Amino acid dipepetide frequency for Bacillus phage 031MP002

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.169AlaAla: 6.169 ± 0.795
0.461AlaCys: 0.461 ± 0.154
4.613AlaAsp: 4.613 ± 0.592
6.285AlaGlu: 6.285 ± 0.712
2.768AlaPhe: 2.768 ± 0.363
4.036AlaGly: 4.036 ± 0.63
0.519AlaHis: 0.519 ± 0.173
4.497AlaIle: 4.497 ± 0.605
5.189AlaLys: 5.189 ± 0.54
6.804AlaLeu: 6.804 ± 0.829
2.076AlaMet: 2.076 ± 0.301
4.382AlaAsn: 4.382 ± 0.582
1.845AlaPro: 1.845 ± 0.288
3.056AlaGln: 3.056 ± 0.57
2.825AlaArg: 2.825 ± 0.458
3.113AlaSer: 3.113 ± 0.369
4.094AlaThr: 4.094 ± 0.628
6.169AlaVal: 6.169 ± 0.994
0.98AlaTrp: 0.98 ± 0.312
2.133AlaTyr: 2.133 ± 0.405
0.0AlaXaa: 0.0 ± 0.0
Cys
0.346CysAla: 0.346 ± 0.137
0.115CysCys: 0.115 ± 0.094
0.231CysAsp: 0.231 ± 0.115
0.692CysGlu: 0.692 ± 0.227
0.288CysPhe: 0.288 ± 0.134
0.519CysGly: 0.519 ± 0.204
0.288CysHis: 0.288 ± 0.109
0.231CysIle: 0.231 ± 0.116
0.404CysLys: 0.404 ± 0.153
0.634CysLeu: 0.634 ± 0.186
0.231CysMet: 0.231 ± 0.107
0.346CysAsn: 0.346 ± 0.127
0.346CysPro: 0.346 ± 0.137
0.346CysGln: 0.346 ± 0.159
0.346CysArg: 0.346 ± 0.144
0.058CysSer: 0.058 ± 0.067
0.404CysThr: 0.404 ± 0.236
0.288CysVal: 0.288 ± 0.097
0.058CysTrp: 0.058 ± 0.054
0.058CysTyr: 0.058 ± 0.052
0.0CysXaa: 0.0 ± 0.0
Asp
3.921AspAla: 3.921 ± 0.363
0.577AspCys: 0.577 ± 0.246
3.286AspAsp: 3.286 ± 0.451
5.477AspGlu: 5.477 ± 0.834
2.364AspPhe: 2.364 ± 0.343
4.44AspGly: 4.44 ± 0.474
1.038AspHis: 1.038 ± 0.209
4.901AspIle: 4.901 ± 0.6
3.632AspLys: 3.632 ± 0.535
4.209AspLeu: 4.209 ± 0.457
2.249AspMet: 2.249 ± 0.373
3.113AspAsn: 3.113 ± 0.412
2.249AspPro: 2.249 ± 0.412
1.672AspGln: 1.672 ± 0.257
2.71AspArg: 2.71 ± 0.333
2.652AspSer: 2.652 ± 0.354
3.286AspThr: 3.286 ± 0.44
4.44AspVal: 4.44 ± 0.489
0.75AspTrp: 0.75 ± 0.227
2.364AspTyr: 2.364 ± 0.398
0.0AspXaa: 0.0 ± 0.0
Glu
5.593GluAla: 5.593 ± 0.614
0.98GluCys: 0.98 ± 0.23
4.555GluAsp: 4.555 ± 0.683
8.879GluGlu: 8.879 ± 1.077
2.768GluPhe: 2.768 ± 0.475
6.4GluGly: 6.4 ± 0.651
1.211GluHis: 1.211 ± 0.287
4.786GluIle: 4.786 ± 0.474
5.708GluLys: 5.708 ± 0.71
7.265GluLeu: 7.265 ± 0.895
2.768GluMet: 2.768 ± 0.492
4.324GluAsn: 4.324 ± 0.524
1.845GluPro: 1.845 ± 0.374
2.652GluGln: 2.652 ± 0.48
5.131GluArg: 5.131 ± 0.627
4.843GluSer: 4.843 ± 0.616
4.44GluThr: 4.44 ± 0.542
4.958GluVal: 4.958 ± 0.628
1.268GluTrp: 1.268 ± 0.28
3.286GluTyr: 3.286 ± 0.394
0.0GluXaa: 0.0 ± 0.0
Phe
2.133PheAla: 2.133 ± 0.356
0.231PheCys: 0.231 ± 0.125
2.249PheAsp: 2.249 ± 0.348
2.652PheGlu: 2.652 ± 0.345
1.499PhePhe: 1.499 ± 0.386
3.171PheGly: 3.171 ± 0.398
1.211PheHis: 1.211 ± 0.292
2.191PheIle: 2.191 ± 0.278
2.537PheLys: 2.537 ± 0.376
2.652PheLeu: 2.652 ± 0.422
1.038PheMet: 1.038 ± 0.218
1.845PheAsn: 1.845 ± 0.356
1.499PhePro: 1.499 ± 0.335
1.499PheGln: 1.499 ± 0.323
1.672PheArg: 1.672 ± 0.33
2.076PheSer: 2.076 ± 0.385
3.344PheThr: 3.344 ± 0.482
2.076PheVal: 2.076 ± 0.309
0.404PheTrp: 0.404 ± 0.156
1.787PheTyr: 1.787 ± 0.292
0.0PheXaa: 0.0 ± 0.0
Gly
4.094GlyAla: 4.094 ± 0.715
0.461GlyCys: 0.461 ± 0.167
4.44GlyAsp: 4.44 ± 0.476
5.996GlyGlu: 5.996 ± 0.655
3.344GlyPhe: 3.344 ± 0.522
5.074GlyGly: 5.074 ± 0.734
1.211GlyHis: 1.211 ± 0.243
4.786GlyIle: 4.786 ± 0.67
4.901GlyLys: 4.901 ± 0.554
5.65GlyLeu: 5.65 ± 0.995
2.422GlyMet: 2.422 ± 0.403
3.748GlyAsn: 3.748 ± 0.648
0.058GlyPro: 0.058 ± 0.05
2.076GlyGln: 2.076 ± 0.357
3.517GlyArg: 3.517 ± 0.427
4.324GlySer: 4.324 ± 0.639
4.036GlyThr: 4.036 ± 0.681
5.708GlyVal: 5.708 ± 0.626
0.807GlyTrp: 0.807 ± 0.255
3.171GlyTyr: 3.171 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
0.807HisAla: 0.807 ± 0.214
0.115HisCys: 0.115 ± 0.089
0.865HisAsp: 0.865 ± 0.214
0.98HisGlu: 0.98 ± 0.234
0.692HisPhe: 0.692 ± 0.184
0.923HisGly: 0.923 ± 0.252
0.577HisHis: 0.577 ± 0.217
1.557HisIle: 1.557 ± 0.277
1.614HisLys: 1.614 ± 0.288
0.98HisLeu: 0.98 ± 0.257
0.404HisMet: 0.404 ± 0.136
0.692HisAsn: 0.692 ± 0.197
0.692HisPro: 0.692 ± 0.189
0.346HisGln: 0.346 ± 0.128
0.923HisArg: 0.923 ± 0.215
0.577HisSer: 0.577 ± 0.173
1.153HisThr: 1.153 ± 0.281
1.268HisVal: 1.268 ± 0.216
0.346HisTrp: 0.346 ± 0.11
0.807HisTyr: 0.807 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
3.805IleAla: 3.805 ± 0.553
0.346IleCys: 0.346 ± 0.133
4.728IleAsp: 4.728 ± 0.545
6.112IleGlu: 6.112 ± 0.809
2.076IlePhe: 2.076 ± 0.343
4.036IleGly: 4.036 ± 0.545
1.557IleHis: 1.557 ± 0.28
4.209IleIle: 4.209 ± 0.507
5.881IleLys: 5.881 ± 0.784
4.497IleLeu: 4.497 ± 0.442
1.787IleMet: 1.787 ± 0.359
2.94IleAsn: 2.94 ± 0.442
2.537IlePro: 2.537 ± 0.337
2.94IleGln: 2.94 ± 0.382
3.459IleArg: 3.459 ± 0.453
3.748IleSer: 3.748 ± 0.464
4.843IleThr: 4.843 ± 0.678
3.921IleVal: 3.921 ± 0.444
0.519IleTrp: 0.519 ± 0.224
2.133IleTyr: 2.133 ± 0.385
0.0IleXaa: 0.0 ± 0.0
Lys
6.631LysAla: 6.631 ± 0.694
0.404LysCys: 0.404 ± 0.145
3.459LysAsp: 3.459 ± 0.438
6.804LysGlu: 6.804 ± 0.894
2.422LysPhe: 2.422 ± 0.342
6.054LysGly: 6.054 ± 0.633
1.268LysHis: 1.268 ± 0.253
3.978LysIle: 3.978 ± 0.411
7.726LysLys: 7.726 ± 0.955
4.958LysLeu: 4.958 ± 0.66
1.903LysMet: 1.903 ± 0.365
4.613LysAsn: 4.613 ± 0.534
2.768LysPro: 2.768 ± 0.481
2.595LysGln: 2.595 ± 0.346
3.921LysArg: 3.921 ± 0.561
3.229LysSer: 3.229 ± 0.567
5.766LysThr: 5.766 ± 0.713
5.016LysVal: 5.016 ± 0.532
0.98LysTrp: 0.98 ± 0.234
3.459LysTyr: 3.459 ± 0.502
0.0LysXaa: 0.0 ± 0.0
Leu
5.304LeuAla: 5.304 ± 0.682
0.288LeuCys: 0.288 ± 0.125
5.477LeuAsp: 5.477 ± 0.534
6.746LeuGlu: 6.746 ± 0.72
2.133LeuPhe: 2.133 ± 0.266
5.362LeuGly: 5.362 ± 0.698
0.634LeuHis: 0.634 ± 0.188
4.44LeuIle: 4.44 ± 0.517
6.688LeuLys: 6.688 ± 0.833
4.613LeuLeu: 4.613 ± 0.493
2.249LeuMet: 2.249 ± 0.326
4.209LeuAsn: 4.209 ± 0.513
2.306LeuPro: 2.306 ± 0.387
3.113LeuGln: 3.113 ± 0.4
3.286LeuArg: 3.286 ± 0.414
5.247LeuSer: 5.247 ± 0.579
5.477LeuThr: 5.477 ± 0.373
4.382LeuVal: 4.382 ± 0.431
0.692LeuTrp: 0.692 ± 0.203
2.768LeuTyr: 2.768 ± 0.32
0.0LeuXaa: 0.0 ± 0.0
Met
2.422MetAla: 2.422 ± 0.419
0.058MetCys: 0.058 ± 0.052
1.672MetAsp: 1.672 ± 0.212
1.557MetGlu: 1.557 ± 0.271
0.807MetPhe: 0.807 ± 0.221
1.614MetGly: 1.614 ± 0.427
0.173MetHis: 0.173 ± 0.097
2.306MetIle: 2.306 ± 0.439
2.825MetLys: 2.825 ± 0.473
1.96MetLeu: 1.96 ± 0.349
0.75MetMet: 0.75 ± 0.213
1.845MetAsn: 1.845 ± 0.38
1.326MetPro: 1.326 ± 0.224
0.75MetGln: 0.75 ± 0.222
1.73MetArg: 1.73 ± 0.264
1.614MetSer: 1.614 ± 0.24
2.364MetThr: 2.364 ± 0.336
1.153MetVal: 1.153 ± 0.231
0.692MetTrp: 0.692 ± 0.23
0.577MetTyr: 0.577 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
3.978AsnAla: 3.978 ± 0.792
0.461AsnCys: 0.461 ± 0.168
3.286AsnAsp: 3.286 ± 0.556
3.459AsnGlu: 3.459 ± 0.403
1.787AsnPhe: 1.787 ± 0.29
4.44AsnGly: 4.44 ± 0.654
0.98AsnHis: 0.98 ± 0.211
3.459AsnIle: 3.459 ± 0.509
3.459AsnLys: 3.459 ± 0.452
3.978AsnLeu: 3.978 ± 0.498
0.923AsnMet: 0.923 ± 0.228
2.422AsnAsn: 2.422 ± 0.301
2.364AsnPro: 2.364 ± 0.346
1.672AsnGln: 1.672 ± 0.262
2.422AsnArg: 2.422 ± 0.432
3.286AsnSer: 3.286 ± 0.414
2.768AsnThr: 2.768 ± 0.693
3.575AsnVal: 3.575 ± 0.388
0.98AsnTrp: 0.98 ± 0.184
1.96AsnTyr: 1.96 ± 0.288
0.0AsnXaa: 0.0 ± 0.0
Pro
2.249ProAla: 2.249 ± 0.306
0.346ProCys: 0.346 ± 0.15
1.672ProAsp: 1.672 ± 0.288
3.286ProGlu: 3.286 ± 0.544
1.614ProPhe: 1.614 ± 0.328
0.115ProGly: 0.115 ± 0.069
0.404ProHis: 0.404 ± 0.168
1.672ProIle: 1.672 ± 0.277
2.306ProLys: 2.306 ± 0.41
2.595ProLeu: 2.595 ± 0.323
0.634ProMet: 0.634 ± 0.168
1.614ProAsn: 1.614 ± 0.324
1.614ProPro: 1.614 ± 0.334
1.557ProGln: 1.557 ± 0.334
0.923ProArg: 0.923 ± 0.261
2.364ProSer: 2.364 ± 0.321
2.364ProThr: 2.364 ± 0.404
1.845ProVal: 1.845 ± 0.272
0.173ProTrp: 0.173 ± 0.1
1.153ProTyr: 1.153 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
3.459GlnAla: 3.459 ± 0.561
0.173GlnCys: 0.173 ± 0.118
2.018GlnAsp: 2.018 ± 0.285
3.748GlnGlu: 3.748 ± 0.52
0.98GlnPhe: 0.98 ± 0.228
2.364GlnGly: 2.364 ± 0.405
0.288GlnHis: 0.288 ± 0.109
2.133GlnIle: 2.133 ± 0.314
3.459GlnLys: 3.459 ± 0.503
2.71GlnLeu: 2.71 ± 0.419
1.211GlnMet: 1.211 ± 0.29
2.018GlnAsn: 2.018 ± 0.334
1.153GlnPro: 1.153 ± 0.225
1.441GlnGln: 1.441 ± 0.364
1.499GlnArg: 1.499 ± 0.305
1.268GlnSer: 1.268 ± 0.252
1.96GlnThr: 1.96 ± 0.308
2.479GlnVal: 2.479 ± 0.405
0.519GlnTrp: 0.519 ± 0.155
1.326GlnTyr: 1.326 ± 0.314
0.0GlnXaa: 0.0 ± 0.0
Arg
3.632ArgAla: 3.632 ± 0.46
0.058ArgCys: 0.058 ± 0.057
2.998ArgAsp: 2.998 ± 0.348
4.151ArgGlu: 4.151 ± 0.549
1.845ArgPhe: 1.845 ± 0.342
2.883ArgGly: 2.883 ± 0.37
0.807ArgHis: 0.807 ± 0.25
3.632ArgIle: 3.632 ± 0.406
3.69ArgLys: 3.69 ± 0.504
3.805ArgLeu: 3.805 ± 0.537
1.614ArgMet: 1.614 ± 0.322
2.191ArgAsn: 2.191 ± 0.398
1.384ArgPro: 1.384 ± 0.231
2.249ArgGln: 2.249 ± 0.426
3.402ArgArg: 3.402 ± 0.668
2.249ArgSer: 2.249 ± 0.317
3.113ArgThr: 3.113 ± 0.432
3.171ArgVal: 3.171 ± 0.508
0.404ArgTrp: 0.404 ± 0.149
2.422ArgTyr: 2.422 ± 0.435
0.0ArgXaa: 0.0 ± 0.0
Ser
3.632SerAla: 3.632 ± 0.578
0.058SerCys: 0.058 ± 0.056
3.171SerAsp: 3.171 ± 0.309
3.69SerGlu: 3.69 ± 0.493
2.479SerPhe: 2.479 ± 0.366
5.593SerGly: 5.593 ± 0.568
0.807SerHis: 0.807 ± 0.221
3.978SerIle: 3.978 ± 0.496
4.44SerLys: 4.44 ± 0.443
4.267SerLeu: 4.267 ± 0.475
1.384SerMet: 1.384 ± 0.292
2.306SerAsn: 2.306 ± 0.343
0.98SerPro: 0.98 ± 0.273
1.787SerGln: 1.787 ± 0.296
2.479SerArg: 2.479 ± 0.396
3.575SerSer: 3.575 ± 0.845
3.517SerThr: 3.517 ± 0.547
3.69SerVal: 3.69 ± 0.443
0.634SerTrp: 0.634 ± 0.213
2.133SerTyr: 2.133 ± 0.436
0.0SerXaa: 0.0 ± 0.0
Thr
5.304ThrAla: 5.304 ± 1.063
0.346ThrCys: 0.346 ± 0.166
3.632ThrAsp: 3.632 ± 0.459
4.267ThrGlu: 4.267 ± 0.574
3.113ThrPhe: 3.113 ± 0.385
5.362ThrGly: 5.362 ± 0.606
0.865ThrHis: 0.865 ± 0.288
5.42ThrIle: 5.42 ± 0.603
4.094ThrLys: 4.094 ± 0.499
6.285ThrLeu: 6.285 ± 0.577
1.672ThrMet: 1.672 ± 0.226
2.537ThrAsn: 2.537 ± 0.424
2.249ThrPro: 2.249 ± 0.432
2.422ThrGln: 2.422 ± 0.347
2.71ThrArg: 2.71 ± 0.327
2.883ThrSer: 2.883 ± 0.492
4.44ThrThr: 4.44 ± 0.901
5.247ThrVal: 5.247 ± 0.592
0.634ThrTrp: 0.634 ± 0.215
2.249ThrTyr: 2.249 ± 0.418
0.0ThrXaa: 0.0 ± 0.0
Val
4.901ValAla: 4.901 ± 0.489
0.346ValCys: 0.346 ± 0.165
3.459ValAsp: 3.459 ± 0.454
4.843ValGlu: 4.843 ± 0.478
3.113ValPhe: 3.113 ± 0.342
4.497ValGly: 4.497 ± 0.511
1.211ValHis: 1.211 ± 0.266
4.209ValIle: 4.209 ± 0.461
4.843ValLys: 4.843 ± 0.434
4.382ValLeu: 4.382 ± 0.465
1.672ValMet: 1.672 ± 0.276
3.171ValAsn: 3.171 ± 0.406
2.076ValPro: 2.076 ± 0.308
2.71ValGln: 2.71 ± 0.345
4.094ValArg: 4.094 ± 0.562
4.209ValSer: 4.209 ± 0.412
5.016ValThr: 5.016 ± 0.761
4.267ValVal: 4.267 ± 0.503
0.923ValTrp: 0.923 ± 0.269
3.286ValTyr: 3.286 ± 0.51
0.0ValXaa: 0.0 ± 0.0
Trp
1.153TrpAla: 1.153 ± 0.202
0.115TrpCys: 0.115 ± 0.081
0.75TrpAsp: 0.75 ± 0.189
0.75TrpGlu: 0.75 ± 0.22
0.461TrpPhe: 0.461 ± 0.161
0.634TrpGly: 0.634 ± 0.177
0.404TrpHis: 0.404 ± 0.151
0.807TrpIle: 0.807 ± 0.22
1.326TrpLys: 1.326 ± 0.289
0.75TrpLeu: 0.75 ± 0.194
0.346TrpMet: 0.346 ± 0.158
1.384TrpAsn: 1.384 ± 0.513
0.0TrpPro: 0.0 ± 0.0
0.231TrpGln: 0.231 ± 0.11
0.692TrpArg: 0.692 ± 0.194
0.75TrpSer: 0.75 ± 0.2
0.923TrpThr: 0.923 ± 0.239
0.634TrpVal: 0.634 ± 0.176
0.058TrpTrp: 0.058 ± 0.052
0.231TrpTyr: 0.231 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.479TyrAla: 2.479 ± 0.366
0.288TyrCys: 0.288 ± 0.112
2.768TyrAsp: 2.768 ± 0.449
3.286TyrGlu: 3.286 ± 0.52
1.211TyrPhe: 1.211 ± 0.267
2.306TyrGly: 2.306 ± 0.406
0.865TyrHis: 0.865 ± 0.224
2.883TyrIle: 2.883 ± 0.439
3.229TyrLys: 3.229 ± 0.534
2.479TyrLeu: 2.479 ± 0.518
0.807TyrMet: 0.807 ± 0.217
1.96TyrAsn: 1.96 ± 0.304
1.211TyrPro: 1.211 ± 0.316
1.095TyrGln: 1.095 ± 0.226
1.96TyrArg: 1.96 ± 0.309
2.537TyrSer: 2.537 ± 0.338
2.422TyrThr: 2.422 ± 0.364
2.94TyrVal: 2.94 ± 0.472
0.577TyrTrp: 0.577 ± 0.173
1.903TyrTyr: 1.903 ± 0.448
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (17345 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski