.36YY7 pKa = 10.85LSDD10 pKa = 3.02AKK12 pKa = 10.71EE13 pKa = 3.89IIKK16 pKa = 10.98ANGLEE21 pKa = 4.21EE22 pKa = 4.52KK23 pKa = 10.42FLAAAGLSSMDD34 pKa = 3.78EE35 pKa = 4.06LDD37 pKa = 3.8PKK39 pKa = 11.09NFAEE43 pKa = 4.98FEE45 pKa = 4.3APAPPQVMKK54 pKa = 10.28PSIAAAPIIQKK65 pKa = 9.84PLSKK69 pKa = 10.31QVADD73 pKa = 3.97ASTGFNPEE81 pKa = 3.99SASDD85 pKa = 3.5MLRR88 pKa = 11.84PFIGLKK94 pKa = 10.03INYY97 pKa = 7.69KK98 pKa = 9.93ARR100 pKa = 11.84KK101 pKa = 6.69TVSTYY106 pKa = 11.11APSSLMMDD114 pKa = 4.4YY115 pKa = 10.57ILHH118 pKa = 5.37QVNNLLVDD126 pKa = 3.41NFYY129 pKa = 10.97FRR131 pKa = 11.84RR132 pKa = 11.84ACPDD136 pKa = 2.97YY137 pKa = 10.77HH138 pKa = 7.74PYY140 pKa = 10.25ILRR143 pKa = 11.84LYY145 pKa = 9.92YY146 pKa = 10.59GVLFWIQCMRR156 pKa = 11.84AMNHH160 pKa = 5.49ARR162 pKa = 11.84LLHH165 pKa = 6.71AEE167 pKa = 4.49ASQCLSTFLDD177 pKa = 4.02AFPADD182 pKa = 4.08SLPISSPLLDD192 pKa = 4.12LFKK195 pKa = 10.22TLCCSQPEE203 pKa = 4.19IPTFGVVSPTLPFHH217 pKa = 7.02PGPEE221 pKa = 3.86ARR223 pKa = 11.84SAFMLADD230 pKa = 3.78ATSHH234 pKa = 5.53VLPNIPGIFALIEE247 pKa = 3.92NLRR250 pKa = 11.84TCLNPGDD257 pKa = 4.12GQQPNLPAKK266 pKa = 9.72GKK268 pKa = 9.37HH269 pKa = 5.58IPVTSTAAAATVFGHH284 pKa = 5.93HH285 pKa = 5.92QFPILANRR293 pKa = 11.84SNAEE297 pKa = 3.73KK298 pKa = 10.15WSLCSSGLQYY308 pKa = 10.61PCEE311 pKa = 4.14ADD313 pKa = 3.62RR314 pKa = 11.84RR315 pKa = 11.84LHH317 pKa = 6.25EE318 pKa = 4.57NFSEE322 pKa = 4.19RR323 pKa = 11.84LDD325 pKa = 3.54NFEE328 pKa = 4.55FPVTAEE334 pKa = 4.25DD335 pKa = 3.99DD336 pKa = 3.89DD337 pKa = 4.33LRR339 pKa = 11.84FYY341 pKa = 11.56SDD343 pKa = 4.22FLHH346 pKa = 6.27ITNSLAWFAQVRR358 pKa = 11.84DD359 pKa = 3.82VAANEE364 pKa = 3.76AAFFNGSGTLADD376 pKa = 4.56CPPFGIAANQITAGYY391 pKa = 8.08LAPIIQVAAPTRR403 pKa = 11.84SADD406 pKa = 3.65PASLFPFAIKK416 pKa = 10.79LEE418 pKa = 4.33TASRR422 pKa = 11.84ALPEE426 pKa = 4.14LAEE429 pKa = 4.29AMAALAQTNVNIYY442 pKa = 7.25PTHH445 pKa = 7.22PYY447 pKa = 9.2YY448 pKa = 11.32NHH450 pKa = 6.86IGAEE454 pKa = 4.2SRR456 pKa = 11.84RR457 pKa = 11.84GPFWNLAPVEE467 pKa = 4.19SSITDD472 pKa = 3.27HH473 pKa = 7.49SSYY476 pKa = 11.09LSLRR480 pKa = 11.84EE481 pKa = 3.94VVRR484 pKa = 11.84GLMKK488 pKa = 10.7PKK490 pKa = 10.6GG491 pKa = 3.55

Molecular weight: 53.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385JBF6|A0A385JBF6_9VIRU Coat protein OS=Medicago sativa alphapartitivirus 1 OX=2043550 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.08NIEE5 pKa = 4.28IIGYY9 pKa = 9.0KK10 pKa = 9.96PSLAKK15 pKa = 10.17PIRR18 pKa = 11.84GNVDD22 pKa = 3.08PNSNINYY29 pKa = 9.75GNIVDD34 pKa = 3.94YY35 pKa = 11.06ALRR38 pKa = 11.84KK39 pKa = 9.71YY40 pKa = 8.78LTNEE44 pKa = 3.32EE45 pKa = 4.11FTIVTRR51 pKa = 11.84GYY53 pKa = 10.52RR54 pKa = 11.84RR55 pKa = 11.84SQWAEE60 pKa = 3.74DD61 pKa = 3.74SLKK64 pKa = 10.91SDD66 pKa = 4.49LDD68 pKa = 3.99KK69 pKa = 11.43LDD71 pKa = 3.68SDD73 pKa = 4.4YY74 pKa = 11.36FPVLKK79 pKa = 9.55DD80 pKa = 2.8THH82 pKa = 6.76YY83 pKa = 11.29YY84 pKa = 9.71NAIEE88 pKa = 4.18HH89 pKa = 5.37TRR91 pKa = 11.84KK92 pKa = 9.8LFKK95 pKa = 10.03PDD97 pKa = 3.52SLLKK101 pKa = 10.13PIHH104 pKa = 6.27FSDD107 pKa = 3.45LRR109 pKa = 11.84HH110 pKa = 5.83YY111 pKa = 9.77PWQLSTNIGAPFATSKK127 pKa = 10.27SWNEE131 pKa = 3.5YY132 pKa = 9.73VIQKK136 pKa = 9.9FDD138 pKa = 3.33EE139 pKa = 5.01GFTKK143 pKa = 10.05SYY145 pKa = 10.77YY146 pKa = 9.76RR147 pKa = 11.84DD148 pKa = 3.6LFRR151 pKa = 11.84EE152 pKa = 4.02AHH154 pKa = 6.16GEE156 pKa = 4.04SLLPEE161 pKa = 4.46MIDD164 pKa = 3.39RR165 pKa = 11.84RR166 pKa = 11.84MTKK169 pKa = 9.97RR170 pKa = 11.84NLYY173 pKa = 10.91NEE175 pKa = 4.03MFFINRR181 pKa = 11.84TNIHH185 pKa = 6.99LIKK188 pKa = 10.68DD189 pKa = 3.55GHH191 pKa = 5.1TTNSSGHH198 pKa = 5.97DD199 pKa = 3.25LKK201 pKa = 11.42YY202 pKa = 10.46WNTAFARR209 pKa = 11.84QHH211 pKa = 5.93LVEE214 pKa = 4.75SHH216 pKa = 7.31DD217 pKa = 3.56EE218 pKa = 4.02DD219 pKa = 4.94KK220 pKa = 11.12IRR222 pKa = 11.84LVFGAPSTFLMAEE235 pKa = 4.16LTFIWPLQTSLLYY248 pKa = 10.29QGEE251 pKa = 4.54RR252 pKa = 11.84SPMLWGYY259 pKa = 7.61EE260 pKa = 4.06TTTGGWSRR268 pKa = 11.84LYY270 pKa = 10.17KK271 pKa = 9.2WASSRR276 pKa = 11.84MPRR279 pKa = 11.84YY280 pKa = 10.44DD281 pKa = 4.48FVATLDD287 pKa = 3.34WKK289 pKa = 11.1RR290 pKa = 11.84FDD292 pKa = 3.38RR293 pKa = 11.84DD294 pKa = 3.05ARR296 pKa = 11.84HH297 pKa = 5.32TVISDD302 pKa = 2.41IHH304 pKa = 6.5QLVMRR309 pKa = 11.84SYY311 pKa = 10.94FDD313 pKa = 3.88FNNGYY318 pKa = 9.49HH319 pKa = 5.75PTIHH323 pKa = 6.45YY324 pKa = 8.35PDD326 pKa = 3.51STGANPQRR334 pKa = 11.84IEE336 pKa = 4.37NLWNWMTDD344 pKa = 2.92ATLTIPLMLPDD355 pKa = 4.06GKK357 pKa = 10.18ILRR360 pKa = 11.84FKK362 pKa = 11.0HH363 pKa = 5.34SGIYY367 pKa = 9.75SGYY370 pKa = 9.65FQTQILDD377 pKa = 3.45SMYY380 pKa = 8.75NCVMIFTVLSRR391 pKa = 11.84MGFDD395 pKa = 3.96LEE397 pKa = 4.04RR398 pKa = 11.84VEE400 pKa = 6.0IKK402 pKa = 10.9VQGDD406 pKa = 3.3DD407 pKa = 5.2SIFLMCYY414 pKa = 10.07PFITLQNTFLQMFAHH429 pKa = 6.2YY430 pKa = 10.37AKK432 pKa = 9.96IYY434 pKa = 10.36FGSTLNIDD442 pKa = 3.42KK443 pKa = 11.22SEE445 pKa = 4.02ILPSLEE451 pKa = 3.63NAEE454 pKa = 4.02VLKK457 pKa = 10.66YY458 pKa = 10.13RR459 pKa = 11.84NHH461 pKa = 5.17GTMPYY466 pKa = 9.82RR467 pKa = 11.84EE468 pKa = 4.31EE469 pKa = 4.05LQLLAMLRR477 pKa = 11.84HH478 pKa = 6.21PEE480 pKa = 4.06RR481 pKa = 11.84TVSLPSLMARR491 pKa = 11.84SIGIAYY497 pKa = 8.69ANCGFHH503 pKa = 6.63SRR505 pKa = 11.84VYY507 pKa = 9.7QICEE511 pKa = 3.99DD512 pKa = 3.21IYY514 pKa = 11.58NFLKK518 pKa = 10.89AGGYY522 pKa = 9.92SPDD525 pKa = 3.43PHH527 pKa = 6.89GLPGSLRR534 pKa = 11.84YY535 pKa = 8.9RR536 pKa = 11.84QNYY539 pKa = 7.79VPGYY543 pKa = 8.46SEE545 pKa = 4.5VDD547 pKa = 2.81ISHH550 pKa = 6.73FPSYY554 pKa = 10.96FEE556 pKa = 4.13TVRR559 pKa = 11.84LLQEE563 pKa = 3.74PTRR566 pKa = 11.84DD567 pKa = 3.42LVSEE571 pKa = 3.99KK572 pKa = 10.13HH573 pKa = 5.39WPLKK577 pKa = 10.56HH578 pKa = 6.82FIGIPGKK585 pKa = 10.15SS586 pKa = 3.04

Molecular weight: 68.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1077

491

586

538.5

61.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.985 ± 3.116

1.207 ± 0.42

5.478 ± 0.396

5.014 ± 0.085

5.664 ± 0.163

4.921 ± 0.432

3.435 ± 0.392

6.035 ± 0.497

4.55 ± 0.32

9.935 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.304

4.921 ± 0.022

6.778 ± 1.192

2.971 ± 0.056

5.385 ± 0.744

7.707 ± 0.159

5.2 ± 0.619

3.714 ± 0.104

1.486 ± 0.45

4.921 ± 1.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski